HIPHOP chemogenomics database

YLR140W_d

Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I

Zygosity: Heterozygous strain

fixedexpanded
Profile for YLR140W / YLR140W

Click on Significant Values for Screen Details

Top fitness defect scores for YLR140W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_63	0099-0242	8.46	26.7 µM	1.06E-16	mitochondrial stress	5	1.38E-17	0.02
2	SGTC_2821	7996764	7.21	71.4 µM	1.24E-12		1	2.78E-13	1.12
3	SGTC_123	0083-0120	5.62	66.7 µM	2.41E-8		4	9.56E-9	0.04
4	SGTC_372	0155-0182	4.88	15.9 µM	1.08E-6	mitochondrial response to ROS	5	5.32E-7	0.95
5	SGTC_2600	tangeritin	4.60	88.0 µM	4.05E-6		1	2.15E-6	0.44
6	SGTC_2816	7982706	4.42	58.4 µM	8.84E-6	azole & statin	5	4.91E-6	0.20
7	SGTC_1788	fluconazole	4.16	20.0 µM	2.69E-5	azole & statin	7	1.59E-5	0.26
8	SGTC_2599	2',3-dihydroxy-4,4',6'-trimethoxychalcone	4.08	100.0 µM	3.68E-5		8	2.22E-5	0.30
9	SGTC_156	3474-0145	4.07	5.6 µM	3.85E-5	endomembrane recycling	4	2.33E-5	0.12
10	SGTC_2766	oltipraz	3.91	83.9 µM	7.27E-5	heme requiring	21	4.55E-5	0.07
11	SGTC_1930	st074634	3.90	34.3 µM	7.52E-5	RNA pol III & RNase P/MRP	14	4.71E-5	0.00
12	SGTC_479	n-linoleoylglycine	3.90	10.0 µM	7.77E-5		1	4.88E-5	0.53
13	SGTC_775	4577-0580	3.88	57.3 µM	8.39E-5		4	5.30E-5	0.10
14	SGTC_70	0141-0289	3.73	43.3 µM	1.45E-4	mitochondrial stress	21	9.46E-5	0.03
15	SGTC_2287	7945887	3.73	168.8 µM	1.46E-4		2	9.49E-5	0.22
16	SGTC_35	0133-0054	3.57	64.1 µM	2.68E-4		15	1.81E-4	0.05
17	SGTC_1906	st060206	3.55	94.7 µM	2.88E-4		4	1.95E-4	0.23
18	SGTC_2026	5148700	3.52	152.0 µM	3.19E-4		8	2.17E-4	0.08
19	SGTC_2103	5378138	3.49	10.0 µM	3.51E-4		3	2.40E-4	0.07
20	SGTC_3181	9108277	3.48	49.5 µM	3.59E-4	iron homeostasis	18	2.46E-4	0.25

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.130	3.54E-14	YBR192W	RIM2	het	Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
0.120	2.79E-12	YGR204W	ADE3	hom	Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase, involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
0.117	8.77E-12	YDR536W	STL1	hom	Glycerol proton symporter of the plasma membrane, subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock
0.109	2.81E-10	YLR359W	ADE13	het	Adenylosuccinate lyase, catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency
0.108	3.78E-10	YLR260W	LCB5	hom	Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules
0.107	6.18E-10	YJL181W_p	YJL181W_p	hom	Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; YJL181W has a paralog, YJR030C, that arose from the whole genome duplication
0.104	1.35E-9	YLR058C	SHM2	hom	Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis
0.103	2.23E-9	YLR123C_d	YLR123C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR122C; contains characteristic aminoacyl-tRNA motif
0.101	4.93E-9	YMR113W	FOL3	het	Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
0.095	3.04E-8	YDR509W_d	YDR509W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.094	5.01E-8	YEL050C	RML2	hom	Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L2 ribosomal protein; fat21 mutant allele causes inability to utilize oleate and may interfere with activity of the Adr1p transcription factor
0.092	9.07E-8	YMR173W	DDR48	hom	DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress
0.091	1.14E-7	YGL129C	RSM23	hom	Mitochondrial ribosomal protein of the small subunit, has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p
0.090	1.63E-7	YMR300C	ADE4	hom	Phosphoribosylpyrophosphate amidotransferase (PRPPAT; amidophosphoribosyltransferase), catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway
0.090	2.01E-7	YKL195W	MIA40	het	Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p