YLR104W_p / LCL2

Putative protein of unknown function; mutant is deficient in amounts of cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD)

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR104W / LCL2

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:-3.90|P-value:2.86E-5 ID:SGTC_112|Compound:phenothiazine|FD-Score:-3.04|P-value:8.23E-4 ID:SGTC_286|Compound:k060-0067|FD-Score:4.33|P-value:4.64E-6 ID:SGTC_287|Compound:k060-0039|FD-Score:4.30|P-value:5.44E-6 ID:SGTC_313|Compound:3474-0040|FD-Score:3.64|P-value:9.94E-5 ID:SGTC_381|Compound:3461-2219|FD-Score:4.43|P-value:2.93E-6 ID:SGTC_408|Compound:cantharidin|FD-Score:-3.24|P-value:4.05E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:5.37|P-value:1.92E-8 ID:SGTC_744|Compound:1594-0068|FD-Score:3.58|P-value:1.27E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:-3.24|P-value:4.04E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:-3.53|P-value:1.34E-4 ID:SGTC_1401|Compound:3807-4679|FD-Score:-3.43|P-value:1.97E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.20|P-value:5.34E-4 ID:SGTC_1870|Compound:st057514|FD-Score:-4.48|P-value:1.89E-6 ID:SGTC_1876|Compound:myriocin|FD-Score:3.72|P-value:7.23E-5 ID:SGTC_1886|Compound:st057356|FD-Score:3.61|P-value:1.11E-4 ID:SGTC_1942|Compound:st076330|FD-Score:-5.10|P-value:7.32E-8 ID:SGTC_2114|Compound:5491910|FD-Score:3.07|P-value:8.50E-4 ID:SGTC_2282|Compound:7944268|FD-Score:3.53|P-value:1.55E-4 ID:SGTC_2340|Compound:7987427|FD-Score:3.04|P-value:9.69E-4 ID:SGTC_2385|Compound:9074692|FD-Score:3.17|P-value:6.06E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:-3.52|P-value:1.38E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:-4.02|P-value:1.64E-5 ID:SGTC_2719|Compound:novobiocin|FD-Score:3.99|P-value:2.27E-5 ID:SGTC_2744|Compound:captopril|FD-Score:3.65|P-value:9.51E-5 ID:SGTC_2754|Compound:granisetron|FD-Score:3.82|P-value:4.66E-5 ID:SGTC_2972|Compound:9093619|FD-Score:4.19|P-value:9.09E-6 ID:SGTC_2973|Compound:9094665|FD-Score:3.90|P-value:3.26E-5 ID:SGTC_3059|Compound:1327-0034|FD-Score:4.60|P-value:1.28E-6 ID:SGTC_3085|Compound:9117376|FD-Score:3.78|P-value:5.62E-5 ID:SGTC_3126|Compound:9127922|FD-Score:-3.95|P-value:2.31E-5 ID:SGTC_3145|Compound:9085516|FD-Score:-3.27|P-value:3.58E-4 ID:SGTC_3160|Compound:9100216|FD-Score:-3.15|P-value:5.70E-4 ID:SGTC_3169|Compound:9102970|FD-Score:3.63|P-value:1.04E-4 ID:SGTC_3224|Compound:9130643|FD-Score:-5.61|P-value:3.72E-9 ID:SGTC_37|Compound:0566-0070|FD-Score:-3.90|P-value:2.86E-5 ID:SGTC_112|Compound:phenothiazine|FD-Score:-3.04|P-value:8.23E-4 ID:SGTC_286|Compound:k060-0067|FD-Score:4.33|P-value:4.64E-6 ID:SGTC_287|Compound:k060-0039|FD-Score:4.30|P-value:5.44E-6 ID:SGTC_313|Compound:3474-0040|FD-Score:3.64|P-value:9.94E-5 ID:SGTC_381|Compound:3461-2219|FD-Score:4.43|P-value:2.93E-6 ID:SGTC_408|Compound:cantharidin|FD-Score:-3.24|P-value:4.05E-4 ID:SGTC_588|Compound:3970-1144|FD-Score:5.37|P-value:1.92E-8 ID:SGTC_744|Compound:1594-0068|FD-Score:3.58|P-value:1.27E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:-3.24|P-value:4.04E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:-3.53|P-value:1.34E-4 ID:SGTC_1401|Compound:3807-4679|FD-Score:-3.43|P-value:1.97E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.20|P-value:5.34E-4 ID:SGTC_1870|Compound:st057514|FD-Score:-4.48|P-value:1.89E-6 ID:SGTC_1876|Compound:myriocin|FD-Score:3.72|P-value:7.23E-5 ID:SGTC_1886|Compound:st057356|FD-Score:3.61|P-value:1.11E-4 ID:SGTC_1942|Compound:st076330|FD-Score:-5.10|P-value:7.32E-8 ID:SGTC_2114|Compound:5491910|FD-Score:3.07|P-value:8.50E-4 ID:SGTC_2282|Compound:7944268|FD-Score:3.53|P-value:1.55E-4 ID:SGTC_2340|Compound:7987427|FD-Score:3.04|P-value:9.69E-4 ID:SGTC_2385|Compound:9074692|FD-Score:3.17|P-value:6.06E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:-3.52|P-value:1.38E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:-4.02|P-value:1.64E-5 ID:SGTC_2719|Compound:novobiocin|FD-Score:3.99|P-value:2.27E-5 ID:SGTC_2744|Compound:captopril|FD-Score:3.65|P-value:9.51E-5 ID:SGTC_2754|Compound:granisetron|FD-Score:3.82|P-value:4.66E-5 ID:SGTC_2972|Compound:9093619|FD-Score:4.19|P-value:9.09E-6 ID:SGTC_2973|Compound:9094665|FD-Score:3.90|P-value:3.26E-5 ID:SGTC_3059|Compound:1327-0034|FD-Score:4.60|P-value:1.28E-6 ID:SGTC_3085|Compound:9117376|FD-Score:3.78|P-value:5.62E-5 ID:SGTC_3126|Compound:9127922|FD-Score:-3.95|P-value:2.31E-5 ID:SGTC_3145|Compound:9085516|FD-Score:-3.27|P-value:3.58E-4 ID:SGTC_3160|Compound:9100216|FD-Score:-3.15|P-value:5.70E-4 ID:SGTC_3169|Compound:9102970|FD-Score:3.63|P-value:1.04E-4 ID:SGTC_3224|Compound:9130643|FD-Score:-5.61|P-value:3.72E-9

Top fitness defect scores for YLR104W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_588 3970-1144 5.37 52.9 µM 1.92E-8 7 3.95E-8
2 SGTC_3059 1327-0034 4.60 3.0 µM 1.28E-6 40 2.16E-6
3 SGTC_381 3461-2219 4.43 42.7 µM 2.93E-6 copper-dependent oxidative stress 14 4.74E-6
4 SGTC_286 k060-0067 4.33 22.9 µM 4.64E-6 tubulin folding & SWR complex 20 7.35E-6
5 SGTC_287 k060-0039 4.30 29.6 µM 5.44E-6 6 8.54E-6
6 SGTC_2972 9093619 4.19 59.9 µM 9.09E-6 18 1.39E-5
7 SGTC_2719 novobiocin 3.99 32.6 µM 2.27E-5 3 3.33E-5
8 SGTC_2973 9094665 3.90 18.0 µM 3.26E-5 NEO1 31 4.72E-5
9 SGTC_2754 granisetron 3.82 64.0 µM 4.66E-5 35 6.63E-5
10 SGTC_3085 9117376 3.78 49.5 µM 5.62E-5 14 7.92E-5
11 SGTC_1876 myriocin 3.72 605.0 nM 7.23E-5 42 1.01E-4
12 SGTC_2744 captopril 3.65 92.0 µM 9.51E-5 60S ribosome export 16 1.31E-4
13 SGTC_313 3474-0040 3.64 5.0 µM 9.94E-5 NEO1-PIK1 86 1.36E-4
14 SGTC_3169 9102970 3.63 49.5 µM 1.04E-4 RPP1 & pyrimidine depletion 7 1.42E-4
15 SGTC_1886 st057356 3.61 49.9 µM 1.11E-4 60S ribosome export 28 1.52E-4
16 SGTC_744 1594-0068 3.58 283.0 µM 1.27E-4 amide catabolism 40 1.72E-4
17 SGTC_2282 7944268 3.53 200.0 µM 1.55E-4 RPP1 & pyrimidine depletion 52 2.09E-4
18 SGTC_1562 morin hydrate 3.20 59.1 µM 5.34E-4 44 6.80E-4
19 SGTC_2385 9074692 3.17 200.0 µM 6.06E-4 73 7.67E-4
20 SGTC_2114 5491910 3.07 81.2 µM 8.50E-4 45 0.00106

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.150 2.29E-18 YGR212W SLI1 hom N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin
0.129 5.52E-14 YFL011W HXT10 hom Putative hexose transporter, expressed at low levels and expression is repressed by glucose
0.110 1.99E-10 YGR213C RTA1 hom Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein
0.102 3.43E-9 YOR057W SGT1 het Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress
0.087 4.68E-7 YDR130C FIN1 hom Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress
0.086 5.26E-7 YBR125C PTC4 hom Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity
0.085 7.71E-7 YOR343C_d YOR343C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.085 8.00E-7 YKR091W SRL3 hom Cytoplasmic protein that, when overexpressed, suppresses the lethality of a rad53 null mutation; potential Cdc28p substrate
0.084 9.95E-7 YOL036W_p YOL036W_p hom Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication
0.083 1.55E-6 YDR536W STL1 hom Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock
0.082 2.26E-6 YDR071C PAA1 hom Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication
0.081 2.57E-6 YMR296C LCB1 het Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
0.079 5.02E-6 YOR379C_d YOR379C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W
0.076 1.16E-5 YMR025W CSI1 hom Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome
0.073 2.19E-5 YML023C NSE5 het Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair