YLR212C / TUB4

Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR212C / TUB4

Click on Significant Values for Screen Details ID:SGTC_163|Compound:1327-0036|FD-Score:2.90|P-value:4.52E-5 ID:SGTC_222|Compound:nsc-310342|FD-Score:2.47|P-value:3.99E-4 ID:SGTC_223|Compound:busulfan|FD-Score:4.26|P-value:5.91E-9 ID:SGTC_224|Compound:benfluorex|FD-Score:2.42|P-value:5.17E-4 ID:SGTC_225|Compound:nsc-122657|FD-Score:3.64|P-value:5.13E-7 ID:SGTC_367|Compound:0335-0881|FD-Score:3.44|P-value:1.90E-6 ID:SGTC_372|Compound:0155-0182|FD-Score:-2.59|P-value:4.89E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.12|P-value:1.72E-8 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:-3.47|P-value:4.15E-6 ID:SGTC_487|Compound:nimodipine|FD-Score:2.53|P-value:3.03E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:3.62|P-value:5.92E-7 ID:SGTC_610|Compound:4215-0184|FD-Score:2.38|P-value:6.19E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:4.98|P-value:1.52E-11 ID:SGTC_612|Compound:0302-0167|FD-Score:5.84|P-value:3.51E-15 ID:SGTC_615|Compound:k087-0205|FD-Score:4.25|P-value:6.38E-9 ID:SGTC_616|Compound:4092-0450|FD-Score:3.70|P-value:3.43E-7 ID:SGTC_730|Compound:1436-0052|FD-Score:3.44|P-value:1.84E-6 ID:SGTC_737|Compound:1950-0117|FD-Score:2.45|P-value:4.39E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:8.73|P-value:2.74E-31 ID:SGTC_797|Compound:k781-0471|FD-Score:2.31|P-value:8.48E-4 ID:SGTC_849|Compound:0386-0493|FD-Score:-3.23|P-value:1.76E-5 ID:SGTC_916|Compound:4466-0038|FD-Score:11.00|P-value:1.73E-48 ID:SGTC_987|Compound:1497-1013|FD-Score:2.77|P-value:8.92E-5 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:2.72|P-value:1.15E-4 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:2.75|P-value:9.99E-5 ID:SGTC_1169|Compound:aniline yellow|FD-Score:2.89|P-value:4.69E-5 ID:SGTC_1182|Compound:1222-0449|FD-Score:-2.83|P-value:1.47E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:2.51|P-value:3.29E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:2.41|P-value:5.37E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:3.62|P-value:5.81E-7 ID:SGTC_1696|Compound:st025591|FD-Score:-2.76|P-value:2.08E-4 ID:SGTC_1699|Compound:st031318|FD-Score:-3.34|P-value:8.97E-6 ID:SGTC_1710|Compound:st031758|FD-Score:2.31|P-value:8.33E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.41|P-value:2.21E-6 ID:SGTC_1880|Compound:MG-132|FD-Score:5.34|P-value:5.38E-13 ID:SGTC_1885|Compound:st057546|FD-Score:2.39|P-value:5.89E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-4.40|P-value:6.52E-9 ID:SGTC_2113|Compound:5491891|FD-Score:-2.94|P-value:8.51E-5 ID:SGTC_2148|Compound:5546263|FD-Score:2.71|P-value:1.23E-4 ID:SGTC_2246|Compound:7293507|FD-Score:2.38|P-value:5.98E-4 ID:SGTC_2324|Compound:6149169|FD-Score:2.37|P-value:6.54E-4 ID:SGTC_2450|Compound:5739737|FD-Score:-2.58|P-value:5.04E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:6.57|P-value:1.19E-18 ID:SGTC_2602|Compound:kawain|FD-Score:2.36|P-value:6.85E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:5.69|P-value:1.65E-14 ID:SGTC_2651|Compound:1327-0036|FD-Score:2.57|P-value:2.48E-4 ID:SGTC_3004|Compound:9076122|FD-Score:2.59|P-value:2.20E-4 ID:SGTC_3086|Compound:9117350|FD-Score:2.68|P-value:1.44E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-2.45|P-value:9.34E-4 ID:SGTC_3301|Compound:9125354|FD-Score:-3.37|P-value:7.41E-6 ID:SGTC_3304|Compound:9125678|FD-Score:-3.17|P-value:2.48E-5 ID:SGTC_3305|Compound:9127027|FD-Score:3.50|P-value:1.29E-6 ID:SGTC_3311|Compound:9131146|FD-Score:4.27|P-value:5.39E-9 ID:SGTC_163|Compound:1327-0036|FD-Score:2.90|P-value:4.52E-5 ID:SGTC_222|Compound:nsc-310342|FD-Score:2.47|P-value:3.99E-4 ID:SGTC_223|Compound:busulfan|FD-Score:4.26|P-value:5.91E-9 ID:SGTC_224|Compound:benfluorex|FD-Score:2.42|P-value:5.17E-4 ID:SGTC_225|Compound:nsc-122657|FD-Score:3.64|P-value:5.13E-7 ID:SGTC_367|Compound:0335-0881|FD-Score:3.44|P-value:1.90E-6 ID:SGTC_372|Compound:0155-0182|FD-Score:-2.59|P-value:4.89E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.12|P-value:1.72E-8 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:-3.47|P-value:4.15E-6 ID:SGTC_487|Compound:nimodipine|FD-Score:2.53|P-value:3.03E-4 ID:SGTC_609|Compound:0986-0026|FD-Score:3.62|P-value:5.92E-7 ID:SGTC_610|Compound:4215-0184|FD-Score:2.38|P-value:6.19E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:4.98|P-value:1.52E-11 ID:SGTC_612|Compound:0302-0167|FD-Score:5.84|P-value:3.51E-15 ID:SGTC_615|Compound:k087-0205|FD-Score:4.25|P-value:6.38E-9 ID:SGTC_616|Compound:4092-0450|FD-Score:3.70|P-value:3.43E-7 ID:SGTC_730|Compound:1436-0052|FD-Score:3.44|P-value:1.84E-6 ID:SGTC_737|Compound:1950-0117|FD-Score:2.45|P-value:4.39E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:8.73|P-value:2.74E-31 ID:SGTC_797|Compound:k781-0471|FD-Score:2.31|P-value:8.48E-4 ID:SGTC_849|Compound:0386-0493|FD-Score:-3.23|P-value:1.76E-5 ID:SGTC_916|Compound:4466-0038|FD-Score:11.00|P-value:1.73E-48 ID:SGTC_987|Compound:1497-1013|FD-Score:2.77|P-value:8.92E-5 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:2.72|P-value:1.15E-4 ID:SGTC_1116|Compound:jasmonic acid|FD-Score:2.75|P-value:9.99E-5 ID:SGTC_1169|Compound:aniline yellow|FD-Score:2.89|P-value:4.69E-5 ID:SGTC_1182|Compound:1222-0449|FD-Score:-2.83|P-value:1.47E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:2.51|P-value:3.29E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:2.41|P-value:5.37E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:3.62|P-value:5.81E-7 ID:SGTC_1696|Compound:st025591|FD-Score:-2.76|P-value:2.08E-4 ID:SGTC_1699|Compound:st031318|FD-Score:-3.34|P-value:8.97E-6 ID:SGTC_1710|Compound:st031758|FD-Score:2.31|P-value:8.33E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.41|P-value:2.21E-6 ID:SGTC_1880|Compound:MG-132|FD-Score:5.34|P-value:5.38E-13 ID:SGTC_1885|Compound:st057546|FD-Score:2.39|P-value:5.89E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-4.40|P-value:6.52E-9 ID:SGTC_2113|Compound:5491891|FD-Score:-2.94|P-value:8.51E-5 ID:SGTC_2148|Compound:5546263|FD-Score:2.71|P-value:1.23E-4 ID:SGTC_2246|Compound:7293507|FD-Score:2.38|P-value:5.98E-4 ID:SGTC_2324|Compound:6149169|FD-Score:2.37|P-value:6.54E-4 ID:SGTC_2450|Compound:5739737|FD-Score:-2.58|P-value:5.04E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:6.57|P-value:1.19E-18 ID:SGTC_2602|Compound:kawain|FD-Score:2.36|P-value:6.85E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:5.69|P-value:1.65E-14 ID:SGTC_2651|Compound:1327-0036|FD-Score:2.57|P-value:2.48E-4 ID:SGTC_3004|Compound:9076122|FD-Score:2.59|P-value:2.20E-4 ID:SGTC_3086|Compound:9117350|FD-Score:2.68|P-value:1.44E-4 ID:SGTC_3212|Compound:9128307|FD-Score:-2.45|P-value:9.34E-4 ID:SGTC_3301|Compound:9125354|FD-Score:-3.37|P-value:7.41E-6 ID:SGTC_3304|Compound:9125678|FD-Score:-3.17|P-value:2.48E-5 ID:SGTC_3305|Compound:9127027|FD-Score:3.50|P-value:1.29E-6 ID:SGTC_3311|Compound:9131146|FD-Score:4.27|P-value:5.39E-9

Top fitness defect scores for YLR212C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_916 4466-0038 11.00 42.0 nM 1.73E-48 cell wall 6 1.61E-28 0.21
2 SGTC_773 4358-1457 8.73 44.6 µM 2.74E-31 3 1.33E-18 0.67
3 SGTC_2584 androsterone 6.57 48.7 µM 1.19E-18 cell wall 10 2.57E-11 0.68
4 SGTC_612 0302-0167 5.84 22.4 µM 3.51E-15 copper-dependent oxidative stress 2 2.58E-9 0.69
5 SGTC_2619 obliquin 5.69 87.9 µM 1.65E-14 cell wall 16 6.32E-9 0.12
6 SGTC_1880 MG-132 5.34 525.0 µM 5.38E-13 15 4.73E-8 0.37
7 SGTC_611 3453-1652 4.98 23.7 µM 1.52E-11 12 3.26E-7 0.04
8 SGTC_3311 9131146 4.27 2.9 µM 5.39E-9 cell wall 18 9.77E-6 0.28
9 SGTC_223 busulfan 4.26 5.0 mM 5.91E-9 1 1.03E-5 0.34
10 SGTC_615 k087-0205 4.25 167.0 µM 6.38E-9 2 1.08E-5 0.56
11 SGTC_467 ro 31-8220 4.12 72.8 µM 1.72E-8 calcium & mitochondrial duress 16 1.92E-5 0.02
12 SGTC_616 4092-0450 3.70 108.0 µM 3.43E-7 4 1.09E-4 0.19
13 SGTC_225 nsc-122657 3.64 1.0 µM 5.13E-7 4 1.38E-4 0.02
14 SGTC_1581 himbacine 3.62 57.9 µM 5.81E-7 Golgi 6 1.48E-4 0.01
15 SGTC_609 0986-0026 3.62 86.3 µM 5.92E-7 calcium & mitochondrial duress 16 1.49E-4 0.14
16 SGTC_3305 9127027 3.50 53.8 µM 1.29E-6 8 2.35E-4 0.00
17 SGTC_730 1436-0052 3.44 41.2 µM 1.84E-6 mitochondrial processes 19 2.89E-4 0.08
18 SGTC_367 0335-0881 3.44 7.1 µM 1.90E-6 ubiquinone biosynthesis & proteasome 29 2.95E-4 7.23E-4
19 SGTC_1783 st039459 3.41 87.5 µM 2.21E-6 Golgi 13 3.22E-4 0.03
20 SGTC_163 1327-0036 2.90 7.5 µM 4.52E-5 72 0.00187 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.362 5.04E-104 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.315 3.90E-78 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.259 3.19E-52 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.243 2.19E-46 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.234 1.55E-42 YLR271W_p YLR271W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
0.229 4.16E-41 YNL126W SPC98 het Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque
0.225 9.79E-40 YDR053W_d YDR053W_d het Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex
0.225 1.16E-39 YBR196C PGI1 het Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
0.222 1.16E-38 YBL061C SKT5 hom Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
0.216 1.34E-36 YMR001C CDC5 het Polo-like kinase with multiple functions in mitosis and cytokinesis through substrate phosphorylation, also functions in adaptation to DNA damage during meiosis; has similarity to Xenopus Plx1 and S. pombe Plo1p; possible Cdc28p substrate
0.216 1.33E-36 YDR318W MCM21 hom Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2
0.212 1.89E-35 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.211 7.67E-35 YML117W NAB6 hom Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
0.204 8.08E-33 YEL061C CIN8 hom Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation
0.203 1.44E-32 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair