YLR128W / DCN1

Scaffold-type E3 ligase required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR128W / DCN1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.77|P-value:1.64E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:3.51|P-value:4.08E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:-4.96|P-value:6.94E-7 ID:SGTC_72|Compound:0744-0168|FD-Score:-4.02|P-value:4.44E-5 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.72|P-value:1.43E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:3.42|P-value:5.55E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:4.81|P-value:2.16E-6 ID:SGTC_270|Compound:tamoxifen|FD-Score:-3.45|P-value:3.68E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:-3.34|P-value:5.36E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:3.43|P-value:5.53E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:3.94|P-value:8.59E-5 ID:SGTC_645|Compound:0845-0943|FD-Score:-3.27|P-value:6.96E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:4.43|P-value:1.18E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:6.16|P-value:1.76E-9 ID:SGTC_1108|Compound:betulinic acid|FD-Score:3.58|P-value:3.21E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:-3.58|P-value:2.31E-4 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.36|P-value:6.83E-4 ID:SGTC_1743|Compound:st037606|FD-Score:4.60|P-value:5.46E-6 ID:SGTC_1746|Compound:st038117|FD-Score:4.53|P-value:7.53E-6 ID:SGTC_1827|Compound:berberine|FD-Score:-3.62|P-value:2.04E-4 ID:SGTC_2088|Compound:5278280|FD-Score:4.43|P-value:1.18E-5 ID:SGTC_2099|Compound:5377090|FD-Score:3.49|P-value:4.45E-4 ID:SGTC_2206|Compound:7254556|FD-Score:3.39|P-value:6.32E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:5.47|P-value:8.37E-8 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.97|P-value:1.02E-6 ID:SGTC_2587|Compound:piperic acid|FD-Score:-3.81|P-value:9.78E-5 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:-7.86|P-value:1.21E-14 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:4.10|P-value:4.60E-5 ID:SGTC_2591|Compound:skatole|FD-Score:4.28|P-value:2.15E-5 ID:SGTC_2600|Compound:tangeritin|FD-Score:-3.30|P-value:6.19E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:-4.68|P-value:2.58E-6 ID:SGTC_2724|Compound:maprotiline|FD-Score:3.97|P-value:7.46E-5 ID:SGTC_2743|Compound:auranofin|FD-Score:-3.56|P-value:2.55E-4 ID:SGTC_2771|Compound:fluoxetine|FD-Score:-3.82|P-value:9.67E-5 ID:SGTC_2835|Compound:9006588|FD-Score:-6.07|P-value:1.81E-9 ID:SGTC_2845|Compound:9003024|FD-Score:-4.13|P-value:2.78E-5 ID:SGTC_2862|Compound:9027634|FD-Score:-3.98|P-value:5.12E-5 ID:SGTC_2884|Compound:9058502|FD-Score:-3.36|P-value:5.12E-4 ID:SGTC_3110|Compound:9122132|FD-Score:3.40|P-value:6.09E-4 ID:SGTC_3309|Compound:9129340|FD-Score:3.34|P-value:7.27E-4 ID:SGTC_3328|Compound:9140624|FD-Score:3.48|P-value:4.54E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.77|P-value:1.64E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:3.51|P-value:4.08E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:-4.96|P-value:6.94E-7 ID:SGTC_72|Compound:0744-0168|FD-Score:-4.02|P-value:4.44E-5 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.72|P-value:1.43E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:3.42|P-value:5.55E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:4.81|P-value:2.16E-6 ID:SGTC_270|Compound:tamoxifen|FD-Score:-3.45|P-value:3.68E-4 ID:SGTC_302|Compound:1094-0063|FD-Score:-3.34|P-value:5.36E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:3.43|P-value:5.53E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:3.94|P-value:8.59E-5 ID:SGTC_645|Compound:0845-0943|FD-Score:-3.27|P-value:6.96E-4 ID:SGTC_667|Compound:1498-1044|FD-Score:4.43|P-value:1.18E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:6.16|P-value:1.76E-9 ID:SGTC_1108|Compound:betulinic acid|FD-Score:3.58|P-value:3.21E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:-3.58|P-value:2.31E-4 ID:SGTC_1293|Compound:1000-1278|FD-Score:3.36|P-value:6.83E-4 ID:SGTC_1743|Compound:st037606|FD-Score:4.60|P-value:5.46E-6 ID:SGTC_1746|Compound:st038117|FD-Score:4.53|P-value:7.53E-6 ID:SGTC_1827|Compound:berberine|FD-Score:-3.62|P-value:2.04E-4 ID:SGTC_2088|Compound:5278280|FD-Score:4.43|P-value:1.18E-5 ID:SGTC_2099|Compound:5377090|FD-Score:3.49|P-value:4.45E-4 ID:SGTC_2206|Compound:7254556|FD-Score:3.39|P-value:6.32E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:5.47|P-value:8.37E-8 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.97|P-value:1.02E-6 ID:SGTC_2587|Compound:piperic acid|FD-Score:-3.81|P-value:9.78E-5 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:-7.86|P-value:1.21E-14 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:4.10|P-value:4.60E-5 ID:SGTC_2591|Compound:skatole|FD-Score:4.28|P-value:2.15E-5 ID:SGTC_2600|Compound:tangeritin|FD-Score:-3.30|P-value:6.19E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:-4.68|P-value:2.58E-6 ID:SGTC_2724|Compound:maprotiline|FD-Score:3.97|P-value:7.46E-5 ID:SGTC_2743|Compound:auranofin|FD-Score:-3.56|P-value:2.55E-4 ID:SGTC_2771|Compound:fluoxetine|FD-Score:-3.82|P-value:9.67E-5 ID:SGTC_2835|Compound:9006588|FD-Score:-6.07|P-value:1.81E-9 ID:SGTC_2845|Compound:9003024|FD-Score:-4.13|P-value:2.78E-5 ID:SGTC_2862|Compound:9027634|FD-Score:-3.98|P-value:5.12E-5 ID:SGTC_2884|Compound:9058502|FD-Score:-3.36|P-value:5.12E-4 ID:SGTC_3110|Compound:9122132|FD-Score:3.40|P-value:6.09E-4 ID:SGTC_3309|Compound:9129340|FD-Score:3.34|P-value:7.27E-4 ID:SGTC_3328|Compound:9140624|FD-Score:3.48|P-value:4.54E-4

Top fitness defect scores for YLR128W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1044 1222-0021 6.16 1.8 µM 1.76E-9 heme biosynthesis & mitochondrial translocase 14 3.54E-10
2 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 5.47 3.7 µM 8.37E-8 20 2.28E-8
3 SGTC_2582 agaric acid 4.97 100.0 µM 1.02E-6 redox potentiating 224 3.40E-7
4 SGTC_158 k038-0087 4.81 26.4 µM 2.16E-6 ubiquinone biosynthesis & proteasome 95 7.66E-7
5 SGTC_1743 st037606 4.60 46.4 µM 5.46E-6 11 2.08E-6
6 SGTC_1746 st038117 4.53 42.0 µM 7.53E-6 20 2.95E-6
7 SGTC_667 1498-1044 4.43 59.0 µM 1.18E-5 RPP1 & pyrimidine depletion 9 4.80E-6
8 SGTC_2088 5278280 4.43 127.4 µM 1.18E-5 13 4.81E-6
9 SGTC_2591 skatole 4.28 100.0 µM 2.15E-5 24 9.17E-6
10 SGTC_2590 3,4',5',6',7'-pentamethoxyflavone 4.10 100.0 µM 4.60E-5 60S ribosome export 20 2.09E-5
11 SGTC_2724 maprotiline 3.97 69.6 µM 7.46E-5 ERG2 17 3.52E-5
12 SGTC_390 dec-rvkr-cmk 3.94 16.7 µM 8.59E-5 calcium & mitochondrial duress 25 4.10E-5
13 SGTC_6 0844-0013 3.77 19.8 µM 1.64E-4 105 8.26E-5
14 SGTC_1108 betulinic acid 3.58 729.1 nM 3.21E-4 21 1.70E-4
15 SGTC_36 0335-0881 3.51 14.2 µM 4.08E-4 ubiquinone biosynthesis & proteasome 144 2.21E-4
16 SGTC_2099 5377090 3.49 177.4 µM 4.45E-4 44 2.43E-4
17 SGTC_3328 9140624 3.48 29.0 µM 4.54E-4 30 2.48E-4
18 SGTC_367 0335-0881 3.43 7.1 µM 5.53E-4 ubiquinone biosynthesis & proteasome 104 3.07E-4
19 SGTC_156 3474-0145 3.42 5.6 µM 5.55E-4 endomembrane recycling 69 3.08E-4
20 SGTC_3110 9122132 3.40 49.5 µM 6.09E-4 redox potentiating 27 3.41E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.163 1.66E-21 YDL018C ERP3 hom Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.146 2.43E-17 YCR033W SNT1 hom Subunit of the Set3C deacetylase complex that interacts directly with the Set3C subunit, Sif2p; putative DNA-binding protein; mutant has increased aneuploidy tolerance
0.126 2.57E-13 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.124 4.73E-13 YCL014W BUD3 hom Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding
0.124 6.78E-13 YDR068W DOS2 hom Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.123 7.67E-13 YML052W SUR7 hom Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants
0.122 1.13E-12 YIR043C YIR043C hom Possible pseudogene in strain S288C; YIR043C and the adjacent ORF, YIR044C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family
0.122 1.36E-12 YJL169W_d YJL169W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2
0.115 2.52E-11 YML110C COQ5 hom 2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase, involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes
0.115 2.72E-11 YGL037C PNC1 hom Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress
0.115 2.88E-11 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.114 3.55E-11 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.113 4.68E-11 YNL106C INP52 hom Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.112 6.86E-11 YGR236C SPG1 hom Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.111 1.23E-10 YIL129C TAO3 het Component of the RAM signaling network that is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p