YLR228C / ECM22

Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR228C / ECM22

Click on Significant Values for Screen Details ID:SGTC_33|Compound:0986-0033|FD-Score:5.12|P-value:7.04E-8 ID:SGTC_36|Compound:0335-0881|FD-Score:-3.82|P-value:6.25E-5 ID:SGTC_65|Compound:0798-0905|FD-Score:5.74|P-value:1.92E-9 ID:SGTC_279|Compound:1273-0055|FD-Score:3.29|P-value:3.32E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:7.70|P-value:1.57E-15 ID:SGTC_350|Compound:k064-0020|FD-Score:4.70|P-value:6.55E-7 ID:SGTC_363|Compound:0455-0021|FD-Score:7.38|P-value:1.92E-14 ID:SGTC_447|Compound:ml7|FD-Score:4.43|P-value:2.59E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:3.20|P-value:4.73E-4 ID:SGTC_578|Compound:3460-0051|FD-Score:-3.83|P-value:5.98E-5 ID:SGTC_585|Compound:0kpi-0023|FD-Score:-4.01|P-value:2.77E-5 ID:SGTC_658|Compound:3253-0951|FD-Score:4.31|P-value:4.46E-6 ID:SGTC_768|Compound:0384-0033|FD-Score:3.43|P-value:1.96E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:-3.23|P-value:6.16E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:4.23|P-value:6.66E-6 ID:SGTC_789|Compound:1309-1135|FD-Score:-3.72|P-value:9.33E-5 ID:SGTC_862|Compound:0452-0056|FD-Score:4.99|P-value:1.40E-7 ID:SGTC_993|Compound:3909-8757|FD-Score:-3.30|P-value:4.67E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.87|P-value:3.24E-5 ID:SGTC_1041|Compound:0206-0046|FD-Score:3.20|P-value:4.68E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.86|P-value:1.67E-9 ID:SGTC_1111|Compound:tnp00062|FD-Score:-3.20|P-value:6.89E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.27|P-value:3.61E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.04|P-value:8.26E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.70|P-value:6.63E-5 ID:SGTC_1266|Compound:0828-0227|FD-Score:3.49|P-value:1.59E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.18|P-value:5.05E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:3.02|P-value:8.77E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.70|P-value:6.76E-5 ID:SGTC_1509|Compound:1326-1131|FD-Score:3.31|P-value:3.07E-4 ID:SGTC_1547|Compound:harmine|FD-Score:4.62|P-value:1.01E-6 ID:SGTC_1712|Compound:st032288|FD-Score:4.99|P-value:1.45E-7 ID:SGTC_1787|Compound:fluconazole|FD-Score:4.97|P-value:1.58E-7 ID:SGTC_1816|Compound:st051957|FD-Score:-3.19|P-value:6.96E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.08|P-value:2.07E-5 ID:SGTC_1935|Compound:st074532|FD-Score:3.31|P-value:3.07E-4 ID:SGTC_1943|Compound:st076331|FD-Score:-3.44|P-value:2.84E-4 ID:SGTC_1947|Compound:st076332|FD-Score:-4.24|P-value:1.01E-5 ID:SGTC_2021|Compound:4019055|FD-Score:3.55|P-value:1.23E-4 ID:SGTC_2114|Compound:5491910|FD-Score:4.65|P-value:8.48E-7 ID:SGTC_2119|Compound:5528445|FD-Score:6.77|P-value:1.89E-12 ID:SGTC_2288|Compound:7946289|FD-Score:3.32|P-value:3.02E-4 ID:SGTC_2444|Compound:5485484|FD-Score:3.17|P-value:5.18E-4 ID:SGTC_2461|Compound:5755302|FD-Score:3.18|P-value:5.08E-4 ID:SGTC_2469|Compound:5655019|FD-Score:3.15|P-value:5.57E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-3.59|P-value:1.62E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.21|P-value:6.52E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:5.39|P-value:1.54E-8 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-3.33|P-value:4.33E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.11|P-value:6.37E-4 ID:SGTC_3152|Compound:9099253|FD-Score:4.24|P-value:6.14E-6 ID:SGTC_33|Compound:0986-0033|FD-Score:5.12|P-value:7.04E-8 ID:SGTC_36|Compound:0335-0881|FD-Score:-3.82|P-value:6.25E-5 ID:SGTC_65|Compound:0798-0905|FD-Score:5.74|P-value:1.92E-9 ID:SGTC_279|Compound:1273-0055|FD-Score:3.29|P-value:3.32E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:7.70|P-value:1.57E-15 ID:SGTC_350|Compound:k064-0020|FD-Score:4.70|P-value:6.55E-7 ID:SGTC_363|Compound:0455-0021|FD-Score:7.38|P-value:1.92E-14 ID:SGTC_447|Compound:ml7|FD-Score:4.43|P-value:2.59E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:3.20|P-value:4.73E-4 ID:SGTC_578|Compound:3460-0051|FD-Score:-3.83|P-value:5.98E-5 ID:SGTC_585|Compound:0kpi-0023|FD-Score:-4.01|P-value:2.77E-5 ID:SGTC_658|Compound:3253-0951|FD-Score:4.31|P-value:4.46E-6 ID:SGTC_768|Compound:0384-0033|FD-Score:3.43|P-value:1.96E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:-3.23|P-value:6.16E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:4.23|P-value:6.66E-6 ID:SGTC_789|Compound:1309-1135|FD-Score:-3.72|P-value:9.33E-5 ID:SGTC_862|Compound:0452-0056|FD-Score:4.99|P-value:1.40E-7 ID:SGTC_993|Compound:3909-8757|FD-Score:-3.30|P-value:4.67E-4 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.87|P-value:3.24E-5 ID:SGTC_1041|Compound:0206-0046|FD-Score:3.20|P-value:4.68E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.86|P-value:1.67E-9 ID:SGTC_1111|Compound:tnp00062|FD-Score:-3.20|P-value:6.89E-4 ID:SGTC_1118|Compound:chelidonine|FD-Score:3.27|P-value:3.61E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.04|P-value:8.26E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.70|P-value:6.63E-5 ID:SGTC_1266|Compound:0828-0227|FD-Score:3.49|P-value:1.59E-4 ID:SGTC_1317|Compound:1273-0028|FD-Score:3.18|P-value:5.05E-4 ID:SGTC_1346|Compound:1488-2224|FD-Score:3.02|P-value:8.77E-4 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.70|P-value:6.76E-5 ID:SGTC_1509|Compound:1326-1131|FD-Score:3.31|P-value:3.07E-4 ID:SGTC_1547|Compound:harmine|FD-Score:4.62|P-value:1.01E-6 ID:SGTC_1712|Compound:st032288|FD-Score:4.99|P-value:1.45E-7 ID:SGTC_1787|Compound:fluconazole|FD-Score:4.97|P-value:1.58E-7 ID:SGTC_1816|Compound:st051957|FD-Score:-3.19|P-value:6.96E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-4.08|P-value:2.07E-5 ID:SGTC_1935|Compound:st074532|FD-Score:3.31|P-value:3.07E-4 ID:SGTC_1943|Compound:st076331|FD-Score:-3.44|P-value:2.84E-4 ID:SGTC_1947|Compound:st076332|FD-Score:-4.24|P-value:1.01E-5 ID:SGTC_2021|Compound:4019055|FD-Score:3.55|P-value:1.23E-4 ID:SGTC_2114|Compound:5491910|FD-Score:4.65|P-value:8.48E-7 ID:SGTC_2119|Compound:5528445|FD-Score:6.77|P-value:1.89E-12 ID:SGTC_2288|Compound:7946289|FD-Score:3.32|P-value:3.02E-4 ID:SGTC_2444|Compound:5485484|FD-Score:3.17|P-value:5.18E-4 ID:SGTC_2461|Compound:5755302|FD-Score:3.18|P-value:5.08E-4 ID:SGTC_2469|Compound:5655019|FD-Score:3.15|P-value:5.57E-4 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-3.59|P-value:1.62E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.21|P-value:6.52E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:5.39|P-value:1.54E-8 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-3.33|P-value:4.33E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.11|P-value:6.37E-4 ID:SGTC_3152|Compound:9099253|FD-Score:4.24|P-value:6.14E-6

Top fitness defect scores for YLR228C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_345 1362-0200 7.70 395.0 µM 1.57E-15 2 7.03E-15
2 SGTC_363 0455-0021 7.38 3.1 µM 1.92E-14 azole & statin 18 7.75E-14
3 SGTC_2119 5528445 6.77 120.6 µM 1.89E-12 1 6.33E-12
4 SGTC_65 0798-0905 5.74 35.0 µM 1.92E-9 calcium & mitochondrial duress 38 4.84E-9
5 SGTC_2620 irigenin 5.39 100.0 µM 1.54E-8 10 3.55E-8
6 SGTC_33 0986-0033 5.12 35.8 µM 7.04E-8 calcium & mitochondrial duress 10 1.52E-7
7 SGTC_862 0452-0056 4.99 10.2 µM 1.40E-7 azole & statin 23 2.95E-7
8 SGTC_1712 st032288 4.99 53.4 µM 1.45E-7 calcium & mitochondrial duress 121 3.05E-7
9 SGTC_1787 fluconazole 4.97 80.0 µM 1.58E-7 iron homeostasis 10 3.30E-7
10 SGTC_350 k064-0020 4.70 19.8 µM 6.55E-7 heme requiring 16 1.29E-6
11 SGTC_2114 5491910 4.65 81.2 µM 8.48E-7 3 1.65E-6
12 SGTC_1547 harmine 4.62 94.2 µM 1.01E-6 ergosterol biosynthesis 27 1.95E-6
13 SGTC_447 ml7 4.43 120.0 µM 2.59E-6 NEO1-PIK1 21 4.81E-6
14 SGTC_658 3253-0951 4.31 102.0 µM 4.46E-6 calcium & mitochondrial duress 48 8.08E-6
15 SGTC_3152 9099253 4.24 49.5 µM 6.14E-6 NEO1-PIK1 113 1.10E-5
16 SGTC_778 3456-2434 4.23 196.0 µM 6.66E-6 mitochondrial response to ROS 67 1.19E-5
17 SGTC_1038 k292-0204 3.87 154.0 µM 3.24E-5 19 5.37E-5
18 SGTC_1254 0550-0035 3.70 264.0 µM 6.63E-5 29 1.07E-4
19 SGTC_1350 1495-0454 3.70 146.0 µM 6.76E-5 39 1.09E-4
20 SGTC_2021 4019055 3.55 173.0 µM 1.23E-4 endomembrane recycling 151 1.93E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.189 2.06E-28 YOR275C RIM20 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation
0.185 2.83E-27 YBL046W PSY4 hom Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2
0.177 6.48E-25 YGR122W YGR122W hom Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
0.174 3.19E-24 YMR154C RIM13 hom Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
0.169 7.87E-23 YGR170W PSD2 hom Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine
0.157 7.13E-20 YHR072W ERG7 het Lanosterol synthase, an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis
0.157 7.34E-20 YGR004W PEX31 hom Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p
0.154 2.29E-19 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.151 1.19E-18 YGL045W RIM8 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
0.144 4.67E-17 YNL294C RIM21 hom pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH
0.142 1.70E-16 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.138 9.59E-16 YOR030W DFG16 hom Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p
0.132 1.62E-14 YPL145C KES1 hom One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
0.131 3.00E-14 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.130 4.04E-14 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data