YLR180W / SAM1

S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR180W / SAM1

Click on Significant Values for Screen Details ID:SGTC_8|Compound:0354-0001|FD-Score:4.91|P-value:1.35E-7 ID:SGTC_59|Compound:0330-0160|FD-Score:2.98|P-value:8.99E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:3.26|P-value:3.13E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:-3.29|P-value:3.14E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:3.61|P-value:7.78E-5 ID:SGTC_318|Compound:1488-0629|FD-Score:3.04|P-value:7.11E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:3.07|P-value:6.55E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:-4.51|P-value:1.37E-6 ID:SGTC_710|Compound:3448-4187|FD-Score:3.84|P-value:2.83E-5 ID:SGTC_777|Compound:1315-0167|FD-Score:-3.13|P-value:5.75E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:-3.28|P-value:3.27E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:-3.62|P-value:8.34E-5 ID:SGTC_1184|Compound:1269-1718|FD-Score:5.40|P-value:8.04E-9 ID:SGTC_1273|Compound:0849-0096|FD-Score:3.24|P-value:3.39E-4 ID:SGTC_1274|Compound:0865-0114|FD-Score:3.77|P-value:3.86E-5 ID:SGTC_1419|Compound:3993-2473|FD-Score:3.55|P-value:1.01E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:5.10|P-value:4.71E-8 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.71|P-value:5.01E-5 ID:SGTC_1682|Compound:st012968|FD-Score:-3.06|P-value:7.29E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.30|P-value:2.70E-4 ID:SGTC_1866|Compound:st057638|FD-Score:2.98|P-value:8.85E-4 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:-3.16|P-value:5.13E-4 ID:SGTC_1937|Compound:st074714|FD-Score:-3.28|P-value:3.27E-4 ID:SGTC_2157|Compound:5649910|FD-Score:2.97|P-value:9.17E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:-3.27|P-value:3.37E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:3.76|P-value:4.19E-5 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.11|P-value:5.49E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-3.21|P-value:4.31E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.87|P-value:2.48E-5 ID:SGTC_2698|Compound:st077697|FD-Score:-3.31|P-value:2.89E-4 ID:SGTC_2823|Compound:7987624|FD-Score:3.49|P-value:1.27E-4 ID:SGTC_2929|Compound:9013348|FD-Score:3.87|P-value:2.51E-5 ID:SGTC_2934|Compound:9036370|FD-Score:5.40|P-value:7.86E-9 ID:SGTC_3008|Compound:9078912|FD-Score:-3.52|P-value:1.29E-4 ID:SGTC_3009|Compound:9080819|FD-Score:3.13|P-value:5.22E-4 ID:SGTC_3015|Compound:9080845|FD-Score:3.33|P-value:2.37E-4 ID:SGTC_3144|Compound:9087057|FD-Score:3.55|P-value:9.84E-5 ID:SGTC_3338|Compound:9144300|FD-Score:2.96|P-value:9.59E-4 ID:SGTC_8|Compound:0354-0001|FD-Score:4.91|P-value:1.35E-7 ID:SGTC_59|Compound:0330-0160|FD-Score:2.98|P-value:8.99E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:3.26|P-value:3.13E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:-3.29|P-value:3.14E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:3.61|P-value:7.78E-5 ID:SGTC_318|Compound:1488-0629|FD-Score:3.04|P-value:7.11E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:3.07|P-value:6.55E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:-4.51|P-value:1.37E-6 ID:SGTC_710|Compound:3448-4187|FD-Score:3.84|P-value:2.83E-5 ID:SGTC_777|Compound:1315-0167|FD-Score:-3.13|P-value:5.75E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:-3.28|P-value:3.27E-4 ID:SGTC_1181|Compound:1222-0449|FD-Score:-3.62|P-value:8.34E-5 ID:SGTC_1184|Compound:1269-1718|FD-Score:5.40|P-value:8.04E-9 ID:SGTC_1273|Compound:0849-0096|FD-Score:3.24|P-value:3.39E-4 ID:SGTC_1274|Compound:0865-0114|FD-Score:3.77|P-value:3.86E-5 ID:SGTC_1419|Compound:3993-2473|FD-Score:3.55|P-value:1.01E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:5.10|P-value:4.71E-8 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:3.71|P-value:5.01E-5 ID:SGTC_1682|Compound:st012968|FD-Score:-3.06|P-value:7.29E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.30|P-value:2.70E-4 ID:SGTC_1866|Compound:st057638|FD-Score:2.98|P-value:8.85E-4 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:-3.16|P-value:5.13E-4 ID:SGTC_1937|Compound:st074714|FD-Score:-3.28|P-value:3.27E-4 ID:SGTC_2157|Compound:5649910|FD-Score:2.97|P-value:9.17E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:-3.27|P-value:3.37E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:3.76|P-value:4.19E-5 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.11|P-value:5.49E-4 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-3.21|P-value:4.31E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:3.87|P-value:2.48E-5 ID:SGTC_2698|Compound:st077697|FD-Score:-3.31|P-value:2.89E-4 ID:SGTC_2823|Compound:7987624|FD-Score:3.49|P-value:1.27E-4 ID:SGTC_2929|Compound:9013348|FD-Score:3.87|P-value:2.51E-5 ID:SGTC_2934|Compound:9036370|FD-Score:5.40|P-value:7.86E-9 ID:SGTC_3008|Compound:9078912|FD-Score:-3.52|P-value:1.29E-4 ID:SGTC_3009|Compound:9080819|FD-Score:3.13|P-value:5.22E-4 ID:SGTC_3015|Compound:9080845|FD-Score:3.33|P-value:2.37E-4 ID:SGTC_3144|Compound:9087057|FD-Score:3.55|P-value:9.84E-5 ID:SGTC_3338|Compound:9144300|FD-Score:2.96|P-value:9.59E-4

Top fitness defect scores for YLR180W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2934 9036370 5.40 36.9 µM 7.86E-9 14 3.26E-8
2 SGTC_1184 1269-1718 5.40 139.0 µM 8.04E-9 8 3.33E-8
3 SGTC_1456 k015-0012 5.10 13.0 µM 4.71E-8 9 1.69E-7
4 SGTC_8 0354-0001 4.91 70.0 µM 1.35E-7 47 4.46E-7
5 SGTC_2651 1327-0036 3.87 5.0 µM 2.48E-5 163 5.36E-5
6 SGTC_2929 9013348 3.87 24.6 µM 2.51E-5 23 5.42E-5
7 SGTC_710 3448-4187 3.84 58.0 µM 2.83E-5 fatty acid desaturase (OLE1) 64 6.04E-5
8 SGTC_1274 0865-0114 3.77 173.0 µM 3.86E-5 26 8.04E-5
9 SGTC_2536 dehydroabietamide 3.76 32.0 µM 4.19E-5 43 8.66E-5
10 SGTC_1565 sinapyl alcohol 3.71 95.1 µM 5.01E-5 37 1.02E-4
11 SGTC_245 methotrexate 3.61 289.6 µM 7.78E-5 52 1.53E-4
12 SGTC_3144 9087057 3.55 49.5 µM 9.84E-5 24 1.90E-4
13 SGTC_1419 3993-2473 3.55 62.6 µM 1.01E-4 28 1.94E-4
14 SGTC_2823 7987624 3.49 58.4 µM 1.27E-4 49 2.40E-4
15 SGTC_3015 9080845 3.33 49.5 µM 2.37E-4 23 4.27E-4
16 SGTC_1787 fluconazole 3.30 80.0 µM 2.70E-4 iron homeostasis 66 4.80E-4
17 SGTC_103 0844-0104 3.26 51.8 µM 3.13E-4 ERAD & cell cycle 183 5.50E-4
18 SGTC_1273 0849-0096 3.24 14.1 µM 3.39E-4 RPP1 & pyrimidine depletion 44 5.92E-4
19 SGTC_3009 9080819 3.13 71.4 µM 5.22E-4 51 8.82E-4
20 SGTC_2583 asiatic acid 3.11 100.0 µM 5.49E-4 67 9.24E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.186 1.72E-27 YGR055W MUP1 hom High affinity methionine permease, integral membrane protein with 13 putative membrane-spanning regions; also involved in cysteine uptake
0.141 1.91E-16 YMR070W MOT3 hom Transcriptional repressor and activator with two C2-H2 zinc fingers; involved in repression of a subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, and repression of ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of the Tup1p-Cyc8p general repressor to promoters; involved in positive transcriptional regulation of CWP2 and other genes; can form the [MOT3+] prion
0.130 4.65E-14 YIL135C VHS2 hom Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p
0.117 8.48E-12 YNL239W LAP3 hom Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH
0.115 2.09E-11 YFL037W TUB2 het Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.106 7.10E-10 YIL052C RPL34B hom Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
0.100 6.22E-9 YNL065W AQR1 hom Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
0.098 1.31E-8 YJL019W MPS3 het Nuclear envelope protein required for SPB insertion; initiation of SPB duplication and nuclear fusion; acetylation of Mps3p by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and at telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; N-terminal acetylated by Eco1p
0.096 2.29E-8 YDR321W ASP1 hom Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively
0.095 3.82E-8 YEL007W MIT1 hom Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
0.094 5.06E-8 YOL037C_d YOL037C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W
0.094 5.37E-8 YLR184W_d YLR184W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.093 6.82E-8 YLR436C ECM30 hom Putative protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm
0.092 8.16E-8 YLL048C YBT1 hom Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
0.090 1.89E-7 YBR042C CST26 hom Putative transferase involved in phospholipid biosynthesis; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication