YLR220W / CCC1

Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack the ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to the vacuole decreases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR220W / CCC1

Click on Significant Values for Screen Details ID:SGTC_132|Compound:0417-1715|FD-Score:3.41|P-value:1.93E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:3.29|P-value:3.09E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:-3.53|P-value:1.20E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:-3.62|P-value:8.49E-5 ID:SGTC_858|Compound:0438-0351|FD-Score:-3.05|P-value:7.59E-4 ID:SGTC_891|Compound:0929-0073|FD-Score:-3.94|P-value:2.15E-5 ID:SGTC_1046|Compound:2001-0062|FD-Score:3.47|P-value:1.52E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:3.98|P-value:1.77E-5 ID:SGTC_1075|Compound:aripiprazole|FD-Score:2.99|P-value:9.30E-4 ID:SGTC_1112|Compound:tnp00069|FD-Score:3.43|P-value:1.82E-4 ID:SGTC_1497|Compound:4476-2978|FD-Score:3.54|P-value:1.15E-4 ID:SGTC_1658|Compound:st012944|FD-Score:4.48|P-value:1.56E-6 ID:SGTC_1678|Compound:st019265|FD-Score:3.46|P-value:1.62E-4 ID:SGTC_1899|Compound:st063254|FD-Score:4.61|P-value:8.30E-7 ID:SGTC_2021|Compound:4019055|FD-Score:3.46|P-value:1.63E-4 ID:SGTC_2074|Compound:5212007|FD-Score:3.27|P-value:3.34E-4 ID:SGTC_2082|Compound:5219693|FD-Score:3.20|P-value:4.38E-4 ID:SGTC_2148|Compound:5546263|FD-Score:3.79|P-value:4.12E-5 ID:SGTC_2291|Compound:7917025|FD-Score:4.97|P-value:1.16E-7 ID:SGTC_2295|Compound:7971645|FD-Score:5.86|P-value:5.58E-10 ID:SGTC_2399|Compound:5884133|FD-Score:3.15|P-value:5.19E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:4.15|P-value:7.82E-6 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.63|P-value:7.96E-5 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:3.13|P-value:5.65E-4 ID:SGTC_2654|Compound:protoveratrine a|FD-Score:3.02|P-value:8.55E-4 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.13|P-value:5.68E-4 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:4.53|P-value:1.21E-6 ID:SGTC_2684|Compound:capsaicin|FD-Score:3.15|P-value:5.20E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:-3.26|P-value:3.52E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:3.33|P-value:2.62E-4 ID:SGTC_2782|Compound:7736369|FD-Score:3.10|P-value:6.24E-4 ID:SGTC_2852|Compound:9023854|FD-Score:4.09|P-value:1.07E-5 ID:SGTC_2970|Compound:9090938|FD-Score:-3.29|P-value:3.16E-4 ID:SGTC_2983|Compound:9022671|FD-Score:-3.44|P-value:1.76E-4 ID:SGTC_3006|Compound:9075424|FD-Score:3.04|P-value:7.84E-4 ID:SGTC_3223|Compound:9129970|FD-Score:4.20|P-value:6.37E-6 ID:SGTC_132|Compound:0417-1715|FD-Score:3.41|P-value:1.93E-4 ID:SGTC_139|Compound:0099-0334|FD-Score:3.29|P-value:3.09E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:-3.53|P-value:1.20E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:-3.62|P-value:8.49E-5 ID:SGTC_858|Compound:0438-0351|FD-Score:-3.05|P-value:7.59E-4 ID:SGTC_891|Compound:0929-0073|FD-Score:-3.94|P-value:2.15E-5 ID:SGTC_1046|Compound:2001-0062|FD-Score:3.47|P-value:1.52E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:3.98|P-value:1.77E-5 ID:SGTC_1075|Compound:aripiprazole|FD-Score:2.99|P-value:9.30E-4 ID:SGTC_1112|Compound:tnp00069|FD-Score:3.43|P-value:1.82E-4 ID:SGTC_1497|Compound:4476-2978|FD-Score:3.54|P-value:1.15E-4 ID:SGTC_1658|Compound:st012944|FD-Score:4.48|P-value:1.56E-6 ID:SGTC_1678|Compound:st019265|FD-Score:3.46|P-value:1.62E-4 ID:SGTC_1899|Compound:st063254|FD-Score:4.61|P-value:8.30E-7 ID:SGTC_2021|Compound:4019055|FD-Score:3.46|P-value:1.63E-4 ID:SGTC_2074|Compound:5212007|FD-Score:3.27|P-value:3.34E-4 ID:SGTC_2082|Compound:5219693|FD-Score:3.20|P-value:4.38E-4 ID:SGTC_2148|Compound:5546263|FD-Score:3.79|P-value:4.12E-5 ID:SGTC_2291|Compound:7917025|FD-Score:4.97|P-value:1.16E-7 ID:SGTC_2295|Compound:7971645|FD-Score:5.86|P-value:5.58E-10 ID:SGTC_2399|Compound:5884133|FD-Score:3.15|P-value:5.19E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:4.15|P-value:7.82E-6 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.63|P-value:7.96E-5 ID:SGTC_2633|Compound:5,7-dihydroxyisoflavone|FD-Score:3.13|P-value:5.65E-4 ID:SGTC_2654|Compound:protoveratrine a|FD-Score:3.02|P-value:8.55E-4 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.13|P-value:5.68E-4 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:4.53|P-value:1.21E-6 ID:SGTC_2684|Compound:capsaicin|FD-Score:3.15|P-value:5.20E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:-3.26|P-value:3.52E-4 ID:SGTC_2767|Compound:sibutramine|FD-Score:3.33|P-value:2.62E-4 ID:SGTC_2782|Compound:7736369|FD-Score:3.10|P-value:6.24E-4 ID:SGTC_2852|Compound:9023854|FD-Score:4.09|P-value:1.07E-5 ID:SGTC_2970|Compound:9090938|FD-Score:-3.29|P-value:3.16E-4 ID:SGTC_2983|Compound:9022671|FD-Score:-3.44|P-value:1.76E-4 ID:SGTC_3006|Compound:9075424|FD-Score:3.04|P-value:7.84E-4 ID:SGTC_3223|Compound:9129970|FD-Score:4.20|P-value:6.37E-6

Top fitness defect scores for YLR220W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2295 7971645 5.86 200.0 µM 5.58E-10 6 2.34E-9
2 SGTC_2291 7917025 4.97 104.3 µM 1.16E-7 iron homeostasis 29 3.29E-7
3 SGTC_1899 st063254 4.61 59.6 µM 8.30E-7 6 2.04E-6
4 SGTC_2675 theaflavin digallate 4.53 100.0 µM 1.21E-6 iron homeostasis 21 2.90E-6
5 SGTC_1658 st012944 4.48 20.0 µM 1.56E-6 iron homeostasis 62 3.67E-6
6 SGTC_3223 9129970 4.20 49.5 µM 6.37E-6 21 1.35E-5
7 SGTC_2418 pentamidine 4.15 31.7 µM 7.82E-6 mitochondrial processes 36 1.64E-5
8 SGTC_2852 9023854 4.09 52.0 µM 1.07E-5 31 2.18E-5
9 SGTC_1049 3226-0134 3.98 66.0 µM 1.77E-5 27 3.49E-5
10 SGTC_2148 5546263 3.79 113.6 µM 4.12E-5 26 7.66E-5
11 SGTC_2621 lithochol-11-enic acid 3.63 100.0 µM 7.96E-5 37 1.41E-4
12 SGTC_1497 4476-2978 3.54 41.3 µM 1.15E-4 19 1.98E-4
13 SGTC_1046 2001-0062 3.47 216.0 µM 1.52E-4 30 2.57E-4
14 SGTC_1678 st019265 3.46 80.2 µM 1.62E-4 60S ribosome export 37 2.73E-4
15 SGTC_2021 4019055 3.46 173.0 µM 1.63E-4 endomembrane recycling 164 2.74E-4
16 SGTC_1112 tnp00069 3.43 1.2 µM 1.82E-4 43 3.04E-4
17 SGTC_132 0417-1715 3.41 25.8 µM 1.93E-4 RPP1 & pyrimidine depletion 35 3.21E-4
18 SGTC_2767 sibutramine 3.33 71.5 µM 2.62E-4 37 4.28E-4
19 SGTC_139 0099-0334 3.29 718.6 µM 3.09E-4 62 4.98E-4
20 SGTC_2074 5212007 3.27 75.4 µM 3.34E-4 30 5.35E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.256 3.73E-51 YMR058W FET3 hom Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress
0.221 2.93E-38 YDR270W CCC2 hom Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism
0.192 3.04E-29 YDR271C_d YDR271C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W
0.173 5.89E-24 YOR382W FIT2 hom Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
0.158 3.71E-20 YKL099C UTP11 het Subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit
0.157 5.44E-20 YDR269C_d YDR269C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.142 1.35E-16 YKR018C YKR018C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication
0.141 1.94E-16 YER145C FTR1 hom High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress
0.141 2.15E-16 YDR203W_d YDR203W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.129 5.38E-14 YJR040W GEF1 hom Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism
0.102 3.48E-9 YPR148C YPR148C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.101 4.37E-9 YJL094C KHA1 hom Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies
0.094 4.48E-8 YDR202C RAV2 hom Subunit of RAVE (Rav1p, Rav2p, Skp1p), a complex that associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme
0.093 6.56E-8 YPL198W RPL7B hom Ribosomal 60S subunit protein L7B; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication
0.093 6.58E-8 YJR033C RAV1 hom Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate