YLR115W / CFT2

Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein.

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR115W / CFT2

Click on Significant Values for Screen Details ID:SGTC_90|Compound:0870-0863|FD-Score:-2.96|P-value:9.03E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:3.12|P-value:6.18E-4 ID:SGTC_364|Compound:0083-0097|FD-Score:3.37|P-value:2.41E-4 ID:SGTC_1200|Compound:1643-0193|FD-Score:-3.18|P-value:4.04E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:3.15|P-value:5.68E-4 ID:SGTC_1316|Compound:1272-0050|FD-Score:3.73|P-value:5.65E-5 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:3.39|P-value:2.21E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.03|P-value:7.17E-4 ID:SGTC_1358|Compound:1509-0022|FD-Score:2.99|P-value:9.80E-4 ID:SGTC_1410|Compound:3909-8106|FD-Score:3.51|P-value:1.41E-4 ID:SGTC_1424|Compound:4048-1941|FD-Score:-3.64|P-value:6.33E-5 ID:SGTC_1433|Compound:4060-0023|FD-Score:3.76|P-value:4.82E-5 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:3.71|P-value:5.99E-5 ID:SGTC_1604|Compound:st002052|FD-Score:3.16|P-value:5.39E-4 ID:SGTC_1674|Compound:st016616|FD-Score:4.27|P-value:4.66E-6 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.97|P-value:1.98E-5 ID:SGTC_1683|Compound:st010134|FD-Score:-4.37|P-value:2.16E-6 ID:SGTC_1737|Compound:st035557|FD-Score:-3.10|P-value:5.42E-4 ID:SGTC_1957|Compound:st077035|FD-Score:3.78|P-value:4.52E-5 ID:SGTC_1975|Compound:st070967|FD-Score:3.37|P-value:2.43E-4 ID:SGTC_2313|Compound:7726885|FD-Score:3.62|P-value:8.91E-5 ID:SGTC_2342|Compound:7990637|FD-Score:3.06|P-value:7.70E-4 ID:SGTC_2390|Compound:9097792|FD-Score:4.10|P-value:1.08E-5 ID:SGTC_2492|Compound:derrusnin|FD-Score:3.05|P-value:8.03E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.72|P-value:5.80E-5 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:3.44|P-value:1.83E-4 ID:SGTC_2704|Compound:st078022|FD-Score:3.25|P-value:3.81E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-3.54|P-value:9.85E-5 ID:SGTC_3208|Compound:9114115|FD-Score:-3.85|P-value:2.62E-5 ID:SGTC_90|Compound:0870-0863|FD-Score:-2.96|P-value:9.03E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:3.12|P-value:6.18E-4 ID:SGTC_364|Compound:0083-0097|FD-Score:3.37|P-value:2.41E-4 ID:SGTC_1200|Compound:1643-0193|FD-Score:-3.18|P-value:4.04E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:3.15|P-value:5.68E-4 ID:SGTC_1316|Compound:1272-0050|FD-Score:3.73|P-value:5.65E-5 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:3.39|P-value:2.21E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-3.03|P-value:7.17E-4 ID:SGTC_1358|Compound:1509-0022|FD-Score:2.99|P-value:9.80E-4 ID:SGTC_1410|Compound:3909-8106|FD-Score:3.51|P-value:1.41E-4 ID:SGTC_1424|Compound:4048-1941|FD-Score:-3.64|P-value:6.33E-5 ID:SGTC_1433|Compound:4060-0023|FD-Score:3.76|P-value:4.82E-5 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:3.71|P-value:5.99E-5 ID:SGTC_1604|Compound:st002052|FD-Score:3.16|P-value:5.39E-4 ID:SGTC_1674|Compound:st016616|FD-Score:4.27|P-value:4.66E-6 ID:SGTC_1679|Compound:4130-1276|FD-Score:3.97|P-value:1.98E-5 ID:SGTC_1683|Compound:st010134|FD-Score:-4.37|P-value:2.16E-6 ID:SGTC_1737|Compound:st035557|FD-Score:-3.10|P-value:5.42E-4 ID:SGTC_1957|Compound:st077035|FD-Score:3.78|P-value:4.52E-5 ID:SGTC_1975|Compound:st070967|FD-Score:3.37|P-value:2.43E-4 ID:SGTC_2313|Compound:7726885|FD-Score:3.62|P-value:8.91E-5 ID:SGTC_2342|Compound:7990637|FD-Score:3.06|P-value:7.70E-4 ID:SGTC_2390|Compound:9097792|FD-Score:4.10|P-value:1.08E-5 ID:SGTC_2492|Compound:derrusnin|FD-Score:3.05|P-value:8.03E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.72|P-value:5.80E-5 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:3.44|P-value:1.83E-4 ID:SGTC_2704|Compound:st078022|FD-Score:3.25|P-value:3.81E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-3.54|P-value:9.85E-5 ID:SGTC_3208|Compound:9114115|FD-Score:-3.85|P-value:2.62E-5

Top fitness defect scores for YLR115W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1674 st016616 4.27 23.6 µM 4.66E-6 6 9.59E-6 0.18
2 SGTC_2390 9097792 4.10 176.6 µM 1.08E-5 1 2.09E-5 0.28
3 SGTC_1679 4130-1276 3.97 6.7 µM 1.98E-5 Golgi 24 3.66E-5 0.06
4 SGTC_1957 st077035 3.78 62.4 µM 4.52E-5 1 7.88E-5 0.27
5 SGTC_1433 4060-0023 3.76 7.6 µM 4.82E-5 tubulin folding & SWR complex 3 8.38E-5 0.19
6 SGTC_1316 1272-0050 3.73 65.9 µM 5.65E-5 1 9.70E-5 0.33
7 SGTC_2568 diosmetin 3.72 100.0 µM 5.80E-5 14 9.94E-5 0.15
8 SGTC_1575 cyproterone acetate 3.71 48.0 µM 5.99E-5 8 1.02E-4 0.15
9 SGTC_2313 7726885 3.62 200.0 µM 8.91E-5 RPP1 & pyrimidine depletion 8 1.48E-4 0.04
10 SGTC_1410 3909-8106 3.51 77.9 µM 1.41E-4 2 2.27E-4 0.09
11 SGTC_2632 3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone 3.44 100.0 µM 1.83E-4 6 2.89E-4 0.33
12 SGTC_1349 flufenamic acid 3.39 69.0 µM 2.21E-4 5 3.45E-4 0.10
13 SGTC_364 0083-0097 3.37 67.7 µM 2.41E-4 13 3.73E-4 0.05
14 SGTC_1975 st070967 3.37 30.8 µM 2.43E-4 18 3.77E-4 0.00
15 SGTC_2704 st078022 3.25 71.1 µM 3.81E-4 8 5.71E-4 0.14
16 SGTC_1604 st002052 3.16 72.4 µM 5.39E-4 TSC3-RPN4 13 7.89E-4 0.07
17 SGTC_1245 0418-0076 3.15 17.2 µM 5.68E-4 ERG2 14 8.28E-4 0.01
18 SGTC_312 4092-0653 3.12 6.8 µM 6.18E-4 ergosterol depletion effects on membrane 45 8.96E-4 0.01
19 SGTC_2342 7990637 3.06 118.9 µM 7.70E-4 RNA processing & uracil transport 19 0.00110 0.14
20 SGTC_2492 derrusnin 3.05 100.0 µM 8.03E-4 13 0.00114 0.00

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.146 1.90E-17 YMR079W SEC14 het Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
0.116 1.56E-11 YIR010W DSN1 het Essential component of the MIND kinetochore complex (Mtw1p Including Nnf1p-Nsl1p-Dsn1p) which joins kinetochore subunits contacting DNA to those contacting microtubules; important for chromosome segregation
0.115 1.98E-11 YPL125W KAP120 hom Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p
0.111 1.23E-10 YDL081C RPP1A hom Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component
0.103 2.42E-9 YJL007C_d YJL007C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.101 3.99E-9 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.098 1.41E-8 YJR046W TAH11 het DNA replication licensing factor, required for pre-replication complex assembly
0.094 4.42E-8 YLR309C IMH1 hom Protein involved in vesicular transport, mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi
0.093 5.97E-8 YJL085W EXO70 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.091 1.12E-7 YDR166C SEC5 het Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
0.091 1.12E-7 YPR055W SEC8 het Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
0.091 1.26E-7 YFL005W SEC4 het Rab family GTPase essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane
0.088 2.95E-7 YLR457C NBP1 het Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication
0.085 7.68E-7 YDR059C UBC5 hom Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication
0.082 1.74E-6 YHR010W RPL27A hom Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication