YLR116W / MSL5

Component of the commitment complex, which defines the first step in the splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR116W / MSL5

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:3.34|P-value:9.85E-4 ID:SGTC_47|Compound:0149-0135|FD-Score:3.54|P-value:5.13E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:4.01|P-value:9.93E-5 ID:SGTC_61|Compound:0978-8327|FD-Score:3.81|P-value:2.00E-4 ID:SGTC_62|Compound:0180-0423|FD-Score:4.48|P-value:1.55E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.98|P-value:8.56E-5 ID:SGTC_353|Compound:1988-1175|FD-Score:-3.27|P-value:9.76E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:3.52|P-value:5.53E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-3.79|P-value:1.71E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.70|P-value:2.98E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:5.50|P-value:1.53E-7 ID:SGTC_534|Compound:k292-0788|FD-Score:-4.10|P-value:5.43E-5 ID:SGTC_541|Compound:4513-1321|FD-Score:3.73|P-value:2.64E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:3.75|P-value:2.50E-4 ID:SGTC_656|Compound:4090-1979|FD-Score:-3.60|P-value:3.28E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:3.35|P-value:9.59E-4 ID:SGTC_917|Compound:harmaline|FD-Score:4.43|P-value:1.88E-5 ID:SGTC_969|Compound:1313-0238|FD-Score:-4.02|P-value:7.45E-5 ID:SGTC_981|Compound:1488-1094|FD-Score:3.70|P-value:2.93E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:-3.47|P-value:5.17E-4 ID:SGTC_1174|Compound:0739-0097|FD-Score:3.45|P-value:6.77E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:5.25|P-value:5.05E-7 ID:SGTC_1253|Compound:0550-0034|FD-Score:4.42|P-value:1.99E-5 ID:SGTC_1370|Compound:2001-0092|FD-Score:-4.38|P-value:1.82E-5 ID:SGTC_1452|Compound:4194-0072|FD-Score:-4.46|P-value:1.28E-5 ID:SGTC_1457|Compound:k015-0032|FD-Score:5.83|P-value:2.83E-8 ID:SGTC_1465|Compound:k213-0056|FD-Score:-3.68|P-value:2.53E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:3.36|P-value:9.26E-4 ID:SGTC_1526|Compound:st051518|FD-Score:5.52|P-value:1.38E-7 ID:SGTC_1573|Compound:oleylamine|FD-Score:3.85|P-value:1.75E-4 ID:SGTC_1752|Compound:st044819|FD-Score:-3.46|P-value:5.41E-4 ID:SGTC_1798|Compound:st045719|FD-Score:-4.20|P-value:3.62E-5 ID:SGTC_1961|Compound:st076550|FD-Score:3.46|P-value:6.71E-4 ID:SGTC_1967|Compound:st070177|FD-Score:-4.05|P-value:6.60E-5 ID:SGTC_2021|Compound:4019055|FD-Score:3.46|P-value:6.74E-4 ID:SGTC_2028|Compound:5153890|FD-Score:3.58|P-value:4.49E-4 ID:SGTC_2106|Compound:5357045|FD-Score:3.36|P-value:9.28E-4 ID:SGTC_2240|Compound:6690706|FD-Score:4.15|P-value:5.79E-5 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:3.43|P-value:7.23E-4 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:4.37|P-value:2.47E-5 ID:SGTC_2592|Compound:piperlongumine|FD-Score:5.76|P-value:4.05E-8 ID:SGTC_2796|Compound:7949634|FD-Score:3.94|P-value:1.29E-4 ID:SGTC_2836|Compound:9010338|FD-Score:5.77|P-value:3.91E-8 ID:SGTC_2960|Compound:9079389|FD-Score:5.76|P-value:4.05E-8 ID:SGTC_2962|Compound:9082311|FD-Score:3.84|P-value:1.83E-4 ID:SGTC_3110|Compound:9122132|FD-Score:3.40|P-value:8.11E-4 ID:SGTC_3194|Compound:9111903|FD-Score:4.26|P-value:3.77E-5 ID:SGTC_3207|Compound:9114163|FD-Score:-3.83|P-value:1.49E-4 ID:SGTC_3212|Compound:9128307|FD-Score:4.18|P-value:5.20E-5 ID:SGTC_3355|Compound:9151058|FD-Score:3.38|P-value:8.61E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:3.34|P-value:9.85E-4 ID:SGTC_47|Compound:0149-0135|FD-Score:3.54|P-value:5.13E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:4.01|P-value:9.93E-5 ID:SGTC_61|Compound:0978-8327|FD-Score:3.81|P-value:2.00E-4 ID:SGTC_62|Compound:0180-0423|FD-Score:4.48|P-value:1.55E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.98|P-value:8.56E-5 ID:SGTC_353|Compound:1988-1175|FD-Score:-3.27|P-value:9.76E-4 ID:SGTC_403|Compound:arphamenine a|FD-Score:3.52|P-value:5.53E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-3.79|P-value:1.71E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.70|P-value:2.98E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:5.50|P-value:1.53E-7 ID:SGTC_534|Compound:k292-0788|FD-Score:-4.10|P-value:5.43E-5 ID:SGTC_541|Compound:4513-1321|FD-Score:3.73|P-value:2.64E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:3.75|P-value:2.50E-4 ID:SGTC_656|Compound:4090-1979|FD-Score:-3.60|P-value:3.28E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:3.35|P-value:9.59E-4 ID:SGTC_917|Compound:harmaline|FD-Score:4.43|P-value:1.88E-5 ID:SGTC_969|Compound:1313-0238|FD-Score:-4.02|P-value:7.45E-5 ID:SGTC_981|Compound:1488-1094|FD-Score:3.70|P-value:2.93E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:-3.47|P-value:5.17E-4 ID:SGTC_1174|Compound:0739-0097|FD-Score:3.45|P-value:6.77E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:5.25|P-value:5.05E-7 ID:SGTC_1253|Compound:0550-0034|FD-Score:4.42|P-value:1.99E-5 ID:SGTC_1370|Compound:2001-0092|FD-Score:-4.38|P-value:1.82E-5 ID:SGTC_1452|Compound:4194-0072|FD-Score:-4.46|P-value:1.28E-5 ID:SGTC_1457|Compound:k015-0032|FD-Score:5.83|P-value:2.83E-8 ID:SGTC_1465|Compound:k213-0056|FD-Score:-3.68|P-value:2.53E-4 ID:SGTC_1505|Compound:k297-0037|FD-Score:3.36|P-value:9.26E-4 ID:SGTC_1526|Compound:st051518|FD-Score:5.52|P-value:1.38E-7 ID:SGTC_1573|Compound:oleylamine|FD-Score:3.85|P-value:1.75E-4 ID:SGTC_1752|Compound:st044819|FD-Score:-3.46|P-value:5.41E-4 ID:SGTC_1798|Compound:st045719|FD-Score:-4.20|P-value:3.62E-5 ID:SGTC_1961|Compound:st076550|FD-Score:3.46|P-value:6.71E-4 ID:SGTC_1967|Compound:st070177|FD-Score:-4.05|P-value:6.60E-5 ID:SGTC_2021|Compound:4019055|FD-Score:3.46|P-value:6.74E-4 ID:SGTC_2028|Compound:5153890|FD-Score:3.58|P-value:4.49E-4 ID:SGTC_2106|Compound:5357045|FD-Score:3.36|P-value:9.28E-4 ID:SGTC_2240|Compound:6690706|FD-Score:4.15|P-value:5.79E-5 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:3.43|P-value:7.23E-4 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:4.37|P-value:2.47E-5 ID:SGTC_2592|Compound:piperlongumine|FD-Score:5.76|P-value:4.05E-8 ID:SGTC_2796|Compound:7949634|FD-Score:3.94|P-value:1.29E-4 ID:SGTC_2836|Compound:9010338|FD-Score:5.77|P-value:3.91E-8 ID:SGTC_2960|Compound:9079389|FD-Score:5.76|P-value:4.05E-8 ID:SGTC_2962|Compound:9082311|FD-Score:3.84|P-value:1.83E-4 ID:SGTC_3110|Compound:9122132|FD-Score:3.40|P-value:8.11E-4 ID:SGTC_3194|Compound:9111903|FD-Score:4.26|P-value:3.77E-5 ID:SGTC_3207|Compound:9114163|FD-Score:-3.83|P-value:1.49E-4 ID:SGTC_3212|Compound:9128307|FD-Score:4.18|P-value:5.20E-5 ID:SGTC_3355|Compound:9151058|FD-Score:3.38|P-value:8.61E-4

Top fitness defect scores for YLR116W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1457 k015-0032 5.83 57.3 µM 2.83E-8 2 2.77E-9 0.98
2 SGTC_2836 9010338 5.77 19.5 µM 3.91E-8 RPP1 & pyrimidine depletion 2 4.00E-9 1.76
3 SGTC_2592 piperlongumine 5.76 83.3 µM 4.05E-8 TSC3-RPN4 1 4.17E-9 0.86
4 SGTC_2960 9079389 5.76 6.0 µM 4.05E-8 1 4.17E-9 1.46
5 SGTC_1526 st051518 5.52 75.0 µM 1.38E-7 2 1.69E-8 0.47
6 SGTC_524 r073-0010 5.50 27.8 µM 1.53E-7 6 1.90E-8 0.47
7 SGTC_1245 0418-0076 5.25 17.2 µM 5.05E-7 ERG2 4 7.44E-8 0.09
8 SGTC_62 0180-0423 4.48 3.2 µM 1.55E-5 60S ribosome export 6 3.70E-6 0.22
9 SGTC_917 harmaline 4.43 728.1 nM 1.88E-5 ergosterol biosynthesis 6 4.61E-6 0.12
10 SGTC_1253 0550-0034 4.42 176.0 µM 1.99E-5 5 4.94E-6 0.03
11 SGTC_2574 2',4'-dihydroxychalcone 4.37 14.6 µM 2.47E-5 7 6.32E-6 0.10
12 SGTC_3194 9111903 4.26 49.5 µM 3.77E-5 3 1.02E-5 0.50
13 SGTC_3212 9128307 4.18 49.5 µM 5.20E-5 9 1.48E-5 0.00
14 SGTC_2240 6690706 4.15 185.9 µM 5.79E-5 5 1.67E-5 0.02
15 SGTC_55 0767-0285 4.01 6.6 µM 9.93E-5 7 3.09E-5 0.32
16 SGTC_2796 7949634 3.94 45.5 µM 1.29E-4 7 4.15E-5 0.02
17 SGTC_1573 oleylamine 3.85 1.1 µM 1.75E-4 5 5.90E-5 0.32
18 SGTC_2962 9082311 3.84 13.2 µM 1.83E-4 13 6.20E-5 0.07
19 SGTC_61 0978-8327 3.81 98.6 µM 2.00E-4 ergosterol biosynthesis 10 6.85E-5 0.21
20 SGTC_566 0958-0068 3.75 16.5 µM 2.50E-4 RSC & ERG11 21 8.85E-5 0.32

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.130 4.10E-14 YPL167C REV3 hom Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
0.095 4.05E-8 YBR189W RPS9B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication
0.090 1.81E-7 YGR227W DIE2 hom Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1
0.081 2.77E-6 YDR356W SPC110 het Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nuclear microtubules to SPB; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner
0.078 5.73E-6 YLR207W HRD3 hom Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events
0.078 6.08E-6 YPL034W_p YPL034W_p hom Putative protein of unknown function; YPL034W is not essential gene
0.078 6.87E-6 YDL221W_d YDL221W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 3' end of essential gene CDC13
0.075 1.46E-5 YHL003C LAG1 hom Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress
0.075 1.50E-5 YPL023C MET12 hom Protein with methylenetetrahydrofolate reductase (MTHFR) activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of MTHFR
0.074 1.70E-5 YGL202W ARO8 hom Aromatic aminotransferase I, expression is regulated by general control of amino acid biosynthesis
0.074 1.83E-5 YGR208W SER2 hom Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
0.074 1.87E-5 YER180C ISC10 hom Protein required for sporulation, transcript is induced 7.5 hours after induction of meiosis, expected to play significant role in the formation of reproductive cells
0.073 2.29E-5 YPR181C SEC23 het GTPase-activating protein, stimulates the GTPase activity of Sar1p; component of the Sec23p-Sec24p heterodimer of the COPII vesicle coat, involved in ER to Golgi transport; substrate of Ubp3/Bre5 complex; ubiquitylated by Ub-ligase Rsp5p; proteasome-mediated degradation of Sec23p is regulated by Cdc48p
0.071 3.60E-5 YGL034C_d YGL034C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.071 3.86E-5 YJR140C HIR3 hom Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein