YLR132C / USB1

Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR132C / USB1

Click on Significant Values for Screen Details ID:SGTC_42|Compound:0133-0086|FD-Score:-3.00|P-value:4.25E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-2.93|P-value:5.48E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.65|P-value:2.92E-5 ID:SGTC_139|Compound:0099-0334|FD-Score:-2.91|P-value:6.12E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:7.41|P-value:1.32E-16 ID:SGTC_170|Compound:4197-0004|FD-Score:-2.97|P-value:4.78E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:2.92|P-value:6.61E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:2.96|P-value:5.63E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.19|P-value:1.97E-4 ID:SGTC_520|Compound:3584-0023|FD-Score:-2.93|P-value:5.51E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:2.96|P-value:5.61E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:2.85|P-value:8.35E-4 ID:SGTC_601|Compound:0986-0029|FD-Score:4.37|P-value:7.17E-7 ID:SGTC_618|Compound:k072-0232|FD-Score:3.64|P-value:3.01E-5 ID:SGTC_672|Compound:1441-0018|FD-Score:2.87|P-value:7.78E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:3.62|P-value:3.23E-5 ID:SGTC_779|Compound:1360-0071|FD-Score:3.33|P-value:1.21E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:-2.80|P-value:9.10E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:3.45|P-value:7.14E-5 ID:SGTC_889|Compound:0919-1524|FD-Score:3.15|P-value:2.58E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:-2.98|P-value:4.52E-4 ID:SGTC_1032|Compound:k061-1018|FD-Score:2.90|P-value:7.02E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.64|P-value:2.97E-5 ID:SGTC_1059|Compound:3448-6867|FD-Score:2.98|P-value:5.12E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:5.28|P-value:2.80E-9 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.31|P-value:1.16E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:3.08|P-value:3.45E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:-4.76|P-value:6.33E-8 ID:SGTC_1383|Compound:3049-0032|FD-Score:3.57|P-value:4.22E-5 ID:SGTC_1564|Compound:D-Arginine|FD-Score:2.99|P-value:4.91E-4 ID:SGTC_1603|Compound:st002045|FD-Score:-2.84|P-value:7.90E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.63|P-value:3.12E-5 ID:SGTC_1641|Compound:st009299|FD-Score:2.95|P-value:5.75E-4 ID:SGTC_1744|Compound:st037937|FD-Score:4.28|P-value:1.20E-6 ID:SGTC_1832|Compound:st054113|FD-Score:2.95|P-value:5.79E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.36|P-value:1.05E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.82|P-value:1.28E-5 ID:SGTC_2154|Compound:5570129|FD-Score:-2.78|P-value:9.70E-4 ID:SGTC_2414|Compound:st077232|FD-Score:-4.23|P-value:1.28E-6 ID:SGTC_2446|Compound:5162541|FD-Score:-2.95|P-value:5.13E-4 ID:SGTC_2448|Compound:5185112|FD-Score:3.28|P-value:1.52E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:2.94|P-value:6.13E-4 ID:SGTC_2798|Compound:7813637|FD-Score:2.82|P-value:9.53E-4 ID:SGTC_2811|Compound:7967822|FD-Score:-4.43|P-value:4.34E-7 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.00|P-value:4.25E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:-2.93|P-value:5.48E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.65|P-value:2.92E-5 ID:SGTC_139|Compound:0099-0334|FD-Score:-2.91|P-value:6.12E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:7.41|P-value:1.32E-16 ID:SGTC_170|Compound:4197-0004|FD-Score:-2.97|P-value:4.78E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:2.92|P-value:6.61E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:2.96|P-value:5.63E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.19|P-value:1.97E-4 ID:SGTC_520|Compound:3584-0023|FD-Score:-2.93|P-value:5.51E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:2.96|P-value:5.61E-4 ID:SGTC_566|Compound:0958-0068|FD-Score:2.85|P-value:8.35E-4 ID:SGTC_601|Compound:0986-0029|FD-Score:4.37|P-value:7.17E-7 ID:SGTC_618|Compound:k072-0232|FD-Score:3.64|P-value:3.01E-5 ID:SGTC_672|Compound:1441-0018|FD-Score:2.87|P-value:7.78E-4 ID:SGTC_774|Compound:k060-0086|FD-Score:3.62|P-value:3.23E-5 ID:SGTC_779|Compound:1360-0071|FD-Score:3.33|P-value:1.21E-4 ID:SGTC_799|Compound:1310-0557|FD-Score:-2.80|P-value:9.10E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:3.45|P-value:7.14E-5 ID:SGTC_889|Compound:0919-1524|FD-Score:3.15|P-value:2.58E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:-2.98|P-value:4.52E-4 ID:SGTC_1032|Compound:k061-1018|FD-Score:2.90|P-value:7.02E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.64|P-value:2.97E-5 ID:SGTC_1059|Compound:3448-6867|FD-Score:2.98|P-value:5.12E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:5.28|P-value:2.80E-9 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.31|P-value:1.16E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:3.08|P-value:3.45E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:-4.76|P-value:6.33E-8 ID:SGTC_1383|Compound:3049-0032|FD-Score:3.57|P-value:4.22E-5 ID:SGTC_1564|Compound:D-Arginine|FD-Score:2.99|P-value:4.91E-4 ID:SGTC_1603|Compound:st002045|FD-Score:-2.84|P-value:7.90E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.63|P-value:3.12E-5 ID:SGTC_1641|Compound:st009299|FD-Score:2.95|P-value:5.75E-4 ID:SGTC_1744|Compound:st037937|FD-Score:4.28|P-value:1.20E-6 ID:SGTC_1832|Compound:st054113|FD-Score:2.95|P-value:5.79E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.36|P-value:1.05E-4 ID:SGTC_2064|Compound:5220758|FD-Score:3.82|P-value:1.28E-5 ID:SGTC_2154|Compound:5570129|FD-Score:-2.78|P-value:9.70E-4 ID:SGTC_2414|Compound:st077232|FD-Score:-4.23|P-value:1.28E-6 ID:SGTC_2446|Compound:5162541|FD-Score:-2.95|P-value:5.13E-4 ID:SGTC_2448|Compound:5185112|FD-Score:3.28|P-value:1.52E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:2.94|P-value:6.13E-4 ID:SGTC_2798|Compound:7813637|FD-Score:2.82|P-value:9.53E-4 ID:SGTC_2811|Compound:7967822|FD-Score:-4.43|P-value:4.34E-7

Top fitness defect scores for YLR132C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_164 4100-3994 7.41 89.6 µM 1.32E-16 1 6.19E-14 1.94
2 SGTC_1239 0337-0584 5.28 35.4 µM 2.80E-9 unfolded protein response 19 6.44E-8 0.24
3 SGTC_601 0986-0029 4.37 62.9 µM 7.17E-7 calcium & mitochondrial duress 14 6.23E-6 0.09
4 SGTC_1744 st037937 4.28 38.9 µM 1.20E-6 5 9.55E-6 0.40
5 SGTC_2064 5220758 3.82 200.0 µM 1.28E-5 9 6.75E-5 0.03
6 SGTC_46 0329-0187a 3.65 2.2 µM 2.92E-5 13 1.33E-4 1.21E-4
7 SGTC_1047 2043-3044 3.64 82.0 µM 2.97E-5 11 1.35E-4 0.11
8 SGTC_618 k072-0232 3.64 33.2 µM 3.01E-5 60S ribosome export 9 1.36E-4 0.13
9 SGTC_1611 st001856 3.63 99.4 µM 3.12E-5 12 1.41E-4 0.14
10 SGTC_774 k060-0086 3.62 136.0 µM 3.23E-5 7 1.45E-4 0.03
11 SGTC_1383 3049-0032 3.57 20.7 µM 4.22E-5 60S ribosome export 13 1.81E-4 0.00
12 SGTC_876 0720-0963 3.45 56.8 µM 7.14E-5 11 2.79E-4 0.08
13 SGTC_2002 4023503 3.36 131.0 µM 1.05E-4 mitochondrial processes 18 3.83E-4 0.01
14 SGTC_779 1360-0071 3.33 233.0 µM 1.21E-4 fatty acid desaturase (OLE1) 10 4.31E-4 0.13
15 SGTC_2448 5185112 3.28 27.2 µM 1.52E-4 10 5.22E-4 0.04
16 SGTC_889 0919-1524 3.15 82.7 µM 2.58E-4 7 8.11E-4 0.08
17 SGTC_1245 0418-0076 3.08 17.2 µM 3.45E-4 ERG2 16 0.00103 0.03
18 SGTC_1564 D-Arginine 2.99 115.0 µM 4.91E-4 17 0.00138 0.05
19 SGTC_1059 3448-6867 2.98 171.0 µM 5.12E-4 17 0.00143 0.03
20 SGTC_547 1120-0019 2.96 610.0 nM 5.61E-4 azole & statin 15 0.00155 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.202 2.70E-32 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.179 1.24E-25 YFL032W_d YFL032W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
0.136 2.06E-15 YNL159C ASI2 hom Integral inner nuclear membrane protein that acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals
0.132 1.43E-14 YML052W SUR7 hom Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants
0.128 9.62E-14 YLL062C MHT1 hom S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio
0.116 1.34E-11 YBR162W-A YSY6 hom Protein whose expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion
0.112 8.92E-11 YKL063C_p YKL063C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi
0.110 1.98E-10 YIL086C_d YIL086C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.103 2.12E-9 YPL119C DBP1 hom Putative ATP-dependent RNA helicase of the DEAD-box protein family; mutants show reduced stability of the 40S ribosomal subunit scanning through 5' untranslated regions of mRNAs; protein abundance increases in response to DNA replication stress
0.103 2.50E-9 YBL075C SSA3 hom ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication
0.102 3.54E-9 YPL052W OAZ1 hom Regulator of ornithine decarboxylase (Spe1p), antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p
0.102 3.48E-9 YHR079C IRE1 hom Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
0.097 1.58E-8 YPR074C TKL1 hom Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication
0.093 6.16E-8 YGL170C SPO74 hom Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation
0.093 6.71E-8 YOL154W ZPS1 hom Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH