YLR175W / CBF5

Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR175W / CBF5

Click on Significant Values for Screen Details ID:SGTC_210|Compound:3551-0445|FD-Score:3.18|P-value:3.45E-4 ID:SGTC_441|Compound:propafenone|FD-Score:2.90|P-value:9.78E-4 ID:SGTC_494|Compound:Boc-Gly-Val-Val-CHO|FD-Score:4.98|P-value:5.35E-8 ID:SGTC_496|Compound:calpeptin|FD-Score:-3.53|P-value:8.51E-5 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:2.91|P-value:9.32E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:3.05|P-value:5.71E-4 ID:SGTC_650|Compound:1328-0195|FD-Score:3.86|P-value:1.89E-5 ID:SGTC_705|Compound:1124-0212|FD-Score:3.28|P-value:2.35E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:5.38|P-value:4.90E-9 ID:SGTC_815|Compound:1582-0027|FD-Score:3.12|P-value:4.40E-4 ID:SGTC_983|Compound:1491-0821|FD-Score:3.51|P-value:8.85E-5 ID:SGTC_1183|Compound:1254-0324|FD-Score:-3.19|P-value:3.48E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.11|P-value:4.58E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:-3.06|P-value:5.65E-4 ID:SGTC_1389|Compound:3405-0187|FD-Score:4.50|P-value:8.11E-7 ID:SGTC_1390|Compound:3413-2614|FD-Score:2.99|P-value:7.12E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:4.32|P-value:2.07E-6 ID:SGTC_1511|Compound:0906-3109|FD-Score:-3.62|P-value:5.92E-5 ID:SGTC_1685|Compound:st024049|FD-Score:3.48|P-value:1.04E-4 ID:SGTC_1733|Compound:st037357|FD-Score:4.06|P-value:7.46E-6 ID:SGTC_1736|Compound:st036774|FD-Score:-3.01|P-value:6.77E-4 ID:SGTC_1910|Compound:st062352|FD-Score:-3.29|P-value:2.32E-4 ID:SGTC_1919|Compound:st067113|FD-Score:-5.65|P-value:9.07E-10 ID:SGTC_1991|Compound:st073000|FD-Score:3.29|P-value:2.20E-4 ID:SGTC_2033|Compound:5192550|FD-Score:3.64|P-value:5.20E-5 ID:SGTC_2165|Compound:5727197|FD-Score:3.11|P-value:4.53E-4 ID:SGTC_2179|Compound:6298986|FD-Score:2.97|P-value:7.76E-4 ID:SGTC_2285|Compound:7945142|FD-Score:3.24|P-value:2.73E-4 ID:SGTC_2316|Compound:5844804|FD-Score:-3.14|P-value:4.22E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:3.82|P-value:2.34E-5 ID:SGTC_2649|Compound:anisomycin|FD-Score:3.47|P-value:1.05E-4 ID:SGTC_2929|Compound:9013348|FD-Score:3.12|P-value:4.40E-4 ID:SGTC_2951|Compound:9072950|FD-Score:2.91|P-value:9.63E-4 ID:SGTC_2975|Compound:9084847|FD-Score:-3.61|P-value:6.01E-5 ID:SGTC_2977|Compound:9011197|FD-Score:-3.10|P-value:4.78E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-4.98|P-value:5.70E-8 ID:SGTC_3299|Compound:9121982|FD-Score:-3.36|P-value:1.73E-4 ID:SGTC_3310|Compound:9130229|FD-Score:-2.92|P-value:9.54E-4 ID:SGTC_3326|Compound:9140064|FD-Score:3.09|P-value:4.85E-4 ID:SGTC_210|Compound:3551-0445|FD-Score:3.18|P-value:3.45E-4 ID:SGTC_441|Compound:propafenone|FD-Score:2.90|P-value:9.78E-4 ID:SGTC_494|Compound:Boc-Gly-Val-Val-CHO|FD-Score:4.98|P-value:5.35E-8 ID:SGTC_496|Compound:calpeptin|FD-Score:-3.53|P-value:8.51E-5 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:2.91|P-value:9.32E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:3.05|P-value:5.71E-4 ID:SGTC_650|Compound:1328-0195|FD-Score:3.86|P-value:1.89E-5 ID:SGTC_705|Compound:1124-0212|FD-Score:3.28|P-value:2.35E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:5.38|P-value:4.90E-9 ID:SGTC_815|Compound:1582-0027|FD-Score:3.12|P-value:4.40E-4 ID:SGTC_983|Compound:1491-0821|FD-Score:3.51|P-value:8.85E-5 ID:SGTC_1183|Compound:1254-0324|FD-Score:-3.19|P-value:3.48E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.11|P-value:4.58E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:-3.06|P-value:5.65E-4 ID:SGTC_1389|Compound:3405-0187|FD-Score:4.50|P-value:8.11E-7 ID:SGTC_1390|Compound:3413-2614|FD-Score:2.99|P-value:7.12E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:4.32|P-value:2.07E-6 ID:SGTC_1511|Compound:0906-3109|FD-Score:-3.62|P-value:5.92E-5 ID:SGTC_1685|Compound:st024049|FD-Score:3.48|P-value:1.04E-4 ID:SGTC_1733|Compound:st037357|FD-Score:4.06|P-value:7.46E-6 ID:SGTC_1736|Compound:st036774|FD-Score:-3.01|P-value:6.77E-4 ID:SGTC_1910|Compound:st062352|FD-Score:-3.29|P-value:2.32E-4 ID:SGTC_1919|Compound:st067113|FD-Score:-5.65|P-value:9.07E-10 ID:SGTC_1991|Compound:st073000|FD-Score:3.29|P-value:2.20E-4 ID:SGTC_2033|Compound:5192550|FD-Score:3.64|P-value:5.20E-5 ID:SGTC_2165|Compound:5727197|FD-Score:3.11|P-value:4.53E-4 ID:SGTC_2179|Compound:6298986|FD-Score:2.97|P-value:7.76E-4 ID:SGTC_2285|Compound:7945142|FD-Score:3.24|P-value:2.73E-4 ID:SGTC_2316|Compound:5844804|FD-Score:-3.14|P-value:4.22E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:3.82|P-value:2.34E-5 ID:SGTC_2649|Compound:anisomycin|FD-Score:3.47|P-value:1.05E-4 ID:SGTC_2929|Compound:9013348|FD-Score:3.12|P-value:4.40E-4 ID:SGTC_2951|Compound:9072950|FD-Score:2.91|P-value:9.63E-4 ID:SGTC_2975|Compound:9084847|FD-Score:-3.61|P-value:6.01E-5 ID:SGTC_2977|Compound:9011197|FD-Score:-3.10|P-value:4.78E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-4.98|P-value:5.70E-8 ID:SGTC_3299|Compound:9121982|FD-Score:-3.36|P-value:1.73E-4 ID:SGTC_3310|Compound:9130229|FD-Score:-2.92|P-value:9.54E-4 ID:SGTC_3326|Compound:9140064|FD-Score:3.09|P-value:4.85E-4

Top fitness defect scores for YLR175W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_738 1315-0003 5.38 72.4 µM 4.90E-9 2 3.80E-8 0.55
2 SGTC_494 Boc-Gly-Val-Val-CHO 4.98 140.0 µM 5.35E-8 1 3.14E-7 1.12
3 SGTC_1389 3405-0187 4.50 19.0 µM 8.11E-7 1 3.47E-6 0.49
4 SGTC_1393 3470-5649 4.32 58.2 µM 2.07E-6 60S ribosome export 2 7.96E-6 0.15
5 SGTC_1733 st037357 4.06 54.9 µM 7.46E-6 60S ribosome export 5 2.47E-5 0.01
6 SGTC_650 1328-0195 3.86 38.0 µM 1.89E-5 2 5.63E-5 0.02
7 SGTC_2530 3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene 3.82 92.6 µM 2.34E-5 12 6.80E-5 0.32
8 SGTC_2033 5192550 3.64 145.0 µM 5.20E-5 5 1.38E-4 0.05
9 SGTC_983 1491-0821 3.51 12.2 µM 8.85E-5 9 2.20E-4 0.03
10 SGTC_1685 st024049 3.48 53.1 µM 1.04E-4 TSC3-RPN4 3 2.54E-4 0.32
11 SGTC_2649 anisomycin 3.47 9.6 µM 1.05E-4 6 2.57E-4 0.01
12 SGTC_1991 st073000 3.29 8.9 µM 2.20E-4 NEO1 25 4.94E-4 0.00
13 SGTC_705 1124-0212 3.28 310.0 µM 2.35E-4 16 5.24E-4 0.10
14 SGTC_2285 7945142 3.24 200.0 µM 2.73E-4 16 5.98E-4 0.06
15 SGTC_210 3551-0445 3.18 56.8 µM 3.45E-4 22 7.36E-4 0.09
16 SGTC_815 1582-0027 3.12 36.9 µM 4.40E-4 14 9.14E-4 0.03
17 SGTC_2929 9013348 3.12 24.6 µM 4.40E-4 7 9.14E-4 0.16
18 SGTC_2165 5727197 3.11 32.1 µM 4.53E-4 4 9.38E-4 0.09
19 SGTC_1371 2421-0008 3.11 32.7 µM 4.58E-4 mitochondrial processes 38 9.48E-4 0.01
20 SGTC_3326 9140064 3.09 16.4 µM 4.85E-4 12 9.96E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.099 8.15E-9 YNL092W_p YNL092W_p hom Putative S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; YNL092W is not an essential gene
0.097 1.68E-8 YLR209C PNP1 hom Purine nucleoside phosphorylase, specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway
0.093 7.43E-8 YJR098C_p YJR098C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.092 9.57E-8 YAR008W SEN34 het Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
0.090 2.04E-7 YDL052C SLC1 hom 1-acyl-sn-glycerol-3-phosphate acyltransferase, catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes
0.088 3.28E-7 YGL245W GUS1 het Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress
0.087 4.08E-7 YMR235C RNA1 het GTPase activating protein (GAP) for Gsp1p, involved in nuclear transport
0.086 6.68E-7 YGL074C_d YGL074C_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
0.081 2.33E-6 YMR016C SOK2 hom Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; SOK2 has a paralog, PHD1, that arose from the whole genome duplication
0.080 3.18E-6 YIL078W THS1 het Threonyl-tRNA synthetase, essential cytoplasmic protein
0.076 1.04E-5 YDR130C FIN1 hom Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress
0.076 1.07E-5 YER150W SPI1 hom GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; similar to Sed1p
0.076 1.18E-5 YFR035C_p YFR035C_p hom Putative protein of unknown function, deletion mutant exhibits synthetic phenotype with alpha-synuclein
0.074 1.59E-5 YJR110W YMR1 hom Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family
0.074 1.64E-5 YPL209C IPL1 het Aurora kinase subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), involved in regulating kinetochore-microtubule attachments; helps maintain condensed chromosomes during anaphase and early telophase; required for SPB cohesion and prevention of multipolar spindle formation; localizes to nuclear foci that become diffuse upon DNA replication stress; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon SAC arrest