YLR071C / RGR1

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR071C / RGR1

Click on Significant Values for Screen Details ID:SGTC_127|Compound:0180-0333|FD-Score:3.18|P-value:4.18E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:3.16|P-value:4.47E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:-3.19|P-value:4.10E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:3.03|P-value:7.27E-4 ID:SGTC_444|Compound:curcumin|FD-Score:3.34|P-value:2.27E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.67|P-value:4.51E-7 ID:SGTC_568|Compound:1193-0097|FD-Score:3.48|P-value:1.28E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:3.24|P-value:3.35E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:3.09|P-value:5.79E-4 ID:SGTC_1088|Compound:bifonazole|FD-Score:2.97|P-value:9.04E-4 ID:SGTC_1199|Compound:1638-0010|FD-Score:-3.48|P-value:1.28E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.12|P-value:7.54E-6 ID:SGTC_1257|Compound:0669-0121|FD-Score:-4.66|P-value:4.89E-7 ID:SGTC_1260|Compound:0700-1617|FD-Score:4.19|P-value:5.45E-6 ID:SGTC_1369|Compound:1866-0067|FD-Score:3.15|P-value:4.59E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.18|P-value:4.22E-4 ID:SGTC_1661|Compound:st013063|FD-Score:-2.98|P-value:8.91E-4 ID:SGTC_1834|Compound:st055981|FD-Score:3.19|P-value:4.04E-4 ID:SGTC_1840|Compound:st055363|FD-Score:-3.01|P-value:7.94E-4 ID:SGTC_1910|Compound:st062352|FD-Score:2.97|P-value:9.13E-4 ID:SGTC_1950|Compound:st077106|FD-Score:-3.52|P-value:1.08E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:-3.60|P-value:7.73E-5 ID:SGTC_2052|Compound:5265182|FD-Score:5.31|P-value:1.24E-8 ID:SGTC_2133|Compound:5320837|FD-Score:-4.80|P-value:2.28E-7 ID:SGTC_2785|Compound:7756463|FD-Score:3.95|P-value:1.63E-5 ID:SGTC_2896|Compound:9052656|FD-Score:3.01|P-value:7.74E-4 ID:SGTC_2935|Compound:9038016|FD-Score:3.18|P-value:4.21E-4 ID:SGTC_2944|Compound:9067346|FD-Score:4.53|P-value:9.95E-7 ID:SGTC_2948|Compound:9070189|FD-Score:4.87|P-value:1.51E-7 ID:SGTC_2965|Compound:9082772|FD-Score:-4.77|P-value:2.72E-7 ID:SGTC_127|Compound:0180-0333|FD-Score:3.18|P-value:4.18E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:3.16|P-value:4.47E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:-3.19|P-value:4.10E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:3.03|P-value:7.27E-4 ID:SGTC_444|Compound:curcumin|FD-Score:3.34|P-value:2.27E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:4.67|P-value:4.51E-7 ID:SGTC_568|Compound:1193-0097|FD-Score:3.48|P-value:1.28E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:3.24|P-value:3.35E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:3.09|P-value:5.79E-4 ID:SGTC_1088|Compound:bifonazole|FD-Score:2.97|P-value:9.04E-4 ID:SGTC_1199|Compound:1638-0010|FD-Score:-3.48|P-value:1.28E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.12|P-value:7.54E-6 ID:SGTC_1257|Compound:0669-0121|FD-Score:-4.66|P-value:4.89E-7 ID:SGTC_1260|Compound:0700-1617|FD-Score:4.19|P-value:5.45E-6 ID:SGTC_1369|Compound:1866-0067|FD-Score:3.15|P-value:4.59E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.18|P-value:4.22E-4 ID:SGTC_1661|Compound:st013063|FD-Score:-2.98|P-value:8.91E-4 ID:SGTC_1834|Compound:st055981|FD-Score:3.19|P-value:4.04E-4 ID:SGTC_1840|Compound:st055363|FD-Score:-3.01|P-value:7.94E-4 ID:SGTC_1910|Compound:st062352|FD-Score:2.97|P-value:9.13E-4 ID:SGTC_1950|Compound:st077106|FD-Score:-3.52|P-value:1.08E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:-3.60|P-value:7.73E-5 ID:SGTC_2052|Compound:5265182|FD-Score:5.31|P-value:1.24E-8 ID:SGTC_2133|Compound:5320837|FD-Score:-4.80|P-value:2.28E-7 ID:SGTC_2785|Compound:7756463|FD-Score:3.95|P-value:1.63E-5 ID:SGTC_2896|Compound:9052656|FD-Score:3.01|P-value:7.74E-4 ID:SGTC_2935|Compound:9038016|FD-Score:3.18|P-value:4.21E-4 ID:SGTC_2944|Compound:9067346|FD-Score:4.53|P-value:9.95E-7 ID:SGTC_2948|Compound:9070189|FD-Score:4.87|P-value:1.51E-7 ID:SGTC_2965|Compound:9082772|FD-Score:-4.77|P-value:2.72E-7

Top fitness defect scores for YLR071C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2052 5265182 5.31 7.1 µM 1.24E-8 2 5.59E-8 0.50
2 SGTC_2948 9070189 4.87 9.4 µM 1.51E-7 plasma membrane duress 6 5.44E-7 0.06
3 SGTC_453 enantio-paf c16 4.67 10.0 µM 4.51E-7 16 1.47E-6 0.14
4 SGTC_2944 9067346 4.53 25.6 µM 9.95E-7 8 3.02E-6 0.27
5 SGTC_1260 0700-1617 4.19 7.8 µM 5.45E-6 calcium & mitochondrial duress 6 1.42E-5 0.09
6 SGTC_1256 0570-0116 4.12 285.0 µM 7.54E-6 5 1.90E-5 0.61
7 SGTC_2785 7756463 3.95 72.7 µM 1.63E-5 19 3.84E-5 0.37
8 SGTC_568 1193-0097 3.48 7.5 µM 1.28E-4 ERAD & cell cycle 30 2.51E-4 0.02
9 SGTC_444 curcumin 3.34 90.4 µM 2.27E-4 iron homeostasis 25 4.23E-4 0.00
10 SGTC_969 1313-0238 3.24 134.0 µM 3.35E-4 mitochondrial processes 34 6.04E-4 0.02
11 SGTC_1834 st055981 3.19 52.8 µM 4.04E-4 RPP1 & pyrimidine depletion 21 7.17E-4 0.01
12 SGTC_127 0180-0333 3.18 59.1 µM 4.18E-4 mitochondrial response to ROS 3 7.38E-4 0.06
13 SGTC_2935 9038016 3.18 30.0 µM 4.21E-4 14 7.44E-4 0.00
14 SGTC_1371 2421-0008 3.18 32.7 µM 4.22E-4 mitochondrial processes 36 7.45E-4 0.02
15 SGTC_227 fluconazole 3.16 33.5 µM 4.47E-4 azole & statin 59 7.85E-4 0.05
16 SGTC_1369 1866-0067 3.15 208.0 µM 4.59E-4 RPP1 & pyrimidine depletion 14 8.04E-4 0.01
17 SGTC_1079 idarubicin 3.09 2.4 µM 5.79E-4 DNA damage response 13 9.94E-4 0.05
18 SGTC_431 k089-0097 3.03 107.0 µM 7.27E-4 iron homeostasis 26 0.00122 0.01
19 SGTC_2896 9052656 3.01 71.4 µM 7.74E-4 endomembrane recycling 32 0.00130 0.01
20 SGTC_1088 bifonazole 2.97 369.0 nM 9.04E-4 9 0.00149 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.149 4.18E-18 YOR174W MED4 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.147 9.12E-18 YML101C-A_d YML101C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.136 2.25E-15 YBL005W PDR3 hom Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.131 2.37E-14 YDL220C CDC13 het Single stranded DNA-binding protein found at TG1-3 telomere G-tails; regulates telomere replication through recruitment of specific sub-complexes, but the essential function is telomere capping; autophagy and proteasome are involved in Cdc13p degradation
0.118 5.67E-12 YDR478W SNM1 het Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
0.118 6.54E-12 YEL068C_p YEL068C_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.117 8.74E-12 YMR214W SCJ1 hom One of several homologs of bacterial chaperone DnaJ, located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins
0.117 9.09E-12 YLR262C-A TMA7 hom Protein of unknown that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress
0.114 3.08E-11 YBR092C PHO3 hom Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin
0.108 2.97E-10 YPR168W NUT2 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress
0.106 8.45E-10 YJL195C_d YJL195C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
0.103 2.29E-9 YOR056C NOB1 het Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress
0.103 2.63E-9 YLR310C CDC25 het Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
0.102 3.58E-9 YOL135C MED7 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.101 4.76E-9 YJL098W SAP185 hom Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p; SAP185 has a paralog, SAP190, that arose from the whole genome duplication