YLR219W / MSC3

Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR219W / MSC3

Click on Significant Values for Screen Details ID:SGTC_19|Compound:0279-0214|FD-Score:2.75|P-value:9.48E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:-3.31|P-value:1.43E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:3.07|P-value:2.66E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:3.22|P-value:1.46E-4 ID:SGTC_133|Compound:0724-0033|FD-Score:3.34|P-value:8.65E-5 ID:SGTC_136|Compound:0097-0015|FD-Score:-3.53|P-value:5.29E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:3.47|P-value:4.82E-5 ID:SGTC_369|Compound:0957-0393|FD-Score:4.56|P-value:1.55E-7 ID:SGTC_372|Compound:0155-0182|FD-Score:3.57|P-value:2.90E-5 ID:SGTC_481|Compound:bay k-8644|FD-Score:-2.94|P-value:6.46E-4 ID:SGTC_494|Compound:Boc-Gly-Val-Val-CHO|FD-Score:2.98|P-value:3.83E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:3.02|P-value:3.36E-4 ID:SGTC_500|Compound:h-89|FD-Score:4.20|P-value:1.22E-6 ID:SGTC_545|Compound:4218-0007|FD-Score:-4.40|P-value:6.43E-7 ID:SGTC_551|Compound:k048-0028|FD-Score:5.37|P-value:9.13E-10 ID:SGTC_557|Compound:1315-0110|FD-Score:3.03|P-value:3.13E-4 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:3.16|P-value:1.85E-4 ID:SGTC_693|Compound:4161-2696|FD-Score:3.54|P-value:3.34E-5 ID:SGTC_839|Compound:0122-0028|FD-Score:3.80|P-value:9.56E-6 ID:SGTC_859|Compound:0439-0102|FD-Score:2.87|P-value:5.92E-4 ID:SGTC_869|Compound:0564-0080|FD-Score:4.82|P-value:3.24E-8 ID:SGTC_872|Compound:0588-0139|FD-Score:-3.37|P-value:1.06E-4 ID:SGTC_903|Compound:1134-0042|FD-Score:4.39|P-value:4.28E-7 ID:SGTC_955|Compound:1216-0110|FD-Score:-3.16|P-value:2.65E-4 ID:SGTC_1073|Compound:triclabendazole|FD-Score:3.12|P-value:2.20E-4 ID:SGTC_1086|Compound:cerivastatin|FD-Score:2.89|P-value:5.58E-4 ID:SGTC_1206|Compound:3978-0017|FD-Score:3.89|P-value:6.11E-6 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:-2.90|P-value:7.41E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.53|P-value:5.30E-5 ID:SGTC_1662|Compound:st014054|FD-Score:-2.90|P-value:7.41E-4 ID:SGTC_1699|Compound:st031318|FD-Score:-2.94|P-value:6.28E-4 ID:SGTC_1726|Compound:st035556|FD-Score:-4.13|P-value:2.78E-6 ID:SGTC_1739|Compound:st037196|FD-Score:3.95|P-value:4.53E-6 ID:SGTC_1742|Compound:st038235|FD-Score:-3.45|P-value:7.46E-5 ID:SGTC_1757|Compound:st041620|FD-Score:-3.11|P-value:3.28E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:3.01|P-value:3.43E-4 ID:SGTC_1879|Compound:gemcitabine|FD-Score:-3.73|P-value:2.03E-5 ID:SGTC_1884|Compound:5633444|FD-Score:2.88|P-value:5.80E-4 ID:SGTC_2048|Compound:5246248|FD-Score:3.42|P-value:5.98E-5 ID:SGTC_2051|Compound:5263410|FD-Score:-2.88|P-value:7.97E-4 ID:SGTC_2065|Compound:5221596|FD-Score:2.87|P-value:6.09E-4 ID:SGTC_2213|Compound:7173844|FD-Score:-3.04|P-value:4.26E-4 ID:SGTC_2351|Compound:9026858|FD-Score:4.68|P-value:7.66E-8 ID:SGTC_2405|Compound:ticlatone|FD-Score:3.25|P-value:1.27E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:4.08|P-value:2.34E-6 ID:SGTC_2436|Compound:5633522|FD-Score:2.84|P-value:6.87E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:-3.05|P-value:4.12E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-3.68|P-value:2.62E-5 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:3.18|P-value:1.69E-4 ID:SGTC_2591|Compound:skatole|FD-Score:4.69|P-value:7.35E-8 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:4.27|P-value:8.28E-7 ID:SGTC_2695|Compound:st077686|FD-Score:-3.47|P-value:6.79E-5 ID:SGTC_2709|Compound:st078560|FD-Score:3.93|P-value:4.92E-6 ID:SGTC_2710|Compound:st078562|FD-Score:-2.88|P-value:8.01E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.53|P-value:5.31E-5 ID:SGTC_3249|Compound:9135183|FD-Score:3.03|P-value:3.18E-4 ID:SGTC_3314|Compound:9135860|FD-Score:3.20|P-value:1.60E-4 ID:SGTC_3316|Compound:9136639|FD-Score:4.59|P-value:1.30E-7 ID:SGTC_19|Compound:0279-0214|FD-Score:2.75|P-value:9.48E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:-3.31|P-value:1.43E-4 ID:SGTC_127|Compound:0180-0333|FD-Score:3.07|P-value:2.66E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:3.22|P-value:1.46E-4 ID:SGTC_133|Compound:0724-0033|FD-Score:3.34|P-value:8.65E-5 ID:SGTC_136|Compound:0097-0015|FD-Score:-3.53|P-value:5.29E-5 ID:SGTC_362|Compound:0310-0100|FD-Score:3.47|P-value:4.82E-5 ID:SGTC_369|Compound:0957-0393|FD-Score:4.56|P-value:1.55E-7 ID:SGTC_372|Compound:0155-0182|FD-Score:3.57|P-value:2.90E-5 ID:SGTC_481|Compound:bay k-8644|FD-Score:-2.94|P-value:6.46E-4 ID:SGTC_494|Compound:Boc-Gly-Val-Val-CHO|FD-Score:2.98|P-value:3.83E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:3.02|P-value:3.36E-4 ID:SGTC_500|Compound:h-89|FD-Score:4.20|P-value:1.22E-6 ID:SGTC_545|Compound:4218-0007|FD-Score:-4.40|P-value:6.43E-7 ID:SGTC_551|Compound:k048-0028|FD-Score:5.37|P-value:9.13E-10 ID:SGTC_557|Compound:1315-0110|FD-Score:3.03|P-value:3.13E-4 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:3.16|P-value:1.85E-4 ID:SGTC_693|Compound:4161-2696|FD-Score:3.54|P-value:3.34E-5 ID:SGTC_839|Compound:0122-0028|FD-Score:3.80|P-value:9.56E-6 ID:SGTC_859|Compound:0439-0102|FD-Score:2.87|P-value:5.92E-4 ID:SGTC_869|Compound:0564-0080|FD-Score:4.82|P-value:3.24E-8 ID:SGTC_872|Compound:0588-0139|FD-Score:-3.37|P-value:1.06E-4 ID:SGTC_903|Compound:1134-0042|FD-Score:4.39|P-value:4.28E-7 ID:SGTC_955|Compound:1216-0110|FD-Score:-3.16|P-value:2.65E-4 ID:SGTC_1073|Compound:triclabendazole|FD-Score:3.12|P-value:2.20E-4 ID:SGTC_1086|Compound:cerivastatin|FD-Score:2.89|P-value:5.58E-4 ID:SGTC_1206|Compound:3978-0017|FD-Score:3.89|P-value:6.11E-6 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:-2.90|P-value:7.41E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.53|P-value:5.30E-5 ID:SGTC_1662|Compound:st014054|FD-Score:-2.90|P-value:7.41E-4 ID:SGTC_1699|Compound:st031318|FD-Score:-2.94|P-value:6.28E-4 ID:SGTC_1726|Compound:st035556|FD-Score:-4.13|P-value:2.78E-6 ID:SGTC_1739|Compound:st037196|FD-Score:3.95|P-value:4.53E-6 ID:SGTC_1742|Compound:st038235|FD-Score:-3.45|P-value:7.46E-5 ID:SGTC_1757|Compound:st041620|FD-Score:-3.11|P-value:3.28E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:3.01|P-value:3.43E-4 ID:SGTC_1879|Compound:gemcitabine|FD-Score:-3.73|P-value:2.03E-5 ID:SGTC_1884|Compound:5633444|FD-Score:2.88|P-value:5.80E-4 ID:SGTC_2048|Compound:5246248|FD-Score:3.42|P-value:5.98E-5 ID:SGTC_2051|Compound:5263410|FD-Score:-2.88|P-value:7.97E-4 ID:SGTC_2065|Compound:5221596|FD-Score:2.87|P-value:6.09E-4 ID:SGTC_2213|Compound:7173844|FD-Score:-3.04|P-value:4.26E-4 ID:SGTC_2351|Compound:9026858|FD-Score:4.68|P-value:7.66E-8 ID:SGTC_2405|Compound:ticlatone|FD-Score:3.25|P-value:1.27E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:4.08|P-value:2.34E-6 ID:SGTC_2436|Compound:5633522|FD-Score:2.84|P-value:6.87E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:-3.05|P-value:4.12E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-3.68|P-value:2.62E-5 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:3.18|P-value:1.69E-4 ID:SGTC_2591|Compound:skatole|FD-Score:4.69|P-value:7.35E-8 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:4.27|P-value:8.28E-7 ID:SGTC_2695|Compound:st077686|FD-Score:-3.47|P-value:6.79E-5 ID:SGTC_2709|Compound:st078560|FD-Score:3.93|P-value:4.92E-6 ID:SGTC_2710|Compound:st078562|FD-Score:-2.88|P-value:8.01E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.53|P-value:5.31E-5 ID:SGTC_3249|Compound:9135183|FD-Score:3.03|P-value:3.18E-4 ID:SGTC_3314|Compound:9135860|FD-Score:3.20|P-value:1.60E-4 ID:SGTC_3316|Compound:9136639|FD-Score:4.59|P-value:1.30E-7

Top fitness defect scores for YLR219W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_551 k048-0028 5.37 34.9 µM 9.13E-10 2 4.03E-8
2 SGTC_869 0564-0080 4.82 44.1 µM 3.24E-8 9 7.20E-7
3 SGTC_2591 skatole 4.69 100.0 µM 7.35E-8 19 1.39E-6
4 SGTC_2351 9026858 4.68 72.3 µM 7.66E-8 RNA processing & uracil transport 15 1.44E-6
5 SGTC_3316 9136639 4.59 10.6 µM 1.30E-7 7 2.21E-6
6 SGTC_369 0957-0393 4.56 4.5 µM 1.55E-7 cell wall signaling 10 2.54E-6
7 SGTC_903 1134-0042 4.39 114.0 µM 4.28E-7 15 5.79E-6
8 SGTC_2622 2-methoxyxanthone 4.27 59.9 µM 8.28E-7 13 9.88E-6
9 SGTC_500 h-89 4.20 112.0 µM 1.22E-6 cell wall signaling 26 1.35E-5
10 SGTC_2418 pentamidine 4.08 31.7 µM 2.34E-6 mitochondrial processes 37 2.29E-5
11 SGTC_1739 st037196 3.95 92.5 µM 4.53E-6 23 3.90E-5
12 SGTC_2709 st078560 3.93 56.0 µM 4.92E-6 12 4.18E-5
13 SGTC_1206 3978-0017 3.89 132.0 µM 6.11E-6 ERG2 39 4.97E-5
14 SGTC_839 0122-0028 3.80 230.0 µM 9.56E-6 RSC complex & mRNA processing 43 7.14E-5
15 SGTC_372 0155-0182 3.57 15.9 µM 2.90E-5 mitochondrial response to ROS 41 1.75E-4
16 SGTC_693 4161-2696 3.54 114.0 µM 3.34E-5 17 1.97E-4
17 SGTC_362 0310-0100 3.47 9.0 µM 4.82E-5 RPP1 & pyrimidine depletion 52 2.65E-4
18 SGTC_2048 5246248 3.42 200.0 µM 5.98E-5 16 3.15E-4
19 SGTC_133 0724-0033 3.34 378.9 µM 8.65E-5 48 4.25E-4
20 SGTC_2405 ticlatone 3.25 3.5 µM 1.27E-4 110 5.82E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.117 9.68E-12 YOR282W_d YOR282W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.105 9.04E-10 YKR018C YKR018C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication
0.084 1.09E-6 YER008C SEC3 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
0.082 2.24E-6 YLR024C UBR2 hom Cytoplasmic ubiquitin-protein ligase (E3); required for ubiquitylation of Rpn4p; mediates formation of a Mub1p-Ubr2p-Rad6p complex
0.081 2.41E-6 YPR008W HAA1 hom Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress
0.081 2.50E-6 YHL040C ARN1 hom ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress
0.080 3.24E-6 YAL008W FUN14 hom Mitochondrial protein of unknown function
0.080 3.29E-6 YEL012W UBC8 hom Ubiquitin-conjugating enzyme that negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro
0.080 4.08E-6 YER049W TPA1 hom Poly(rA)-binding protein involved in translation termination efficiency, mRNA poly(A) tail length and mRNA stability; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to prolyl 4-hydroxylases; binds Fe(III) and 2-oxoglutarate
0.079 4.44E-6 YHR014W SPO13 hom Meiosis-specific protein, involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II
0.079 4.61E-6 YFL051C_p YFL051C_p hom Putative protein of unknown function; YFL051C is not an essential gene
0.079 5.00E-6 YDR341C YDR341C het Arginyl-tRNA synthetase, proposed to be cytoplasmic but the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.077 7.22E-6 YBR136W MEC1 het Genome integrity checkpoint protein and PI kinase superfamily member; signal transducer required for cell cycle arrest and transcriptional responses prompted by damaged or unreplicated DNA; regulates P-body formation induced by replication stress; monitors and participates in meiotic recombination; associates with shortened, dysfunctional telomeres
0.077 8.10E-6 YOL151W GRE2 hom 3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase (D-lactaldehyde dehydrogenase); stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress
0.077 9.07E-6 YDR009W GAL3 hom Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication