YLR199C / PBA1

Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR199C / PBA1

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:7.86|P-value:6.19E-21 ID:SGTC_65|Compound:0798-0905|FD-Score:4.40|P-value:9.33E-8 ID:SGTC_67|Compound:0335-0847|FD-Score:6.38|P-value:2.12E-14 ID:SGTC_73|Compound:0327-0325|FD-Score:2.77|P-value:5.28E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.25|P-value:5.92E-5 ID:SGTC_368|Compound:fentichlor|FD-Score:-2.73|P-value:6.02E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:-2.91|P-value:2.83E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:-3.45|P-value:2.22E-5 ID:SGTC_439|Compound:amiodarone|FD-Score:-4.00|P-value:1.11E-6 ID:SGTC_463|Compound:ikarugamyin|FD-Score:2.87|P-value:3.41E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:-2.89|P-value:3.12E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.18|P-value:8.21E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:2.69|P-value:7.23E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:3.39|P-value:2.97E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.48|P-value:1.92E-5 ID:SGTC_755|Compound:1995-0008|FD-Score:-2.68|P-value:7.54E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:-3.44|P-value:2.25E-5 ID:SGTC_909|Compound:3448-9333|FD-Score:-3.26|P-value:5.65E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-2.77|P-value:5.24E-4 ID:SGTC_1138|Compound:3761-0006|FD-Score:2.81|P-value:4.35E-4 ID:SGTC_1332|Compound:1480-0653|FD-Score:2.74|P-value:5.86E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:3.52|P-value:1.57E-5 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.10|P-value:1.19E-4 ID:SGTC_1460|Compound:k062-0059|FD-Score:-3.00|P-value:1.91E-4 ID:SGTC_1526|Compound:st051518|FD-Score:-3.47|P-value:1.98E-5 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:-3.43|P-value:2.44E-5 ID:SGTC_1826|Compound:st055655|FD-Score:-2.68|P-value:7.38E-4 ID:SGTC_1832|Compound:st054113|FD-Score:3.45|P-value:2.23E-5 ID:SGTC_1839|Compound:st053978|FD-Score:-2.74|P-value:5.93E-4 ID:SGTC_2050|Compound:5263056|FD-Score:2.63|P-value:9.34E-4 ID:SGTC_2060|Compound:5238662|FD-Score:2.88|P-value:3.25E-4 ID:SGTC_2168|Compound:5727979|FD-Score:-2.73|P-value:6.01E-4 ID:SGTC_2361|Compound:9034121|FD-Score:2.72|P-value:6.33E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:2.75|P-value:5.74E-4 ID:SGTC_2602|Compound:kawain|FD-Score:2.96|P-value:2.25E-4 ID:SGTC_2966|Compound:9084041|FD-Score:-2.97|P-value:2.15E-4 ID:SGTC_3202|Compound:9113761|FD-Score:2.95|P-value:2.41E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-5.92|P-value:1.19E-12 ID:SGTC_3315|Compound:9136395|FD-Score:2.64|P-value:8.79E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:7.86|P-value:6.19E-21 ID:SGTC_65|Compound:0798-0905|FD-Score:4.40|P-value:9.33E-8 ID:SGTC_67|Compound:0335-0847|FD-Score:6.38|P-value:2.12E-14 ID:SGTC_73|Compound:0327-0325|FD-Score:2.77|P-value:5.28E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.25|P-value:5.92E-5 ID:SGTC_368|Compound:fentichlor|FD-Score:-2.73|P-value:6.02E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:-2.91|P-value:2.83E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:-3.45|P-value:2.22E-5 ID:SGTC_439|Compound:amiodarone|FD-Score:-4.00|P-value:1.11E-6 ID:SGTC_463|Compound:ikarugamyin|FD-Score:2.87|P-value:3.41E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:-2.89|P-value:3.12E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.18|P-value:8.21E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:2.69|P-value:7.23E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:3.39|P-value:2.97E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.48|P-value:1.92E-5 ID:SGTC_755|Compound:1995-0008|FD-Score:-2.68|P-value:7.54E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:-3.44|P-value:2.25E-5 ID:SGTC_909|Compound:3448-9333|FD-Score:-3.26|P-value:5.65E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-2.77|P-value:5.24E-4 ID:SGTC_1138|Compound:3761-0006|FD-Score:2.81|P-value:4.35E-4 ID:SGTC_1332|Compound:1480-0653|FD-Score:2.74|P-value:5.86E-4 ID:SGTC_1450|Compound:4112-4260|FD-Score:3.52|P-value:1.57E-5 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.10|P-value:1.19E-4 ID:SGTC_1460|Compound:k062-0059|FD-Score:-3.00|P-value:1.91E-4 ID:SGTC_1526|Compound:st051518|FD-Score:-3.47|P-value:1.98E-5 ID:SGTC_1582|Compound:3',3',6'-trihydroxyflavone|FD-Score:-3.43|P-value:2.44E-5 ID:SGTC_1826|Compound:st055655|FD-Score:-2.68|P-value:7.38E-4 ID:SGTC_1832|Compound:st054113|FD-Score:3.45|P-value:2.23E-5 ID:SGTC_1839|Compound:st053978|FD-Score:-2.74|P-value:5.93E-4 ID:SGTC_2050|Compound:5263056|FD-Score:2.63|P-value:9.34E-4 ID:SGTC_2060|Compound:5238662|FD-Score:2.88|P-value:3.25E-4 ID:SGTC_2168|Compound:5727979|FD-Score:-2.73|P-value:6.01E-4 ID:SGTC_2361|Compound:9034121|FD-Score:2.72|P-value:6.33E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:2.75|P-value:5.74E-4 ID:SGTC_2602|Compound:kawain|FD-Score:2.96|P-value:2.25E-4 ID:SGTC_2966|Compound:9084041|FD-Score:-2.97|P-value:2.15E-4 ID:SGTC_3202|Compound:9113761|FD-Score:2.95|P-value:2.41E-4 ID:SGTC_3206|Compound:9114350|FD-Score:-5.92|P-value:1.19E-12 ID:SGTC_3315|Compound:9136395|FD-Score:2.64|P-value:8.79E-4

Top fitness defect scores for YLR199C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_64 000l-0018 7.86 272.1 µM 6.19E-21 ubiquinone biosynthesis & proteasome 52 1.86E-15
2 SGTC_67 0335-0847 6.38 266.0 µM 2.12E-14 ubiquinone biosynthesis & proteasome 61 8.98E-11
3 SGTC_65 0798-0905 4.40 35.0 µM 9.33E-8 calcium & mitochondrial duress 67 5.39E-6
4 SGTC_1450 4112-4260 3.52 37.6 µM 1.57E-5 calcium & mitochondrial duress 42 2.20E-4
5 SGTC_1832 st054113 3.45 55.0 µM 2.23E-5 13 2.84E-4
6 SGTC_726 3448-4391 3.39 34.2 µM 2.97E-5 33 3.49E-4
7 SGTC_2602 kawain 2.96 100.0 µM 2.25E-4 80 0.00152
8 SGTC_3202 9113761 2.95 49.5 µM 2.41E-4 109 0.00161
9 SGTC_2060 5238662 2.88 140.0 µM 3.25E-4 69 0.00200
10 SGTC_463 ikarugamyin 2.87 104.0 µM 3.41E-4 19 0.00207
11 SGTC_1138 3761-0006 2.81 1.0 µM 4.35E-4 35 0.00247
12 SGTC_73 0327-0325 2.77 16.2 µM 5.28E-4 DNA damage response 146 0.00284
13 SGTC_2595 hexamethylquercetagetin 2.75 100.0 µM 5.74E-4 91 0.00302
14 SGTC_1332 1480-0653 2.74 17.5 µM 5.86E-4 TSC3-RPN4 82 0.00307
15 SGTC_2361 9034121 2.72 116.0 µM 6.33E-4 RSC complex & mRNA processing 78 0.00325
16 SGTC_669 0108-0021 2.69 26.2 µM 7.23E-4 fatty acid desaturase (OLE1) 183 0.00358
17 SGTC_3315 9136395 2.64 68.5 µM 8.79E-4 80 0.00413
18 SGTC_2050 5263056 2.63 171.0 µM 9.34E-4 fatty acid desaturase (OLE1) 136 0.00432
19 SGTC_763 3453-1109 2.59 128.0 µM 0.00106 99 0.00473
20 SGTC_2496 diffractaic acid 2.54 79.8 µM 0.00129 126 0.00547

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.315 2.84E-78 YMR110C HFD1 hom Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
0.301 4.13E-71 YBR169C SSE2 hom Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication
0.297 3.25E-69 YNL105W_d RRT16_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription
0.295 1.97E-68 YPR024W YME1 hom Catalytic subunit of the mitochondrial inner membrane i-AAA protease complex, which is responsible for degradation of unfolded or misfolded mitochondrial gene products; also has a role in intermembrane space protein folding; mutation causes an elevated rate of mitochondrial turnover
0.285 7.74E-64 YBR003W COQ1 hom Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
0.282 2.09E-62 YPR146C_d YPR146C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.264 8.25E-55 YJL168C SET2 hom Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
0.255 4.02E-51 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.248 2.43E-48 YDR148C KGD2 hom Dihydrolipoyl transsuccinylase, component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated
0.243 2.43E-46 YDR204W COQ4 hom Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.233 1.61E-42 YML014W TRM9 hom tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
0.231 9.14E-42 YLR127C APC2 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p
0.229 3.00E-41 YNL111C CYB5 hom Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
0.227 1.29E-40 YLR239C LIP2 hom Lipoyl ligase, involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
0.227 2.30E-40 YLR021W IRC25 hom Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions