YLR200W / YKE2

Subunit of the heterohexameric Gim/prefoldin protein complex involved in the folding of alpha-tubulin, beta-tubulin, and actin

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR200W / YKE2

Click on Significant Values for Screen Details ID:SGTC_79|Compound:0052-0007|FD-Score:4.27|P-value:1.26E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:5.86|P-value:2.31E-7 ID:SGTC_146|Compound:3448-8650|FD-Score:6.11|P-value:7.32E-8 ID:SGTC_163|Compound:1327-0036|FD-Score:4.32|P-value:1.05E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:5.48|P-value:1.25E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:6.02|P-value:1.13E-7 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:5.41|P-value:1.66E-6 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:9.98|P-value:3.84E-18 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:13.30|P-value:1.22E-30 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:12.50|P-value:2.71E-27 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:11.70|P-value:3.26E-24 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:17.70|P-value:3.74E-53 ID:SGTC_313|Compound:3474-0040|FD-Score:4.90|P-value:1.27E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:-4.04|P-value:2.12E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:-4.03|P-value:2.20E-4 ID:SGTC_379|Compound:3970-0972|FD-Score:3.73|P-value:6.89E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:4.28|P-value:1.19E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.38|P-value:8.40E-5 ID:SGTC_648|Compound:0375-0473|FD-Score:5.42|P-value:1.56E-6 ID:SGTC_709|Compound:3807-1509|FD-Score:3.72|P-value:7.10E-4 ID:SGTC_717|Compound:1532-0246|FD-Score:-3.84|P-value:4.04E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:4.34|P-value:9.66E-5 ID:SGTC_748|Compound:0136-0228|FD-Score:4.73|P-value:2.43E-5 ID:SGTC_752|Compound:1896-0090|FD-Score:4.32|P-value:1.05E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.89|P-value:4.21E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.66|P-value:6.86E-4 ID:SGTC_897|Compound:1000-0139|FD-Score:8.31|P-value:4.06E-13 ID:SGTC_1128|Compound:3448-9350|FD-Score:4.13|P-value:1.95E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:5.49|P-value:1.19E-6 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:3.89|P-value:4.22E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:5.01|P-value:8.36E-6 ID:SGTC_1318|Compound:1280-3875|FD-Score:4.14|P-value:1.92E-4 ID:SGTC_1337|Compound:1486-1011|FD-Score:3.65|P-value:8.87E-4 ID:SGTC_1591|Compound:genipin|FD-Score:-3.63|P-value:7.61E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.98|P-value:3.20E-4 ID:SGTC_1616|Compound:st002734|FD-Score:4.47|P-value:6.11E-5 ID:SGTC_1643|Compound:st009866|FD-Score:11.50|P-value:1.51E-23 ID:SGTC_1653|Compound:st012112|FD-Score:3.62|P-value:9.70E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:6.12|P-value:6.95E-8 ID:SGTC_1681|Compound:tripelennamine|FD-Score:3.95|P-value:3.56E-4 ID:SGTC_1712|Compound:st032288|FD-Score:9.57|P-value:8.06E-17 ID:SGTC_1754|Compound:st042065|FD-Score:3.62|P-value:9.63E-4 ID:SGTC_1760|Compound:st045453|FD-Score:4.02|P-value:2.80E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.73|P-value:7.04E-4 ID:SGTC_1800|Compound:st050079|FD-Score:5.51|P-value:1.08E-6 ID:SGTC_1803|Compound:st050068|FD-Score:5.40|P-value:1.73E-6 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.88|P-value:1.38E-5 ID:SGTC_1875|Compound:nocodazole|FD-Score:5.46|P-value:1.32E-6 ID:SGTC_2007|Compound:4011539|FD-Score:3.66|P-value:8.56E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-3.89|P-value:3.40E-4 ID:SGTC_2051|Compound:5263410|FD-Score:4.48|P-value:6.03E-5 ID:SGTC_2106|Compound:5357045|FD-Score:3.72|P-value:7.09E-4 ID:SGTC_2108|Compound:5365811|FD-Score:4.01|P-value:2.96E-4 ID:SGTC_2146|Compound:5545642|FD-Score:3.90|P-value:4.10E-4 ID:SGTC_2344|Compound:7990960|FD-Score:3.73|P-value:6.98E-4 ID:SGTC_2372|Compound:9071633|FD-Score:9.40|P-value:2.60E-16 ID:SGTC_2389|Compound:9091542|FD-Score:-5.34|P-value:1.63E-6 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:5.44|P-value:1.46E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:10.30|P-value:3.68E-19 ID:SGTC_2651|Compound:1327-0036|FD-Score:4.78|P-value:2.02E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.85|P-value:4.84E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:8.90|P-value:8.60E-15 ID:SGTC_2817|Compound:7995342|FD-Score:5.75|P-value:3.82E-7 ID:SGTC_2912|Compound:7964298|FD-Score:5.06|P-value:6.86E-6 ID:SGTC_2922|Compound:7999257|FD-Score:5.98|P-value:1.34E-7 ID:SGTC_2925|Compound:9010536|FD-Score:3.87|P-value:4.55E-4 ID:SGTC_2935|Compound:9038016|FD-Score:7.45|P-value:7.05E-11 ID:SGTC_2946|Compound:9065502|FD-Score:-3.75|P-value:5.30E-4 ID:SGTC_2993|Compound:9054257|FD-Score:-4.75|P-value:1.75E-5 ID:SGTC_3027|Compound:9089541|FD-Score:-3.97|P-value:2.64E-4 ID:SGTC_3037|Compound:9091026|FD-Score:5.46|P-value:1.36E-6 ID:SGTC_3063|Compound:1327-0034|FD-Score:4.84|P-value:1.59E-5 ID:SGTC_3152|Compound:9099253|FD-Score:4.33|P-value:1.00E-4 ID:SGTC_3217|Compound:9129673|FD-Score:5.00|P-value:8.63E-6 ID:SGTC_3218|Compound:9131285|FD-Score:-4.57|P-value:3.38E-5 ID:SGTC_79|Compound:0052-0007|FD-Score:4.27|P-value:1.26E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:5.86|P-value:2.31E-7 ID:SGTC_146|Compound:3448-8650|FD-Score:6.11|P-value:7.32E-8 ID:SGTC_163|Compound:1327-0036|FD-Score:4.32|P-value:1.05E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:5.48|P-value:1.25E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:6.02|P-value:1.13E-7 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:5.41|P-value:1.66E-6 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:9.98|P-value:3.84E-18 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:13.30|P-value:1.22E-30 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:12.50|P-value:2.71E-27 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:11.70|P-value:3.26E-24 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:17.70|P-value:3.74E-53 ID:SGTC_313|Compound:3474-0040|FD-Score:4.90|P-value:1.27E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:-4.04|P-value:2.12E-4 ID:SGTC_367|Compound:0335-0881|FD-Score:-4.03|P-value:2.20E-4 ID:SGTC_379|Compound:3970-0972|FD-Score:3.73|P-value:6.89E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:4.28|P-value:1.19E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.38|P-value:8.40E-5 ID:SGTC_648|Compound:0375-0473|FD-Score:5.42|P-value:1.56E-6 ID:SGTC_709|Compound:3807-1509|FD-Score:3.72|P-value:7.10E-4 ID:SGTC_717|Compound:1532-0246|FD-Score:-3.84|P-value:4.04E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:4.34|P-value:9.66E-5 ID:SGTC_748|Compound:0136-0228|FD-Score:4.73|P-value:2.43E-5 ID:SGTC_752|Compound:1896-0090|FD-Score:4.32|P-value:1.05E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.89|P-value:4.21E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.66|P-value:6.86E-4 ID:SGTC_897|Compound:1000-0139|FD-Score:8.31|P-value:4.06E-13 ID:SGTC_1128|Compound:3448-9350|FD-Score:4.13|P-value:1.95E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:5.49|P-value:1.19E-6 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:3.89|P-value:4.22E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:5.01|P-value:8.36E-6 ID:SGTC_1318|Compound:1280-3875|FD-Score:4.14|P-value:1.92E-4 ID:SGTC_1337|Compound:1486-1011|FD-Score:3.65|P-value:8.87E-4 ID:SGTC_1591|Compound:genipin|FD-Score:-3.63|P-value:7.61E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.98|P-value:3.20E-4 ID:SGTC_1616|Compound:st002734|FD-Score:4.47|P-value:6.11E-5 ID:SGTC_1643|Compound:st009866|FD-Score:11.50|P-value:1.51E-23 ID:SGTC_1653|Compound:st012112|FD-Score:3.62|P-value:9.70E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:6.12|P-value:6.95E-8 ID:SGTC_1681|Compound:tripelennamine|FD-Score:3.95|P-value:3.56E-4 ID:SGTC_1712|Compound:st032288|FD-Score:9.57|P-value:8.06E-17 ID:SGTC_1754|Compound:st042065|FD-Score:3.62|P-value:9.63E-4 ID:SGTC_1760|Compound:st045453|FD-Score:4.02|P-value:2.80E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.73|P-value:7.04E-4 ID:SGTC_1800|Compound:st050079|FD-Score:5.51|P-value:1.08E-6 ID:SGTC_1803|Compound:st050068|FD-Score:5.40|P-value:1.73E-6 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.88|P-value:1.38E-5 ID:SGTC_1875|Compound:nocodazole|FD-Score:5.46|P-value:1.32E-6 ID:SGTC_2007|Compound:4011539|FD-Score:3.66|P-value:8.56E-4 ID:SGTC_2008|Compound:4012253|FD-Score:-3.89|P-value:3.40E-4 ID:SGTC_2051|Compound:5263410|FD-Score:4.48|P-value:6.03E-5 ID:SGTC_2106|Compound:5357045|FD-Score:3.72|P-value:7.09E-4 ID:SGTC_2108|Compound:5365811|FD-Score:4.01|P-value:2.96E-4 ID:SGTC_2146|Compound:5545642|FD-Score:3.90|P-value:4.10E-4 ID:SGTC_2344|Compound:7990960|FD-Score:3.73|P-value:6.98E-4 ID:SGTC_2372|Compound:9071633|FD-Score:9.40|P-value:2.60E-16 ID:SGTC_2389|Compound:9091542|FD-Score:-5.34|P-value:1.63E-6 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:5.44|P-value:1.46E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:10.30|P-value:3.68E-19 ID:SGTC_2651|Compound:1327-0036|FD-Score:4.78|P-value:2.02E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.85|P-value:4.84E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:8.90|P-value:8.60E-15 ID:SGTC_2817|Compound:7995342|FD-Score:5.75|P-value:3.82E-7 ID:SGTC_2912|Compound:7964298|FD-Score:5.06|P-value:6.86E-6 ID:SGTC_2922|Compound:7999257|FD-Score:5.98|P-value:1.34E-7 ID:SGTC_2925|Compound:9010536|FD-Score:3.87|P-value:4.55E-4 ID:SGTC_2935|Compound:9038016|FD-Score:7.45|P-value:7.05E-11 ID:SGTC_2946|Compound:9065502|FD-Score:-3.75|P-value:5.30E-4 ID:SGTC_2993|Compound:9054257|FD-Score:-4.75|P-value:1.75E-5 ID:SGTC_3027|Compound:9089541|FD-Score:-3.97|P-value:2.64E-4 ID:SGTC_3037|Compound:9091026|FD-Score:5.46|P-value:1.36E-6 ID:SGTC_3063|Compound:1327-0034|FD-Score:4.84|P-value:1.59E-5 ID:SGTC_3152|Compound:9099253|FD-Score:4.33|P-value:1.00E-4 ID:SGTC_3217|Compound:9129673|FD-Score:5.00|P-value:8.63E-6 ID:SGTC_3218|Compound:9131285|FD-Score:-4.57|P-value:3.38E-5

Top fitness defect scores for YLR200W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_243 5hr heat shock (37°C) + splitomicin 17.70 1.0 µM 3.74E-53 heat shock/prefoldin 4 1.48E-70
2 SGTC_240 5hr heat shock (37°C) + Streptovitacin 13.30 300.0 µM 1.22E-30 heat shock/prefoldin 5 1.87E-40
3 SGTC_241 5hr heat shock (37°C) + cantharidin 12.50 155.4 µM 2.71E-27 cell wall signaling 8 5.53E-36
4 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 11.70 4.6 µM 3.26E-24 heat shock/prefoldin 4 7.25E-32
5 SGTC_1643 st009866 11.50 20.2 µM 1.51E-23 7 5.64E-31
6 SGTC_2582 agaric acid 10.30 100.0 µM 3.68E-19 redox potentiating 59 4.09E-25
7 SGTC_239 5hr heat shock (37°C) + mitomycin C 9.98 819.4 µM 3.84E-18 heat shock/prefoldin 4 9.40E-24
8 SGTC_1712 st032288 9.57 53.4 µM 8.06E-17 calcium & mitochondrial duress 28 5.48E-22
9 SGTC_2372 9071633 9.40 200.0 µM 2.60E-16 1 2.63E-21
10 SGTC_2688 kanamycin b 8.90 96.7 µM 8.60E-15 calcium & mitochondrial duress 45 2.81E-19
11 SGTC_897 1000-0139 8.31 31.4 µM 4.06E-13 9 4.82E-17
12 SGTC_2935 9038016 7.45 30.0 µM 7.05E-11 3 4.71E-14
13 SGTC_1680 diphenhydramine 6.12 100.0 µM 6.95E-8 NEO1 5 4.63E-10
14 SGTC_146 3448-8650 6.11 53.1 µM 7.32E-8 NEO1-PIK1 52 4.96E-10
15 SGTC_221 trifluoperazine 6.02 10.3 µM 1.13E-7 NEO1-PIK1 121 8.82E-10
16 SGTC_2922 7999257 5.98 32.5 µM 1.34E-7 2 1.11E-9
17 SGTC_98 0074-0023 5.86 107.7 µM 2.31E-7 Golgi 12 2.29E-9
18 SGTC_2817 7995342 5.75 71.4 µM 3.82E-7 4 4.48E-9
19 SGTC_1800 st050079 5.51 29.1 µM 1.08E-6 NEO1-PIK1 55 1.79E-8
20 SGTC_1249 0481-0015 5.49 10.2 µM 1.19E-6 9 2.03E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.457 4.04E-173 YML094C-A_d YML094C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO
0.423 1.63E-145 YLR027C AAT2 hom Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.379 4.37E-115 YCR063W BUD31 hom Component of the SF3b subcomplex of the U2 snRNP; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
0.376 4.03E-113 YDR260C SWM1 hom Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
0.306 1.85E-73 YJL179W PFD1 hom Subunit of heterohexameric prefoldin, which binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin
0.290 5.67E-66 YGR078C PAC10 hom Part of the heteromeric co-chaperone GimC/prefoldin complex, which promotes efficient protein folding
0.280 1.30E-61 YDR457W TOM1 hom E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
0.273 2.36E-58 YDR069C DOA4 hom Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.268 2.32E-56 YCR064C_d YCR064C_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene BUD31
0.253 5.08E-50 YNL098C RAS2 hom GTP-binding protein that regulates the nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; homolog of mammalian Ras proto-oncogenes
0.241 1.60E-45 YHR041C SRB2 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance
0.226 3.53E-40 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.219 1.21E-37 YOR001W RRP6 hom Nuclear exosome exonuclease component; has 3'-5' exonuclease activity; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
0.211 3.86E-35 YNL246W VPS75 hom NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress
0.211 5.88E-35 YCR077C PAT1 hom Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress