YLR172C / DPH5

Methyltransferase required for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR172C / DPH5

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:4.61|P-value:2.74E-6 ID:SGTC_45|Compound:0kpi-0036|FD-Score:5.81|P-value:4.91E-9 ID:SGTC_58|Compound:0957-0393|FD-Score:4.32|P-value:1.05E-5 ID:SGTC_65|Compound:0798-0905|FD-Score:3.32|P-value:5.60E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:3.58|P-value:2.20E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.96|P-value:4.86E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:5.60|P-value:1.65E-8 ID:SGTC_274|Compound:sirtinol|FD-Score:3.19|P-value:8.61E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:3.53|P-value:2.65E-4 ID:SGTC_385|Compound:1494-0393|FD-Score:3.52|P-value:2.68E-4 ID:SGTC_397|Compound:arbidol|FD-Score:3.39|P-value:4.34E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:4.31|P-value:1.11E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:3.39|P-value:4.41E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:3.85|P-value:7.72E-5 ID:SGTC_550|Compound:4123-0385|FD-Score:3.53|P-value:2.61E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:4.52|P-value:4.19E-6 ID:SGTC_611|Compound:3453-1652|FD-Score:3.64|P-value:1.74E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:4.66|P-value:2.18E-6 ID:SGTC_757|Compound:1066-0008|FD-Score:4.88|P-value:7.60E-7 ID:SGTC_815|Compound:1582-0027|FD-Score:3.76|P-value:1.08E-4 ID:SGTC_823|Compound:0417-1665|FD-Score:5.00|P-value:4.21E-7 ID:SGTC_881|Compound:0833-0569|FD-Score:4.05|P-value:3.38E-5 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.34|P-value:3.90E-4 ID:SGTC_895|Compound:0964-0006|FD-Score:4.92|P-value:6.17E-7 ID:SGTC_964|Compound:1287-0107|FD-Score:4.16|P-value:2.10E-5 ID:SGTC_972|Compound:1319-0219|FD-Score:3.60|P-value:2.03E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:4.14|P-value:2.30E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.43|P-value:3.70E-4 ID:SGTC_1064|Compound:honokiol|FD-Score:4.56|P-value:3.58E-6 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:-3.32|P-value:4.18E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.26|P-value:6.85E-4 ID:SGTC_1228|Compound:0124-0032|FD-Score:4.02|P-value:3.75E-5 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.64|P-value:1.71E-4 ID:SGTC_1364|Compound:1653-0028|FD-Score:4.13|P-value:2.36E-5 ID:SGTC_1377|Compound:2944-0135|FD-Score:3.82|P-value:8.44E-5 ID:SGTC_1396|Compound:3511-0007|FD-Score:3.42|P-value:3.90E-4 ID:SGTC_1426|Compound:4048-4480|FD-Score:3.25|P-value:7.13E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.24|P-value:5.61E-4 ID:SGTC_1730|Compound:st033808|FD-Score:3.58|P-value:2.17E-4 ID:SGTC_1754|Compound:st042065|FD-Score:7.73|P-value:1.10E-14 ID:SGTC_1760|Compound:st045453|FD-Score:5.71|P-value:8.94E-9 ID:SGTC_1781|Compound:st038725|FD-Score:5.31|P-value:8.08E-8 ID:SGTC_1800|Compound:st050079|FD-Score:7.15|P-value:7.94E-13 ID:SGTC_1801|Compound:st050089|FD-Score:3.39|P-value:4.38E-4 ID:SGTC_1803|Compound:st050068|FD-Score:6.49|P-value:7.03E-11 ID:SGTC_1818|Compound:st052136|FD-Score:3.31|P-value:5.76E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:3.85|P-value:7.53E-5 ID:SGTC_1941|Compound:st076221|FD-Score:4.88|P-value:7.69E-7 ID:SGTC_2301|Compound:7769476|FD-Score:3.44|P-value:3.68E-4 ID:SGTC_2302|Compound:7771753|FD-Score:-7.70|P-value:7.34E-15 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:5.77|P-value:6.17E-9 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:4.35|P-value:9.34E-6 ID:SGTC_2583|Compound:asiatic acid|FD-Score:-3.19|P-value:6.75E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:4.35|P-value:9.23E-6 ID:SGTC_2601|Compound:securinine|FD-Score:4.71|P-value:1.72E-6 ID:SGTC_2651|Compound:1327-0036|FD-Score:4.42|P-value:6.77E-6 ID:SGTC_2835|Compound:9006588|FD-Score:-3.87|P-value:5.02E-5 ID:SGTC_2851|Compound:9015922|FD-Score:4.98|P-value:4.48E-7 ID:SGTC_2858|Compound:9025781|FD-Score:8.97|P-value:3.53E-19 ID:SGTC_2859|Compound:9028643|FD-Score:-3.18|P-value:6.79E-4 ID:SGTC_2882|Compound:9058044|FD-Score:-6.01|P-value:9.31E-10 ID:SGTC_2884|Compound:9058502|FD-Score:4.76|P-value:1.35E-6 ID:SGTC_2896|Compound:9052656|FD-Score:7.52|P-value:5.37E-14 ID:SGTC_3037|Compound:9091026|FD-Score:6.19|P-value:4.76E-10 ID:SGTC_3038|Compound:9091290|FD-Score:3.48|P-value:3.13E-4 ID:SGTC_3109|Compound:9122853|FD-Score:3.60|P-value:2.02E-4 ID:SGTC_3286|Compound:9117135|FD-Score:-3.53|P-value:1.97E-4 ID:SGTC_3301|Compound:9125354|FD-Score:4.97|P-value:4.70E-7 ID:SGTC_3309|Compound:9129340|FD-Score:7.17|P-value:6.84E-13 ID:SGTC_3310|Compound:9130229|FD-Score:4.65|P-value:2.33E-6 ID:SGTC_3330|Compound:9141424|FD-Score:-3.54|P-value:1.91E-4 ID:SGTC_3334|Compound:9142812|FD-Score:4.76|P-value:1.33E-6 ID:SGTC_3339|Compound:9145883|FD-Score:4.24|P-value:1.50E-5 ID:SGTC_37|Compound:0566-0070|FD-Score:4.61|P-value:2.74E-6 ID:SGTC_45|Compound:0kpi-0036|FD-Score:5.81|P-value:4.91E-9 ID:SGTC_58|Compound:0957-0393|FD-Score:4.32|P-value:1.05E-5 ID:SGTC_65|Compound:0798-0905|FD-Score:3.32|P-value:5.60E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:3.58|P-value:2.20E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:3.96|P-value:4.86E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:5.60|P-value:1.65E-8 ID:SGTC_274|Compound:sirtinol|FD-Score:3.19|P-value:8.61E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:3.53|P-value:2.65E-4 ID:SGTC_385|Compound:1494-0393|FD-Score:3.52|P-value:2.68E-4 ID:SGTC_397|Compound:arbidol|FD-Score:3.39|P-value:4.34E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:4.31|P-value:1.11E-5 ID:SGTC_513|Compound:4245-1575|FD-Score:3.39|P-value:4.41E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:3.85|P-value:7.72E-5 ID:SGTC_550|Compound:4123-0385|FD-Score:3.53|P-value:2.61E-4 ID:SGTC_610|Compound:4215-0184|FD-Score:4.52|P-value:4.19E-6 ID:SGTC_611|Compound:3453-1652|FD-Score:3.64|P-value:1.74E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:4.66|P-value:2.18E-6 ID:SGTC_757|Compound:1066-0008|FD-Score:4.88|P-value:7.60E-7 ID:SGTC_815|Compound:1582-0027|FD-Score:3.76|P-value:1.08E-4 ID:SGTC_823|Compound:0417-1665|FD-Score:5.00|P-value:4.21E-7 ID:SGTC_881|Compound:0833-0569|FD-Score:4.05|P-value:3.38E-5 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.34|P-value:3.90E-4 ID:SGTC_895|Compound:0964-0006|FD-Score:4.92|P-value:6.17E-7 ID:SGTC_964|Compound:1287-0107|FD-Score:4.16|P-value:2.10E-5 ID:SGTC_972|Compound:1319-0219|FD-Score:3.60|P-value:2.03E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:4.14|P-value:2.30E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.43|P-value:3.70E-4 ID:SGTC_1064|Compound:honokiol|FD-Score:4.56|P-value:3.58E-6 ID:SGTC_1069|Compound:diphenylcyclopropenone|FD-Score:-3.32|P-value:4.18E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:3.26|P-value:6.85E-4 ID:SGTC_1228|Compound:0124-0032|FD-Score:4.02|P-value:3.75E-5 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.64|P-value:1.71E-4 ID:SGTC_1364|Compound:1653-0028|FD-Score:4.13|P-value:2.36E-5 ID:SGTC_1377|Compound:2944-0135|FD-Score:3.82|P-value:8.44E-5 ID:SGTC_1396|Compound:3511-0007|FD-Score:3.42|P-value:3.90E-4 ID:SGTC_1426|Compound:4048-4480|FD-Score:3.25|P-value:7.13E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-3.24|P-value:5.61E-4 ID:SGTC_1730|Compound:st033808|FD-Score:3.58|P-value:2.17E-4 ID:SGTC_1754|Compound:st042065|FD-Score:7.73|P-value:1.10E-14 ID:SGTC_1760|Compound:st045453|FD-Score:5.71|P-value:8.94E-9 ID:SGTC_1781|Compound:st038725|FD-Score:5.31|P-value:8.08E-8 ID:SGTC_1800|Compound:st050079|FD-Score:7.15|P-value:7.94E-13 ID:SGTC_1801|Compound:st050089|FD-Score:3.39|P-value:4.38E-4 ID:SGTC_1803|Compound:st050068|FD-Score:6.49|P-value:7.03E-11 ID:SGTC_1818|Compound:st052136|FD-Score:3.31|P-value:5.76E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:3.85|P-value:7.53E-5 ID:SGTC_1941|Compound:st076221|FD-Score:4.88|P-value:7.69E-7 ID:SGTC_2301|Compound:7769476|FD-Score:3.44|P-value:3.68E-4 ID:SGTC_2302|Compound:7771753|FD-Score:-7.70|P-value:7.34E-15 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:5.77|P-value:6.17E-9 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:4.35|P-value:9.34E-6 ID:SGTC_2583|Compound:asiatic acid|FD-Score:-3.19|P-value:6.75E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:4.35|P-value:9.23E-6 ID:SGTC_2601|Compound:securinine|FD-Score:4.71|P-value:1.72E-6 ID:SGTC_2651|Compound:1327-0036|FD-Score:4.42|P-value:6.77E-6 ID:SGTC_2835|Compound:9006588|FD-Score:-3.87|P-value:5.02E-5 ID:SGTC_2851|Compound:9015922|FD-Score:4.98|P-value:4.48E-7 ID:SGTC_2858|Compound:9025781|FD-Score:8.97|P-value:3.53E-19 ID:SGTC_2859|Compound:9028643|FD-Score:-3.18|P-value:6.79E-4 ID:SGTC_2882|Compound:9058044|FD-Score:-6.01|P-value:9.31E-10 ID:SGTC_2884|Compound:9058502|FD-Score:4.76|P-value:1.35E-6 ID:SGTC_2896|Compound:9052656|FD-Score:7.52|P-value:5.37E-14 ID:SGTC_3037|Compound:9091026|FD-Score:6.19|P-value:4.76E-10 ID:SGTC_3038|Compound:9091290|FD-Score:3.48|P-value:3.13E-4 ID:SGTC_3109|Compound:9122853|FD-Score:3.60|P-value:2.02E-4 ID:SGTC_3286|Compound:9117135|FD-Score:-3.53|P-value:1.97E-4 ID:SGTC_3301|Compound:9125354|FD-Score:4.97|P-value:4.70E-7 ID:SGTC_3309|Compound:9129340|FD-Score:7.17|P-value:6.84E-13 ID:SGTC_3310|Compound:9130229|FD-Score:4.65|P-value:2.33E-6 ID:SGTC_3330|Compound:9141424|FD-Score:-3.54|P-value:1.91E-4 ID:SGTC_3334|Compound:9142812|FD-Score:4.76|P-value:1.33E-6 ID:SGTC_3339|Compound:9145883|FD-Score:4.24|P-value:1.50E-5

Top fitness defect scores for YLR172C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2858 9025781 8.97 9.0 µM 3.53E-19 sphingolipid biosynthesis & PDR1 24 1.51E-19
2 SGTC_1754 st042065 7.73 16.1 µM 1.10E-14 NEO1-PIK1 61 5.56E-15
3 SGTC_2896 9052656 7.52 71.4 µM 5.37E-14 endomembrane recycling 23 2.81E-14
4 SGTC_3309 9129340 7.17 25.8 µM 6.84E-13 2 3.74E-13
5 SGTC_1800 st050079 7.15 29.1 µM 7.94E-13 NEO1-PIK1 22 4.35E-13
6 SGTC_1803 st050068 6.49 52.4 µM 7.03E-11 NEO1-PIK1 67 4.17E-11
7 SGTC_3037 9091026 6.19 49.5 µM 4.76E-10 NEO1-PIK1 44 2.93E-10
8 SGTC_45 0kpi-0036 5.81 14.9 µM 4.91E-9 excess fatty acid 5 3.15E-9
9 SGTC_2536 dehydroabietamide 5.77 32.0 µM 6.17E-9 5 3.97E-9
10 SGTC_1760 st045453 5.71 10.4 µM 8.94E-9 NEO1-PIK1 89 5.80E-9
11 SGTC_255 tunicamycin 5.60 200.5 nM 1.65E-8 unfolded protein response 37 1.08E-8
12 SGTC_1781 st038725 5.31 66.3 µM 8.08E-8 NEO1 67 5.46E-8
13 SGTC_823 0417-1665 5.00 178.0 µM 4.21E-7 24 2.94E-7
14 SGTC_2851 9015922 4.98 71.4 µM 4.48E-7 16 3.13E-7
15 SGTC_3301 9125354 4.97 19.8 µM 4.70E-7 5 3.29E-7
16 SGTC_895 0964-0006 4.92 13.7 µM 6.17E-7 2 4.34E-7
17 SGTC_757 1066-0008 4.88 322.0 µM 7.60E-7 Golgi 8 5.37E-7
18 SGTC_1941 st076221 4.88 61.8 µM 7.69E-7 sphingolipid biosynthesis & PDR1 50 5.43E-7
19 SGTC_3334 9142812 4.76 21.9 µM 1.33E-6 14 9.50E-7
20 SGTC_2884 9058502 4.76 52.0 µM 1.35E-6 15 9.64E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.304 6.82E-73 YLR020C YEH2 hom Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism
0.288 3.94E-65 YBR246W RRT2 hom Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.263 3.23E-54 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.234 4.61E-43 YDR084C TVP23 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.233 1.04E-42 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.231 6.83E-42 YOR115C TRS33 hom One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.228 6.45E-41 YKL191W DPH2 hom Protein required, along with Dph1p, Kti11p, Jjj3p, and Dph5p, for synthesis of diphthamide, which is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); may act in a complex with Dph1p and Kti11p
0.228 1.25E-40 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.225 7.06E-40 YML097C VPS9 hom A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
0.214 3.88E-36 YDR492W IZH1 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc
0.211 3.25E-35 YJR097W JJJ3 hom Protein of unknown function, contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4
0.211 3.34E-35 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.210 9.64E-35 YBR245C ISW1 hom ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; ATPase; forms a complex with Ioc3p (Isw1a), and a complex with Ioc2p and Ioc4p (Isw1b); Isw1a and Isw1b have partially overlapping and distinct roles, Isw1a involved in repression of transcription initiation and Isw1b involved in regulation of transcription elongation; Isw1b recruited to open reading frames by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions
0.208 3.82E-34 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.206 1.48E-33 YPR092W_d YPR092W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data