YLR073C / RFU1

Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR073C / RFU1

Click on Significant Values for Screen Details ID:SGTC_41|Compound:0478-0632|FD-Score:-3.72|P-value:2.15E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:-4.11|P-value:5.07E-5 ID:SGTC_139|Compound:0099-0334|FD-Score:3.44|P-value:6.50E-4 ID:SGTC_229|Compound:benomyl|FD-Score:3.68|P-value:2.89E-4 ID:SGTC_264|Compound:promethazine|FD-Score:3.99|P-value:9.60E-5 ID:SGTC_327|Compound:1273-0057|FD-Score:5.14|P-value:7.45E-7 ID:SGTC_340|Compound:k038-0059|FD-Score:4.46|P-value:1.48E-5 ID:SGTC_421|Compound:streptovitacin|FD-Score:-4.62|P-value:6.35E-6 ID:SGTC_424|Compound:splitomicin|FD-Score:4.15|P-value:5.12E-5 ID:SGTC_426|Compound:0988-0037|FD-Score:-3.50|P-value:4.54E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.62|P-value:3.11E-4 ID:SGTC_463|Compound:ikarugamyin|FD-Score:3.54|P-value:4.63E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.48|P-value:5.75E-4 ID:SGTC_486|Compound:niguldipine|FD-Score:4.98|P-value:1.53E-6 ID:SGTC_530|Compound:4358-1714|FD-Score:-4.08|P-value:5.66E-5 ID:SGTC_671|Compound:0560-0003|FD-Score:3.79|P-value:1.96E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:3.89|P-value:1.39E-4 ID:SGTC_684|Compound:1140-0066|FD-Score:-5.21|P-value:4.40E-7 ID:SGTC_746|Compound:1095-0025|FD-Score:3.84|P-value:1.68E-4 ID:SGTC_754|Compound:3474-0146|FD-Score:3.65|P-value:3.19E-4 ID:SGTC_1262|Compound:0737-0114|FD-Score:3.46|P-value:6.24E-4 ID:SGTC_1354|Compound:1498-1681|FD-Score:3.79|P-value:2.00E-4 ID:SGTC_1355|Compound:1499-0035|FD-Score:4.67|P-value:6.23E-6 ID:SGTC_1450|Compound:4112-4260|FD-Score:-3.92|P-value:1.04E-4 ID:SGTC_1665|Compound:st014639|FD-Score:3.99|P-value:9.45E-5 ID:SGTC_1745|Compound:st081495|FD-Score:3.55|P-value:4.56E-4 ID:SGTC_1768|Compound:st044301|FD-Score:4.27|P-value:3.24E-5 ID:SGTC_1779|Compound:st039427|FD-Score:3.99|P-value:9.47E-5 ID:SGTC_1955|Compound:wogonin|FD-Score:-4.75|P-value:3.55E-6 ID:SGTC_2011|Compound:4012611|FD-Score:-3.54|P-value:3.98E-4 ID:SGTC_2090|Compound:5301565|FD-Score:3.52|P-value:4.97E-4 ID:SGTC_2270|Compound:7924575|FD-Score:4.69|P-value:5.60E-6 ID:SGTC_2271|Compound:7849410|FD-Score:3.47|P-value:5.94E-4 ID:SGTC_2292|Compound:7671323|FD-Score:3.50|P-value:5.45E-4 ID:SGTC_2294|Compound:7971431|FD-Score:-3.96|P-value:8.83E-5 ID:SGTC_2437|Compound:5741531|FD-Score:3.54|P-value:4.67E-4 ID:SGTC_2587|Compound:piperic acid|FD-Score:4.60|P-value:8.28E-6 ID:SGTC_2682|Compound:pyrogallin|FD-Score:3.43|P-value:6.79E-4 ID:SGTC_2933|Compound:9026794|FD-Score:-3.82|P-value:1.51E-4 ID:SGTC_2997|Compound:9067075|FD-Score:4.51|P-value:1.22E-5 ID:SGTC_3211|Compound:9128781|FD-Score:3.61|P-value:3.70E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:-3.72|P-value:2.15E-4 ID:SGTC_130|Compound:0136-0219|FD-Score:-4.11|P-value:5.07E-5 ID:SGTC_139|Compound:0099-0334|FD-Score:3.44|P-value:6.50E-4 ID:SGTC_229|Compound:benomyl|FD-Score:3.68|P-value:2.89E-4 ID:SGTC_264|Compound:promethazine|FD-Score:3.99|P-value:9.60E-5 ID:SGTC_327|Compound:1273-0057|FD-Score:5.14|P-value:7.45E-7 ID:SGTC_340|Compound:k038-0059|FD-Score:4.46|P-value:1.48E-5 ID:SGTC_421|Compound:streptovitacin|FD-Score:-4.62|P-value:6.35E-6 ID:SGTC_424|Compound:splitomicin|FD-Score:4.15|P-value:5.12E-5 ID:SGTC_426|Compound:0988-0037|FD-Score:-3.50|P-value:4.54E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-3.62|P-value:3.11E-4 ID:SGTC_463|Compound:ikarugamyin|FD-Score:3.54|P-value:4.63E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.48|P-value:5.75E-4 ID:SGTC_486|Compound:niguldipine|FD-Score:4.98|P-value:1.53E-6 ID:SGTC_530|Compound:4358-1714|FD-Score:-4.08|P-value:5.66E-5 ID:SGTC_671|Compound:0560-0003|FD-Score:3.79|P-value:1.96E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:3.89|P-value:1.39E-4 ID:SGTC_684|Compound:1140-0066|FD-Score:-5.21|P-value:4.40E-7 ID:SGTC_746|Compound:1095-0025|FD-Score:3.84|P-value:1.68E-4 ID:SGTC_754|Compound:3474-0146|FD-Score:3.65|P-value:3.19E-4 ID:SGTC_1262|Compound:0737-0114|FD-Score:3.46|P-value:6.24E-4 ID:SGTC_1354|Compound:1498-1681|FD-Score:3.79|P-value:2.00E-4 ID:SGTC_1355|Compound:1499-0035|FD-Score:4.67|P-value:6.23E-6 ID:SGTC_1450|Compound:4112-4260|FD-Score:-3.92|P-value:1.04E-4 ID:SGTC_1665|Compound:st014639|FD-Score:3.99|P-value:9.45E-5 ID:SGTC_1745|Compound:st081495|FD-Score:3.55|P-value:4.56E-4 ID:SGTC_1768|Compound:st044301|FD-Score:4.27|P-value:3.24E-5 ID:SGTC_1779|Compound:st039427|FD-Score:3.99|P-value:9.47E-5 ID:SGTC_1955|Compound:wogonin|FD-Score:-4.75|P-value:3.55E-6 ID:SGTC_2011|Compound:4012611|FD-Score:-3.54|P-value:3.98E-4 ID:SGTC_2090|Compound:5301565|FD-Score:3.52|P-value:4.97E-4 ID:SGTC_2270|Compound:7924575|FD-Score:4.69|P-value:5.60E-6 ID:SGTC_2271|Compound:7849410|FD-Score:3.47|P-value:5.94E-4 ID:SGTC_2292|Compound:7671323|FD-Score:3.50|P-value:5.45E-4 ID:SGTC_2294|Compound:7971431|FD-Score:-3.96|P-value:8.83E-5 ID:SGTC_2437|Compound:5741531|FD-Score:3.54|P-value:4.67E-4 ID:SGTC_2587|Compound:piperic acid|FD-Score:4.60|P-value:8.28E-6 ID:SGTC_2682|Compound:pyrogallin|FD-Score:3.43|P-value:6.79E-4 ID:SGTC_2933|Compound:9026794|FD-Score:-3.82|P-value:1.51E-4 ID:SGTC_2997|Compound:9067075|FD-Score:4.51|P-value:1.22E-5 ID:SGTC_3211|Compound:9128781|FD-Score:3.61|P-value:3.70E-4

Top fitness defect scores for YLR073C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_327 1273-0057 5.14 4.0 µM 7.45E-7 azole & statin 10 1.37E-7
2 SGTC_486 niguldipine 4.98 82.0 µM 1.53E-6 amide catabolism 8 3.10E-7
3 SGTC_2270 7924575 4.69 90.9 µM 5.60E-6 RPP1 & pyrimidine depletion 7 1.34E-6
4 SGTC_1355 1499-0035 4.67 1.7 µM 6.23E-6 5 1.51E-6
5 SGTC_2587 piperic acid 4.60 92.2 µM 8.28E-6 10 2.08E-6
6 SGTC_2997 9067075 4.51 71.4 µM 1.22E-5 unfolded protein response 39 3.22E-6
7 SGTC_340 k038-0059 4.46 454.0 µM 1.48E-5 ubiquinone biosynthesis & proteasome 39 4.02E-6
8 SGTC_1768 st044301 4.27 37.2 µM 3.24E-5 12 9.75E-6
9 SGTC_424 splitomicin 4.15 100.0 mM 5.12E-5 17 1.63E-5
10 SGTC_1665 st014639 3.99 11.4 µM 9.45E-5 NEO1 21 3.26E-5
11 SGTC_1779 st039427 3.99 55.0 µM 9.47E-5 27 3.27E-5
12 SGTC_264 promethazine 3.99 12.5 mM 9.60E-5 DNA intercalators 52 3.32E-5
13 SGTC_676 falcarinol 3.89 140.0 µM 1.39E-4 57 5.05E-5
14 SGTC_746 1095-0025 3.84 46.6 µM 1.68E-4 RSC & ERG11 17 6.24E-5
15 SGTC_671 0560-0003 3.79 407.0 µM 1.96E-4 14 7.45E-5
16 SGTC_1354 1498-1681 3.79 81.0 µM 2.00E-4 25 7.62E-5
17 SGTC_229 benomyl 3.68 22.9 µM 2.89E-4 tubulin folding & SWR complex 37 1.15E-4
18 SGTC_754 3474-0146 3.65 171.0 µM 3.19E-4 copper-dependent oxidative stress 34 1.29E-4
19 SGTC_3211 9128781 3.61 49.5 µM 3.70E-4 82 1.52E-4
20 SGTC_1745 st081495 3.55 74.0 µM 4.56E-4 RNA processing & uracil transport 88 1.93E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.088 3.67E-7 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.083 1.32E-6 YER057C HMF1 hom Member of the p14.5 protein family with similarity to Mmf1p, functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro
0.079 4.41E-6 YML041C VPS71 hom Nucleosome-binding component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
0.079 4.52E-6 YDR469W SDC1 hom Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30
0.076 1.06E-5 YGR023W MTL1 hom Putative plasma membrane sensor, involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; has structural and functional similarity to Mid2p
0.075 1.44E-5 YKL191W DPH2 hom Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p
0.073 2.12E-5 YIL061C SNP1 het Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of the arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1 70K protein; protein abundance increases in response to DNA replication stress
0.073 2.51E-5 YOR250C CLP1 het Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; involved in both the endonucleolyitc cleavage and polyadenylation steps of mRNA 3'-end maturation
0.072 3.23E-5 YKL112W ABF1 het DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair
0.071 3.54E-5 YMR006C PLB2 hom Phospholipase B (lysophospholipase) involved in phospholipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine
0.071 3.67E-5 YJL202C_d YJL202C_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex
0.071 3.68E-5 YLR106C MDN1 het Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus
0.070 4.90E-5 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.070 5.21E-5 YLR241W_p YLR241W_p hom Putative protein of unknown function, may be involved in detoxification
0.070 5.31E-5 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain