HIPHOP chemogenomics database

YLR218C / COA4

Twin Cx(9)C protein involved in cytochrome c oxidase assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors

Zygosity: Homozygous strain

fixedexpanded
Profile for YLR218C / COA4

Click on Significant Values for Screen Details

Top fitness defect scores for YLR218C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_236	1509-0011	12.60	5.3 µM	5.81E-37		137	7.79E-37
2	SGTC_1635	st007035	9.23	42.4 µM	1.01E-20		85	1.37E-20
3	SGTC_386	1509-0011	6.91	3.1 µM	1.79E-12		78	2.34E-12
4	SGTC_2035	5154463	5.06	94.6 µM	1.69E-7		3	2.11E-7
5	SGTC_2329	6138490	4.46	200.0 µM	3.35E-6		17	4.12E-6
6	SGTC_3214	9129694	4.43	49.5 µM	3.87E-6		16	4.75E-6
7	SGTC_2530	3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene	4.42	92.6 µM	3.97E-6		17	4.88E-6
8	SGTC_464	bw-b 70c	4.38	158.0 µM	4.88E-6		13	5.99E-6
9	SGTC_1151	3909-8316	4.31	15.3 µM	6.53E-6		11	7.99E-6
10	SGTC_1158	4092-0494	4.19	91.8 µM	1.15E-5		12	1.41E-5
11	SGTC_842	0271-0008	4.04	11.1 µM	2.17E-5	heme biosynthesis & mitochondrial translocase	17	2.64E-5
12	SGTC_2525	harmine	3.88	47.9 µM	4.28E-5	ergosterol biosynthesis	11	5.17E-5
13	SGTC_1023	4482-3888	3.85	16.0 µM	4.86E-5	TSC3-RPN4	20	5.86E-5
14	SGTC_1167	k781-0610	3.80	148.0 µM	5.94E-5	RNA processing & uracil transport	31	7.16E-5
15	SGTC_402	epigallocatechin gallate	3.80	10.0 µM	6.03E-5		32	7.26E-5
16	SGTC_2635	hydroquinidine	3.79	100.0 µM	6.18E-5		27	7.43E-5
17	SGTC_2030	5175110	3.66	200.0 µM	1.06E-4	Golgi	33	1.27E-4
18	SGTC_1163	k060-0048	3.64	49.9 µM	1.13E-4		32	1.35E-4
19	SGTC_310	1273-0058	3.64	1.1 µM	1.14E-4	mitochondrial stress	267	1.36E-4
20	SGTC_1577	3(2'-chlorophenyl)-7-ethoxycoumarin	3.53	66.5 µM	1.74E-4		24	2.08E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.513	2.26E-224	YJL166W	QCR8	hom	Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.509	1.29E-220	YDR230W_d	YDR230W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.465	4.67E-180	YMR151W_d	YIM2_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.462	9.93E-177	YPL013C	MRPS16	hom	Mitochondrial ribosomal protein of the small subunit
0.461	4.06E-176	YPL215W	CBP3	hom	Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.454	1.27E-170	YEL059C-A	SOM1	hom	Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.451	4.29E-168	YJL022W_d	YJL022W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130
0.451	1.04E-167	YHR116W	COX23	hom	Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
0.450	8.77E-167	YDR375C	BCS1	hom	Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.446	8.00E-164	YJR120W	YJR120W	hom	Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.445	1.85E-162	YOR065W	CYT1	hom	Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
0.444	3.12E-162	YGR183C	QCR9	hom	Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
0.444	1.03E-161	YPL132W	COX11	hom	Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
0.436	1.93E-155	YMR035W	IMP2	hom	Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.431	5.43E-152	YOR330C	MIP1	hom	Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit