YLR221C / RSA3

Protein with a likely role in ribosomal maturation, required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR221C / RSA3

Click on Significant Values for Screen Details ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:5.90|P-value:1.30E-9 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:3.69|P-value:9.21E-5 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.48|P-value:2.09E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.55|P-value:2.15E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:-3.64|P-value:1.50E-4 ID:SGTC_375|Compound:3970-0961|FD-Score:-5.53|P-value:1.75E-8 ID:SGTC_378|Compound:3970-0959|FD-Score:3.33|P-value:3.75E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:3.30|P-value:4.06E-4 ID:SGTC_385|Compound:1494-0393|FD-Score:4.71|P-value:9.66E-7 ID:SGTC_391|Compound:gliotoxin|FD-Score:-4.15|P-value:1.85E-5 ID:SGTC_430|Compound:0958-0115|FD-Score:-3.20|P-value:7.45E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:3.65|P-value:1.10E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:-3.26|P-value:6.14E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:4.30|P-value:6.87E-6 ID:SGTC_703|Compound:3875-0075|FD-Score:3.41|P-value:2.79E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:3.67|P-value:9.92E-5 ID:SGTC_708|Compound:3448-5400|FD-Score:-5.79|P-value:3.84E-9 ID:SGTC_815|Compound:1582-0027|FD-Score:-3.41|P-value:3.54E-4 ID:SGTC_979|Compound:1426-2828|FD-Score:3.44|P-value:2.48E-4 ID:SGTC_1042|Compound:0350-0096|FD-Score:-3.65|P-value:1.42E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:4.27|P-value:7.76E-6 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.13|P-value:7.60E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:-3.22|P-value:7.03E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:-3.25|P-value:6.26E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.19|P-value:6.17E-4 ID:SGTC_1570|Compound:verbenone|FD-Score:3.06|P-value:9.47E-4 ID:SGTC_2297|Compound:7971671|FD-Score:4.51|P-value:2.59E-6 ID:SGTC_2320|Compound:7373003|FD-Score:-3.30|P-value:5.37E-4 ID:SGTC_2323|Compound:6148992|FD-Score:-3.42|P-value:3.42E-4 ID:SGTC_2333|Compound:9017922|FD-Score:3.44|P-value:2.50E-4 ID:SGTC_2455|Compound:5377712|FD-Score:3.34|P-value:3.53E-4 ID:SGTC_2488|Compound:5268135|FD-Score:3.17|P-value:6.61E-4 ID:SGTC_2491|Compound:paeonol|FD-Score:4.18|P-value:1.18E-5 ID:SGTC_2610|Compound:ononetin|FD-Score:3.20|P-value:5.92E-4 ID:SGTC_2702|Compound:st077768|FD-Score:-3.12|P-value:9.99E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:5.69|P-value:4.60E-9 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.18|P-value:6.33E-4 ID:SGTC_2794|Compound:7785382|FD-Score:4.29|P-value:7.30E-6 ID:SGTC_2800|Compound:7887935|FD-Score:-3.78|P-value:8.81E-5 ID:SGTC_3108|Compound:9121785|FD-Score:3.41|P-value:2.78E-4 ID:SGTC_3201|Compound:9113999|FD-Score:6.33|P-value:8.57E-11 ID:SGTC_3208|Compound:9114115|FD-Score:-5.45|P-value:2.77E-8 ID:SGTC_3348|Compound:9149464|FD-Score:-4.14|P-value:1.89E-5 ID:SGTC_239|Compound:5hr heat shock (37°C) + mitomycin C|FD-Score:5.90|P-value:1.30E-9 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:3.69|P-value:9.21E-5 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:3.48|P-value:2.09E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.55|P-value:2.15E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:-3.64|P-value:1.50E-4 ID:SGTC_375|Compound:3970-0961|FD-Score:-5.53|P-value:1.75E-8 ID:SGTC_378|Compound:3970-0959|FD-Score:3.33|P-value:3.75E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:3.30|P-value:4.06E-4 ID:SGTC_385|Compound:1494-0393|FD-Score:4.71|P-value:9.66E-7 ID:SGTC_391|Compound:gliotoxin|FD-Score:-4.15|P-value:1.85E-5 ID:SGTC_430|Compound:0958-0115|FD-Score:-3.20|P-value:7.45E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:3.65|P-value:1.10E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:-3.26|P-value:6.14E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:4.30|P-value:6.87E-6 ID:SGTC_703|Compound:3875-0075|FD-Score:3.41|P-value:2.79E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:3.67|P-value:9.92E-5 ID:SGTC_708|Compound:3448-5400|FD-Score:-5.79|P-value:3.84E-9 ID:SGTC_815|Compound:1582-0027|FD-Score:-3.41|P-value:3.54E-4 ID:SGTC_979|Compound:1426-2828|FD-Score:3.44|P-value:2.48E-4 ID:SGTC_1042|Compound:0350-0096|FD-Score:-3.65|P-value:1.42E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:4.27|P-value:7.76E-6 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.13|P-value:7.60E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:-3.22|P-value:7.03E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:-3.25|P-value:6.26E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.19|P-value:6.17E-4 ID:SGTC_1570|Compound:verbenone|FD-Score:3.06|P-value:9.47E-4 ID:SGTC_2297|Compound:7971671|FD-Score:4.51|P-value:2.59E-6 ID:SGTC_2320|Compound:7373003|FD-Score:-3.30|P-value:5.37E-4 ID:SGTC_2323|Compound:6148992|FD-Score:-3.42|P-value:3.42E-4 ID:SGTC_2333|Compound:9017922|FD-Score:3.44|P-value:2.50E-4 ID:SGTC_2455|Compound:5377712|FD-Score:3.34|P-value:3.53E-4 ID:SGTC_2488|Compound:5268135|FD-Score:3.17|P-value:6.61E-4 ID:SGTC_2491|Compound:paeonol|FD-Score:4.18|P-value:1.18E-5 ID:SGTC_2610|Compound:ononetin|FD-Score:3.20|P-value:5.92E-4 ID:SGTC_2702|Compound:st077768|FD-Score:-3.12|P-value:9.99E-4 ID:SGTC_2753|Compound:fluvastatin|FD-Score:5.69|P-value:4.60E-9 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.18|P-value:6.33E-4 ID:SGTC_2794|Compound:7785382|FD-Score:4.29|P-value:7.30E-6 ID:SGTC_2800|Compound:7887935|FD-Score:-3.78|P-value:8.81E-5 ID:SGTC_3108|Compound:9121785|FD-Score:3.41|P-value:2.78E-4 ID:SGTC_3201|Compound:9113999|FD-Score:6.33|P-value:8.57E-11 ID:SGTC_3208|Compound:9114115|FD-Score:-5.45|P-value:2.77E-8 ID:SGTC_3348|Compound:9149464|FD-Score:-4.14|P-value:1.89E-5

Top fitness defect scores for YLR221C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3201 9113999 6.33 49.5 µM 8.57E-11 4 1.22E-10
2 SGTC_239 5hr heat shock (37°C) + mitomycin C 5.90 819.4 µM 1.30E-9 heat shock/prefoldin 11 1.79E-9
3 SGTC_2753 fluvastatin 5.69 17.1 µM 4.60E-9 azole & statin 8 6.27E-9
4 SGTC_385 1494-0393 4.71 12.4 µM 9.66E-7 8 1.23E-6
5 SGTC_2297 7971671 4.51 152.3 µM 2.59E-6 RNA processing & uracil transport 15 3.26E-6
6 SGTC_627 1391-0720 4.30 175.0 µM 6.87E-6 7 8.55E-6
7 SGTC_2794 7785382 4.29 27.3 µM 7.30E-6 25 9.08E-6
8 SGTC_1213 1315-0166 4.27 115.0 µM 7.76E-6 20 9.65E-6
9 SGTC_2491 paeonol 4.18 100.0 µM 1.18E-5 7 1.46E-5
10 SGTC_240 5hr heat shock (37°C) + Streptovitacin 3.69 300.0 µM 9.21E-5 heat shock/prefoldin 49 1.11E-4
11 SGTC_705 1124-0212 3.67 310.0 µM 9.92E-5 23 1.19E-4
12 SGTC_543 3844-1095 3.65 191.0 µM 1.10E-4 ERG2 60 1.33E-4
13 SGTC_261 5-fluorouridine 3.48 15.4 µM 2.09E-4 exosome 26 2.49E-4
14 SGTC_979 1426-2828 3.44 121.0 µM 2.48E-4 34 2.94E-4
15 SGTC_2333 9017922 3.44 200.0 µM 2.50E-4 42 2.96E-4
16 SGTC_3108 9121785 3.41 49.5 µM 2.78E-4 32 3.29E-4
17 SGTC_703 3875-0075 3.41 71.8 µM 2.79E-4 16 3.30E-4
18 SGTC_2455 5377712 3.34 9.8 µM 3.53E-4 41 4.17E-4
19 SGTC_378 3970-0959 3.33 8.3 µM 3.75E-4 32 4.42E-4
20 SGTC_382 3229-0966 3.30 23.2 µM 4.06E-4 45 4.78E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.125 4.30E-13 YHL016C DUR3 hom Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway
0.106 7.19E-10 YDR457W TOM1 hom E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
0.105 1.07E-9 YDR260C SWM1 hom Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
0.087 4.68E-7 YLR117C CLF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.087 5.01E-7 YML075C HMG1 hom HMG-CoA reductase; catalyzes the conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to the nuclear envelope; overproduction induces the formation of karmellae; forms foci at the nuclear periphery upon DNA replication stress
0.086 6.23E-7 YLR027C AAT2 hom Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.084 1.09E-6 YDL039C PRM7 hom Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements
0.084 1.11E-6 YLL063C AYT1 hom Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis
0.084 1.17E-6 YMR132C_p JLP2_p hom Protein of unknown function, contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)
0.083 1.42E-6 YOR213C SAS5 hom Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity
0.083 1.48E-6 YKR024C DBP7 hom Putative ATP-dependent RNA helicase of the DEAD-box family involved in ribosomal biogenesis; essential for growth under anaerobic conditions
0.080 3.03E-6 YGL037C PNC1 hom Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress
0.080 3.80E-6 YPR200C ARR2 hom Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p
0.079 5.14E-6 YDR430C CYM1 hom Lysine-specific metalloprotease of the mitochondrial intermembrane space, member of the pitrilysin family; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology
0.078 5.91E-6 YBL077W_d YBL077W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ILS1/YBL076C