YLR127C / APC2

Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR127C / APC2

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:7.37|P-value:4.64E-18 ID:SGTC_67|Compound:0335-0847|FD-Score:5.43|P-value:1.30E-10 ID:SGTC_73|Compound:0327-0325|FD-Score:-4.04|P-value:1.30E-6 ID:SGTC_75|Compound:0159-0059|FD-Score:4.22|P-value:4.45E-7 ID:SGTC_142|Compound:0335-0849|FD-Score:3.13|P-value:1.33E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.01|P-value:2.29E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:-3.97|P-value:1.93E-6 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.57|P-value:5.20E-8 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:-3.04|P-value:2.04E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:4.02|P-value:1.39E-6 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:-2.92|P-value:3.48E-4 ID:SGTC_480|Compound:am 92016|FD-Score:-6.11|P-value:5.92E-13 ID:SGTC_482|Compound:fipronil|FD-Score:-2.77|P-value:6.29E-4 ID:SGTC_493|Compound:zm226600|FD-Score:2.80|P-value:5.49E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:2.65|P-value:9.94E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.35|P-value:4.85E-5 ID:SGTC_789|Compound:1309-1135|FD-Score:-3.00|P-value:2.37E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:2.90|P-value:3.62E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.35|P-value:4.71E-5 ID:SGTC_1081|Compound:sertraline|FD-Score:4.02|P-value:1.41E-6 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:2.68|P-value:8.99E-4 ID:SGTC_1086|Compound:cerivastatin|FD-Score:4.26|P-value:3.52E-7 ID:SGTC_1107|Compound:amlodipine|FD-Score:-2.79|P-value:5.77E-4 ID:SGTC_1392|Compound:3455-1037|FD-Score:3.21|P-value:9.42E-5 ID:SGTC_1395|Compound:3486-0190|FD-Score:3.95|P-value:2.04E-6 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:2.89|P-value:3.82E-4 ID:SGTC_1755|Compound:st041960|FD-Score:2.85|P-value:4.40E-4 ID:SGTC_1811|Compound:st050069|FD-Score:2.81|P-value:5.38E-4 ID:SGTC_2014|Compound:4013930|FD-Score:2.84|P-value:4.60E-4 ID:SGTC_2163|Compound:5671233|FD-Score:-2.76|P-value:6.49E-4 ID:SGTC_2238|Compound:6671620|FD-Score:-3.38|P-value:4.26E-5 ID:SGTC_2239|Compound:6685329|FD-Score:3.02|P-value:2.13E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-4.22|P-value:4.45E-7 ID:SGTC_2310|Compound:7668677|FD-Score:2.83|P-value:4.87E-4 ID:SGTC_2341|Compound:7987486|FD-Score:4.31|P-value:2.52E-7 ID:SGTC_2352|Compound:9027995|FD-Score:-3.10|P-value:1.52E-4 ID:SGTC_2353|Compound:9059630|FD-Score:-5.76|P-value:1.02E-11 ID:SGTC_2359|Compound:9039622|FD-Score:2.91|P-value:3.43E-4 ID:SGTC_2360|Compound:9040895|FD-Score:6.73|P-value:2.38E-15 ID:SGTC_2369|Compound:9071472|FD-Score:-6.26|P-value:1.60E-13 ID:SGTC_2777|Compound:climbazole|FD-Score:4.82|P-value:9.80E-9 ID:SGTC_2801|Compound:7907800|FD-Score:-4.73|P-value:1.82E-8 ID:SGTC_2803|Compound:7707263|FD-Score:-2.89|P-value:3.92E-4 ID:SGTC_2852|Compound:9023854|FD-Score:2.71|P-value:7.95E-4 ID:SGTC_3021|Compound:9087658|FD-Score:2.78|P-value:6.04E-4 ID:SGTC_3024|Compound:9090631|FD-Score:2.72|P-value:7.79E-4 ID:SGTC_3310|Compound:9130229|FD-Score:3.01|P-value:2.28E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:7.37|P-value:4.64E-18 ID:SGTC_67|Compound:0335-0847|FD-Score:5.43|P-value:1.30E-10 ID:SGTC_73|Compound:0327-0325|FD-Score:-4.04|P-value:1.30E-6 ID:SGTC_75|Compound:0159-0059|FD-Score:4.22|P-value:4.45E-7 ID:SGTC_142|Compound:0335-0849|FD-Score:3.13|P-value:1.33E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.01|P-value:2.29E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:-3.97|P-value:1.93E-6 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.57|P-value:5.20E-8 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:-3.04|P-value:2.04E-4 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:4.02|P-value:1.39E-6 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:-2.92|P-value:3.48E-4 ID:SGTC_480|Compound:am 92016|FD-Score:-6.11|P-value:5.92E-13 ID:SGTC_482|Compound:fipronil|FD-Score:-2.77|P-value:6.29E-4 ID:SGTC_493|Compound:zm226600|FD-Score:2.80|P-value:5.49E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:2.65|P-value:9.94E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.35|P-value:4.85E-5 ID:SGTC_789|Compound:1309-1135|FD-Score:-3.00|P-value:2.37E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:2.90|P-value:3.62E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:3.35|P-value:4.71E-5 ID:SGTC_1081|Compound:sertraline|FD-Score:4.02|P-value:1.41E-6 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:2.68|P-value:8.99E-4 ID:SGTC_1086|Compound:cerivastatin|FD-Score:4.26|P-value:3.52E-7 ID:SGTC_1107|Compound:amlodipine|FD-Score:-2.79|P-value:5.77E-4 ID:SGTC_1392|Compound:3455-1037|FD-Score:3.21|P-value:9.42E-5 ID:SGTC_1395|Compound:3486-0190|FD-Score:3.95|P-value:2.04E-6 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:2.89|P-value:3.82E-4 ID:SGTC_1755|Compound:st041960|FD-Score:2.85|P-value:4.40E-4 ID:SGTC_1811|Compound:st050069|FD-Score:2.81|P-value:5.38E-4 ID:SGTC_2014|Compound:4013930|FD-Score:2.84|P-value:4.60E-4 ID:SGTC_2163|Compound:5671233|FD-Score:-2.76|P-value:6.49E-4 ID:SGTC_2238|Compound:6671620|FD-Score:-3.38|P-value:4.26E-5 ID:SGTC_2239|Compound:6685329|FD-Score:3.02|P-value:2.13E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-4.22|P-value:4.45E-7 ID:SGTC_2310|Compound:7668677|FD-Score:2.83|P-value:4.87E-4 ID:SGTC_2341|Compound:7987486|FD-Score:4.31|P-value:2.52E-7 ID:SGTC_2352|Compound:9027995|FD-Score:-3.10|P-value:1.52E-4 ID:SGTC_2353|Compound:9059630|FD-Score:-5.76|P-value:1.02E-11 ID:SGTC_2359|Compound:9039622|FD-Score:2.91|P-value:3.43E-4 ID:SGTC_2360|Compound:9040895|FD-Score:6.73|P-value:2.38E-15 ID:SGTC_2369|Compound:9071472|FD-Score:-6.26|P-value:1.60E-13 ID:SGTC_2777|Compound:climbazole|FD-Score:4.82|P-value:9.80E-9 ID:SGTC_2801|Compound:7907800|FD-Score:-4.73|P-value:1.82E-8 ID:SGTC_2803|Compound:7707263|FD-Score:-2.89|P-value:3.92E-4 ID:SGTC_2852|Compound:9023854|FD-Score:2.71|P-value:7.95E-4 ID:SGTC_3021|Compound:9087658|FD-Score:2.78|P-value:6.04E-4 ID:SGTC_3024|Compound:9090631|FD-Score:2.72|P-value:7.79E-4 ID:SGTC_3310|Compound:9130229|FD-Score:3.01|P-value:2.28E-4

Top fitness defect scores for YLR127C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_64 000l-0018 7.37 272.1 µM 4.64E-18 ubiquinone biosynthesis & proteasome 22 8.55E-14 0.10
2 SGTC_2360 9040895 6.73 200.0 µM 2.38E-15 RPP1 & pyrimidine depletion 3 8.71E-12 0.55
3 SGTC_67 0335-0847 5.43 266.0 µM 1.30E-10 ubiquinone biosynthesis & proteasome 12 2.86E-8 0.71
4 SGTC_2777 climbazole 4.82 1.5 µM 9.80E-9 iron homeostasis 1 7.13E-7 0.49
5 SGTC_2341 7987486 4.31 176.7 µM 2.52E-7 4 8.03E-6 0.19
6 SGTC_1086 cerivastatin 4.26 2.2 µM 3.52E-7 azole & statin 3 1.03E-5 0.59
7 SGTC_75 0159-0059 4.22 199.0 µM 4.45E-7 fatty acid desaturase (OLE1) 7 1.23E-5 0.04
8 SGTC_473 2-arachidonoylglycerol 4.02 10.0 µM 1.39E-6 3 2.88E-5 0.03
9 SGTC_1081 sertraline 4.02 6.0 µM 1.41E-6 60S ribosome export 6 2.90E-5 0.13
10 SGTC_1395 3486-0190 3.95 4.3 µM 2.04E-6 2 3.83E-5 0.03
11 SGTC_942 3253-1115 3.35 227.0 µM 4.71E-5 16 4.01E-4 0.01
12 SGTC_769 0302-0231 3.35 76.0 µM 4.85E-5 21 4.10E-4 0.00
13 SGTC_1392 3455-1037 3.21 33.9 µM 9.42E-5 7 6.75E-4 0.03
14 SGTC_142 0335-0849 3.13 62.7 µM 1.33E-4 ubiquinone biosynthesis & proteasome 32 8.75E-4 0.03
15 SGTC_2239 6685329 3.02 187.6 µM 2.13E-4 11 0.00125 0.00
16 SGTC_3310 9130229 3.01 44.1 µM 2.28E-4 Golgi 13 0.00131 0.02
17 SGTC_158 k038-0087 3.01 26.4 µM 2.29E-4 ubiquinone biosynthesis & proteasome 46 0.00132 0.02
18 SGTC_2359 9039622 2.91 200.0 µM 3.43E-4 endomembrane recycling 11 0.00179 0.02
19 SGTC_837 0091-0265 2.90 12.5 µM 3.62E-4 ubiquinone biosynthesis & proteasome 23 0.00186 0.07
20 SGTC_1587 estradiol valerate 2.89 56.1 µM 3.82E-4 11 0.00194 0.08

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.259 1.23E-52 YMR110C HFD1 hom Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
0.231 9.14E-42 YLR199C PBA1 hom Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly
0.230 2.00E-41 YPR146C_d YPR146C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.217 5.72E-37 YBR003W COQ1 hom Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
0.207 6.99E-34 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.204 6.17E-33 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.201 7.00E-32 YJL168C SET2 hom Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
0.198 5.95E-31 YNL111C CYB5 hom Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
0.188 4.05E-28 YDR204W COQ4 hom Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.187 8.69E-28 YNL105W_d RRT16_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription
0.179 1.81E-25 YDL183C YDL183C hom Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene
0.178 2.95E-25 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.173 4.85E-24 YDR148C KGD2 hom Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated
0.173 6.46E-24 YPR024W YME1 hom Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover
0.172 9.94E-24 YLR021W IRC25 hom Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions