YLR100W / ERG27

3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR100W / ERG27

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:3.03|P-value:7.20E-4 ID:SGTC_74|Compound:000s-0487|FD-Score:3.28|P-value:2.85E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:3.36|P-value:2.05E-4 ID:SGTC_194|Compound:1486-1323|FD-Score:3.38|P-value:1.93E-4 ID:SGTC_216|Compound:k038-0035|FD-Score:3.25|P-value:3.13E-4 ID:SGTC_338|Compound:4055-0136|FD-Score:3.02|P-value:7.45E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.94|P-value:1.66E-5 ID:SGTC_664|Compound:1222-0054|FD-Score:11.00|P-value:1.58E-31 ID:SGTC_670|Compound:4204-0025|FD-Score:3.24|P-value:3.24E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:3.53|P-value:1.01E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:4.72|P-value:3.48E-7 ID:SGTC_714|Compound:4296-0578|FD-Score:-3.17|P-value:4.18E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:-3.30|P-value:2.46E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:2.97|P-value:9.09E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:4.05|P-value:1.04E-5 ID:SGTC_786|Compound:4544-0069|FD-Score:3.12|P-value:5.09E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:4.39|P-value:1.93E-6 ID:SGTC_1052|Compound:3253-3998|FD-Score:-3.93|P-value:1.67E-5 ID:SGTC_1054|Compound:3429-0080|FD-Score:3.50|P-value:1.17E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:-4.75|P-value:2.70E-7 ID:SGTC_1141|Compound:3253-0389|FD-Score:-3.29|P-value:2.64E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.19|P-value:4.05E-4 ID:SGTC_1364|Compound:1653-0028|FD-Score:3.28|P-value:2.81E-4 ID:SGTC_1383|Compound:3049-0032|FD-Score:3.56|P-value:8.89E-5 ID:SGTC_1653|Compound:st012112|FD-Score:-3.42|P-value:1.57E-4 ID:SGTC_1668|Compound:st014914|FD-Score:3.42|P-value:1.62E-4 ID:SGTC_1779|Compound:st039427|FD-Score:3.28|P-value:2.80E-4 ID:SGTC_1805|Compound:st050013|FD-Score:3.10|P-value:5.63E-4 ID:SGTC_1820|Compound:st053765|FD-Score:8.22|P-value:2.48E-18 ID:SGTC_1823|Compound:3-hydroxy-2-naphthoic acid|FD-Score:4.29|P-value:3.20E-6 ID:SGTC_1847|Compound:st056231|FD-Score:3.19|P-value:4.03E-4 ID:SGTC_1861|Compound:st057647|FD-Score:4.27|P-value:3.60E-6 ID:SGTC_1945|Compound:st075991|FD-Score:2.99|P-value:8.48E-4 ID:SGTC_1981|Compound:st071826|FD-Score:-3.23|P-value:3.29E-4 ID:SGTC_1993|Compound:st073995|FD-Score:3.50|P-value:1.16E-4 ID:SGTC_2132|Compound:5317594|FD-Score:-3.63|P-value:6.53E-5 ID:SGTC_2157|Compound:5649910|FD-Score:3.12|P-value:5.18E-4 ID:SGTC_2493|Compound:resveratrol|FD-Score:3.05|P-value:6.62E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:3.02|P-value:7.52E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-3.63|P-value:6.51E-5 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-3.28|P-value:2.73E-4 ID:SGTC_2790|Compound:5423970|FD-Score:3.16|P-value:4.38E-4 ID:SGTC_2928|Compound:9011484|FD-Score:3.62|P-value:7.01E-5 ID:SGTC_2986|Compound:9022445|FD-Score:3.72|P-value:4.54E-5 ID:SGTC_3049|Compound:9092223|FD-Score:-4.07|P-value:8.74E-6 ID:SGTC_3073|Compound:9114972|FD-Score:3.28|P-value:2.78E-4 ID:SGTC_3151|Compound:9099821|FD-Score:3.14|P-value:4.89E-4 ID:SGTC_3202|Compound:9113761|FD-Score:3.13|P-value:4.97E-4 ID:SGTC_3208|Compound:9114115|FD-Score:-4.11|P-value:7.17E-6 ID:SGTC_64|Compound:000l-0018|FD-Score:3.03|P-value:7.20E-4 ID:SGTC_74|Compound:000s-0487|FD-Score:3.28|P-value:2.85E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:3.36|P-value:2.05E-4 ID:SGTC_194|Compound:1486-1323|FD-Score:3.38|P-value:1.93E-4 ID:SGTC_216|Compound:k038-0035|FD-Score:3.25|P-value:3.13E-4 ID:SGTC_338|Compound:4055-0136|FD-Score:3.02|P-value:7.45E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:-3.94|P-value:1.66E-5 ID:SGTC_664|Compound:1222-0054|FD-Score:11.00|P-value:1.58E-31 ID:SGTC_670|Compound:4204-0025|FD-Score:3.24|P-value:3.24E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:3.53|P-value:1.01E-4 ID:SGTC_676|Compound:falcarinol|FD-Score:4.72|P-value:3.48E-7 ID:SGTC_714|Compound:4296-0578|FD-Score:-3.17|P-value:4.18E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:-3.30|P-value:2.46E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:2.97|P-value:9.09E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:4.05|P-value:1.04E-5 ID:SGTC_786|Compound:4544-0069|FD-Score:3.12|P-value:5.09E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:4.39|P-value:1.93E-6 ID:SGTC_1052|Compound:3253-3998|FD-Score:-3.93|P-value:1.67E-5 ID:SGTC_1054|Compound:3429-0080|FD-Score:3.50|P-value:1.17E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:-4.75|P-value:2.70E-7 ID:SGTC_1141|Compound:3253-0389|FD-Score:-3.29|P-value:2.64E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.19|P-value:4.05E-4 ID:SGTC_1364|Compound:1653-0028|FD-Score:3.28|P-value:2.81E-4 ID:SGTC_1383|Compound:3049-0032|FD-Score:3.56|P-value:8.89E-5 ID:SGTC_1653|Compound:st012112|FD-Score:-3.42|P-value:1.57E-4 ID:SGTC_1668|Compound:st014914|FD-Score:3.42|P-value:1.62E-4 ID:SGTC_1779|Compound:st039427|FD-Score:3.28|P-value:2.80E-4 ID:SGTC_1805|Compound:st050013|FD-Score:3.10|P-value:5.63E-4 ID:SGTC_1820|Compound:st053765|FD-Score:8.22|P-value:2.48E-18 ID:SGTC_1823|Compound:3-hydroxy-2-naphthoic acid|FD-Score:4.29|P-value:3.20E-6 ID:SGTC_1847|Compound:st056231|FD-Score:3.19|P-value:4.03E-4 ID:SGTC_1861|Compound:st057647|FD-Score:4.27|P-value:3.60E-6 ID:SGTC_1945|Compound:st075991|FD-Score:2.99|P-value:8.48E-4 ID:SGTC_1981|Compound:st071826|FD-Score:-3.23|P-value:3.29E-4 ID:SGTC_1993|Compound:st073995|FD-Score:3.50|P-value:1.16E-4 ID:SGTC_2132|Compound:5317594|FD-Score:-3.63|P-value:6.53E-5 ID:SGTC_2157|Compound:5649910|FD-Score:3.12|P-value:5.18E-4 ID:SGTC_2493|Compound:resveratrol|FD-Score:3.05|P-value:6.62E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:3.02|P-value:7.52E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-3.63|P-value:6.51E-5 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-3.28|P-value:2.73E-4 ID:SGTC_2790|Compound:5423970|FD-Score:3.16|P-value:4.38E-4 ID:SGTC_2928|Compound:9011484|FD-Score:3.62|P-value:7.01E-5 ID:SGTC_2986|Compound:9022445|FD-Score:3.72|P-value:4.54E-5 ID:SGTC_3049|Compound:9092223|FD-Score:-4.07|P-value:8.74E-6 ID:SGTC_3073|Compound:9114972|FD-Score:3.28|P-value:2.78E-4 ID:SGTC_3151|Compound:9099821|FD-Score:3.14|P-value:4.89E-4 ID:SGTC_3202|Compound:9113761|FD-Score:3.13|P-value:4.97E-4 ID:SGTC_3208|Compound:9114115|FD-Score:-4.11|P-value:7.17E-6

Top fitness defect scores for YLR100W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_664 1222-0054 11.00 14.9 µM 1.58E-31 sphingolipid biosynthesis & PDR1 1 1.22E-28 4.66
2 SGTC_1820 st053765 8.22 18.6 µM 2.48E-18 RPP1 & pyrimidine depletion 1 1.00E-16 1.41
3 SGTC_676 falcarinol 4.72 140.0 µM 3.48E-7 5 1.20E-6 0.07
4 SGTC_841 0251-0215 4.39 91.2 µM 1.93E-6 2 5.66E-6 0.79
5 SGTC_1823 3-hydroxy-2-naphthoic acid 4.29 106.0 µM 3.20E-6 3 8.94E-6 0.51
6 SGTC_1861 st057647 4.27 17.4 µM 3.60E-6 5 9.96E-6 0.51
7 SGTC_778 3456-2434 4.05 196.0 µM 1.04E-5 mitochondrial response to ROS 6 2.61E-5 0.05
8 SGTC_2986 9022445 3.72 71.4 µM 4.54E-5 7 9.92E-5 0.26
9 SGTC_2928 9011484 3.62 9.8 µM 7.01E-5 heme biosynthesis & mitochondrial translocase 35 1.47E-4 0.06
10 SGTC_1383 3049-0032 3.56 20.7 µM 8.89E-5 60S ribosome export 14 1.83E-4 0.08
11 SGTC_674 0498-0004 3.53 451.0 µM 1.01E-4 heme requiring 29 2.05E-4 0.03
12 SGTC_1993 st073995 3.50 70.8 µM 1.16E-4 8 2.32E-4 0.03
13 SGTC_1054 3429-0080 3.50 167.0 µM 1.17E-4 7 2.34E-4 0.11
14 SGTC_1668 st014914 3.42 51.6 µM 1.62E-4 RPP1 & pyrimidine depletion 10 3.15E-4 0.08
15 SGTC_194 1486-1323 3.38 56.9 µM 1.93E-4 13 3.68E-4 0.13
16 SGTC_152 4469-0003 3.36 551.2 µM 2.05E-4 24 3.90E-4 0.00
17 SGTC_3073 9114972 3.28 49.5 µM 2.78E-4 60S ribosome export 11 5.14E-4 0.18
18 SGTC_1779 st039427 3.28 55.0 µM 2.80E-4 7 5.18E-4 0.16
19 SGTC_1364 1653-0028 3.28 37.6 µM 2.81E-4 7 5.19E-4 0.15
20 SGTC_74 000s-0487 3.28 160.3 µM 2.85E-4 7 5.25E-4 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.223 3.24E-39 YLR101C_d YLR101C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W
0.152 8.51E-19 YOL050C_d YOL050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.119 4.68E-12 YJR005W APL1 hom Beta-adaptin, large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex
0.118 6.22E-12 YHR021W-A_p ECM12_p hom Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
0.108 3.78E-10 YGR245C SDA1 het Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis
0.100 6.82E-9 YGL013C PDR1 hom Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
0.086 7.01E-7 YDR468C TLG1 het Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p
0.084 9.53E-7 YNR054C ESF2 het Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome
0.084 1.09E-6 YOR291W YPK9 hom Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
0.083 1.67E-6 YJL061W NUP82 het Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p)
0.082 2.12E-6 YGR264C MES1 het Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs
0.080 3.03E-6 YOR021C SFM1 hom SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism
0.079 4.14E-6 YMR034C_p YMR034C_p hom Putative transporter, member of the SLC10 carrier family; identified in a transposon mutagenesis screen as a gene involved in azole resistance; YMR034C is not an essential gene
0.079 5.20E-6 YJL194W CDC6 het Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.077 7.47E-6 YJL032W_d YJL032W_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase