YLR062C_d / BUD28

Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR062C / BUD28

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:3.40|P-value:7.80E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-3.67|P-value:2.70E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:4.08|P-value:7.30E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:3.67|P-value:3.18E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.35|P-value:7.89E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:3.82|P-value:1.87E-4 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:-3.34|P-value:8.16E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:-4.39|P-value:1.81E-5 ID:SGTC_398|Compound:bronopol|FD-Score:-3.39|P-value:7.06E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.32|P-value:8.78E-4 ID:SGTC_441|Compound:propafenone|FD-Score:4.20|P-value:4.66E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.53|P-value:4.34E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.81|P-value:3.66E-6 ID:SGTC_1057|Compound:3448-5381|FD-Score:4.03|P-value:8.80E-5 ID:SGTC_1092|Compound:toremifene|FD-Score:3.41|P-value:7.68E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:5.30|P-value:3.93E-7 ID:SGTC_1228|Compound:0124-0032|FD-Score:5.84|P-value:2.57E-8 ID:SGTC_1407|Compound:3909-7909|FD-Score:3.69|P-value:3.01E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-5.20|P-value:5.05E-7 ID:SGTC_1781|Compound:st038725|FD-Score:4.16|P-value:5.37E-5 ID:SGTC_2003|Compound:4023581|FD-Score:3.45|P-value:6.67E-4 ID:SGTC_2059|Compound:5238311|FD-Score:3.85|P-value:1.68E-4 ID:SGTC_2164|Compound:5685496|FD-Score:-3.41|P-value:6.53E-4 ID:SGTC_2287|Compound:7945887|FD-Score:3.56|P-value:4.68E-4 ID:SGTC_2300|Compound:7976697|FD-Score:3.65|P-value:3.36E-4 ID:SGTC_2484|Compound:5809818|FD-Score:3.42|P-value:7.33E-4 ID:SGTC_2551|Compound:apiole|FD-Score:-3.86|P-value:1.38E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.07|P-value:7.43E-5 ID:SGTC_2809|Compound:7964133|FD-Score:3.92|P-value:1.30E-4 ID:SGTC_2990|Compound:9074105|FD-Score:4.54|P-value:1.17E-5 ID:SGTC_2992|Compound:9074591|FD-Score:3.81|P-value:1.97E-4 ID:SGTC_3066|Compound:9117245|FD-Score:3.35|P-value:9.08E-4 ID:SGTC_37|Compound:0566-0070|FD-Score:3.40|P-value:7.80E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-3.67|P-value:2.70E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:4.08|P-value:7.30E-5 ID:SGTC_118|Compound:0083-0097|FD-Score:3.67|P-value:3.18E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.35|P-value:7.89E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:3.82|P-value:1.87E-4 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:-3.34|P-value:8.16E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:-4.39|P-value:1.81E-5 ID:SGTC_398|Compound:bronopol|FD-Score:-3.39|P-value:7.06E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.32|P-value:8.78E-4 ID:SGTC_441|Compound:propafenone|FD-Score:4.20|P-value:4.66E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-3.53|P-value:4.34E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.81|P-value:3.66E-6 ID:SGTC_1057|Compound:3448-5381|FD-Score:4.03|P-value:8.80E-5 ID:SGTC_1092|Compound:toremifene|FD-Score:3.41|P-value:7.68E-4 ID:SGTC_1108|Compound:betulinic acid|FD-Score:5.30|P-value:3.93E-7 ID:SGTC_1228|Compound:0124-0032|FD-Score:5.84|P-value:2.57E-8 ID:SGTC_1407|Compound:3909-7909|FD-Score:3.69|P-value:3.01E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-5.20|P-value:5.05E-7 ID:SGTC_1781|Compound:st038725|FD-Score:4.16|P-value:5.37E-5 ID:SGTC_2003|Compound:4023581|FD-Score:3.45|P-value:6.67E-4 ID:SGTC_2059|Compound:5238311|FD-Score:3.85|P-value:1.68E-4 ID:SGTC_2164|Compound:5685496|FD-Score:-3.41|P-value:6.53E-4 ID:SGTC_2287|Compound:7945887|FD-Score:3.56|P-value:4.68E-4 ID:SGTC_2300|Compound:7976697|FD-Score:3.65|P-value:3.36E-4 ID:SGTC_2484|Compound:5809818|FD-Score:3.42|P-value:7.33E-4 ID:SGTC_2551|Compound:apiole|FD-Score:-3.86|P-value:1.38E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.07|P-value:7.43E-5 ID:SGTC_2809|Compound:7964133|FD-Score:3.92|P-value:1.30E-4 ID:SGTC_2990|Compound:9074105|FD-Score:4.54|P-value:1.17E-5 ID:SGTC_2992|Compound:9074591|FD-Score:3.81|P-value:1.97E-4 ID:SGTC_3066|Compound:9117245|FD-Score:3.35|P-value:9.08E-4

Top fitness defect scores for YLR062C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1228 0124-0032 5.84 52.5 µM 2.57E-8 1 2.62E-9
2 SGTC_1108 betulinic acid 5.30 729.1 nM 3.93E-7 4 5.87E-8
3 SGTC_800 3448-7362 4.81 155.0 µM 3.66E-6 translation 99 7.48E-7
4 SGTC_2990 9074105 4.54 71.4 µM 1.17E-5 2 2.82E-6
5 SGTC_441 propafenone 4.20 146.0 µM 4.66E-5 4 1.36E-5
6 SGTC_1781 st038725 4.16 66.3 µM 5.37E-5 NEO1 92 1.59E-5
7 SGTC_97 0535-0701 4.08 19.1 µM 7.30E-5 60S ribosome export 13 2.26E-5
8 SGTC_2688 kanamycin b 4.07 96.7 µM 7.43E-5 calcium & mitochondrial duress 351 2.31E-5
9 SGTC_1057 3448-5381 4.03 134.0 µM 8.80E-5 calcium & mitochondrial duress 24 2.80E-5
10 SGTC_2809 7964133 3.92 13.0 µM 1.30E-4 60S ribosome export 41 4.37E-5
11 SGTC_2059 5238311 3.85 157.0 µM 1.68E-4 29 5.85E-5
12 SGTC_140 0062-0056 3.82 502.5 µM 1.87E-4 26 6.62E-5
13 SGTC_2992 9074591 3.81 71.4 µM 1.97E-4 17 7.02E-5
14 SGTC_1407 3909-7909 3.69 12.3 µM 3.01E-4 60S ribosome export 19 1.14E-4
15 SGTC_118 0083-0097 3.67 135.3 µM 3.18E-4 translation 165 1.21E-4
16 SGTC_2300 7976697 3.65 71.6 µM 3.36E-4 ERG2 40 1.29E-4
17 SGTC_2287 7945887 3.56 168.8 µM 4.68E-4 13 1.87E-4
18 SGTC_2003 4023581 3.45 200.0 µM 6.67E-4 cell wall signaling 50 2.81E-4
19 SGTC_2484 5809818 3.42 12.0 µM 7.33E-4 29 3.13E-4
20 SGTC_1092 toremifene 3.41 6.3 µM 7.68E-4 38 3.30E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.167 2.31E-22 YLR061W RPL22A hom Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
0.164 1.38E-21 YBR267W REI1 hom Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
0.153 5.04E-19 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.150 2.84E-18 YJL189W RPL39 hom Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog
0.148 8.11E-18 YJL136C RPS21B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication
0.141 2.22E-16 YJR145C RPS4A hom Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication
0.141 2.35E-16 YDR161W_p YDR161W_p hom Putative protein of unknown function; non-essential gene; proposed function in rRNA and ribosome biosynthesis based on transcriptional co-regulation; genetic interactions suggest a role in ER-associated protein degradation (ERAD)
0.139 4.99E-16 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.132 1.43E-14 YJL188C_d BUD19_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.129 5.78E-14 YDL061C RPS29B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication
0.128 1.22E-13 YBR189W RPS9B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication
0.125 3.44E-13 YDR101C ARX1 hom Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex
0.124 4.95E-13 YLR185W RPL37A hom Ribosomal 60S subunit protein L37A; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication
0.123 8.02E-13 YDL082W RPL13A hom Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication
0.123 8.20E-13 YDR083W RRP8 hom Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1