YLR060W / FRS1

Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR060W / FRS1

Click on Significant Values for Screen Details ID:SGTC_55|Compound:0767-0285|FD-Score:3.24|P-value:2.54E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:4.53|P-value:5.75E-7 ID:SGTC_165|Compound:k038-0023|FD-Score:3.87|P-value:1.60E-5 ID:SGTC_167|Compound:3672-0051|FD-Score:4.24|P-value:2.55E-6 ID:SGTC_186|Compound:3455-3465|FD-Score:-3.78|P-value:2.13E-5 ID:SGTC_189|Compound:3970-0742|FD-Score:-3.38|P-value:1.27E-4 ID:SGTC_200|Compound:k038-0079|FD-Score:4.09|P-value:5.68E-6 ID:SGTC_205|Compound:1486-1330|FD-Score:3.05|P-value:5.24E-4 ID:SGTC_216|Compound:k038-0035|FD-Score:5.79|P-value:2.40E-10 ID:SGTC_543|Compound:3844-1095|FD-Score:-3.47|P-value:8.54E-5 ID:SGTC_583|Compound:1488-1091|FD-Score:2.98|P-value:7.02E-4 ID:SGTC_608|Compound:k048-0125|FD-Score:14.10|P-value:4.35E-52 ID:SGTC_680|Compound:0417-1641|FD-Score:-3.15|P-value:3.17E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:3.03|P-value:5.67E-4 ID:SGTC_712|Compound:r015-0009|FD-Score:23.90|P-value:7.44E-147 ID:SGTC_727|Compound:3032-0951|FD-Score:4.58|P-value:4.18E-7 ID:SGTC_813|Compound:k007-0158|FD-Score:4.09|P-value:5.69E-6 ID:SGTC_815|Compound:1582-0027|FD-Score:3.36|P-value:1.57E-4 ID:SGTC_823|Compound:0417-1665|FD-Score:-4.16|P-value:3.44E-6 ID:SGTC_855|Compound:0428-0020|FD-Score:2.96|P-value:7.39E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:4.50|P-value:6.53E-7 ID:SGTC_936|Compound:3005-4620|FD-Score:2.95|P-value:7.80E-4 ID:SGTC_945|Compound:3381-0768|FD-Score:2.93|P-value:8.32E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:3.15|P-value:3.63E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:12.40|P-value:6.46E-41 ID:SGTC_1291|Compound:0993-0025|FD-Score:3.45|P-value:1.06E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:5.32|P-value:5.18E-9 ID:SGTC_1640|Compound:st009078|FD-Score:-2.93|P-value:7.54E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-4.16|P-value:3.33E-6 ID:SGTC_1712|Compound:st032288|FD-Score:3.48|P-value:9.15E-5 ID:SGTC_1799|Compound:st045822|FD-Score:3.20|P-value:2.97E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.01|P-value:6.30E-4 ID:SGTC_2163|Compound:5671233|FD-Score:-3.63|P-value:4.16E-5 ID:SGTC_2331|Compound:7980721|FD-Score:4.34|P-value:1.54E-6 ID:SGTC_2339|Compound:9000433|FD-Score:-4.23|P-value:2.35E-6 ID:SGTC_2429|Compound:5902844|FD-Score:3.95|P-value:1.10E-5 ID:SGTC_2448|Compound:5185112|FD-Score:-4.18|P-value:3.11E-6 ID:SGTC_2451|Compound:5856223|FD-Score:3.74|P-value:2.91E-5 ID:SGTC_2453|Compound:5731132|FD-Score:4.01|P-value:8.11E-6 ID:SGTC_2857|Compound:9024691|FD-Score:-3.01|P-value:5.54E-4 ID:SGTC_3141|Compound:9094609|FD-Score:-3.12|P-value:3.67E-4 ID:SGTC_3284|Compound:9108171|FD-Score:3.16|P-value:3.50E-4 ID:SGTC_3352|Compound:9151559|FD-Score:-2.97|P-value:6.34E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:3.24|P-value:2.54E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:4.53|P-value:5.75E-7 ID:SGTC_165|Compound:k038-0023|FD-Score:3.87|P-value:1.60E-5 ID:SGTC_167|Compound:3672-0051|FD-Score:4.24|P-value:2.55E-6 ID:SGTC_186|Compound:3455-3465|FD-Score:-3.78|P-value:2.13E-5 ID:SGTC_189|Compound:3970-0742|FD-Score:-3.38|P-value:1.27E-4 ID:SGTC_200|Compound:k038-0079|FD-Score:4.09|P-value:5.68E-6 ID:SGTC_205|Compound:1486-1330|FD-Score:3.05|P-value:5.24E-4 ID:SGTC_216|Compound:k038-0035|FD-Score:5.79|P-value:2.40E-10 ID:SGTC_543|Compound:3844-1095|FD-Score:-3.47|P-value:8.54E-5 ID:SGTC_583|Compound:1488-1091|FD-Score:2.98|P-value:7.02E-4 ID:SGTC_608|Compound:k048-0125|FD-Score:14.10|P-value:4.35E-52 ID:SGTC_680|Compound:0417-1641|FD-Score:-3.15|P-value:3.17E-4 ID:SGTC_696|Compound:1560-0013|FD-Score:3.03|P-value:5.67E-4 ID:SGTC_712|Compound:r015-0009|FD-Score:23.90|P-value:7.44E-147 ID:SGTC_727|Compound:3032-0951|FD-Score:4.58|P-value:4.18E-7 ID:SGTC_813|Compound:k007-0158|FD-Score:4.09|P-value:5.69E-6 ID:SGTC_815|Compound:1582-0027|FD-Score:3.36|P-value:1.57E-4 ID:SGTC_823|Compound:0417-1665|FD-Score:-4.16|P-value:3.44E-6 ID:SGTC_855|Compound:0428-0020|FD-Score:2.96|P-value:7.39E-4 ID:SGTC_911|Compound:3456-4043|FD-Score:4.50|P-value:6.53E-7 ID:SGTC_936|Compound:3005-4620|FD-Score:2.95|P-value:7.80E-4 ID:SGTC_945|Compound:3381-0768|FD-Score:2.93|P-value:8.32E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:3.15|P-value:3.63E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:12.40|P-value:6.46E-41 ID:SGTC_1291|Compound:0993-0025|FD-Score:3.45|P-value:1.06E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:5.32|P-value:5.18E-9 ID:SGTC_1640|Compound:st009078|FD-Score:-2.93|P-value:7.54E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-4.16|P-value:3.33E-6 ID:SGTC_1712|Compound:st032288|FD-Score:3.48|P-value:9.15E-5 ID:SGTC_1799|Compound:st045822|FD-Score:3.20|P-value:2.97E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.01|P-value:6.30E-4 ID:SGTC_2163|Compound:5671233|FD-Score:-3.63|P-value:4.16E-5 ID:SGTC_2331|Compound:7980721|FD-Score:4.34|P-value:1.54E-6 ID:SGTC_2339|Compound:9000433|FD-Score:-4.23|P-value:2.35E-6 ID:SGTC_2429|Compound:5902844|FD-Score:3.95|P-value:1.10E-5 ID:SGTC_2448|Compound:5185112|FD-Score:-4.18|P-value:3.11E-6 ID:SGTC_2451|Compound:5856223|FD-Score:3.74|P-value:2.91E-5 ID:SGTC_2453|Compound:5731132|FD-Score:4.01|P-value:8.11E-6 ID:SGTC_2857|Compound:9024691|FD-Score:-3.01|P-value:5.54E-4 ID:SGTC_3141|Compound:9094609|FD-Score:-3.12|P-value:3.67E-4 ID:SGTC_3284|Compound:9108171|FD-Score:3.16|P-value:3.50E-4 ID:SGTC_3352|Compound:9151559|FD-Score:-2.97|P-value:6.34E-4

Top fitness defect scores for YLR060W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_712 r015-0009 23.90 80.6 µM 7.44E-147 1 8.57E-127 8.95
2 SGTC_608 k048-0125 14.10 159.0 µM 4.35E-52 1 3.49E-45 6.12
3 SGTC_1248 0469-0687 12.40 58.6 µM 6.46E-41 1 1.48E-35 3.59
4 SGTC_216 k038-0035 5.79 28.4 µM 2.40E-10 ubiquinone biosynthesis & proteasome 5 3.56E-9 0.29
5 SGTC_1371 2421-0008 5.32 32.7 µM 5.18E-9 mitochondrial processes 12 5.08E-8 0.07
6 SGTC_727 3032-0951 4.58 202.0 µM 4.18E-7 3 2.28E-6 1.28
7 SGTC_158 k038-0087 4.53 26.4 µM 5.75E-7 ubiquinone biosynthesis & proteasome 25 3.01E-6 0.01
8 SGTC_911 3456-4043 4.50 236.0 µM 6.53E-7 mitochondrial processes 6 3.36E-6 0.01
9 SGTC_2331 7980721 4.34 200.0 µM 1.54E-6 10 7.06E-6 0.06
10 SGTC_167 3672-0051 4.24 66.2 µM 2.55E-6 7 1.10E-5 0.22
11 SGTC_200 k038-0079 4.09 37.5 µM 5.68E-6 ubiquinone biosynthesis & proteasome 11 2.20E-5 0.18
12 SGTC_813 k007-0158 4.09 173.0 µM 5.69E-6 fatty acid desaturase (OLE1) 5 2.20E-5 0.27
13 SGTC_2453 5731132 4.01 22.2 µM 8.11E-6 4 3.00E-5 0.30
14 SGTC_2429 5902844 3.95 64.4 µM 1.10E-5 8 3.91E-5 0.02
15 SGTC_165 k038-0023 3.87 64.4 µM 1.60E-5 ubiquinone biosynthesis & proteasome 23 5.40E-5 0.19
16 SGTC_2451 5856223 3.74 45.7 µM 2.91E-5 12 9.10E-5 0.33
17 SGTC_1712 st032288 3.48 53.4 µM 9.15E-5 calcium & mitochondrial duress 44 2.47E-4 0.01
18 SGTC_1291 0993-0025 3.45 5.8 µM 1.06E-4 2 2.80E-4 0.14
19 SGTC_815 1582-0027 3.36 36.9 µM 1.57E-4 8 3.95E-4 0.01
20 SGTC_55 0767-0285 3.24 6.6 µM 2.54E-4 14 6.00E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.461 8.64E-176 YPL183C RTT10 hom WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; evidence it interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6
0.342 1.27E-92 YFL022C FRS2 het Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
0.248 4.32E-48 YBR061C TRM7 hom 2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of the tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively
0.160 1.15E-20 YGL196W DSD1 hom D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate
0.140 4.36E-16 YHR094C HXT1 hom Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication
0.135 4.89E-15 YPL126W NAN1 het U3 snoRNP protein, component of the small (ribosomal) subunit (SSU) processosome containing U3 snoRNA; required for the biogenesis of18S rRNA
0.122 1.56E-12 YDR210W_p YDR210W_p hom Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.121 1.85E-12 YPR079W MRL1 hom Membrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress
0.121 2.09E-12 YDR174W HMO1 hom Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase
0.120 3.34E-12 YER019C-A SBH2 hom Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p
0.114 3.61E-11 YLR082C SRL2 hom Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation
0.113 6.40E-11 YOR033C EXO1 hom 5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair and DNA mismatch repair; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress
0.112 6.64E-11 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.112 7.39E-11 YPR150W_d YPR150W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C
0.112 7.50E-11 YMR016C SOK2 hom Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; SOK2 has a paralog, PHD1, that arose from the whole genome duplication