YLR056W / ERG3

C-5 sterol desaturase, glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of the HRD ubiquitin ligase

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR056W / ERG3

Click on Significant Values for Screen Details ID:SGTC_442|Compound:cape|FD-Score:3.99|P-value:7.63E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:4.31|P-value:3.13E-4 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:4.25|P-value:3.68E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:-3.99|P-value:7.64E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:4.05|P-value:6.56E-4 ID:SGTC_1526|Compound:st051518|FD-Score:3.93|P-value:9.06E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:4.16|P-value:4.81E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:4.07|P-value:6.27E-4 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:4.33|P-value:2.96E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.93|P-value:9.20E-4 ID:SGTC_1759|Compound:st043059|FD-Score:-4.48|P-value:1.85E-4 ID:SGTC_1766|Compound:st045414|FD-Score:4.06|P-value:6.44E-4 ID:SGTC_1780|Compound:st038448|FD-Score:4.05|P-value:6.57E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.34|P-value:2.81E-4 ID:SGTC_1921|Compound:5740136|FD-Score:-4.45|P-value:2.04E-4 ID:SGTC_1923|Compound:st058458|FD-Score:4.26|P-value:3.65E-4 ID:SGTC_1975|Compound:st070967|FD-Score:3.90|P-value:9.78E-4 ID:SGTC_2170|Compound:morpholine|FD-Score:-3.93|P-value:8.88E-4 ID:SGTC_2434|Compound:5670830|FD-Score:4.18|P-value:4.54E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:4.27|P-value:3.50E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-5.18|P-value:1.92E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:-4.67|P-value:1.03E-4 ID:SGTC_2729|Compound:fluphenazine|FD-Score:-4.24|P-value:3.74E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-5.32|P-value:1.18E-5 ID:SGTC_3152|Compound:9099253|FD-Score:-4.37|P-value:2.58E-4 ID:SGTC_3242|Compound:9133940|FD-Score:4.03|P-value:6.94E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-4.01|P-value:7.26E-4 ID:SGTC_3286|Compound:9117135|FD-Score:-4.40|P-value:2.36E-4 ID:SGTC_3302|Compound:9125395|FD-Score:-3.92|P-value:9.15E-4 ID:SGTC_3310|Compound:9130229|FD-Score:-4.87|P-value:5.48E-5 ID:SGTC_442|Compound:cape|FD-Score:3.99|P-value:7.63E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:4.31|P-value:3.13E-4 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:4.25|P-value:3.68E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:-3.99|P-value:7.64E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:4.05|P-value:6.56E-4 ID:SGTC_1526|Compound:st051518|FD-Score:3.93|P-value:9.06E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:4.16|P-value:4.81E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:4.07|P-value:6.27E-4 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:4.33|P-value:2.96E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.93|P-value:9.20E-4 ID:SGTC_1759|Compound:st043059|FD-Score:-4.48|P-value:1.85E-4 ID:SGTC_1766|Compound:st045414|FD-Score:4.06|P-value:6.44E-4 ID:SGTC_1780|Compound:st038448|FD-Score:4.05|P-value:6.57E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.34|P-value:2.81E-4 ID:SGTC_1921|Compound:5740136|FD-Score:-4.45|P-value:2.04E-4 ID:SGTC_1923|Compound:st058458|FD-Score:4.26|P-value:3.65E-4 ID:SGTC_1975|Compound:st070967|FD-Score:3.90|P-value:9.78E-4 ID:SGTC_2170|Compound:morpholine|FD-Score:-3.93|P-value:8.88E-4 ID:SGTC_2434|Compound:5670830|FD-Score:4.18|P-value:4.54E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:4.27|P-value:3.50E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-5.18|P-value:1.92E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:-4.67|P-value:1.03E-4 ID:SGTC_2729|Compound:fluphenazine|FD-Score:-4.24|P-value:3.74E-4 ID:SGTC_2777|Compound:climbazole|FD-Score:-5.32|P-value:1.18E-5 ID:SGTC_3152|Compound:9099253|FD-Score:-4.37|P-value:2.58E-4 ID:SGTC_3242|Compound:9133940|FD-Score:4.03|P-value:6.94E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-4.01|P-value:7.26E-4 ID:SGTC_3286|Compound:9117135|FD-Score:-4.40|P-value:2.36E-4 ID:SGTC_3302|Compound:9125395|FD-Score:-3.92|P-value:9.15E-4 ID:SGTC_3310|Compound:9130229|FD-Score:-4.87|P-value:5.48E-5

Top fitness defect scores for YLR056W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1537 2',6'-dihydroxyflavanone 4.33 78.0 µM 2.96E-4 13 7.50E-6
2 SGTC_450 aminopyrene 4.31 156.0 nM 3.13E-4 25 8.19E-6
3 SGTC_2544 sphondin 4.27 68.5 µM 3.50E-4 RNA pol III & RNase P/MRP 19 9.75E-6
4 SGTC_1923 st058458 4.26 19.1 µM 3.65E-4 26 1.04E-5
5 SGTC_1116 jasmonic acid 4.25 1.6 µM 3.68E-4 17 1.05E-5
6 SGTC_2434 5670830 4.18 169.9 µM 4.54E-4 20 1.46E-5
7 SGTC_1527 coumarin 4.16 137.0 µM 4.81E-4 19 1.59E-5
8 SGTC_1529 apigenin 4.07 74.0 µM 6.27E-4 32 2.40E-5
9 SGTC_1766 st045414 4.06 44.8 µM 6.44E-4 RPP1 & pyrimidine depletion 23 2.50E-5
10 SGTC_1520 flindersine 4.05 88.0 µM 6.56E-4 28 2.57E-5
11 SGTC_1780 st038448 4.05 24.5 µM 6.57E-4 iron homeostasis 14 2.58E-5
12 SGTC_3242 9133940 4.03 49.5 µM 6.94E-4 11 2.81E-5
13 SGTC_442 cape 3.99 100.0 µM 7.63E-4 16 3.25E-5
14 SGTC_1526 st051518 3.93 75.0 µM 9.06E-4 20 4.24E-5
15 SGTC_1568 digitonin 3.93 820.0 nM 9.20E-4 sphingolipid biosynthesis & PDR1 33 4.34E-5
16 SGTC_1975 st070967 3.90 30.8 µM 9.78E-4 33 4.76E-5
17 SGTC_1118 chelidonine 3.81 707.5 nM 0.00126 17 7.01E-5
18 SGTC_861 0443-0273 3.74 110.0 µM 0.00150 14 9.19E-5
19 SGTC_2519 cryptotanshinone 3.65 88.0 µM 0.00190 heme biosynthesis & mitochondrial translocase 21 1.32E-4
20 SGTC_598 3937-0236 3.63 20.2 µM 0.00200 ERG2 38 1.43E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.104 1.74E-9 YLR148W PEP3 hom Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis
0.103 2.14E-9 YJL117W PHO86 hom Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress
0.096 3.00E-8 YCR046C IMG1 hom Mitochondrial ribosomal protein of the large subunit, required for respiration and for maintenance of the mitochondrial genome
0.088 2.92E-7 YBR241C_p YBR241C_p hom Putative transporter, member of the sugar porter family; green fluorescent protein (GFP)-fusion protein localizes to the vacuolar membrane; YBR241C is not an essential gene
0.085 7.97E-7 YER050C RSM18 hom Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein
0.081 2.48E-6 YNL177C MRPL22 hom Mitochondrial ribosomal protein of the large subunit
0.079 4.55E-6 YJL168C SET2 hom Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
0.078 6.25E-6 YNL087W TCB2 hom ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains 3 calcium and lipid binding domains; mRNA is targeted to the bud
0.078 6.92E-6 YPL236C ENV7 hom Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)
0.077 7.75E-6 YMR172W HOT1 hom Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p
0.077 8.41E-6 YLR439W MRPL4 hom Mitochondrial ribosomal protein of the large subunit, homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit
0.075 1.29E-5 YLR069C MEF1 hom Mitochondrial elongation factor involved in translational elongation
0.075 1.36E-5 YOR034C-A_p YOR034C-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.072 3.05E-5 YHL031C GOS1 hom v-SNARE protein involved in Golgi transport, homolog of the mammalian protein GOS-28/GS28
0.072 3.33E-5 YER087C-A_d YER087C-A_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase