YLR082C / SRL2

Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR082C / SRL2

Click on Significant Values for Screen Details ID:SGTC_316|Compound:k060-0003|FD-Score:3.17|P-value:7.89E-4 ID:SGTC_340|Compound:k038-0059|FD-Score:3.26|P-value:5.80E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:-4.70|P-value:1.68E-6 ID:SGTC_817|Compound:1391-0715|FD-Score:3.21|P-value:6.88E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:3.48|P-value:2.62E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:3.79|P-value:8.15E-5 ID:SGTC_875|Compound:0709-0210|FD-Score:3.52|P-value:2.24E-4 ID:SGTC_945|Compound:3381-0768|FD-Score:4.44|P-value:4.96E-6 ID:SGTC_946|Compound:3434-1334|FD-Score:4.36|P-value:7.24E-6 ID:SGTC_958|Compound:1222-0007|FD-Score:3.40|P-value:3.51E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:3.21|P-value:6.89E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:8.19|P-value:1.91E-16 ID:SGTC_1385|Compound:3091-4834|FD-Score:-3.76|P-value:9.86E-5 ID:SGTC_1391|Compound:3442-0225|FD-Score:-3.36|P-value:4.42E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:3.51|P-value:2.34E-4 ID:SGTC_1675|Compound:st018485|FD-Score:-3.21|P-value:7.50E-4 ID:SGTC_1677|Compound:st019204|FD-Score:-3.26|P-value:6.35E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.22|P-value:6.65E-4 ID:SGTC_1891|Compound:st059073|FD-Score:3.14|P-value:8.88E-4 ID:SGTC_1896|Compound:st058441|FD-Score:-3.32|P-value:5.24E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.40|P-value:3.51E-4 ID:SGTC_2134|Compound:5324836|FD-Score:-4.64|P-value:2.17E-6 ID:SGTC_2300|Compound:7976697|FD-Score:-3.26|P-value:6.29E-4 ID:SGTC_2303|Compound:7774051|FD-Score:-3.72|P-value:1.16E-4 ID:SGTC_2305|Compound:7443522|FD-Score:-3.44|P-value:3.36E-4 ID:SGTC_2314|Compound:7727897|FD-Score:-3.42|P-value:3.67E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-6.46|P-value:7.77E-11 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:5.21|P-value:1.07E-7 ID:SGTC_2791|Compound:5663188|FD-Score:3.34|P-value:4.37E-4 ID:SGTC_2935|Compound:9038016|FD-Score:4.02|P-value:3.10E-5 ID:SGTC_3211|Compound:9128781|FD-Score:-3.94|P-value:4.83E-5 ID:SGTC_3325|Compound:9138760|FD-Score:-3.79|P-value:8.81E-5 ID:SGTC_3328|Compound:9140624|FD-Score:4.43|P-value:5.25E-6 ID:SGTC_3331|Compound:9141060|FD-Score:-4.25|P-value:1.31E-5 ID:SGTC_3349|Compound:9149703|FD-Score:3.16|P-value:8.15E-4 ID:SGTC_316|Compound:k060-0003|FD-Score:3.17|P-value:7.89E-4 ID:SGTC_340|Compound:k038-0059|FD-Score:3.26|P-value:5.80E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:-4.70|P-value:1.68E-6 ID:SGTC_817|Compound:1391-0715|FD-Score:3.21|P-value:6.88E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:3.48|P-value:2.62E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:3.79|P-value:8.15E-5 ID:SGTC_875|Compound:0709-0210|FD-Score:3.52|P-value:2.24E-4 ID:SGTC_945|Compound:3381-0768|FD-Score:4.44|P-value:4.96E-6 ID:SGTC_946|Compound:3434-1334|FD-Score:4.36|P-value:7.24E-6 ID:SGTC_958|Compound:1222-0007|FD-Score:3.40|P-value:3.51E-4 ID:SGTC_1014|Compound:4130-3005|FD-Score:3.21|P-value:6.89E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:8.19|P-value:1.91E-16 ID:SGTC_1385|Compound:3091-4834|FD-Score:-3.76|P-value:9.86E-5 ID:SGTC_1391|Compound:3442-0225|FD-Score:-3.36|P-value:4.42E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:3.51|P-value:2.34E-4 ID:SGTC_1675|Compound:st018485|FD-Score:-3.21|P-value:7.50E-4 ID:SGTC_1677|Compound:st019204|FD-Score:-3.26|P-value:6.35E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.22|P-value:6.65E-4 ID:SGTC_1891|Compound:st059073|FD-Score:3.14|P-value:8.88E-4 ID:SGTC_1896|Compound:st058441|FD-Score:-3.32|P-value:5.24E-4 ID:SGTC_2130|Compound:NSC 7|FD-Score:3.40|P-value:3.51E-4 ID:SGTC_2134|Compound:5324836|FD-Score:-4.64|P-value:2.17E-6 ID:SGTC_2300|Compound:7976697|FD-Score:-3.26|P-value:6.29E-4 ID:SGTC_2303|Compound:7774051|FD-Score:-3.72|P-value:1.16E-4 ID:SGTC_2305|Compound:7443522|FD-Score:-3.44|P-value:3.36E-4 ID:SGTC_2314|Compound:7727897|FD-Score:-3.42|P-value:3.67E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-6.46|P-value:7.77E-11 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:5.21|P-value:1.07E-7 ID:SGTC_2791|Compound:5663188|FD-Score:3.34|P-value:4.37E-4 ID:SGTC_2935|Compound:9038016|FD-Score:4.02|P-value:3.10E-5 ID:SGTC_3211|Compound:9128781|FD-Score:-3.94|P-value:4.83E-5 ID:SGTC_3325|Compound:9138760|FD-Score:-3.79|P-value:8.81E-5 ID:SGTC_3328|Compound:9140624|FD-Score:4.43|P-value:5.25E-6 ID:SGTC_3331|Compound:9141060|FD-Score:-4.25|P-value:1.31E-5 ID:SGTC_3349|Compound:9149703|FD-Score:3.16|P-value:8.15E-4

Top fitness defect scores for YLR082C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1248 0469-0687 8.19 58.6 µM 1.91E-16 1 1.25E-16
2 SGTC_2588 indole-3-carbinol 5.21 100.0 µM 1.07E-7 15 9.25E-8
3 SGTC_945 3381-0768 4.44 11.3 µM 4.96E-6 endomembrane recycling 25 4.49E-6
4 SGTC_3328 9140624 4.43 29.0 µM 5.25E-6 10 4.75E-6
5 SGTC_946 3434-1334 4.36 87.4 µM 7.24E-6 20 6.58E-6
6 SGTC_2935 9038016 4.02 30.0 µM 3.10E-5 20 2.87E-5
7 SGTC_871 0574-0004 3.79 149.0 µM 8.15E-5 54 7.62E-5
8 SGTC_875 0709-0210 3.52 25.7 µM 2.24E-4 fatty acid desaturase (OLE1) 48 2.12E-4
9 SGTC_1454 4239-0054 3.51 42.6 µM 2.34E-4 48 2.22E-4
10 SGTC_865 0488-0499 3.48 301.0 µM 2.62E-4 29 2.48E-4
11 SGTC_2130 NSC 7 3.40 2.5 µM 3.51E-4 copper-dependent oxidative stress 54 3.34E-4
12 SGTC_958 1222-0007 3.40 33.0 µM 3.51E-4 48 3.34E-4
13 SGTC_2791 5663188 3.34 71.4 µM 4.37E-4 RPP1 & pyrimidine depletion 45 4.16E-4
14 SGTC_340 k038-0059 3.26 454.0 µM 5.80E-4 ubiquinone biosynthesis & proteasome 67 5.55E-4
15 SGTC_1750 st081859 3.22 2.4 µM 6.65E-4 endomembrane recycling 231 6.37E-4
16 SGTC_817 1391-0715 3.21 46.0 µM 6.88E-4 40 6.59E-4
17 SGTC_1014 4130-3005 3.21 69.3 µM 6.89E-4 50 6.60E-4
18 SGTC_316 k060-0003 3.17 58.6 µM 7.89E-4 38 7.57E-4
19 SGTC_3349 9149703 3.16 23.0 µM 8.15E-4 37 7.82E-4
20 SGTC_1891 st059073 3.14 26.2 µM 8.88E-4 TSC3-RPN4 53 8.53E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.134 7.99E-15 YGR003W CUL3 hom Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3
0.127 1.36E-13 YDR210W_p YDR210W_p hom Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.126 2.27E-13 YDR031W MIC14 hom Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
0.114 3.61E-11 YLR060W FRS1 het Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
0.114 3.75E-11 YIL108W YIL108W hom Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress
0.113 5.01E-11 YML100W-A_p YML100W-A_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.111 1.29E-10 YMR095C SNO1 hom Protein of unconfirmed function, involved in pyridoxine metabolism; expression is induced during stationary phase; forms a putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase
0.109 2.78E-10 YOR202W HIS3 hom Imidazoleglycerol-phosphate dehydratase, catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p
0.108 4.23E-10 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.105 9.03E-10 YLR300W EXG1 hom Major exo-1,3-beta-glucanase of the cell wall, involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes
0.104 1.31E-9 YPR153W YPR153W hom Putative protein of unknown function
0.103 2.16E-9 YMR215W GAS3 hom Low abundance, possibly inactive member of the GAS family of GPI-containing proteins; putative 1,3-beta-glucanosyltransferase with similarity to other GAS family members; localizes to the cell wall; mRNA induced during sporulation
0.102 3.59E-9 YPR079W MRL1 hom Membrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress
0.101 4.04E-9 YMR008C PLB1 hom Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol
0.099 9.26E-9 YMR133W REC114 hom Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division