YLR135W / SLX4

Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR135W / SLX4

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:3.24|P-value:2.58E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:4.68|P-value:2.66E-7 ID:SGTC_181|Compound:1447-1644|FD-Score:-3.26|P-value:2.31E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:6.35|P-value:5.14E-12 ID:SGTC_306|Compound:1672-3436|FD-Score:3.28|P-value:2.26E-4 ID:SGTC_502|Compound:l-744,832|FD-Score:3.18|P-value:3.32E-4 ID:SGTC_518|Compound:1021-0119|FD-Score:2.96|P-value:7.54E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.22|P-value:2.89E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:4.46|P-value:9.14E-7 ID:SGTC_565|Compound:1418-0001|FD-Score:3.14|P-value:3.81E-4 ID:SGTC_570|Compound:0987-0065|FD-Score:2.93|P-value:8.64E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:-3.53|P-value:7.58E-5 ID:SGTC_583|Compound:1488-1091|FD-Score:-4.13|P-value:4.70E-6 ID:SGTC_615|Compound:k087-0205|FD-Score:2.98|P-value:7.17E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:3.27|P-value:2.35E-4 ID:SGTC_662|Compound:k832-1615|FD-Score:2.93|P-value:8.56E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:3.92|P-value:1.37E-5 ID:SGTC_859|Compound:0439-0102|FD-Score:4.30|P-value:2.05E-6 ID:SGTC_1204|Compound:4049-0315|FD-Score:-3.69|P-value:3.84E-5 ID:SGTC_1281|Compound:0884-0031|FD-Score:-3.74|P-value:2.99E-5 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.01|P-value:6.26E-4 ID:SGTC_1569|Compound:carminic acid|FD-Score:2.97|P-value:7.33E-4 ID:SGTC_1707|Compound:st032323|FD-Score:4.10|P-value:5.77E-6 ID:SGTC_1948|Compound:st076329|FD-Score:3.06|P-value:5.37E-4 ID:SGTC_2052|Compound:5265182|FD-Score:3.63|P-value:5.04E-5 ID:SGTC_2107|Compound:5363541|FD-Score:2.96|P-value:7.56E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:2.95|P-value:8.04E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-3.07|P-value:5.01E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-4.20|P-value:3.26E-6 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.85|P-value:1.85E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:3.24|P-value:2.58E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:4.68|P-value:2.66E-7 ID:SGTC_181|Compound:1447-1644|FD-Score:-3.26|P-value:2.31E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:6.35|P-value:5.14E-12 ID:SGTC_306|Compound:1672-3436|FD-Score:3.28|P-value:2.26E-4 ID:SGTC_502|Compound:l-744,832|FD-Score:3.18|P-value:3.32E-4 ID:SGTC_518|Compound:1021-0119|FD-Score:2.96|P-value:7.54E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.22|P-value:2.89E-4 ID:SGTC_564|Compound:3555-0129|FD-Score:4.46|P-value:9.14E-7 ID:SGTC_565|Compound:1418-0001|FD-Score:3.14|P-value:3.81E-4 ID:SGTC_570|Compound:0987-0065|FD-Score:2.93|P-value:8.64E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:-3.53|P-value:7.58E-5 ID:SGTC_583|Compound:1488-1091|FD-Score:-4.13|P-value:4.70E-6 ID:SGTC_615|Compound:k087-0205|FD-Score:2.98|P-value:7.17E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:3.27|P-value:2.35E-4 ID:SGTC_662|Compound:k832-1615|FD-Score:2.93|P-value:8.56E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:3.92|P-value:1.37E-5 ID:SGTC_859|Compound:0439-0102|FD-Score:4.30|P-value:2.05E-6 ID:SGTC_1204|Compound:4049-0315|FD-Score:-3.69|P-value:3.84E-5 ID:SGTC_1281|Compound:0884-0031|FD-Score:-3.74|P-value:2.99E-5 ID:SGTC_1463|Compound:k081-0032|FD-Score:-3.01|P-value:6.26E-4 ID:SGTC_1569|Compound:carminic acid|FD-Score:2.97|P-value:7.33E-4 ID:SGTC_1707|Compound:st032323|FD-Score:4.10|P-value:5.77E-6 ID:SGTC_1948|Compound:st076329|FD-Score:3.06|P-value:5.37E-4 ID:SGTC_2052|Compound:5265182|FD-Score:3.63|P-value:5.04E-5 ID:SGTC_2107|Compound:5363541|FD-Score:2.96|P-value:7.56E-4 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:2.95|P-value:8.04E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-3.07|P-value:5.01E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-4.20|P-value:3.26E-6 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.85|P-value:1.85E-5

Top fitness defect scores for YLR135W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_268 camptothecin 6.35 39.6 µM 5.14E-12 DNA damage response 25 1.06E-10
2 SGTC_158 k038-0087 4.68 26.4 µM 2.66E-7 ubiquinone biosynthesis & proteasome 101 1.41E-6
3 SGTC_564 3555-0129 4.46 330.0 µM 9.14E-7 3 4.16E-6
4 SGTC_859 0439-0102 4.30 54.6 µM 2.05E-6 redox potentiating 14 8.47E-6
5 SGTC_1707 st032323 4.10 6.3 µM 5.77E-6 DNA damage response 51 2.09E-5
6 SGTC_666 0272-0154 3.92 136.0 µM 1.37E-5 cell wall 56 4.48E-5
7 SGTC_2052 5265182 3.63 7.1 µM 5.04E-5 40 1.40E-4
8 SGTC_306 1672-3436 3.28 11.6 µM 2.26E-4 Golgi 33 5.25E-4
9 SGTC_651 3253-0966 3.27 34.4 µM 2.35E-4 calcium & mitochondrial duress 63 5.42E-4
10 SGTC_64 000l-0018 3.24 272.1 µM 2.58E-4 ubiquinone biosynthesis & proteasome 196 5.89E-4
11 SGTC_537 1334-0398 3.22 209.0 µM 2.89E-4 52 6.50E-4
12 SGTC_502 l-744,832 3.18 89.3 µM 3.32E-4 calcium & mitochondrial duress 64 7.36E-4
13 SGTC_565 1418-0001 3.14 44.5 µM 3.81E-4 58 8.31E-4
14 SGTC_1948 st076329 3.06 66.6 µM 5.37E-4 69 0.00112
15 SGTC_615 k087-0205 2.98 167.0 µM 7.17E-4 49 0.00145
16 SGTC_1569 carminic acid 2.97 40.6 µM 7.33E-4 55 0.00148
17 SGTC_518 1021-0119 2.96 62.6 µM 7.54E-4 ERG2 96 0.00151
18 SGTC_2107 5363541 2.96 170.2 µM 7.56E-4 37 0.00152
19 SGTC_2590 3,4',5',6',7'-pentamethoxyflavone 2.95 100.0 µM 8.04E-4 60S ribosome export 69 0.00160
20 SGTC_662 k832-1615 2.93 104.0 µM 8.56E-4 68 0.00169

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.190 1.13E-28 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.183 1.44E-26 YJL074C SMC3 het Subunit of the multiprotein cohesin complex required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member
0.173 6.42E-24 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.172 1.00E-23 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.172 1.01E-23 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.163 2.06E-21 YMR048W CSM3 hom Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
0.157 5.75E-20 YBL088C TEL1 hom Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)
0.157 6.75E-20 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.157 7.64E-20 YGL175C SAE2 hom Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
0.155 1.57E-19 YDR075W PPH3 hom Catalytic subunit of protein phosphatase PP4 complex; active complex is composed of Pph3p and Psy2p, with Psy4p apparently providing additional substrate specificity in some cases; regulates recovery from the DNA damage checkpoint and also the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes
0.152 8.95E-19 YNR041C COQ2 hom Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis
0.151 1.76E-18 YDR217C RAD9 hom DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate
0.150 2.26E-18 YOL135C MED7 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.149 4.84E-18 YPR064W_d YPR064W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.143 8.70E-17 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2