YLR143W / DPH6

Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR143W / DPH6

Click on Significant Values for Screen Details

Top fitness defect scores for YLR143W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_377 3970-0964 7.02 33.1 µM 1.68E-10 9 1.12E-12
2 SGTC_1745 st081495 6.00 74.0 µM 4.02E-8 RNA processing & uracil transport 27 9.97E-10
3 SGTC_445 e6 berbamine 5.34 66.0 µM 9.00E-7 NEO1-PIK1 47 4.68E-8
4 SGTC_2832 7992762 5.31 52.0 µM 1.01E-6 10 5.38E-8
5 SGTC_1750 st081859 5.17 2.4 µM 1.87E-6 endomembrane recycling 132 1.16E-7
6 SGTC_3134 9127454 5.09 49.5 µM 2.61E-6 6 1.75E-7
7 SGTC_2485 5633444 5.03 17.8 µM 3.44E-6 redox potentiating 23 2.46E-7
8 SGTC_221 trifluoperazine 5.02 10.3 µM 3.59E-6 NEO1-PIK1 161 2.60E-7
9 SGTC_759 3486-0358 4.77 89.3 µM 1.01E-5 13 9.27E-7
10 SGTC_760 0568-0725 4.73 82.9 µM 1.17E-5 3 1.12E-6
11 SGTC_397 arbidol 4.72 50.0 µM 1.24E-5 cell wall 11 1.20E-6
12 SGTC_2752 diclazuril 4.55 33.1 µM 2.36E-5 90 2.66E-6
13 SGTC_923 1900-2434 4.41 73.3 µM 4.06E-5 RNA processing & uracil transport 22 5.20E-6
14 SGTC_1537 2',6'-dihydroxyflavanone 4.05 78.0 µM 1.48E-4 16 2.56E-5
15 SGTC_380 3970-0968 4.04 6.0 µM 1.51E-4 12 2.64E-5
16 SGTC_1934 st074720 3.94 32.6 µM 2.12E-4 57 4.00E-5
17 SGTC_2464 5717415 3.89 7.7 µM 2.53E-4 17 4.97E-5
18 SGTC_422 cantharidin 3.75 155.0 µM 4.01E-4 cell wall signaling 65 8.76E-5
19 SGTC_1193 1488-0126 3.62 41.5 µM 6.20E-4 34 1.50E-4
20 SGTC_2465 5809242 3.59 33.2 µM 6.72E-4 5 1.66E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.192 4.20E-29 YPR029C APL4 hom Gamma-adaptin, large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
0.170 4.18E-23 YKL191W DPH2 hom Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p
0.160 1.18E-20 YLR169W_d YLR169W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.142 1.66E-16 YBR246W RRT2 hom Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.138 1.13E-15 YER120W SCS2 hom Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication
0.138 1.21E-15 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.130 4.33E-14 YBR245C ISW1 hom ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions
0.128 1.04E-13 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.128 1.20E-13 YNL295W_p YNL295W_p hom Putative protein of unknown function
0.127 1.55E-13 YGR202C PCT1 hom Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
0.118 8.63E-12 YDR159W SAC3 hom Nuclear pore-associated protein; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP)
0.114 3.41E-11 YOL001W PHO80 hom Cyclin, interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling
0.113 6.40E-11 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.112 7.67E-11 YEL019C MMS21 het SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination
0.109 2.65E-10 YBR194W AIM4 hom Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress