YLR148W / PEP3

Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR148W / PEP3

Click on Significant Values for Screen Details ID:SGTC_31|Compound:0508-1974|FD-Score:4.55|P-value:3.43E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:-4.59|P-value:2.75E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-6.30|P-value:1.05E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.19|P-value:4.61E-5 ID:SGTC_70|Compound:0141-0289|FD-Score:4.38|P-value:5.42E-4 ID:SGTC_193|Compound:1273-0060|FD-Score:-6.17|P-value:1.71E-6 ID:SGTC_201|Compound:4262-0159|FD-Score:-5.55|P-value:1.46E-5 ID:SGTC_206|Compound:4546-0025|FD-Score:6.39|P-value:8.99E-7 ID:SGTC_299|Compound:1273-0059|FD-Score:-5.52|P-value:1.63E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:7.19|P-value:3.78E-8 ID:SGTC_310|Compound:1273-0058|FD-Score:-8.07|P-value:6.39E-10 ID:SGTC_312|Compound:4092-0653|FD-Score:-5.39|P-value:2.47E-5 ID:SGTC_313|Compound:3474-0040|FD-Score:4.64|P-value:2.68E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:-4.11|P-value:9.69E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:-5.21|P-value:4.33E-5 ID:SGTC_443|Compound:clozapine|FD-Score:4.75|P-value:1.95E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:4.19|P-value:8.90E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.75|P-value:1.93E-7 ID:SGTC_568|Compound:1193-0097|FD-Score:-4.14|P-value:8.93E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:4.97|P-value:1.03E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:-4.60|P-value:2.65E-4 ID:SGTC_689|Compound:0833-0039|FD-Score:-4.92|P-value:1.05E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:4.65|P-value:2.57E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.50|P-value:3.94E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:4.65|P-value:2.55E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:4.78|P-value:1.78E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:5.20|P-value:5.12E-5 ID:SGTC_893|Compound:0958-0369|FD-Score:-5.10|P-value:6.15E-5 ID:SGTC_897|Compound:1000-0139|FD-Score:4.43|P-value:4.73E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:6.99|P-value:8.58E-8 ID:SGTC_958|Compound:1222-0007|FD-Score:6.87|P-value:1.38E-7 ID:SGTC_974|Compound:1326-0144|FD-Score:5.24|P-value:4.53E-5 ID:SGTC_1010|Compound:4112-3296|FD-Score:4.44|P-value:4.53E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:6.62|P-value:3.75E-7 ID:SGTC_1130|Compound:3454-2858|FD-Score:10.30|P-value:6.35E-15 ID:SGTC_1132|Compound:3456-3614|FD-Score:5.05|P-value:8.06E-5 ID:SGTC_1277|Compound:0873-0036|FD-Score:5.51|P-value:1.93E-5 ID:SGTC_1464|Compound:k208-0027|FD-Score:5.65|P-value:1.21E-5 ID:SGTC_1474|Compound:0330-0125|FD-Score:4.25|P-value:7.56E-4 ID:SGTC_1547|Compound:harmine|FD-Score:5.38|P-value:2.90E-5 ID:SGTC_1581|Compound:himbacine|FD-Score:5.97|P-value:4.11E-6 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:4.25|P-value:7.58E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:7.88|P-value:1.87E-9 ID:SGTC_1629|Compound:st005085|FD-Score:8.02|P-value:9.66E-10 ID:SGTC_1754|Compound:st042065|FD-Score:4.56|P-value:3.26E-4 ID:SGTC_1760|Compound:st045453|FD-Score:6.84|P-value:1.58E-7 ID:SGTC_1781|Compound:st038725|FD-Score:5.34|P-value:3.28E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.35|P-value:5.26E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:6.42|P-value:7.93E-7 ID:SGTC_1877|Compound:wortmannin|FD-Score:6.54|P-value:4.96E-7 ID:SGTC_1991|Compound:st073000|FD-Score:5.28|P-value:4.01E-5 ID:SGTC_1993|Compound:st073995|FD-Score:4.28|P-value:7.08E-4 ID:SGTC_2038|Compound:4100038|FD-Score:-4.65|P-value:2.31E-4 ID:SGTC_2077|Compound:5212015|FD-Score:4.51|P-value:3.77E-4 ID:SGTC_2086|Compound:5274770|FD-Score:7.13|P-value:4.90E-8 ID:SGTC_2092|Compound:5303694|FD-Score:5.61|P-value:1.37E-5 ID:SGTC_2166|Compound:5933061|FD-Score:-4.21|P-value:7.51E-4 ID:SGTC_2187|Compound:6125751|FD-Score:4.35|P-value:5.81E-4 ID:SGTC_2242|Compound:6630449|FD-Score:4.63|P-value:2.74E-4 ID:SGTC_2254|Compound:7938975|FD-Score:4.33|P-value:6.15E-4 ID:SGTC_2257|Compound:7960413|FD-Score:4.87|P-value:1.39E-4 ID:SGTC_2372|Compound:9071633|FD-Score:5.10|P-value:7.02E-5 ID:SGTC_2430|Compound:5571206|FD-Score:9.41|P-value:9.28E-13 ID:SGTC_2433|Compound:5607874|FD-Score:4.24|P-value:7.83E-4 ID:SGTC_2434|Compound:5670830|FD-Score:8.88|P-value:1.50E-11 ID:SGTC_2436|Compound:5633522|FD-Score:-5.49|P-value:1.81E-5 ID:SGTC_2481|Compound:5478948|FD-Score:4.73|P-value:2.09E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:4.36|P-value:5.71E-4 ID:SGTC_2526|Compound:patulin|FD-Score:5.29|P-value:3.84E-5 ID:SGTC_2544|Compound:sphondin|FD-Score:4.63|P-value:2.71E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:7.38|P-value:1.71E-8 ID:SGTC_2607|Compound:solasodine|FD-Score:-4.26|P-value:6.63E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:6.29|P-value:1.30E-6 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:4.29|P-value:6.84E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:5.96|P-value:4.20E-6 ID:SGTC_2743|Compound:auranofin|FD-Score:4.49|P-value:3.97E-4 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:6.18|P-value:1.93E-6 ID:SGTC_2777|Compound:climbazole|FD-Score:-4.99|P-value:8.58E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:-4.49|P-value:3.63E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:5.38|P-value:2.91E-5 ID:SGTC_2937|Compound:9044753|FD-Score:4.89|P-value:1.32E-4 ID:SGTC_3081|Compound:9117243|FD-Score:-6.52|P-value:4.74E-7 ID:SGTC_3085|Compound:9117376|FD-Score:4.98|P-value:9.88E-5 ID:SGTC_3126|Compound:9127922|FD-Score:-5.55|P-value:1.49E-5 ID:SGTC_3266|Compound:9136860|FD-Score:-6.61|P-value:3.31E-7 ID:SGTC_3317|Compound:9138495|FD-Score:4.36|P-value:5.71E-4 ID:SGTC_31|Compound:0508-1974|FD-Score:4.55|P-value:3.43E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:-4.59|P-value:2.75E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-6.30|P-value:1.05E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.19|P-value:4.61E-5 ID:SGTC_70|Compound:0141-0289|FD-Score:4.38|P-value:5.42E-4 ID:SGTC_193|Compound:1273-0060|FD-Score:-6.17|P-value:1.71E-6 ID:SGTC_201|Compound:4262-0159|FD-Score:-5.55|P-value:1.46E-5 ID:SGTC_206|Compound:4546-0025|FD-Score:6.39|P-value:8.99E-7 ID:SGTC_299|Compound:1273-0059|FD-Score:-5.52|P-value:1.63E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:7.19|P-value:3.78E-8 ID:SGTC_310|Compound:1273-0058|FD-Score:-8.07|P-value:6.39E-10 ID:SGTC_312|Compound:4092-0653|FD-Score:-5.39|P-value:2.47E-5 ID:SGTC_313|Compound:3474-0040|FD-Score:4.64|P-value:2.68E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:-4.11|P-value:9.69E-4 ID:SGTC_320|Compound:4092-0846|FD-Score:-5.21|P-value:4.33E-5 ID:SGTC_443|Compound:clozapine|FD-Score:4.75|P-value:1.95E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:4.19|P-value:8.90E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.75|P-value:1.93E-7 ID:SGTC_568|Compound:1193-0097|FD-Score:-4.14|P-value:8.93E-4 ID:SGTC_577|Compound:r071-0003|FD-Score:4.97|P-value:1.03E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:-4.60|P-value:2.65E-4 ID:SGTC_689|Compound:0833-0039|FD-Score:-4.92|P-value:1.05E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:4.65|P-value:2.57E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.50|P-value:3.94E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:4.65|P-value:2.55E-4 ID:SGTC_886|Compound:0866-0150|FD-Score:4.78|P-value:1.78E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:5.20|P-value:5.12E-5 ID:SGTC_893|Compound:0958-0369|FD-Score:-5.10|P-value:6.15E-5 ID:SGTC_897|Compound:1000-0139|FD-Score:4.43|P-value:4.73E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:6.99|P-value:8.58E-8 ID:SGTC_958|Compound:1222-0007|FD-Score:6.87|P-value:1.38E-7 ID:SGTC_974|Compound:1326-0144|FD-Score:5.24|P-value:4.53E-5 ID:SGTC_1010|Compound:4112-3296|FD-Score:4.44|P-value:4.53E-4 ID:SGTC_1036|Compound:k216-0215|FD-Score:6.62|P-value:3.75E-7 ID:SGTC_1130|Compound:3454-2858|FD-Score:10.30|P-value:6.35E-15 ID:SGTC_1132|Compound:3456-3614|FD-Score:5.05|P-value:8.06E-5 ID:SGTC_1277|Compound:0873-0036|FD-Score:5.51|P-value:1.93E-5 ID:SGTC_1464|Compound:k208-0027|FD-Score:5.65|P-value:1.21E-5 ID:SGTC_1474|Compound:0330-0125|FD-Score:4.25|P-value:7.56E-4 ID:SGTC_1547|Compound:harmine|FD-Score:5.38|P-value:2.90E-5 ID:SGTC_1581|Compound:himbacine|FD-Score:5.97|P-value:4.11E-6 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:4.25|P-value:7.58E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:7.88|P-value:1.87E-9 ID:SGTC_1629|Compound:st005085|FD-Score:8.02|P-value:9.66E-10 ID:SGTC_1754|Compound:st042065|FD-Score:4.56|P-value:3.26E-4 ID:SGTC_1760|Compound:st045453|FD-Score:6.84|P-value:1.58E-7 ID:SGTC_1781|Compound:st038725|FD-Score:5.34|P-value:3.28E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.35|P-value:5.26E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:6.42|P-value:7.93E-7 ID:SGTC_1877|Compound:wortmannin|FD-Score:6.54|P-value:4.96E-7 ID:SGTC_1991|Compound:st073000|FD-Score:5.28|P-value:4.01E-5 ID:SGTC_1993|Compound:st073995|FD-Score:4.28|P-value:7.08E-4 ID:SGTC_2038|Compound:4100038|FD-Score:-4.65|P-value:2.31E-4 ID:SGTC_2077|Compound:5212015|FD-Score:4.51|P-value:3.77E-4 ID:SGTC_2086|Compound:5274770|FD-Score:7.13|P-value:4.90E-8 ID:SGTC_2092|Compound:5303694|FD-Score:5.61|P-value:1.37E-5 ID:SGTC_2166|Compound:5933061|FD-Score:-4.21|P-value:7.51E-4 ID:SGTC_2187|Compound:6125751|FD-Score:4.35|P-value:5.81E-4 ID:SGTC_2242|Compound:6630449|FD-Score:4.63|P-value:2.74E-4 ID:SGTC_2254|Compound:7938975|FD-Score:4.33|P-value:6.15E-4 ID:SGTC_2257|Compound:7960413|FD-Score:4.87|P-value:1.39E-4 ID:SGTC_2372|Compound:9071633|FD-Score:5.10|P-value:7.02E-5 ID:SGTC_2430|Compound:5571206|FD-Score:9.41|P-value:9.28E-13 ID:SGTC_2433|Compound:5607874|FD-Score:4.24|P-value:7.83E-4 ID:SGTC_2434|Compound:5670830|FD-Score:8.88|P-value:1.50E-11 ID:SGTC_2436|Compound:5633522|FD-Score:-5.49|P-value:1.81E-5 ID:SGTC_2481|Compound:5478948|FD-Score:4.73|P-value:2.09E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:4.36|P-value:5.71E-4 ID:SGTC_2526|Compound:patulin|FD-Score:5.29|P-value:3.84E-5 ID:SGTC_2544|Compound:sphondin|FD-Score:4.63|P-value:2.71E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:7.38|P-value:1.71E-8 ID:SGTC_2607|Compound:solasodine|FD-Score:-4.26|P-value:6.63E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:6.29|P-value:1.30E-6 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:4.29|P-value:6.84E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:5.96|P-value:4.20E-6 ID:SGTC_2743|Compound:auranofin|FD-Score:4.49|P-value:3.97E-4 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:6.18|P-value:1.93E-6 ID:SGTC_2777|Compound:climbazole|FD-Score:-4.99|P-value:8.58E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:-4.49|P-value:3.63E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:5.38|P-value:2.91E-5 ID:SGTC_2937|Compound:9044753|FD-Score:4.89|P-value:1.32E-4 ID:SGTC_3081|Compound:9117243|FD-Score:-6.52|P-value:4.74E-7 ID:SGTC_3085|Compound:9117376|FD-Score:4.98|P-value:9.88E-5 ID:SGTC_3126|Compound:9127922|FD-Score:-5.55|P-value:1.49E-5 ID:SGTC_3266|Compound:9136860|FD-Score:-6.61|P-value:3.31E-7 ID:SGTC_3317|Compound:9138495|FD-Score:4.36|P-value:5.71E-4

Top fitness defect scores for YLR148W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1130 3454-2858 10.30 15.9 µM 6.35E-15 NEO1 13 3.59E-25
2 SGTC_2430 5571206 9.41 200.0 µM 9.28E-13 10 2.41E-21
3 SGTC_2434 5670830 8.88 169.9 µM 1.50E-11 1 3.29E-19
4 SGTC_1629 st005085 8.02 44.9 µM 9.66E-10 RPP1 & pyrimidine depletion 3 5.09E-16
5 SGTC_1601 4'-hydroxyflavanone 7.88 55.5 µM 1.87E-9 RPP1 & pyrimidine depletion 3 1.64E-15
6 SGTC_2552 juarezic acid 7.38 87.0 µM 1.71E-8 TSC3-RPN4 2 8.02E-14
7 SGTC_301 4119-0100 7.19 24.5 µM 3.78E-8 NEO1 29 3.24E-13
8 SGTC_2086 5274770 7.13 176.6 µM 4.90E-8 3 5.12E-13
9 SGTC_954 1189-0853 6.99 2.9 µM 8.58E-8 endomembrane recycling 8 1.37E-12
10 SGTC_958 1222-0007 6.87 33.0 µM 1.38E-7 5 3.17E-12
11 SGTC_1760 st045453 6.84 10.4 µM 1.58E-7 NEO1-PIK1 69 3.99E-12
12 SGTC_1036 k216-0215 6.62 33.5 µM 3.75E-7 mitochondrial response to ROS 11 1.83E-11
13 SGTC_1877 wortmannin 6.54 25.0 µM 4.96E-7 40 2.99E-11
14 SGTC_1873 rapamycin 6.42 2.0 nM 7.93E-7 27 6.80E-11
15 SGTC_206 4546-0025 6.39 72.0 µM 8.99E-7 NEO1 29 8.48E-11
16 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 6.29 100.0 µM 1.30E-6 8 1.62E-10
17 SGTC_2775 cyproheptadine 6.18 69.6 µM 1.93E-6 3 3.25E-10
18 SGTC_1581 himbacine 5.97 57.9 µM 4.11E-6 Golgi 18 1.22E-9
19 SGTC_2737 bleomycin 5.96 30.5 nM 4.20E-6 Golgi 34 1.27E-9
20 SGTC_1464 k208-0027 5.65 110.0 µM 1.21E-5 10 8.01E-9

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.291 2.53E-66 YGL095C VPS45 hom Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
0.284 3.16E-63 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.262 1.45E-53 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.220 6.60E-38 YLR396C VPS33 hom ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole
0.215 3.50E-36 YKR001C VPS1 hom Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis
0.213 7.62E-36 YDR455C_d YDR455C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
0.212 2.55E-35 YDR129C SAC6 hom Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
0.199 3.45E-31 YLR025W SNF7 hom One of four subunits of the endosomal sorting complex required for transport III (ESCRT-III); involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes
0.197 8.36E-31 YDR456W NHX1 hom Na+/H+ and K+/H+ exchanger, required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion
0.193 2.82E-29 YOR293W RPS10A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication
0.189 1.96E-28 YJR063W RPA12 hom RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex
0.189 2.36E-28 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.183 1.14E-26 YLR417W VPS36 hom Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
0.182 2.19E-26 YIL048W NEO1 het Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
0.179 1.78E-25 YGL072C_d YGL072C_d hom Dubious open reading frame unlikely to encode a protein; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2