YLR106C / MDN1

Huge dynein-related AAA-type ATPase (midasin), forms extended pre-60S particle with the Rix1 complex (Rix1p-Ipi1p-Ipi3p); acts in removal of ribosomal biogenesis factors at successive steps of pre-60S assembly and export from nucleus

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR106C / MDN1

Click on Significant Values for Screen Details ID:SGTC_88|Compound:0180-0332|FD-Score:-2.80|P-value:8.92E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:2.81|P-value:8.04E-4 ID:SGTC_251|Compound:melphalan|FD-Score:3.32|P-value:9.75E-5 ID:SGTC_270|Compound:tamoxifen|FD-Score:3.03|P-value:3.46E-4 ID:SGTC_276|Compound:colchicine|FD-Score:2.98|P-value:4.12E-4 ID:SGTC_348|Compound:k064-0012|FD-Score:3.09|P-value:2.68E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:-3.97|P-value:4.68E-6 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:6.09|P-value:4.46E-12 ID:SGTC_425|Compound:3825-7017|FD-Score:3.62|P-value:2.48E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-7.70|P-value:3.65E-18 ID:SGTC_462|Compound:ag-879|FD-Score:3.57|P-value:3.10E-5 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:4.57|P-value:1.54E-7 ID:SGTC_495|Compound:decylubiquinone|FD-Score:2.97|P-value:4.33E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:4.54|P-value:1.82E-7 ID:SGTC_519|Compound:k048-0011|FD-Score:3.50|P-value:4.34E-5 ID:SGTC_749|Compound:1000-0129|FD-Score:3.08|P-value:2.80E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:2.87|P-value:6.42E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:-4.73|P-value:6.49E-8 ID:SGTC_1075|Compound:aripiprazole|FD-Score:2.91|P-value:5.41E-4 ID:SGTC_1085|Compound:doxorubicin|FD-Score:3.68|P-value:1.83E-5 ID:SGTC_1384|Compound:3089-0840|FD-Score:-4.22|P-value:1.18E-6 ID:SGTC_1452|Compound:4194-0072|FD-Score:-3.26|P-value:1.34E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:2.97|P-value:4.29E-4 ID:SGTC_1621|Compound:st003705|FD-Score:2.90|P-value:5.73E-4 ID:SGTC_1913|Compound:st060213|FD-Score:-4.08|P-value:2.62E-6 ID:SGTC_1999|Compound:st074831|FD-Score:4.69|P-value:7.48E-8 ID:SGTC_2025|Compound:5147754|FD-Score:-2.79|P-value:9.17E-4 ID:SGTC_2037|Compound:5169046|FD-Score:3.12|P-value:2.30E-4 ID:SGTC_2047|Compound:4141938|FD-Score:-3.05|P-value:3.36E-4 ID:SGTC_2056|Compound:5235370|FD-Score:3.19|P-value:1.71E-4 ID:SGTC_2102|Compound:5377816|FD-Score:4.15|P-value:1.68E-6 ID:SGTC_2106|Compound:5357045|FD-Score:2.94|P-value:4.85E-4 ID:SGTC_2132|Compound:5317594|FD-Score:-2.94|P-value:5.23E-4 ID:SGTC_2134|Compound:5324836|FD-Score:-2.79|P-value:9.24E-4 ID:SGTC_2284|Compound:7945075|FD-Score:-2.83|P-value:8.04E-4 ID:SGTC_2287|Compound:7945887|FD-Score:-3.39|P-value:7.74E-5 ID:SGTC_2296|Compound:7971656|FD-Score:2.99|P-value:4.01E-4 ID:SGTC_2323|Compound:6148992|FD-Score:-2.88|P-value:6.46E-4 ID:SGTC_2439|Compound:5492030|FD-Score:-3.32|P-value:1.05E-4 ID:SGTC_2441|Compound:5730946|FD-Score:-6.02|P-value:8.47E-12 ID:SGTC_2478|Compound:5763493|FD-Score:-4.02|P-value:3.63E-6 ID:SGTC_2480|Compound:5767542|FD-Score:-3.48|P-value:4.98E-5 ID:SGTC_2606|Compound:farnesol|FD-Score:-3.00|P-value:4.05E-4 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:-3.28|P-value:1.25E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-3.42|P-value:6.65E-5 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-2.88|P-value:6.58E-4 ID:SGTC_2842|Compound:9000675|FD-Score:-4.78|P-value:4.70E-8 ID:SGTC_2859|Compound:9028643|FD-Score:2.92|P-value:5.29E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-4.22|P-value:1.21E-6 ID:SGTC_2972|Compound:9093619|FD-Score:-2.82|P-value:8.27E-4 ID:SGTC_3074|Compound:9116018|FD-Score:-3.31|P-value:1.10E-4 ID:SGTC_3085|Compound:9117376|FD-Score:3.28|P-value:1.18E-4 ID:SGTC_3090|Compound:9117696|FD-Score:2.85|P-value:7.00E-4 ID:SGTC_3137|Compound:9097352|FD-Score:3.12|P-value:2.31E-4 ID:SGTC_3178|Compound:9107506|FD-Score:-3.20|P-value:1.77E-4 ID:SGTC_3233|Compound:9132340|FD-Score:3.02|P-value:3.61E-4 ID:SGTC_3286|Compound:9117135|FD-Score:-2.86|P-value:7.12E-4 ID:SGTC_3320|Compound:9137005|FD-Score:3.15|P-value:2.04E-4 ID:SGTC_3327|Compound:9140648|FD-Score:3.07|P-value:2.91E-4 ID:SGTC_88|Compound:0180-0332|FD-Score:-2.80|P-value:8.92E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:2.81|P-value:8.04E-4 ID:SGTC_251|Compound:melphalan|FD-Score:3.32|P-value:9.75E-5 ID:SGTC_270|Compound:tamoxifen|FD-Score:3.03|P-value:3.46E-4 ID:SGTC_276|Compound:colchicine|FD-Score:2.98|P-value:4.12E-4 ID:SGTC_348|Compound:k064-0012|FD-Score:3.09|P-value:2.68E-4 ID:SGTC_421|Compound:streptovitacin|FD-Score:-3.97|P-value:4.68E-6 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:6.09|P-value:4.46E-12 ID:SGTC_425|Compound:3825-7017|FD-Score:3.62|P-value:2.48E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-7.70|P-value:3.65E-18 ID:SGTC_462|Compound:ag-879|FD-Score:3.57|P-value:3.10E-5 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:4.57|P-value:1.54E-7 ID:SGTC_495|Compound:decylubiquinone|FD-Score:2.97|P-value:4.33E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:4.54|P-value:1.82E-7 ID:SGTC_519|Compound:k048-0011|FD-Score:3.50|P-value:4.34E-5 ID:SGTC_749|Compound:1000-0129|FD-Score:3.08|P-value:2.80E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:2.87|P-value:6.42E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:-4.73|P-value:6.49E-8 ID:SGTC_1075|Compound:aripiprazole|FD-Score:2.91|P-value:5.41E-4 ID:SGTC_1085|Compound:doxorubicin|FD-Score:3.68|P-value:1.83E-5 ID:SGTC_1384|Compound:3089-0840|FD-Score:-4.22|P-value:1.18E-6 ID:SGTC_1452|Compound:4194-0072|FD-Score:-3.26|P-value:1.34E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:2.97|P-value:4.29E-4 ID:SGTC_1621|Compound:st003705|FD-Score:2.90|P-value:5.73E-4 ID:SGTC_1913|Compound:st060213|FD-Score:-4.08|P-value:2.62E-6 ID:SGTC_1999|Compound:st074831|FD-Score:4.69|P-value:7.48E-8 ID:SGTC_2025|Compound:5147754|FD-Score:-2.79|P-value:9.17E-4 ID:SGTC_2037|Compound:5169046|FD-Score:3.12|P-value:2.30E-4 ID:SGTC_2047|Compound:4141938|FD-Score:-3.05|P-value:3.36E-4 ID:SGTC_2056|Compound:5235370|FD-Score:3.19|P-value:1.71E-4 ID:SGTC_2102|Compound:5377816|FD-Score:4.15|P-value:1.68E-6 ID:SGTC_2106|Compound:5357045|FD-Score:2.94|P-value:4.85E-4 ID:SGTC_2132|Compound:5317594|FD-Score:-2.94|P-value:5.23E-4 ID:SGTC_2134|Compound:5324836|FD-Score:-2.79|P-value:9.24E-4 ID:SGTC_2284|Compound:7945075|FD-Score:-2.83|P-value:8.04E-4 ID:SGTC_2287|Compound:7945887|FD-Score:-3.39|P-value:7.74E-5 ID:SGTC_2296|Compound:7971656|FD-Score:2.99|P-value:4.01E-4 ID:SGTC_2323|Compound:6148992|FD-Score:-2.88|P-value:6.46E-4 ID:SGTC_2439|Compound:5492030|FD-Score:-3.32|P-value:1.05E-4 ID:SGTC_2441|Compound:5730946|FD-Score:-6.02|P-value:8.47E-12 ID:SGTC_2478|Compound:5763493|FD-Score:-4.02|P-value:3.63E-6 ID:SGTC_2480|Compound:5767542|FD-Score:-3.48|P-value:4.98E-5 ID:SGTC_2606|Compound:farnesol|FD-Score:-3.00|P-value:4.05E-4 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:-3.28|P-value:1.25E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-3.42|P-value:6.65E-5 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-2.88|P-value:6.58E-4 ID:SGTC_2842|Compound:9000675|FD-Score:-4.78|P-value:4.70E-8 ID:SGTC_2859|Compound:9028643|FD-Score:2.92|P-value:5.29E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-4.22|P-value:1.21E-6 ID:SGTC_2972|Compound:9093619|FD-Score:-2.82|P-value:8.27E-4 ID:SGTC_3074|Compound:9116018|FD-Score:-3.31|P-value:1.10E-4 ID:SGTC_3085|Compound:9117376|FD-Score:3.28|P-value:1.18E-4 ID:SGTC_3090|Compound:9117696|FD-Score:2.85|P-value:7.00E-4 ID:SGTC_3137|Compound:9097352|FD-Score:3.12|P-value:2.31E-4 ID:SGTC_3178|Compound:9107506|FD-Score:-3.20|P-value:1.77E-4 ID:SGTC_3233|Compound:9132340|FD-Score:3.02|P-value:3.61E-4 ID:SGTC_3286|Compound:9117135|FD-Score:-2.86|P-value:7.12E-4 ID:SGTC_3320|Compound:9137005|FD-Score:3.15|P-value:2.04E-4 ID:SGTC_3327|Compound:9140648|FD-Score:3.07|P-value:2.91E-4

Top fitness defect scores for YLR106C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_423 5-fluorouracil 6.09 457.0 µM 4.46E-12 exosome 12 5.56E-10 0.02
2 SGTC_1999 st074831 4.69 12.5 µM 7.48E-8 plasma membrane duress 7 1.37E-6 0.05
3 SGTC_475 α-linolenic acid 4.57 10.0 µM 1.54E-7 excess fatty acid 5 2.45E-6 0.63
4 SGTC_515 4092-1064 4.54 102.0 µM 1.82E-7 plasma membrane duress 13 2.80E-6 0.17
5 SGTC_2102 5377816 4.15 72.8 µM 1.68E-6 4 1.68E-5 0.34
6 SGTC_1085 doxorubicin 3.68 7.7 µM 1.83E-5 anthracycline transcription coupled DNA repair 9 1.15E-4 0.03
7 SGTC_425 3825-7017 3.62 13.1 µM 2.48E-5 12 1.47E-4 0.02
8 SGTC_462 ag-879 3.57 158.0 µM 3.10E-5 18 1.76E-4 0.05
9 SGTC_519 k048-0011 3.50 62.8 µM 4.34E-5 11 2.31E-4 0.07
10 SGTC_251 melphalan 3.32 456.5 µM 9.75E-5 DNA damage response 5 4.44E-4 0.12
11 SGTC_3085 9117376 3.28 49.5 µM 1.18E-4 13 5.20E-4 0.05
12 SGTC_2056 5235370 3.19 200.0 µM 1.71E-4 14 7.00E-4 0.15
13 SGTC_3320 9137005 3.15 35.1 µM 2.04E-4 endomembrane recycling 13 8.08E-4 0.16
14 SGTC_2037 5169046 3.12 200.0 µM 2.30E-4 60S ribosome export 11 8.89E-4 0.16
15 SGTC_3137 9097352 3.12 49.5 µM 2.31E-4 21 8.91E-4 0.14
16 SGTC_348 k064-0012 3.09 10.7 µM 2.68E-4 60S ribosome export 16 0.00101 0.03
17 SGTC_749 1000-0129 3.08 128.0 µM 2.80E-4 amide catabolism 9 0.00104 0.11
18 SGTC_3327 9140648 3.07 26.9 µM 2.91E-4 34 0.00108 0.00
19 SGTC_270 tamoxifen 3.03 5.5 µM 3.46E-4 NEO1 16 0.00124 0.01
20 SGTC_3233 9132340 3.02 49.5 µM 3.61E-4 20 0.00128 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.169 5.79E-23 YHL026C_p YHL026C_p hom Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History)
0.125 4.17E-13 YKL172W EBP2 het Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.119 5.45E-12 YLR397C AFG2 het ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; may be involved in degradation of aberrant mRNAs
0.117 1.17E-11 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.109 2.13E-10 YNL245C CWC25 het Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p
0.108 4.20E-10 YLR124W_d YLR124W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.101 4.02E-9 YLR193C UPS1 hom Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI
0.098 1.44E-8 YDR405W MRP20 hom Mitochondrial ribosomal protein of the large subunit
0.097 1.67E-8 YGL057C GEP7 hom Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.096 2.94E-8 YOR019W YOR019W hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments
0.093 7.03E-8 YML090W_d YML090W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source
0.088 3.65E-7 YJR017C ESS1 het Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated serine and threonine residues N-terminal to proline; regulates phosphorylation of the RNAP II large subunit (Rpo21p) C-terminal domain (CTD); associates with phospho-Ser5 form of RNAP II in vivo; regulates phosphorylation of Ser7 within CTD; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription and trimethylation of histone H3
0.084 1.26E-6 YDR147W EKI1 hom Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication
0.081 2.93E-6 YLR418C CDC73 hom Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress
0.080 3.56E-6 YMR072W ABF2 hom Mitochondrial DNA-binding protein involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation