YLR196W / PWP1

Protein with WD-40 repeats involved in rRNA processing; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR196W / PWP1

Click on Significant Values for Screen Details ID:SGTC_344|Compound:k064-0035|FD-Score:5.10|P-value:2.46E-9 ID:SGTC_348|Compound:k064-0012|FD-Score:3.79|P-value:6.82E-6 ID:SGTC_351|Compound:caspofungin|FD-Score:-3.78|P-value:6.24E-6 ID:SGTC_356|Compound:1634-0012|FD-Score:2.88|P-value:4.86E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:-3.19|P-value:1.12E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:2.76|P-value:7.92E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:3.50|P-value:3.02E-5 ID:SGTC_507|Compound:nimesulide|FD-Score:3.37|P-value:5.47E-5 ID:SGTC_646|Compound:4092-0839|FD-Score:3.85|P-value:5.14E-6 ID:SGTC_696|Compound:1560-0013|FD-Score:2.85|P-value:5.50E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.51|P-value:2.44E-5 ID:SGTC_1143|Compound:3454-2408|FD-Score:3.23|P-value:1.06E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:4.19|P-value:7.66E-7 ID:SGTC_1265|Compound:0819-1001|FD-Score:3.96|P-value:2.76E-6 ID:SGTC_1360|Compound:1598-0022|FD-Score:4.37|P-value:2.58E-7 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.31|P-value:7.38E-5 ID:SGTC_1460|Compound:k062-0059|FD-Score:2.82|P-value:6.12E-4 ID:SGTC_1800|Compound:st050079|FD-Score:-3.06|P-value:1.99E-4 ID:SGTC_1813|Compound:st051246|FD-Score:2.70|P-value:9.83E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:-2.80|P-value:5.96E-4 ID:SGTC_1892|Compound:st058411|FD-Score:-3.11|P-value:1.65E-4 ID:SGTC_1947|Compound:st076332|FD-Score:-3.64|P-value:1.31E-5 ID:SGTC_1953|Compound:st076800|FD-Score:-4.20|P-value:6.06E-7 ID:SGTC_1990|Compound:st072050|FD-Score:-2.72|P-value:8.10E-4 ID:SGTC_2162|Compound:5663267|FD-Score:3.34|P-value:6.46E-5 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:-3.33|P-value:5.85E-5 ID:SGTC_2178|Compound:5812956|FD-Score:-3.19|P-value:1.13E-4 ID:SGTC_2185|Compound:6113692|FD-Score:3.74|P-value:9.05E-6 ID:SGTC_2238|Compound:6671620|FD-Score:-3.30|P-value:6.86E-5 ID:SGTC_2240|Compound:6690706|FD-Score:2.85|P-value:5.57E-4 ID:SGTC_2316|Compound:5844804|FD-Score:5.44|P-value:2.04E-10 ID:SGTC_2325|Compound:6158266|FD-Score:-3.39|P-value:4.49E-5 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:2.71|P-value:9.48E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.09|P-value:1.74E-4 ID:SGTC_2803|Compound:7707263|FD-Score:-2.75|P-value:7.41E-4 ID:SGTC_2806|Compound:7952793|FD-Score:-3.87|P-value:3.85E-6 ID:SGTC_2813|Compound:7971799|FD-Score:6.16|P-value:7.69E-13 ID:SGTC_2894|Compound:9048625|FD-Score:5.52|P-value:1.19E-10 ID:SGTC_2899|Compound:9053889|FD-Score:3.04|P-value:2.49E-4 ID:SGTC_2959|Compound:9079155|FD-Score:-2.67|P-value:9.85E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-3.01|P-value:2.50E-4 ID:SGTC_3315|Compound:9136395|FD-Score:4.48|P-value:1.39E-7 ID:SGTC_344|Compound:k064-0035|FD-Score:5.10|P-value:2.46E-9 ID:SGTC_348|Compound:k064-0012|FD-Score:3.79|P-value:6.82E-6 ID:SGTC_351|Compound:caspofungin|FD-Score:-3.78|P-value:6.24E-6 ID:SGTC_356|Compound:1634-0012|FD-Score:2.88|P-value:4.86E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:-3.19|P-value:1.12E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:2.76|P-value:7.92E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:3.50|P-value:3.02E-5 ID:SGTC_507|Compound:nimesulide|FD-Score:3.37|P-value:5.47E-5 ID:SGTC_646|Compound:4092-0839|FD-Score:3.85|P-value:5.14E-6 ID:SGTC_696|Compound:1560-0013|FD-Score:2.85|P-value:5.50E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.51|P-value:2.44E-5 ID:SGTC_1143|Compound:3454-2408|FD-Score:3.23|P-value:1.06E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:4.19|P-value:7.66E-7 ID:SGTC_1265|Compound:0819-1001|FD-Score:3.96|P-value:2.76E-6 ID:SGTC_1360|Compound:1598-0022|FD-Score:4.37|P-value:2.58E-7 ID:SGTC_1453|Compound:4237-0541|FD-Score:3.31|P-value:7.38E-5 ID:SGTC_1460|Compound:k062-0059|FD-Score:2.82|P-value:6.12E-4 ID:SGTC_1800|Compound:st050079|FD-Score:-3.06|P-value:1.99E-4 ID:SGTC_1813|Compound:st051246|FD-Score:2.70|P-value:9.83E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:-2.80|P-value:5.96E-4 ID:SGTC_1892|Compound:st058411|FD-Score:-3.11|P-value:1.65E-4 ID:SGTC_1947|Compound:st076332|FD-Score:-3.64|P-value:1.31E-5 ID:SGTC_1953|Compound:st076800|FD-Score:-4.20|P-value:6.06E-7 ID:SGTC_1990|Compound:st072050|FD-Score:-2.72|P-value:8.10E-4 ID:SGTC_2162|Compound:5663267|FD-Score:3.34|P-value:6.46E-5 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:-3.33|P-value:5.85E-5 ID:SGTC_2178|Compound:5812956|FD-Score:-3.19|P-value:1.13E-4 ID:SGTC_2185|Compound:6113692|FD-Score:3.74|P-value:9.05E-6 ID:SGTC_2238|Compound:6671620|FD-Score:-3.30|P-value:6.86E-5 ID:SGTC_2240|Compound:6690706|FD-Score:2.85|P-value:5.57E-4 ID:SGTC_2316|Compound:5844804|FD-Score:5.44|P-value:2.04E-10 ID:SGTC_2325|Compound:6158266|FD-Score:-3.39|P-value:4.49E-5 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:2.71|P-value:9.48E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.09|P-value:1.74E-4 ID:SGTC_2803|Compound:7707263|FD-Score:-2.75|P-value:7.41E-4 ID:SGTC_2806|Compound:7952793|FD-Score:-3.87|P-value:3.85E-6 ID:SGTC_2813|Compound:7971799|FD-Score:6.16|P-value:7.69E-13 ID:SGTC_2894|Compound:9048625|FD-Score:5.52|P-value:1.19E-10 ID:SGTC_2899|Compound:9053889|FD-Score:3.04|P-value:2.49E-4 ID:SGTC_2959|Compound:9079155|FD-Score:-2.67|P-value:9.85E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-3.01|P-value:2.50E-4 ID:SGTC_3315|Compound:9136395|FD-Score:4.48|P-value:1.39E-7

Top fitness defect scores for YLR196W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2813 7971799 6.16 71.4 µM 7.69E-13 1 3.73E-10 0.99
2 SGTC_2894 9048625 5.52 32.5 µM 1.19E-10 2 1.74E-8 0.55
3 SGTC_2316 5844804 5.44 197.4 µM 2.04E-10 4 2.61E-8 0.05
4 SGTC_344 k064-0035 5.10 6.4 µM 2.46E-9 60S ribosome export 3 1.74E-7 0.56
5 SGTC_3315 9136395 4.48 68.5 µM 1.39E-7 4 3.80E-6 0.03
6 SGTC_1360 1598-0022 4.37 39.8 µM 2.58E-7 ERG2 5 6.08E-6 0.12
7 SGTC_1248 0469-0687 4.19 58.6 µM 7.66E-7 8 1.40E-5 0.47
8 SGTC_1265 0819-1001 3.96 68.8 µM 2.76E-6 7 3.73E-5 0.02
9 SGTC_646 4092-0839 3.85 27.3 µM 5.14E-6 plasma membrane duress 10 6.01E-5 0.18
10 SGTC_348 k064-0012 3.79 10.7 µM 6.82E-6 60S ribosome export 7 7.47E-5 0.02
11 SGTC_2185 6113692 3.74 200.0 µM 9.05E-6 ergosterol biosynthesis 7 9.28E-5 0.08
12 SGTC_452 diindolylmethane 3.50 8.0 µM 3.02E-5 RNA processing & uracil transport 7 2.34E-4 0.00
13 SGTC_507 nimesulide 3.37 162.0 µM 5.47E-5 6 3.70E-4 0.07
14 SGTC_2162 5663267 3.34 200.0 µM 6.46E-5 17 4.20E-4 0.09
15 SGTC_1453 4237-0541 3.31 195.0 µM 7.38E-5 heme biosynthesis & mitochondrial translocase 22 4.66E-4 0.04
16 SGTC_1143 3454-2408 3.23 5.1 µM 1.06E-4 60S ribosome export 7 6.16E-4 0.01
17 SGTC_2899 9053889 3.04 58.4 µM 2.49E-4 6 0.00119 0.03
18 SGTC_356 1634-0012 2.88 32.5 µM 4.86E-4 16 0.00199 0.01
19 SGTC_696 1560-0013 2.85 611.0 µM 5.50E-4 27 0.00219 0.06
20 SGTC_2240 6690706 2.85 185.9 µM 5.57E-4 16 0.00222 0.08

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.115 2.63E-11 YGR019W UGA1 hom Gamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress
0.107 6.03E-10 YGR185C TYS1 het Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress
0.103 2.09E-9 YMR139W RIM11 hom Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress
0.103 2.23E-9 YNR070W_p PDR18_p hom Putative transporter of the ATP-binding cassette (ABC) family, implicated in pleiotropic drug resistance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.099 8.90E-9 YKL108W SLD2 het Single-stranded DNA origin-binding and annealing protein; required for the initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; unphosphorylated or CDK-phosphorylated Sld2p binds to the MCM2-7 complex;required for the S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress
0.099 9.32E-9 YGR248W SOL4 hom 6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication
0.096 2.81E-8 YGL131C SNT2 hom DNA binding protein with similarity to the S. pombe Snt2 protein; RING finger ubiquitin ligase (E3) involved in ubiquitylation and degradation of excess histones; interacts with Ubc4p; null mutant sensitive to hydroxyurea (HU); computational analysis suggests that Snt2p is a transcription factor with a role in regulation of expression of genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.095 3.34E-8 YJR010W MET3 hom ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism
0.093 6.98E-8 YDR339C FCF1 het Putative PINc domain nuclease required for early cleavages of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p
0.092 9.41E-8 YOL112W MSB4 hom GTPase-activating protein of the Ras superfamily that acts primarily on Sec4p, localizes to the bud site and bud tip, has similarity to Msb3p; msb3 msb4 double mutation causes defects in secretion and actin organization
0.092 1.08E-7 YBR207W FTH1 hom Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.090 1.96E-7 YDL086W_p YDL086W_p hom Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene
0.087 4.91E-7 YBR084W MIS1 hom Mitochondrial C1-tetrahydrofolate synthase, involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase
0.086 6.22E-7 YDR387C_p YDR387C_p hom Putative transporter, member of the sugar porter family; YDR387C is not an essential gene
0.085 7.51E-7 YGR009C SEC9 het t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog