YLR077W / FMP25

Mitochondrial inner membrane protein required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR077W / FMP25

Click on Significant Values for Screen Details ID:SGTC_61|Compound:0978-8327|FD-Score:-3.89|P-value:5.57E-5 ID:SGTC_62|Compound:0180-0423|FD-Score:-3.21|P-value:7.03E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:6.56|P-value:4.58E-11 ID:SGTC_362|Compound:0310-0100|FD-Score:4.65|P-value:2.24E-6 ID:SGTC_480|Compound:am 92016|FD-Score:4.24|P-value:1.40E-5 ID:SGTC_620|Compound:0109-0052|FD-Score:-3.14|P-value:8.82E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:4.28|P-value:1.18E-5 ID:SGTC_629|Compound:3448-6466|FD-Score:-3.19|P-value:7.59E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.65|P-value:1.59E-4 ID:SGTC_722|Compound:3534-0461|FD-Score:4.21|P-value:1.63E-5 ID:SGTC_725|Compound:1497-0963|FD-Score:3.95|P-value:4.85E-5 ID:SGTC_740|Compound:k014-0057|FD-Score:3.88|P-value:6.57E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:3.70|P-value:1.32E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:5.19|P-value:1.51E-7 ID:SGTC_843|Compound:0310-0051|FD-Score:3.19|P-value:8.21E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:-4.89|P-value:5.82E-7 ID:SGTC_933|Compound:2914-0560|FD-Score:-3.58|P-value:1.87E-4 ID:SGTC_1175|Compound:0741-0056|FD-Score:4.19|P-value:1.80E-5 ID:SGTC_1286|Compound:0929-0070|FD-Score:-4.86|P-value:6.97E-7 ID:SGTC_1396|Compound:3511-0007|FD-Score:4.62|P-value:2.55E-6 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.92|P-value:5.57E-5 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.50|P-value:2.79E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.32|P-value:5.27E-4 ID:SGTC_1939|Compound:st074455|FD-Score:8.27|P-value:1.49E-16 ID:SGTC_1953|Compound:st076800|FD-Score:4.82|P-value:9.56E-7 ID:SGTC_2150|Compound:5556613|FD-Score:-3.22|P-value:6.71E-4 ID:SGTC_2255|Compound:7941087|FD-Score:3.22|P-value:7.40E-4 ID:SGTC_2411|Compound:st077133|FD-Score:3.24|P-value:6.96E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:-3.54|P-value:2.11E-4 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:5.38|P-value:5.36E-8 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-3.44|P-value:3.08E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:5.89|P-value:2.90E-9 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.79|P-value:9.15E-5 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:4.16|P-value:1.99E-5 ID:SGTC_2936|Compound:9038235|FD-Score:3.72|P-value:1.21E-4 ID:SGTC_2974|Compound:9094440|FD-Score:3.51|P-value:2.69E-4 ID:SGTC_3005|Compound:9076496|FD-Score:3.62|P-value:1.76E-4 ID:SGTC_3110|Compound:9122132|FD-Score:3.33|P-value:5.17E-4 ID:SGTC_3119|Compound:9125183|FD-Score:-4.35|P-value:7.80E-6 ID:SGTC_61|Compound:0978-8327|FD-Score:-3.89|P-value:5.57E-5 ID:SGTC_62|Compound:0180-0423|FD-Score:-3.21|P-value:7.03E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:6.56|P-value:4.58E-11 ID:SGTC_362|Compound:0310-0100|FD-Score:4.65|P-value:2.24E-6 ID:SGTC_480|Compound:am 92016|FD-Score:4.24|P-value:1.40E-5 ID:SGTC_620|Compound:0109-0052|FD-Score:-3.14|P-value:8.82E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:4.28|P-value:1.18E-5 ID:SGTC_629|Compound:3448-6466|FD-Score:-3.19|P-value:7.59E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.65|P-value:1.59E-4 ID:SGTC_722|Compound:3534-0461|FD-Score:4.21|P-value:1.63E-5 ID:SGTC_725|Compound:1497-0963|FD-Score:3.95|P-value:4.85E-5 ID:SGTC_740|Compound:k014-0057|FD-Score:3.88|P-value:6.57E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:3.70|P-value:1.32E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:5.19|P-value:1.51E-7 ID:SGTC_843|Compound:0310-0051|FD-Score:3.19|P-value:8.21E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:-4.89|P-value:5.82E-7 ID:SGTC_933|Compound:2914-0560|FD-Score:-3.58|P-value:1.87E-4 ID:SGTC_1175|Compound:0741-0056|FD-Score:4.19|P-value:1.80E-5 ID:SGTC_1286|Compound:0929-0070|FD-Score:-4.86|P-value:6.97E-7 ID:SGTC_1396|Compound:3511-0007|FD-Score:4.62|P-value:2.55E-6 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.92|P-value:5.57E-5 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:3.50|P-value:2.79E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.32|P-value:5.27E-4 ID:SGTC_1939|Compound:st074455|FD-Score:8.27|P-value:1.49E-16 ID:SGTC_1953|Compound:st076800|FD-Score:4.82|P-value:9.56E-7 ID:SGTC_2150|Compound:5556613|FD-Score:-3.22|P-value:6.71E-4 ID:SGTC_2255|Compound:7941087|FD-Score:3.22|P-value:7.40E-4 ID:SGTC_2411|Compound:st077133|FD-Score:3.24|P-value:6.96E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:-3.54|P-value:2.11E-4 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:5.38|P-value:5.36E-8 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-3.44|P-value:3.08E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:5.89|P-value:2.90E-9 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.79|P-value:9.15E-5 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:4.16|P-value:1.99E-5 ID:SGTC_2936|Compound:9038235|FD-Score:3.72|P-value:1.21E-4 ID:SGTC_2974|Compound:9094440|FD-Score:3.51|P-value:2.69E-4 ID:SGTC_3005|Compound:9076496|FD-Score:3.62|P-value:1.76E-4 ID:SGTC_3110|Compound:9122132|FD-Score:3.33|P-value:5.17E-4 ID:SGTC_3119|Compound:9125183|FD-Score:-4.35|P-value:7.80E-6

Top fitness defect scores for YLR077W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1939 st074455 8.27 53.6 µM 1.49E-16 1 6.85E-17
2 SGTC_236 1509-0011 6.56 5.3 µM 4.58E-11 272 2.74E-11
3 SGTC_2563 tomatine 5.89 8.5 µM 2.90E-9 9 1.90E-9
4 SGTC_2535 4',4'-dimethoxydalbergione 5.38 51.2 µM 5.36E-8 DNA damage response 6 3.73E-8
5 SGTC_840 0245-0196 5.19 390.0 µM 1.51E-7 3 1.08E-7
6 SGTC_1953 st076800 4.82 12.1 µM 9.56E-7 29 7.06E-7
7 SGTC_362 0310-0100 4.65 9.0 µM 2.24E-6 RPP1 & pyrimidine depletion 18 1.69E-6
8 SGTC_1396 3511-0007 4.62 98.6 µM 2.55E-6 mitochondrial processes 7 1.93E-6
9 SGTC_627 1391-0720 4.28 175.0 µM 1.18E-5 8 9.17E-6
10 SGTC_480 am 92016 4.24 50.0 µM 1.40E-5 7 1.10E-5
11 SGTC_722 3534-0461 4.21 190.0 µM 1.63E-5 PDR1 28 1.28E-5
12 SGTC_1175 0741-0056 4.19 27.6 µM 1.80E-5 11 1.42E-5
13 SGTC_2599 2',3-dihydroxy-4,4',6'-trimethoxychalcone 4.16 100.0 µM 1.99E-5 30 1.57E-5
14 SGTC_725 1497-0963 3.95 19.0 µM 4.85E-5 copper-dependent oxidative stress 18 3.90E-5
15 SGTC_1484 3473-2379 3.92 286.0 µM 5.57E-5 15 4.50E-5
16 SGTC_740 k014-0057 3.88 261.0 µM 6.57E-5 28 5.31E-5
17 SGTC_2564 betulinic acid 3.79 100.0 µM 9.15E-5 35 7.46E-5
18 SGTC_2936 9038235 3.72 15.6 µM 1.21E-4 27 9.95E-5
19 SGTC_750 4073-0104 3.70 132.0 µM 1.32E-4 14 1.08E-4
20 SGTC_690 glyoxal 3.65 20.3 µM 1.59E-4 95 1.32E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.181 3.19E-26 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.175 2.20E-24 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.172 8.09E-24 YCL057C-A MOS1 hom Mitochondrial protein essential for proper inner membrane organization; conserved component of the mitochondrial inner membrane organizing system (MICOS, MINOS, or MitOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture; ortholog of human MINOS1
0.172 1.50E-23 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.169 8.80E-23 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding
0.163 1.75E-21 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.163 2.34E-21 YJL023C PET130 hom Protein required for respiratory growth; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.161 6.11E-21 YKL109W HAP4 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex
0.161 7.03E-21 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.160 1.10E-20 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.158 2.62E-20 YKL087C CYT2 hom Cytochrome c1 heme lyase, involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1
0.155 1.79E-19 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.153 4.25E-19 YDR518W EUG1 hom Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.151 1.75E-18 YER141W COX15 hom Protein required for the hydroxylation of heme O to form heme A, which is an essential prosthetic group for cytochrome c oxidase
0.148 5.62E-18 YOR065W CYT1 hom Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex