YLR144C / ACF2

Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR144C / ACF2

Click on Significant Values for Screen Details ID:SGTC_86|Compound:0417-1630|FD-Score:3.34|P-value:3.69E-4 ID:SGTC_105|Compound:0111-0055|FD-Score:3.31|P-value:4.07E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:3.48|P-value:2.19E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:4.72|P-value:8.91E-7 ID:SGTC_161|Compound:4065-0411|FD-Score:-4.52|P-value:2.61E-6 ID:SGTC_164|Compound:4100-3994|FD-Score:-4.42|P-value:4.12E-6 ID:SGTC_165|Compound:k038-0023|FD-Score:4.95|P-value:2.82E-7 ID:SGTC_174|Compound:3553-0389|FD-Score:3.39|P-value:3.07E-4 ID:SGTC_228|Compound:caffeine|FD-Score:3.37|P-value:3.26E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:-5.54|P-value:1.19E-8 ID:SGTC_608|Compound:k048-0125|FD-Score:-3.22|P-value:5.90E-4 ID:SGTC_617|Compound:3770-0098|FD-Score:4.66|P-value:1.26E-6 ID:SGTC_810|Compound:3825-7150|FD-Score:3.12|P-value:8.03E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:-4.96|P-value:2.84E-7 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:-3.18|P-value:6.83E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:3.51|P-value:1.91E-4 ID:SGTC_1508|Compound:1080-0625|FD-Score:-4.01|P-value:2.62E-5 ID:SGTC_1510|Compound:1123-0200|FD-Score:-4.92|P-value:3.54E-7 ID:SGTC_1634|Compound:st006576|FD-Score:3.37|P-value:3.31E-4 ID:SGTC_1732|Compound:st034308|FD-Score:3.09|P-value:8.96E-4 ID:SGTC_1829|Compound:berberine|FD-Score:4.59|P-value:1.78E-6 ID:SGTC_1993|Compound:st073995|FD-Score:3.09|P-value:8.91E-4 ID:SGTC_2018|Compound:5133068|FD-Score:3.16|P-value:6.91E-4 ID:SGTC_2027|Compound:5150920|FD-Score:-3.78|P-value:6.95E-5 ID:SGTC_2159|Compound:5658611|FD-Score:3.21|P-value:5.73E-4 ID:SGTC_2292|Compound:7671323|FD-Score:-3.33|P-value:4.03E-4 ID:SGTC_2299|Compound:7976368|FD-Score:-3.95|P-value:3.39E-5 ID:SGTC_2484|Compound:5809818|FD-Score:3.82|P-value:5.64E-5 ID:SGTC_2532|Compound:xanthone|FD-Score:-3.45|P-value:2.51E-4 ID:SGTC_2555|Compound:cholic acid|FD-Score:-5.31|P-value:4.42E-8 ID:SGTC_2584|Compound:androsterone|FD-Score:-3.63|P-value:1.27E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:4.03|P-value:2.32E-5 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-3.23|P-value:5.66E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:5.32|P-value:3.83E-8 ID:SGTC_2878|Compound:9043973|FD-Score:3.77|P-value:6.77E-5 ID:SGTC_3052|Compound:9092717|FD-Score:-3.09|P-value:9.39E-4 ID:SGTC_3095|Compound:9117207|FD-Score:-3.23|P-value:5.65E-4 ID:SGTC_3096|Compound:9117468|FD-Score:-3.54|P-value:1.78E-4 ID:SGTC_3309|Compound:9129340|FD-Score:-3.71|P-value:9.37E-5 ID:SGTC_3335|Compound:9143315|FD-Score:3.51|P-value:1.94E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:3.34|P-value:3.69E-4 ID:SGTC_105|Compound:0111-0055|FD-Score:3.31|P-value:4.07E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:3.48|P-value:2.19E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:4.72|P-value:8.91E-7 ID:SGTC_161|Compound:4065-0411|FD-Score:-4.52|P-value:2.61E-6 ID:SGTC_164|Compound:4100-3994|FD-Score:-4.42|P-value:4.12E-6 ID:SGTC_165|Compound:k038-0023|FD-Score:4.95|P-value:2.82E-7 ID:SGTC_174|Compound:3553-0389|FD-Score:3.39|P-value:3.07E-4 ID:SGTC_228|Compound:caffeine|FD-Score:3.37|P-value:3.26E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:-5.54|P-value:1.19E-8 ID:SGTC_608|Compound:k048-0125|FD-Score:-3.22|P-value:5.90E-4 ID:SGTC_617|Compound:3770-0098|FD-Score:4.66|P-value:1.26E-6 ID:SGTC_810|Compound:3825-7150|FD-Score:3.12|P-value:8.03E-4 ID:SGTC_1047|Compound:2043-3044|FD-Score:-4.96|P-value:2.84E-7 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:-3.18|P-value:6.83E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:3.51|P-value:1.91E-4 ID:SGTC_1508|Compound:1080-0625|FD-Score:-4.01|P-value:2.62E-5 ID:SGTC_1510|Compound:1123-0200|FD-Score:-4.92|P-value:3.54E-7 ID:SGTC_1634|Compound:st006576|FD-Score:3.37|P-value:3.31E-4 ID:SGTC_1732|Compound:st034308|FD-Score:3.09|P-value:8.96E-4 ID:SGTC_1829|Compound:berberine|FD-Score:4.59|P-value:1.78E-6 ID:SGTC_1993|Compound:st073995|FD-Score:3.09|P-value:8.91E-4 ID:SGTC_2018|Compound:5133068|FD-Score:3.16|P-value:6.91E-4 ID:SGTC_2027|Compound:5150920|FD-Score:-3.78|P-value:6.95E-5 ID:SGTC_2159|Compound:5658611|FD-Score:3.21|P-value:5.73E-4 ID:SGTC_2292|Compound:7671323|FD-Score:-3.33|P-value:4.03E-4 ID:SGTC_2299|Compound:7976368|FD-Score:-3.95|P-value:3.39E-5 ID:SGTC_2484|Compound:5809818|FD-Score:3.82|P-value:5.64E-5 ID:SGTC_2532|Compound:xanthone|FD-Score:-3.45|P-value:2.51E-4 ID:SGTC_2555|Compound:cholic acid|FD-Score:-5.31|P-value:4.42E-8 ID:SGTC_2584|Compound:androsterone|FD-Score:-3.63|P-value:1.27E-4 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:4.03|P-value:2.32E-5 ID:SGTC_2753|Compound:fluvastatin|FD-Score:-3.23|P-value:5.66E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:5.32|P-value:3.83E-8 ID:SGTC_2878|Compound:9043973|FD-Score:3.77|P-value:6.77E-5 ID:SGTC_3052|Compound:9092717|FD-Score:-3.09|P-value:9.39E-4 ID:SGTC_3095|Compound:9117207|FD-Score:-3.23|P-value:5.65E-4 ID:SGTC_3096|Compound:9117468|FD-Score:-3.54|P-value:1.78E-4 ID:SGTC_3309|Compound:9129340|FD-Score:-3.71|P-value:9.37E-5 ID:SGTC_3335|Compound:9143315|FD-Score:3.51|P-value:1.94E-4

Top fitness defect scores for YLR144C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2756 carbadox 5.32 75.1 µM 3.83E-8 28 5.26E-8
2 SGTC_165 k038-0023 4.95 64.4 µM 2.82E-7 ubiquinone biosynthesis & proteasome 44 3.72E-7
3 SGTC_137 0438-0332 4.72 85.4 µM 8.91E-7 ubiquinone biosynthesis & proteasome 87 1.15E-6
4 SGTC_617 3770-0098 4.66 35.0 µM 1.26E-6 exosome 8 1.61E-6
5 SGTC_1829 berberine 4.59 14.4 µM 1.78E-6 60S ribosome export 10 2.27E-6
6 SGTC_2609 2,3,4-trihydroxy-4'-ethoxybenzophenone 4.03 70.0 µM 2.32E-5 redox potentiating 69 2.82E-5
7 SGTC_2484 5809818 3.82 12.0 µM 5.64E-5 19 6.73E-5
8 SGTC_2878 9043973 3.77 26.0 µM 6.77E-5 25 8.05E-5
9 SGTC_1213 1315-0166 3.51 115.0 µM 1.91E-4 43 2.22E-4
10 SGTC_3335 9143315 3.51 29.6 µM 1.94E-4 fatty acid desaturase (OLE1) 22 2.26E-4
11 SGTC_129 0088-0017 3.48 176.6 µM 2.19E-4 81 2.55E-4
12 SGTC_174 3553-0389 3.39 74.3 µM 3.07E-4 39 3.55E-4
13 SGTC_228 caffeine 3.37 993.1 µM 3.26E-4 21 3.76E-4
14 SGTC_1634 st006576 3.37 41.0 µM 3.31E-4 TSC3-RPN4 38 3.82E-4
15 SGTC_86 0417-1630 3.34 471.9 µM 3.69E-4 ubiquinone biosynthesis & proteasome 191 4.24E-4
16 SGTC_105 0111-0055 3.31 734.8 µM 4.07E-4 19 4.68E-4
17 SGTC_2159 5658611 3.21 73.8 µM 5.73E-4 20 6.54E-4
18 SGTC_2018 5133068 3.16 143.0 µM 6.91E-4 60 7.86E-4
19 SGTC_810 3825-7150 3.12 143.0 µM 8.03E-4 60S ribosome export 31 9.10E-4
20 SGTC_1993 st073995 3.09 70.8 µM 8.91E-4 48 0.00101

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.162 3.02E-21 YML034C-A_d YML034C-A_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.143 9.31E-17 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.126 2.63E-13 YLR202C_d YLR202C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron
0.123 8.21E-13 YJL168C SET2 hom Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
0.117 1.21E-11 YBR003W COQ1 hom Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
0.114 3.53E-11 YOR165W SEY1 hom Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3
0.109 2.47E-10 YDL183C YDL183C hom Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene
0.104 1.81E-9 YDR086C SSS1 het Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
0.103 1.92E-9 YJR043C POL32 hom Third subunit of DNA polymerase delta, involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; interacts with Hys2p, PCNA (Pol30p), and Pol1p
0.100 6.41E-9 YJL193W_p YJL193W_p hom Putative protein of unknown function, predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
0.095 3.38E-8 YKR071C DRE2 het Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18pin an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress
0.095 3.75E-8 YER019C-A SBH2 hom Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p
0.094 4.50E-8 YPR065W ROX1 hom Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance
0.094 4.97E-8 YJL155C FBP26 hom Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress
0.094 5.11E-8 YOL008W COQ10 hom Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes