YLR186W / EMG1

Methyltransferase for rRNA; catalyzes methylation of the pseudouridine residue 1191 of 18S rRNA; member of the SPOUT methyltransferase family; required for maturation of 18S rRNA and for 40S ribosomal subunit production independently of methyltransferase activity; forms homodimers; human ortholog is mutated in Bowen-Conradi syndrome, and the equivalent mutation in yeast affects Emg1p dimerization and localization but not its methyltransferase activity

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR186W / EMG1

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:3.51|P-value:4.11E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:3.45|P-value:5.17E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:4.29|P-value:2.11E-5 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:-4.20|P-value:2.48E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:6.49|P-value:2.79E-10 ID:SGTC_376|Compound:3970-0862|FD-Score:-3.32|P-value:6.47E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:-4.00|P-value:5.57E-5 ID:SGTC_384|Compound:k007-0144|FD-Score:3.25|P-value:9.94E-4 ID:SGTC_856|Compound:0433-0016|FD-Score:3.27|P-value:9.18E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:-6.58|P-value:1.16E-10 ID:SGTC_1248|Compound:0469-0687|FD-Score:-3.63|P-value:2.26E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:-4.28|P-value:1.79E-5 ID:SGTC_1466|Compound:k213-0079|FD-Score:3.26|P-value:9.51E-4 ID:SGTC_1766|Compound:st045414|FD-Score:3.39|P-value:6.20E-4 ID:SGTC_1935|Compound:st074532|FD-Score:-3.60|P-value:2.53E-4 ID:SGTC_2068|Compound:5226020|FD-Score:3.63|P-value:2.74E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.43|P-value:5.42E-4 ID:SGTC_2204|Compound:7233102|FD-Score:-3.48|P-value:3.85E-4 ID:SGTC_2808|Compound:7946468|FD-Score:-3.43|P-value:4.51E-4 ID:SGTC_2809|Compound:7964133|FD-Score:-5.79|P-value:1.17E-8 ID:SGTC_2839|Compound:9003951|FD-Score:-4.00|P-value:5.45E-5 ID:SGTC_2894|Compound:9048625|FD-Score:3.99|P-value:7.16E-5 ID:SGTC_2932|Compound:9016711|FD-Score:-4.25|P-value:1.97E-5 ID:SGTC_2935|Compound:9038016|FD-Score:-9.54|P-value:2.01E-20 ID:SGTC_2940|Compound:9060638|FD-Score:-3.25|P-value:8.30E-4 ID:SGTC_2962|Compound:9082311|FD-Score:3.37|P-value:6.63E-4 ID:SGTC_3069|Compound:9117009|FD-Score:-3.34|P-value:6.17E-4 ID:SGTC_3314|Compound:9135860|FD-Score:-3.25|P-value:8.21E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:3.51|P-value:4.11E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:3.45|P-value:5.17E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:4.29|P-value:2.11E-5 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:-4.20|P-value:2.48E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:6.49|P-value:2.79E-10 ID:SGTC_376|Compound:3970-0862|FD-Score:-3.32|P-value:6.47E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:-4.00|P-value:5.57E-5 ID:SGTC_384|Compound:k007-0144|FD-Score:3.25|P-value:9.94E-4 ID:SGTC_856|Compound:0433-0016|FD-Score:3.27|P-value:9.18E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:-6.58|P-value:1.16E-10 ID:SGTC_1248|Compound:0469-0687|FD-Score:-3.63|P-value:2.26E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:-4.28|P-value:1.79E-5 ID:SGTC_1466|Compound:k213-0079|FD-Score:3.26|P-value:9.51E-4 ID:SGTC_1766|Compound:st045414|FD-Score:3.39|P-value:6.20E-4 ID:SGTC_1935|Compound:st074532|FD-Score:-3.60|P-value:2.53E-4 ID:SGTC_2068|Compound:5226020|FD-Score:3.63|P-value:2.74E-4 ID:SGTC_2079|Compound:5212404|FD-Score:3.43|P-value:5.42E-4 ID:SGTC_2204|Compound:7233102|FD-Score:-3.48|P-value:3.85E-4 ID:SGTC_2808|Compound:7946468|FD-Score:-3.43|P-value:4.51E-4 ID:SGTC_2809|Compound:7964133|FD-Score:-5.79|P-value:1.17E-8 ID:SGTC_2839|Compound:9003951|FD-Score:-4.00|P-value:5.45E-5 ID:SGTC_2894|Compound:9048625|FD-Score:3.99|P-value:7.16E-5 ID:SGTC_2932|Compound:9016711|FD-Score:-4.25|P-value:1.97E-5 ID:SGTC_2935|Compound:9038016|FD-Score:-9.54|P-value:2.01E-20 ID:SGTC_2940|Compound:9060638|FD-Score:-3.25|P-value:8.30E-4 ID:SGTC_2962|Compound:9082311|FD-Score:3.37|P-value:6.63E-4 ID:SGTC_3069|Compound:9117009|FD-Score:-3.34|P-value:6.17E-4 ID:SGTC_3314|Compound:9135860|FD-Score:-3.25|P-value:8.21E-4

Top fitness defect scores for YLR186W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_227 fluconazole 6.49 33.5 µM 2.79E-10 azole & statin 25 4.31E-11 0.19
2 SGTC_112 phenothiazine 4.29 287.2 µM 2.11E-5 unfolded protein response 4 8.77E-6 0.41
3 SGTC_2894 9048625 3.99 32.5 µM 7.16E-5 7 3.31E-5 0.00
4 SGTC_2068 5226020 3.63 147.0 µM 2.74E-4 10 1.43E-4 0.14
5 SGTC_7 0192-1582 3.51 4.2 µM 4.11E-4 7 2.21E-4 0.11
6 SGTC_103 0844-0104 3.45 51.8 µM 5.17E-4 ERAD & cell cycle 60 2.84E-4 1.30E-4
7 SGTC_2079 5212404 3.43 121.0 µM 5.42E-4 4 2.99E-4 0.14
8 SGTC_1766 st045414 3.39 44.8 µM 6.20E-4 RPP1 & pyrimidine depletion 17 3.46E-4 0.02
9 SGTC_2962 9082311 3.37 13.2 µM 6.63E-4 20 3.72E-4 0.15
10 SGTC_856 0433-0016 3.27 11.3 µM 9.18E-4 9 5.31E-4 0.13
11 SGTC_1466 k213-0079 3.26 57.5 µM 9.51E-4 21 5.51E-4 0.08
12 SGTC_384 k007-0144 3.25 5.2 µM 9.94E-4 ERAD & cell cycle 23 5.78E-4 0.01
13 SGTC_1204 4049-0315 3.21 18.3 µM 0.00114 16 6.69E-4 0.01
14 SGTC_1298 1079-1278 3.13 263.0 µM 0.00144 DNA damage response 11 8.67E-4 0.21
15 SGTC_1747 st037496 3.12 66.6 µM 0.00152 20 9.19E-4 0.07
16 SGTC_2257 7960413 3.04 200.0 µM 0.00194 NEO1-PIK1 31 0.00120 0.07
17 SGTC_1198 1612-1005 2.99 86.7 µM 0.00220 11 0.00137 0.01
18 SGTC_2049 5262831 2.98 67.2 µM 0.00228 23 0.00142 0.03
19 SGTC_352 k048-0007 2.94 5.1 µM 0.00263 ERAD & cell cycle 27 0.00166 0.02
20 SGTC_1038 k292-0204 2.92 154.0 µM 0.00279 17 0.00178 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.176 1.16E-24 YMR040W YET2 hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein
0.141 2.40E-16 YCR093W CDC39 het Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
0.140 3.67E-16 YOR174W MED4 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.137 1.79E-15 YDR143C SAN1 hom Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition
0.136 2.84E-15 YJL195C_d YJL195C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
0.126 2.23E-13 YMR031C EIS1 hom Component of the eisosome required for proper eisosome assembly; similarity to Ykl050cp and Uso1p; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress
0.125 3.58E-13 YDR206W EBS1 hom Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors
0.124 4.81E-13 YMR041C ARA2 hom NAD-dependent arabinose dehydrogenase, involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase
0.124 6.18E-13 YPR177C_d YPR177C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the 5' end of the essential PRP4 gene encoding a component of the U4/U6-U5 snRNP complex
0.117 9.58E-12 YEL040W UTR2 hom Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck
0.110 1.49E-10 YPL037C EGD1 hom Subunit beta1 of the nascent polypeptide-associated complex (NAC) involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b
0.106 6.84E-10 YKL197C PEX1 hom AAA-peroxin that heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis
0.103 2.01E-9 YBR220C_p YBR220C_p hom Putative protein of unknown function; YBR220C is not an essential gene
0.101 4.19E-9 YMR052C-A_d YMR052C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.099 1.03E-8 YOR354C MSC6 hom Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies