YLR165C / PUS5

Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR165C / PUS5

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:3.27|P-value:6.64E-4 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:-3.32|P-value:5.31E-4 ID:SGTC_79|Compound:0052-0007|FD-Score:3.47|P-value:3.34E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:3.70|P-value:1.42E-4 ID:SGTC_111|Compound:0083-0039|FD-Score:-3.18|P-value:8.57E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:-3.17|P-value:8.83E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:4.67|P-value:2.35E-6 ID:SGTC_264|Compound:promethazine|FD-Score:3.24|P-value:7.57E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:3.72|P-value:1.30E-4 ID:SGTC_843|Compound:0310-0051|FD-Score:3.77|P-value:1.09E-4 ID:SGTC_857|Compound:0438-0306|FD-Score:3.27|P-value:6.85E-4 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.55|P-value:2.46E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:-3.29|P-value:5.81E-4 ID:SGTC_1429|Compound:4049-0205|FD-Score:3.28|P-value:6.41E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:3.28|P-value:6.54E-4 ID:SGTC_1683|Compound:st010134|FD-Score:3.21|P-value:8.28E-4 ID:SGTC_1856|Compound:st057054|FD-Score:3.84|P-value:8.30E-5 ID:SGTC_1867|Compound:st057372|FD-Score:3.24|P-value:7.37E-4 ID:SGTC_1993|Compound:st073995|FD-Score:3.24|P-value:7.57E-4 ID:SGTC_1995|Compound:st072814|FD-Score:3.98|P-value:4.69E-5 ID:SGTC_2246|Compound:7293507|FD-Score:3.63|P-value:1.87E-4 ID:SGTC_2260|Compound:7964312|FD-Score:4.19|P-value:1.96E-5 ID:SGTC_2276|Compound:7947513|FD-Score:-3.39|P-value:4.06E-4 ID:SGTC_2327|Compound:6189656|FD-Score:3.39|P-value:4.38E-4 ID:SGTC_2433|Compound:5607874|FD-Score:3.19|P-value:8.77E-4 ID:SGTC_2457|Compound:5731572|FD-Score:-3.40|P-value:3.91E-4 ID:SGTC_2525|Compound:harmine|FD-Score:-3.70|P-value:1.28E-4 ID:SGTC_2547|Compound:epiafzelechin trimethyl ether|FD-Score:3.19|P-value:8.98E-4 ID:SGTC_2678|Compound:equilin|FD-Score:-3.34|P-value:4.93E-4 ID:SGTC_2796|Compound:7949634|FD-Score:4.62|P-value:2.83E-6 ID:SGTC_2800|Compound:7887935|FD-Score:-4.36|P-value:8.57E-6 ID:SGTC_2841|Compound:9004761|FD-Score:7.68|P-value:2.36E-14 ID:SGTC_2844|Compound:9010103|FD-Score:-3.54|P-value:2.41E-4 ID:SGTC_2846|Compound:9008642|FD-Score:-7.65|P-value:2.39E-14 ID:SGTC_2940|Compound:9060638|FD-Score:-3.96|P-value:4.73E-5 ID:SGTC_2998|Compound:9066762|FD-Score:-4.12|P-value:2.37E-5 ID:SGTC_3204|Compound:9114183|FD-Score:3.58|P-value:2.27E-4 ID:SGTC_3236|Compound:9132701|FD-Score:3.42|P-value:3.98E-4 ID:SGTC_3270|Compound:9137563|FD-Score:3.37|P-value:4.76E-4 ID:SGTC_3280|Compound:9140961|FD-Score:3.86|P-value:7.56E-5 ID:SGTC_16|Compound:erodoxin|FD-Score:3.27|P-value:6.64E-4 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:-3.32|P-value:5.31E-4 ID:SGTC_79|Compound:0052-0007|FD-Score:3.47|P-value:3.34E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:3.70|P-value:1.42E-4 ID:SGTC_111|Compound:0083-0039|FD-Score:-3.18|P-value:8.57E-4 ID:SGTC_123|Compound:0083-0120|FD-Score:-3.17|P-value:8.83E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:4.67|P-value:2.35E-6 ID:SGTC_264|Compound:promethazine|FD-Score:3.24|P-value:7.57E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:3.72|P-value:1.30E-4 ID:SGTC_843|Compound:0310-0051|FD-Score:3.77|P-value:1.09E-4 ID:SGTC_857|Compound:0438-0306|FD-Score:3.27|P-value:6.85E-4 ID:SGTC_1076|Compound:itraconazole|FD-Score:3.55|P-value:2.46E-4 ID:SGTC_1318|Compound:1280-3875|FD-Score:-3.29|P-value:5.81E-4 ID:SGTC_1429|Compound:4049-0205|FD-Score:3.28|P-value:6.41E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:3.28|P-value:6.54E-4 ID:SGTC_1683|Compound:st010134|FD-Score:3.21|P-value:8.28E-4 ID:SGTC_1856|Compound:st057054|FD-Score:3.84|P-value:8.30E-5 ID:SGTC_1867|Compound:st057372|FD-Score:3.24|P-value:7.37E-4 ID:SGTC_1993|Compound:st073995|FD-Score:3.24|P-value:7.57E-4 ID:SGTC_1995|Compound:st072814|FD-Score:3.98|P-value:4.69E-5 ID:SGTC_2246|Compound:7293507|FD-Score:3.63|P-value:1.87E-4 ID:SGTC_2260|Compound:7964312|FD-Score:4.19|P-value:1.96E-5 ID:SGTC_2276|Compound:7947513|FD-Score:-3.39|P-value:4.06E-4 ID:SGTC_2327|Compound:6189656|FD-Score:3.39|P-value:4.38E-4 ID:SGTC_2433|Compound:5607874|FD-Score:3.19|P-value:8.77E-4 ID:SGTC_2457|Compound:5731572|FD-Score:-3.40|P-value:3.91E-4 ID:SGTC_2525|Compound:harmine|FD-Score:-3.70|P-value:1.28E-4 ID:SGTC_2547|Compound:epiafzelechin trimethyl ether|FD-Score:3.19|P-value:8.98E-4 ID:SGTC_2678|Compound:equilin|FD-Score:-3.34|P-value:4.93E-4 ID:SGTC_2796|Compound:7949634|FD-Score:4.62|P-value:2.83E-6 ID:SGTC_2800|Compound:7887935|FD-Score:-4.36|P-value:8.57E-6 ID:SGTC_2841|Compound:9004761|FD-Score:7.68|P-value:2.36E-14 ID:SGTC_2844|Compound:9010103|FD-Score:-3.54|P-value:2.41E-4 ID:SGTC_2846|Compound:9008642|FD-Score:-7.65|P-value:2.39E-14 ID:SGTC_2940|Compound:9060638|FD-Score:-3.96|P-value:4.73E-5 ID:SGTC_2998|Compound:9066762|FD-Score:-4.12|P-value:2.37E-5 ID:SGTC_3204|Compound:9114183|FD-Score:3.58|P-value:2.27E-4 ID:SGTC_3236|Compound:9132701|FD-Score:3.42|P-value:3.98E-4 ID:SGTC_3270|Compound:9137563|FD-Score:3.37|P-value:4.76E-4 ID:SGTC_3280|Compound:9140961|FD-Score:3.86|P-value:7.56E-5

Top fitness defect scores for YLR165C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2841 9004761 7.68 39.0 µM 2.36E-14 amide catabolism 4 8.24E-15
2 SGTC_219 mitoxanthrone 4.67 164.4 µM 2.35E-6 DNA intercalators 35 1.54E-6
3 SGTC_2796 7949634 4.62 45.5 µM 2.83E-6 11 1.87E-6
4 SGTC_2260 7964312 4.19 200.0 µM 1.96E-5 10 1.38E-5
5 SGTC_1995 st072814 3.98 61.1 µM 4.69E-5 13 3.41E-5
6 SGTC_3280 9140961 3.86 49.5 µM 7.56E-5 60S ribosome export 17 5.60E-5
7 SGTC_1856 st057054 3.84 55.7 µM 8.30E-5 30 6.17E-5
8 SGTC_843 0310-0051 3.77 143.0 µM 1.09E-4 14 8.17E-5
9 SGTC_796 1234-0020 3.72 70.7 µM 1.30E-4 SWF1 & branched chain AA biosynthesis 30 9.83E-5
10 SGTC_98 0074-0023 3.70 107.7 µM 1.42E-4 Golgi 46 1.07E-4
11 SGTC_2246 7293507 3.63 91.6 µM 1.87E-4 37 1.43E-4
12 SGTC_3204 9114183 3.58 49.5 µM 2.27E-4 45 1.74E-4
13 SGTC_1076 itraconazole 3.55 331.0 nM 2.46E-4 47 1.90E-4
14 SGTC_79 0052-0007 3.47 94.1 µM 3.34E-4 55 2.60E-4
15 SGTC_3236 9132701 3.42 49.5 µM 3.98E-4 TSC3-RPN4 42 3.12E-4
16 SGTC_2327 6189656 3.39 200.0 µM 4.38E-4 37 3.44E-4
17 SGTC_3270 9137563 3.37 49.5 µM 4.76E-4 RPP1 & pyrimidine depletion 15 3.75E-4
18 SGTC_1429 4049-0205 3.28 9.5 µM 6.41E-4 29 5.10E-4
19 SGTC_1475 4092-1048 3.28 11.7 µM 6.54E-4 plasma membrane duress 64 5.21E-4
20 SGTC_16 erodoxin 3.27 22.8 µM 6.64E-4 unfolded protein response 96 5.29E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.131 2.46E-14 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.131 2.95E-14 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.125 3.72E-13 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.097 1.86E-8 YKR087C OMA1 hom Metalloendopeptidase of the mitochondrial inner membrane; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; member of a family of predicted membrane-bound metallopeptidases in prokaryotes and higher eukaryotes
0.095 3.99E-8 YOR064C YNG1 hom Subunit of the NuA3 histone acetyltransferase complex that acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3, has similarity to the human tumor suppressor ING1
0.094 5.77E-8 YGR070W ROM1 hom GDP/GTP exchange protein (GEP) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP
0.088 3.42E-7 YPL219W PCL8 hom Cyclin, interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation
0.087 3.93E-7 YKR056W TRM2 hom tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair
0.087 4.84E-7 YIR043C YIR043C hom Possible pseudogene in strain S288C; YIR043C and the adjacent ORF, YIR044C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family
0.086 6.21E-7 YJL126W NIT2 hom Nit protein, one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member
0.082 1.79E-6 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.082 2.08E-6 YDR113C PDS1 het Securin, inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation
0.082 2.23E-6 YPL244C HUT1 hom Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1
0.080 3.17E-6 YDL045C FAD1 het Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
0.079 5.06E-6 YNL175C NOP13 hom Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress