YLR057W / MNL2

Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR057W / MNL2

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:5.89|P-value:9.82E-9 ID:SGTC_18|Compound:0111-0177|FD-Score:3.49|P-value:4.50E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:4.32|P-value:1.94E-5 ID:SGTC_221|Compound:trifluoperazine|FD-Score:14.30|P-value:6.10E-43 ID:SGTC_232|Compound:clozapine|FD-Score:6.56|P-value:1.96E-10 ID:SGTC_301|Compound:4119-0100|FD-Score:3.91|P-value:9.91E-5 ID:SGTC_313|Compound:3474-0040|FD-Score:4.34|P-value:1.80E-5 ID:SGTC_384|Compound:k007-0144|FD-Score:8.60|P-value:1.12E-16 ID:SGTC_427|Compound:1354-0053|FD-Score:4.21|P-value:2.99E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.46|P-value:5.02E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:3.56|P-value:3.45E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:5.72|P-value:2.49E-8 ID:SGTC_552|Compound:k242-0005|FD-Score:3.36|P-value:6.84E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:9.34|P-value:2.33E-19 ID:SGTC_637|Compound:1181-0519|FD-Score:10.70|P-value:8.96E-25 ID:SGTC_733|Compound:4069-0007|FD-Score:4.20|P-value:3.13E-5 ID:SGTC_768|Compound:0384-0033|FD-Score:3.75|P-value:1.75E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:3.54|P-value:3.77E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:4.26|P-value:2.44E-5 ID:SGTC_1060|Compound:3448-7350|FD-Score:-4.46|P-value:9.49E-6 ID:SGTC_1063|Compound:disulfiram|FD-Score:4.54|P-value:7.44E-6 ID:SGTC_1125|Compound:tnp00248|FD-Score:-3.63|P-value:2.46E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:-3.26|P-value:8.54E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:6.54|P-value:2.21E-10 ID:SGTC_1205|Compound:4161-2785|FD-Score:4.04|P-value:5.92E-5 ID:SGTC_1526|Compound:st051518|FD-Score:-4.08|P-value:4.57E-5 ID:SGTC_1530|Compound:rhein|FD-Score:3.32|P-value:7.94E-4 ID:SGTC_1619|Compound:st003326|FD-Score:4.09|P-value:4.96E-5 ID:SGTC_1712|Compound:st032288|FD-Score:4.16|P-value:3.70E-5 ID:SGTC_1713|Compound:st033146|FD-Score:6.94|P-value:1.73E-11 ID:SGTC_1721|Compound:st082152|FD-Score:4.52|P-value:8.11E-6 ID:SGTC_1724|Compound:st037281|FD-Score:4.54|P-value:7.57E-6 ID:SGTC_1725|Compound:st036284|FD-Score:-3.40|P-value:5.37E-4 ID:SGTC_1754|Compound:st042065|FD-Score:4.53|P-value:7.83E-6 ID:SGTC_1760|Compound:st045453|FD-Score:4.19|P-value:3.34E-5 ID:SGTC_1781|Compound:st038725|FD-Score:10.90|P-value:1.58E-25 ID:SGTC_1800|Compound:st050079|FD-Score:7.19|P-value:3.53E-12 ID:SGTC_1801|Compound:st050089|FD-Score:3.41|P-value:5.92E-4 ID:SGTC_1803|Compound:st050068|FD-Score:6.08|P-value:3.25E-9 ID:SGTC_1818|Compound:st052136|FD-Score:5.37|P-value:1.54E-7 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:3.32|P-value:7.78E-4 ID:SGTC_1879|Compound:gemcitabine|FD-Score:3.38|P-value:6.52E-4 ID:SGTC_1954|Compound:st077032|FD-Score:4.97|P-value:1.10E-6 ID:SGTC_1991|Compound:st073000|FD-Score:5.73|P-value:2.36E-8 ID:SGTC_2178|Compound:5812956|FD-Score:3.41|P-value:5.83E-4 ID:SGTC_2191|Compound:5911152|FD-Score:3.96|P-value:8.16E-5 ID:SGTC_2345|Compound:9052315|FD-Score:5.65|P-value:3.48E-8 ID:SGTC_2397|Compound:5161689|FD-Score:3.43|P-value:5.51E-4 ID:SGTC_2478|Compound:5763493|FD-Score:-3.88|P-value:9.86E-5 ID:SGTC_2480|Compound:5767542|FD-Score:-3.89|P-value:9.54E-5 ID:SGTC_2658|Compound:polymyxin b sulfate|FD-Score:6.18|P-value:1.89E-9 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.78|P-value:1.59E-4 ID:SGTC_2795|Compound:7780010|FD-Score:3.72|P-value:2.02E-4 ID:SGTC_2801|Compound:7907800|FD-Score:4.75|P-value:3.02E-6 ID:SGTC_2803|Compound:7707263|FD-Score:4.18|P-value:3.39E-5 ID:SGTC_2837|Compound:9008476|FD-Score:5.47|P-value:9.25E-8 ID:SGTC_2978|Compound:9011784|FD-Score:-3.75|P-value:1.58E-4 ID:SGTC_3037|Compound:9091026|FD-Score:8.59|P-value:1.21E-16 ID:SGTC_3056|Compound:9094900|FD-Score:-3.28|P-value:8.20E-4 ID:SGTC_3087|Compound:9117406|FD-Score:3.32|P-value:7.91E-4 ID:SGTC_3124|Compound:9127567|FD-Score:-3.95|P-value:7.49E-5 ID:SGTC_3152|Compound:9099253|FD-Score:15.90|P-value:3.76E-52 ID:SGTC_3159|Compound:9100096|FD-Score:3.81|P-value:1.42E-4 ID:SGTC_15|Compound:0266-0086|FD-Score:5.89|P-value:9.82E-9 ID:SGTC_18|Compound:0111-0177|FD-Score:3.49|P-value:4.50E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:4.32|P-value:1.94E-5 ID:SGTC_221|Compound:trifluoperazine|FD-Score:14.30|P-value:6.10E-43 ID:SGTC_232|Compound:clozapine|FD-Score:6.56|P-value:1.96E-10 ID:SGTC_301|Compound:4119-0100|FD-Score:3.91|P-value:9.91E-5 ID:SGTC_313|Compound:3474-0040|FD-Score:4.34|P-value:1.80E-5 ID:SGTC_384|Compound:k007-0144|FD-Score:8.60|P-value:1.12E-16 ID:SGTC_427|Compound:1354-0053|FD-Score:4.21|P-value:2.99E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.46|P-value:5.02E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:3.56|P-value:3.45E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:5.72|P-value:2.49E-8 ID:SGTC_552|Compound:k242-0005|FD-Score:3.36|P-value:6.84E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:9.34|P-value:2.33E-19 ID:SGTC_637|Compound:1181-0519|FD-Score:10.70|P-value:8.96E-25 ID:SGTC_733|Compound:4069-0007|FD-Score:4.20|P-value:3.13E-5 ID:SGTC_768|Compound:0384-0033|FD-Score:3.75|P-value:1.75E-4 ID:SGTC_900|Compound:1112-0045|FD-Score:3.54|P-value:3.77E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:4.26|P-value:2.44E-5 ID:SGTC_1060|Compound:3448-7350|FD-Score:-4.46|P-value:9.49E-6 ID:SGTC_1063|Compound:disulfiram|FD-Score:4.54|P-value:7.44E-6 ID:SGTC_1125|Compound:tnp00248|FD-Score:-3.63|P-value:2.46E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:-3.26|P-value:8.54E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:6.54|P-value:2.21E-10 ID:SGTC_1205|Compound:4161-2785|FD-Score:4.04|P-value:5.92E-5 ID:SGTC_1526|Compound:st051518|FD-Score:-4.08|P-value:4.57E-5 ID:SGTC_1530|Compound:rhein|FD-Score:3.32|P-value:7.94E-4 ID:SGTC_1619|Compound:st003326|FD-Score:4.09|P-value:4.96E-5 ID:SGTC_1712|Compound:st032288|FD-Score:4.16|P-value:3.70E-5 ID:SGTC_1713|Compound:st033146|FD-Score:6.94|P-value:1.73E-11 ID:SGTC_1721|Compound:st082152|FD-Score:4.52|P-value:8.11E-6 ID:SGTC_1724|Compound:st037281|FD-Score:4.54|P-value:7.57E-6 ID:SGTC_1725|Compound:st036284|FD-Score:-3.40|P-value:5.37E-4 ID:SGTC_1754|Compound:st042065|FD-Score:4.53|P-value:7.83E-6 ID:SGTC_1760|Compound:st045453|FD-Score:4.19|P-value:3.34E-5 ID:SGTC_1781|Compound:st038725|FD-Score:10.90|P-value:1.58E-25 ID:SGTC_1800|Compound:st050079|FD-Score:7.19|P-value:3.53E-12 ID:SGTC_1801|Compound:st050089|FD-Score:3.41|P-value:5.92E-4 ID:SGTC_1803|Compound:st050068|FD-Score:6.08|P-value:3.25E-9 ID:SGTC_1818|Compound:st052136|FD-Score:5.37|P-value:1.54E-7 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:3.32|P-value:7.78E-4 ID:SGTC_1879|Compound:gemcitabine|FD-Score:3.38|P-value:6.52E-4 ID:SGTC_1954|Compound:st077032|FD-Score:4.97|P-value:1.10E-6 ID:SGTC_1991|Compound:st073000|FD-Score:5.73|P-value:2.36E-8 ID:SGTC_2178|Compound:5812956|FD-Score:3.41|P-value:5.83E-4 ID:SGTC_2191|Compound:5911152|FD-Score:3.96|P-value:8.16E-5 ID:SGTC_2345|Compound:9052315|FD-Score:5.65|P-value:3.48E-8 ID:SGTC_2397|Compound:5161689|FD-Score:3.43|P-value:5.51E-4 ID:SGTC_2478|Compound:5763493|FD-Score:-3.88|P-value:9.86E-5 ID:SGTC_2480|Compound:5767542|FD-Score:-3.89|P-value:9.54E-5 ID:SGTC_2658|Compound:polymyxin b sulfate|FD-Score:6.18|P-value:1.89E-9 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.78|P-value:1.59E-4 ID:SGTC_2795|Compound:7780010|FD-Score:3.72|P-value:2.02E-4 ID:SGTC_2801|Compound:7907800|FD-Score:4.75|P-value:3.02E-6 ID:SGTC_2803|Compound:7707263|FD-Score:4.18|P-value:3.39E-5 ID:SGTC_2837|Compound:9008476|FD-Score:5.47|P-value:9.25E-8 ID:SGTC_2978|Compound:9011784|FD-Score:-3.75|P-value:1.58E-4 ID:SGTC_3037|Compound:9091026|FD-Score:8.59|P-value:1.21E-16 ID:SGTC_3056|Compound:9094900|FD-Score:-3.28|P-value:8.20E-4 ID:SGTC_3087|Compound:9117406|FD-Score:3.32|P-value:7.91E-4 ID:SGTC_3124|Compound:9127567|FD-Score:-3.95|P-value:7.49E-5 ID:SGTC_3152|Compound:9099253|FD-Score:15.90|P-value:3.76E-52 ID:SGTC_3159|Compound:9100096|FD-Score:3.81|P-value:1.42E-4

Top fitness defect scores for YLR057W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3152 9099253 15.90 49.5 µM 3.76E-52 NEO1-PIK1 5 5.94E-57
2 SGTC_221 trifluoperazine 14.30 10.3 µM 6.10E-43 NEO1-PIK1 27 7.15E-47
3 SGTC_1781 st038725 10.90 66.3 µM 1.58E-25 NEO1 15 8.20E-28
4 SGTC_637 1181-0519 10.70 7.3 µM 8.96E-25 fatty acid desaturase (OLE1) 18 5.46E-27
5 SGTC_568 1193-0097 9.34 7.5 µM 2.33E-19 ERAD & cell cycle 28 4.61E-21
6 SGTC_384 k007-0144 8.60 5.2 µM 1.12E-16 ERAD & cell cycle 11 3.97E-18
7 SGTC_3037 9091026 8.59 49.5 µM 1.21E-16 NEO1-PIK1 10 4.33E-18
8 SGTC_1800 st050079 7.19 29.1 µM 3.53E-12 NEO1-PIK1 21 3.33E-13
9 SGTC_1713 st033146 6.94 12.8 µM 1.73E-11 2 1.90E-12
10 SGTC_232 clozapine 6.56 119.0 µM 1.96E-10 NEO1 27 2.70E-11
11 SGTC_1161 k007-0610 6.54 110.0 µM 2.21E-10 ERAD & cell cycle 48 3.07E-11
12 SGTC_2658 polymyxin b sulfate 6.18 77.0 µM 1.89E-9 NEO1 21 3.23E-10
13 SGTC_1803 st050068 6.08 52.4 µM 3.25E-9 NEO1-PIK1 82 5.83E-10
14 SGTC_15 0266-0086 5.89 27.1 µM 9.82E-9 endomembrane recycling 22 1.95E-9
15 SGTC_1991 st073000 5.73 8.9 µM 2.36E-8 NEO1 39 5.09E-9
16 SGTC_547 1120-0019 5.72 610.0 nM 2.49E-8 azole & statin 9 5.41E-9
17 SGTC_2345 9052315 5.65 157.4 µM 3.48E-8 5 7.80E-9
18 SGTC_2837 9008476 5.47 45.5 µM 9.25E-8 9 2.27E-8
19 SGTC_1818 st052136 5.37 56.3 µM 1.54E-7 NEO1-PIK1 18 3.96E-8
20 SGTC_1954 st077032 4.97 68.9 µM 1.10E-6 5 3.42E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.425 2.97E-147 YDR372C VPS74 hom Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
0.424 5.94E-147 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.418 5.69E-142 YDR456W NHX1 hom Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion
0.400 1.74E-129 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.399 1.28E-128 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.387 2.58E-120 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.379 3.93E-115 YBR246W RRT2 hom Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.378 4.06E-114 YIL048W NEO1 het Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
0.375 2.04E-112 YHR004C NEM1 hom Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology and sporulation; homolog of the human protein Dullard
0.373 5.63E-111 YAL009W SPO7 hom Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation
0.367 3.93E-107 YOR039W CKB2 hom Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
0.364 7.27E-106 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.363 7.37E-105 YDR455C_d YDR455C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
0.355 3.87E-100 YGL019W CKB1 hom Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
0.353 2.70E-99 YKL039W PTM1 hom Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication