HIPHOP chemogenomics database

YLR232W_d

Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5

Zygosity: Homozygous strain

fixedexpanded
Profile for YLR232W / YLR232W

Click on Significant Values for Screen Details

Top fitness defect scores for YLR232W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_58	0957-0393	8.58	9.0 µM	1.88E-20	cell wall	109	4.75E-18
2	SGTC_2396	5342950	6.12	42.8 µM	2.73E-11	RSC complex & mRNA processing	13	4.73E-10
3	SGTC_2784	7567673	5.97	72.7 µM	7.71E-11	iron homeostasis	6	1.17E-9
4	SGTC_103	0844-0104	5.89	51.8 µM	1.36E-10	ERAD & cell cycle	79	1.92E-9
5	SGTC_2143	5623677	4.22	140.5 µM	3.02E-6		2	1.22E-5
6	SGTC_138	0850-0259	4.14	40.9 µM	4.56E-6	cell wall	189	1.75E-5
7	SGTC_1717	st033143	4.09	58.0 µM	5.69E-6		37	2.12E-5
8	SGTC_1607	st000305	3.84	50.6 µM	1.88E-5		15	6.04E-5
9	SGTC_116	0155-0182	3.82	31.8 µM	2.11E-5	mitochondrial response to ROS	89	6.69E-5
10	SGTC_2149	5554786	3.72	194.8 µM	3.34E-5	cell wall signaling	45	1.00E-4
11	SGTC_1707	st032323	3.64	6.3 µM	4.78E-5	DNA damage response	70	1.37E-4
12	SGTC_1616	st002734	3.58	56.1 µM	6.25E-5		42	1.73E-4
13	SGTC_1791	st048313	3.48	56.1 µM	9.38E-5		30	2.48E-4
14	SGTC_373	0844-0104	3.43	25.9 µM	1.17E-4	ERAD & cell cycle	73	3.01E-4
15	SGTC_2310	7668677	3.37	200.0 µM	1.49E-4		59	3.71E-4
16	SGTC_1218	1348-1416	3.37	30.7 µM	1.50E-4	SWF1 & branched chain AA biosynthesis	34	3.73E-4
17	SGTC_2309	7664088	3.37	163.9 µM	1.52E-4		73	3.78E-4
18	SGTC_2004	4023728	3.34	41.9 µM	1.69E-4		53	4.16E-4
19	SGTC_2335	9018011	3.32	154.2 µM	1.84E-4		46	4.46E-4
20	SGTC_2009	4012297	3.25	12.1 µM	2.44E-4	cell wall signaling	97	5.74E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.248	2.43E-48	YKR042W	UTH1	hom	Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
0.246	2.33E-47	YOR231W	MKK1	hom	MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.237	5.94E-44	YDR205W	MSC2	hom	Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.221	2.12E-38	YAL015C	NTG1	hom	DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.220	5.86E-38	YLR150W	STM1	hom	Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.215	2.78E-36	YGR058W	PEF1	hom	Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.214	4.03E-36	YBR260C	RGD1	hom	GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.206	1.48E-33	YJR054W	KCH1	hom	Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.203	1.52E-32	YKL115C_d	YKL115C_d	hom	Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
0.200	1.09E-31	YDL203C	ACK1	hom	Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.196	1.70E-30	YNL179C_d	YNL179C_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance
0.196	2.15E-30	YDR335W	MSN5	hom	Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm
0.196	2.18E-30	YDR537C_d	YDR537C_d	hom	Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W
0.196	2.48E-30	YLR331C_d	JIP3_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.192	4.24E-29	YOR051C	ETT1	hom	Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough