YLR174W / IDP2

Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR174W / IDP2

Click on Significant Values for Screen Details ID:SGTC_50|Compound:0929-0078|FD-Score:-3.26|P-value:1.64E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:3.12|P-value:2.68E-4 ID:SGTC_264|Compound:promethazine|FD-Score:3.22|P-value:1.77E-4 ID:SGTC_269|Compound:exalamide|FD-Score:3.03|P-value:3.97E-4 ID:SGTC_276|Compound:colchicine|FD-Score:-3.55|P-value:4.55E-5 ID:SGTC_379|Compound:3970-0972|FD-Score:-4.21|P-value:1.75E-6 ID:SGTC_389|Compound:tpck|FD-Score:-3.31|P-value:1.33E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.19|P-value:2.03E-4 ID:SGTC_469|Compound:w7|FD-Score:3.42|P-value:7.46E-5 ID:SGTC_624|Compound:k048-0052|FD-Score:3.04|P-value:3.67E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:-5.54|P-value:4.81E-10 ID:SGTC_788|Compound:1182-0090|FD-Score:2.96|P-value:5.04E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:5.23|P-value:3.54E-9 ID:SGTC_827|Compound:3253-0474|FD-Score:3.10|P-value:2.95E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:4.27|P-value:1.12E-6 ID:SGTC_931|Compound:2812-0076|FD-Score:-3.73|P-value:2.00E-5 ID:SGTC_1068|Compound:loratadine|FD-Score:-4.01|P-value:4.97E-6 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:3.34|P-value:1.07E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:-7.73|P-value:7.04E-18 ID:SGTC_1246|Compound:0422-0085|FD-Score:-3.05|P-value:3.80E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:7.72|P-value:6.38E-18 ID:SGTC_1248|Compound:0469-0687|FD-Score:6.90|P-value:1.11E-14 ID:SGTC_1277|Compound:0873-0036|FD-Score:3.46|P-value:6.16E-5 ID:SGTC_1280|Compound:0880-0253|FD-Score:4.10|P-value:2.78E-6 ID:SGTC_1373|Compound:2887-0003|FD-Score:3.38|P-value:8.78E-5 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.16|P-value:2.29E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.13|P-value:2.54E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:-3.39|P-value:9.26E-5 ID:SGTC_1745|Compound:st081495|FD-Score:-3.04|P-value:3.98E-4 ID:SGTC_1940|Compound:st075990|FD-Score:2.96|P-value:5.04E-4 ID:SGTC_2155|Compound:5570423|FD-Score:2.86|P-value:7.63E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:-3.13|P-value:2.81E-4 ID:SGTC_2428|Compound:5615643|FD-Score:4.75|P-value:7.20E-8 ID:SGTC_2436|Compound:5633522|FD-Score:5.01|P-value:1.40E-8 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:-2.84|P-value:8.67E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:3.00|P-value:4.46E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:2.83|P-value:8.42E-4 ID:SGTC_2724|Compound:maprotiline|FD-Score:3.10|P-value:2.91E-4 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:3.31|P-value:1.21E-4 ID:SGTC_2822|Compound:7994718|FD-Score:-4.64|P-value:1.51E-7 ID:SGTC_2823|Compound:7987624|FD-Score:-3.19|P-value:2.20E-4 ID:SGTC_2836|Compound:9010338|FD-Score:-4.97|P-value:2.13E-8 ID:SGTC_2838|Compound:9002687|FD-Score:-3.89|P-value:9.00E-6 ID:SGTC_2841|Compound:9004761|FD-Score:-3.76|P-value:1.69E-5 ID:SGTC_2846|Compound:9008642|FD-Score:3.38|P-value:8.80E-5 ID:SGTC_3033|Compound:9090887|FD-Score:3.35|P-value:1.04E-4 ID:SGTC_3310|Compound:9130229|FD-Score:3.95|P-value:5.87E-6 ID:SGTC_50|Compound:0929-0078|FD-Score:-3.26|P-value:1.64E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:3.12|P-value:2.68E-4 ID:SGTC_264|Compound:promethazine|FD-Score:3.22|P-value:1.77E-4 ID:SGTC_269|Compound:exalamide|FD-Score:3.03|P-value:3.97E-4 ID:SGTC_276|Compound:colchicine|FD-Score:-3.55|P-value:4.55E-5 ID:SGTC_379|Compound:3970-0972|FD-Score:-4.21|P-value:1.75E-6 ID:SGTC_389|Compound:tpck|FD-Score:-3.31|P-value:1.33E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:3.19|P-value:2.03E-4 ID:SGTC_469|Compound:w7|FD-Score:3.42|P-value:7.46E-5 ID:SGTC_624|Compound:k048-0052|FD-Score:3.04|P-value:3.67E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:-5.54|P-value:4.81E-10 ID:SGTC_788|Compound:1182-0090|FD-Score:2.96|P-value:5.04E-4 ID:SGTC_824|Compound:1304-0008|FD-Score:5.23|P-value:3.54E-9 ID:SGTC_827|Compound:3253-0474|FD-Score:3.10|P-value:2.95E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:4.27|P-value:1.12E-6 ID:SGTC_931|Compound:2812-0076|FD-Score:-3.73|P-value:2.00E-5 ID:SGTC_1068|Compound:loratadine|FD-Score:-4.01|P-value:4.97E-6 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:3.34|P-value:1.07E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:-7.73|P-value:7.04E-18 ID:SGTC_1246|Compound:0422-0085|FD-Score:-3.05|P-value:3.80E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:7.72|P-value:6.38E-18 ID:SGTC_1248|Compound:0469-0687|FD-Score:6.90|P-value:1.11E-14 ID:SGTC_1277|Compound:0873-0036|FD-Score:3.46|P-value:6.16E-5 ID:SGTC_1280|Compound:0880-0253|FD-Score:4.10|P-value:2.78E-6 ID:SGTC_1373|Compound:2887-0003|FD-Score:3.38|P-value:8.78E-5 ID:SGTC_1378|Compound:2093-0007|FD-Score:3.16|P-value:2.29E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:3.13|P-value:2.54E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:-3.39|P-value:9.26E-5 ID:SGTC_1745|Compound:st081495|FD-Score:-3.04|P-value:3.98E-4 ID:SGTC_1940|Compound:st075990|FD-Score:2.96|P-value:5.04E-4 ID:SGTC_2155|Compound:5570423|FD-Score:2.86|P-value:7.63E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:-3.13|P-value:2.81E-4 ID:SGTC_2428|Compound:5615643|FD-Score:4.75|P-value:7.20E-8 ID:SGTC_2436|Compound:5633522|FD-Score:5.01|P-value:1.40E-8 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:-2.84|P-value:8.67E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:3.00|P-value:4.46E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:2.83|P-value:8.42E-4 ID:SGTC_2724|Compound:maprotiline|FD-Score:3.10|P-value:2.91E-4 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:3.31|P-value:1.21E-4 ID:SGTC_2822|Compound:7994718|FD-Score:-4.64|P-value:1.51E-7 ID:SGTC_2823|Compound:7987624|FD-Score:-3.19|P-value:2.20E-4 ID:SGTC_2836|Compound:9010338|FD-Score:-4.97|P-value:2.13E-8 ID:SGTC_2838|Compound:9002687|FD-Score:-3.89|P-value:9.00E-6 ID:SGTC_2841|Compound:9004761|FD-Score:-3.76|P-value:1.69E-5 ID:SGTC_2846|Compound:9008642|FD-Score:3.38|P-value:8.80E-5 ID:SGTC_3033|Compound:9090887|FD-Score:3.35|P-value:1.04E-4 ID:SGTC_3310|Compound:9130229|FD-Score:3.95|P-value:5.87E-6

Top fitness defect scores for YLR174W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1247 0448-0043 7.72 5.8 µM 6.38E-18 ERG2 3 5.82E-15
2 SGTC_1248 0469-0687 6.90 58.6 µM 1.11E-14 4 2.63E-12
3 SGTC_824 1304-0008 5.23 100.0 µM 3.54E-9 DNA damage response 18 8.65E-8
4 SGTC_2436 5633522 5.01 8.1 µM 1.40E-8 7 2.68E-7
5 SGTC_2428 5615643 4.75 7.8 µM 7.20E-8 24 1.03E-6
6 SGTC_833 3852-0053 4.27 558.0 µM 1.12E-6 12 9.79E-6
7 SGTC_1280 0880-0253 4.10 10.1 µM 2.78E-6 14 2.08E-5
8 SGTC_3310 9130229 3.95 44.1 µM 5.87E-6 Golgi 27 3.84E-5
9 SGTC_1277 0873-0036 3.46 22.0 µM 6.16E-5 PDR1 41 2.67E-4
10 SGTC_469 w7 3.42 172.0 µM 7.46E-5 ERG2 20 3.12E-4
11 SGTC_1373 2887-0003 3.38 58.5 µM 8.78E-5 Golgi 39 3.58E-4
12 SGTC_2846 9008642 3.38 19.5 µM 8.80E-5 31 3.58E-4
13 SGTC_3033 9090887 3.35 49.5 µM 1.04E-4 fatty acid desaturase (OLE1) 50 4.10E-4
14 SGTC_1071 trifluoperazine 3.34 6.3 µM 1.07E-4 44 4.22E-4
15 SGTC_2758 dehydroepiandrosterone 3.31 69.3 µM 1.21E-4 69 4.66E-4
16 SGTC_264 promethazine 3.22 12.5 mM 1.77E-4 DNA intercalators 103 6.37E-4
17 SGTC_464 bw-b 70c 3.19 158.0 µM 2.03E-4 60 7.15E-4
18 SGTC_1378 2093-0007 3.16 380.0 nM 2.29E-4 52 7.88E-4
19 SGTC_1525 8-methoxypsoralen 3.13 92.5 µM 2.54E-4 DNA damage response 73 8.61E-4
20 SGTC_65 0798-0905 3.12 35.0 µM 2.68E-4 calcium & mitochondrial duress 152 8.99E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.099 9.81E-9 YDR210W_p YDR210W_p hom Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.097 1.69E-8 YPR159C-A_p YPR159C-A_p hom Identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.097 1.80E-8 YCR050C_p YCR050C_p hom Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein
0.096 2.92E-8 YDR517W GRH1 hom Acetylated cis-Golgi protein, involved in ER to Golgi transport; homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes; protein abundance increases in response to DNA replication stress
0.092 8.60E-8 YLR082C SRL2 hom Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation
0.092 1.02E-7 YER085C_p YER085C_p hom Putative protein of unknown function
0.090 1.65E-7 YKL131W_d YKL131W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.089 2.83E-7 YGL010W_p YGL010W_p hom Putative protein of unknown function; YGL010W is not an essential gene
0.088 2.97E-7 YLR060W FRS1 het Beta subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p to generate active enzyme; able to hydrolyze mis-aminoacylated tRNA-Phe, which could contribute to translational quality control
0.088 3.01E-7 YEL020C_p YEL020C_p hom Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data
0.088 3.62E-7 YDR193W_d YDR193W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.085 8.82E-7 YOR181W LAS17 het Actin assembly factor, activates the Arp2/3 protein complex that nucleates branched actin filaments; localizes with the Arp2/3 complex to actin patches; homolog of the human Wiskott-Aldrich syndrome protein (WASP)
0.084 1.04E-6 YOR133W EFT1 hom Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.081 2.53E-6 YDR471W RPL27B hom Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication
0.079 4.77E-6 YPR079W MRL1 hom Membrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress