YLR207W / HRD3

Resident protein of the ER membrane that plays a central role in ER-associated protein degradation (ERAD), forms HRD complex with Hrd1p and ERAD determinants that engages in lumen to cytosol communication and coordination of ERAD events

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR207W / HRD3

Click on Significant Values for Screen Details ID:SGTC_7|Compound:0192-1582|FD-Score:3.86|P-value:1.11E-5 ID:SGTC_55|Compound:0767-0285|FD-Score:2.85|P-value:8.66E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.49|P-value:3.97E-7 ID:SGTC_67|Compound:0335-0847|FD-Score:3.26|P-value:1.69E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:5.92|P-value:3.61E-11 ID:SGTC_87|Compound:0100-0052|FD-Score:3.75|P-value:1.85E-5 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:3.12|P-value:2.96E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.09|P-value:3.32E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.15|P-value:2.50E-6 ID:SGTC_341|Compound:1486-1430|FD-Score:-3.02|P-value:4.55E-4 ID:SGTC_348|Compound:k064-0012|FD-Score:-2.94|P-value:6.18E-4 ID:SGTC_427|Compound:1354-0053|FD-Score:3.26|P-value:1.64E-4 ID:SGTC_435|Compound:c-PAF|FD-Score:2.81|P-value:9.99E-4 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:3.22|P-value:1.99E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:4.75|P-value:8.88E-8 ID:SGTC_622|Compound:1348-1566|FD-Score:2.83|P-value:9.14E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:3.14|P-value:2.74E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:-2.88|P-value:7.78E-4 ID:SGTC_818|Compound:4072-2707|FD-Score:-3.01|P-value:4.55E-4 ID:SGTC_821|Compound:1114-0266|FD-Score:3.11|P-value:3.14E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:3.02|P-value:4.55E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:-2.86|P-value:8.35E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:3.08|P-value:3.48E-4 ID:SGTC_1185|Compound:1319-0113|FD-Score:-3.27|P-value:1.63E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:3.07|P-value:3.72E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:2.90|P-value:7.10E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:-2.88|P-value:7.71E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:-4.15|P-value:2.51E-6 ID:SGTC_1445|Compound:4239-0156|FD-Score:2.81|P-value:9.99E-4 ID:SGTC_1570|Compound:verbenone|FD-Score:2.97|P-value:5.35E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:3.17|P-value:2.40E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:2.95|P-value:5.77E-4 ID:SGTC_1591|Compound:genipin|FD-Score:-3.16|P-value:2.53E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.73|P-value:2.04E-5 ID:SGTC_1604|Compound:st002052|FD-Score:3.22|P-value:1.98E-4 ID:SGTC_1621|Compound:st003705|FD-Score:4.44|P-value:5.24E-7 ID:SGTC_1650|Compound:st011916|FD-Score:5.23|P-value:4.27E-9 ID:SGTC_1655|Compound:st012546|FD-Score:2.95|P-value:5.77E-4 ID:SGTC_1670|Compound:st016595|FD-Score:-3.96|P-value:6.59E-6 ID:SGTC_1733|Compound:st037357|FD-Score:3.26|P-value:1.68E-4 ID:SGTC_1740|Compound:st038286|FD-Score:2.90|P-value:7.22E-4 ID:SGTC_1744|Compound:st037937|FD-Score:2.95|P-value:5.98E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.08|P-value:3.50E-4 ID:SGTC_1778|Compound:st046819|FD-Score:3.48|P-value:6.58E-5 ID:SGTC_1961|Compound:st076550|FD-Score:3.17|P-value:2.49E-4 ID:SGTC_2105|Compound:5350118|FD-Score:-3.12|P-value:3.05E-4 ID:SGTC_2112|Compound:5488546|FD-Score:-2.86|P-value:8.41E-4 ID:SGTC_2122|Compound:5258151|FD-Score:-3.10|P-value:3.23E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.93|P-value:2.97E-8 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.33|P-value:1.25E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:-3.48|P-value:6.56E-5 ID:SGTC_2605|Compound:digitonin|FD-Score:3.32|P-value:1.28E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:-4.66|P-value:1.46E-7 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:3.03|P-value:4.36E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:2.94|P-value:6.08E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.03|P-value:4.23E-4 ID:SGTC_2953|Compound:9074162|FD-Score:2.90|P-value:7.19E-4 ID:SGTC_3125|Compound:9127534|FD-Score:3.40|P-value:9.26E-5 ID:SGTC_3203|Compound:9113543|FD-Score:3.22|P-value:1.96E-4 ID:SGTC_3208|Compound:9114115|FD-Score:-4.25|P-value:1.48E-6 ID:SGTC_3326|Compound:9140064|FD-Score:-3.15|P-value:2.64E-4 ID:SGTC_3328|Compound:9140624|FD-Score:-2.83|P-value:9.18E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:3.86|P-value:1.11E-5 ID:SGTC_55|Compound:0767-0285|FD-Score:2.85|P-value:8.66E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:4.49|P-value:3.97E-7 ID:SGTC_67|Compound:0335-0847|FD-Score:3.26|P-value:1.69E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:5.92|P-value:3.61E-11 ID:SGTC_87|Compound:0100-0052|FD-Score:3.75|P-value:1.85E-5 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:3.12|P-value:2.96E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.09|P-value:3.32E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.15|P-value:2.50E-6 ID:SGTC_341|Compound:1486-1430|FD-Score:-3.02|P-value:4.55E-4 ID:SGTC_348|Compound:k064-0012|FD-Score:-2.94|P-value:6.18E-4 ID:SGTC_427|Compound:1354-0053|FD-Score:3.26|P-value:1.64E-4 ID:SGTC_435|Compound:c-PAF|FD-Score:2.81|P-value:9.99E-4 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:3.22|P-value:1.99E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:4.75|P-value:8.88E-8 ID:SGTC_622|Compound:1348-1566|FD-Score:2.83|P-value:9.14E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:3.14|P-value:2.74E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:-2.88|P-value:7.78E-4 ID:SGTC_818|Compound:4072-2707|FD-Score:-3.01|P-value:4.55E-4 ID:SGTC_821|Compound:1114-0266|FD-Score:3.11|P-value:3.14E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:3.02|P-value:4.55E-4 ID:SGTC_944|Compound:3370-0224|FD-Score:-2.86|P-value:8.35E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:3.08|P-value:3.48E-4 ID:SGTC_1185|Compound:1319-0113|FD-Score:-3.27|P-value:1.63E-4 ID:SGTC_1234|Compound:0326-0494|FD-Score:3.07|P-value:3.72E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:2.90|P-value:7.10E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:-2.88|P-value:7.71E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:-4.15|P-value:2.51E-6 ID:SGTC_1445|Compound:4239-0156|FD-Score:2.81|P-value:9.99E-4 ID:SGTC_1570|Compound:verbenone|FD-Score:2.97|P-value:5.35E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:3.17|P-value:2.40E-4 ID:SGTC_1584|Compound:6-fluoro-dl-tryptophan|FD-Score:2.95|P-value:5.77E-4 ID:SGTC_1591|Compound:genipin|FD-Score:-3.16|P-value:2.53E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.73|P-value:2.04E-5 ID:SGTC_1604|Compound:st002052|FD-Score:3.22|P-value:1.98E-4 ID:SGTC_1621|Compound:st003705|FD-Score:4.44|P-value:5.24E-7 ID:SGTC_1650|Compound:st011916|FD-Score:5.23|P-value:4.27E-9 ID:SGTC_1655|Compound:st012546|FD-Score:2.95|P-value:5.77E-4 ID:SGTC_1670|Compound:st016595|FD-Score:-3.96|P-value:6.59E-6 ID:SGTC_1733|Compound:st037357|FD-Score:3.26|P-value:1.68E-4 ID:SGTC_1740|Compound:st038286|FD-Score:2.90|P-value:7.22E-4 ID:SGTC_1744|Compound:st037937|FD-Score:2.95|P-value:5.98E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.08|P-value:3.50E-4 ID:SGTC_1778|Compound:st046819|FD-Score:3.48|P-value:6.58E-5 ID:SGTC_1961|Compound:st076550|FD-Score:3.17|P-value:2.49E-4 ID:SGTC_2105|Compound:5350118|FD-Score:-3.12|P-value:3.05E-4 ID:SGTC_2112|Compound:5488546|FD-Score:-2.86|P-value:8.41E-4 ID:SGTC_2122|Compound:5258151|FD-Score:-3.10|P-value:3.23E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.93|P-value:2.97E-8 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.33|P-value:1.25E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:-3.48|P-value:6.56E-5 ID:SGTC_2605|Compound:digitonin|FD-Score:3.32|P-value:1.28E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:-4.66|P-value:1.46E-7 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:3.03|P-value:4.36E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:2.94|P-value:6.08E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:-3.03|P-value:4.23E-4 ID:SGTC_2953|Compound:9074162|FD-Score:2.90|P-value:7.19E-4 ID:SGTC_3125|Compound:9127534|FD-Score:3.40|P-value:9.26E-5 ID:SGTC_3203|Compound:9113543|FD-Score:3.22|P-value:1.96E-4 ID:SGTC_3208|Compound:9114115|FD-Score:-4.25|P-value:1.48E-6 ID:SGTC_3326|Compound:9140064|FD-Score:-3.15|P-value:2.64E-4 ID:SGTC_3328|Compound:9140624|FD-Score:-2.83|P-value:9.18E-4

Top fitness defect scores for YLR207W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_86 0417-1630 5.92 471.9 µM 3.61E-11 ubiquinone biosynthesis & proteasome 89 1.58E-9
2 SGTC_1650 st011916 5.23 45.8 µM 4.27E-9 3 8.31E-8
3 SGTC_2407 paclitaxel 4.93 400.0 µM 2.97E-8 16 4.17E-7
4 SGTC_513 4245-1575 4.75 41.7 µM 8.88E-8 unfolded protein response 82 1.04E-6
5 SGTC_59 0330-0160 4.49 1.3 µM 3.97E-7 85 3.60E-6
6 SGTC_1621 st003705 4.44 32.4 µM 5.24E-7 calcium & mitochondrial duress 15 4.54E-6
7 SGTC_7 0192-1582 3.86 4.2 µM 1.11E-5 61 5.75E-5
8 SGTC_87 0100-0052 3.75 35.5 µM 1.85E-5 123 8.85E-5
9 SGTC_1778 st046819 3.48 58.9 µM 6.58E-5 42 2.55E-4
10 SGTC_3125 9127534 3.40 49.5 µM 9.26E-5 24 3.39E-4
11 SGTC_2582 agaric acid 3.33 100.0 µM 1.25E-4 redox potentiating 386 4.35E-4
12 SGTC_2605 digitonin 3.32 300.0 nM 1.28E-4 56 4.43E-4
13 SGTC_427 1354-0053 3.26 47.3 µM 1.64E-4 49 5.47E-4
14 SGTC_1733 st037357 3.26 54.9 µM 1.68E-4 60S ribosome export 74 5.59E-4
15 SGTC_67 0335-0847 3.26 266.0 µM 1.69E-4 ubiquinone biosynthesis & proteasome 159 5.60E-4
16 SGTC_3203 9113543 3.22 49.5 µM 1.96E-4 63 6.34E-4
17 SGTC_1604 st002052 3.22 72.4 µM 1.98E-4 TSC3-RPN4 47 6.39E-4
18 SGTC_506 n9-isopropylolomoucine 3.22 153.0 µM 1.99E-4 32 6.42E-4
19 SGTC_1581 himbacine 3.17 57.9 µM 2.40E-4 Golgi 71 7.51E-4
20 SGTC_1961 st076550 3.17 68.7 µM 2.49E-4 36 7.75E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.183 1.50E-26 YLR202C_d YLR202C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron
0.178 2.25E-25 YOL013C HRD1 hom Ubiquitin-protein ligase; required for endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon
0.153 5.32E-19 YBR082C UBC4 hom Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication
0.148 5.84E-18 YER087C-A_d YER087C-A_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; overlaps the uncharacterized gene YER087W, a putative tRNA synthetase
0.147 1.34E-17 YLR292C SEC72 hom Non-essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.143 9.00E-17 YJL155C FBP26 hom Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress
0.143 9.33E-17 YNR041C COQ2 hom Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis
0.141 1.80E-16 YDL002C NHP10 hom Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair
0.139 6.27E-16 YCL014W BUD3 hom Protein involved in bud-site selection and required for axial budding pattern; localizes with septins to bud neck in mitosis and may constitute an axial landmark for next round of budding
0.137 1.33E-15 YIL090W ICE2 hom Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells
0.137 1.40E-15 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.136 2.59E-15 YBR003W COQ1 hom Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
0.133 1.06E-14 YNL215W IES2 hom Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
0.133 1.33E-14 YGR208W SER2 hom Phosphoserine phosphatase of the phosphoglycerate pathway, involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
0.132 1.77E-14 YMR110C HFD1 hom Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder