YLR243W / GPN3

Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR243W / GPN3

Click on Significant Values for Screen Details

Top fitness defect scores for YLR243W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_210 3551-0445 14.30 56.8 µM 1.94E-60 2 1.51E-46 6.11
2 SGTC_2004 4023728 14.10 41.9 µM 8.62E-59 2 2.76E-45 0.33
3 SGTC_611 3453-1652 13.30 23.7 µM 4.82E-53 2 6.84E-41 4.50
4 SGTC_2466 5983409 10.20 37.1 µM 7.62E-32 1 1.10E-24 3.57
5 SGTC_2430 5571206 10.00 200.0 µM 6.08E-31 2 5.40E-24 2.06
6 SGTC_897 1000-0139 8.48 31.4 µM 1.12E-22 2 1.13E-17 0.84
7 SGTC_2233 sulbentine 8.25 40.4 µM 1.40E-21 15 7.82E-17 0.04
8 SGTC_2408 sulbentine 6.43 18.4 µM 7.19E-14 16 6.20E-11 0.17
9 SGTC_2780 5215298 5.91 71.4 µM 5.45E-12 5 1.70E-9 0.11
10 SGTC_2323 6148992 5.85 148.3 µM 8.51E-12 7 2.40E-9 0.59
11 SGTC_2480 5767542 5.59 37.2 µM 6.48E-11 1 1.13E-8 0.90
12 SGTC_2246 7293507 5.30 91.6 µM 5.57E-10 5 5.90E-8 0.01
13 SGTC_2921 7974240 4.77 10.7 µM 2.02E-8 10 9.27E-7 0.11
14 SGTC_2456 5770359 4.74 74.5 µM 2.38E-8 1 1.05E-6 0.45
15 SGTC_2151 5563267 4.65 85.4 µM 4.21E-8 13 1.63E-6 0.34
16 SGTC_589 1227-0070 4.63 34.1 µM 4.94E-8 6 1.84E-6 0.27
17 SGTC_2573 2',4'-dihydroxychalcone 4.54 15.0 µM 8.54E-8 9 2.80E-6 0.01
18 SGTC_2612 dictamnine 4.48 15.5 µM 1.27E-7 RNA pol III & RNase P/MRP 23 3.80E-6 0.01
19 SGTC_2470 5664817 4.26 21.7 µM 4.59E-7 3 1.02E-5 0.35
20 SGTC_3317 9138495 3.88 8.8 µM 3.94E-6 11 5.31E-5 0.14

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.452 2.25E-168 YMR185W RTP1 het Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
0.449 5.45E-166 YDR527W RBA50 het Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
0.424 2.34E-146 YOR207C RET1 het Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.319 7.54E-80 YAL024C LTE1 hom Protein similar to GDP/GTP exchange factors but without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
0.297 3.14E-69 YOR349W CIN1 hom Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl
0.282 2.42E-62 YOR262W GPN2 het Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B; required for establishment of sister chromatid cohesion
0.257 1.45E-51 YPL241C CIN2 hom GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2
0.250 6.62E-49 YDR041W RSM10 het Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
0.245 4.00E-47 YER007W PAC2 hom Microtubule effector required for tubulin heterodimer formation, binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
0.240 3.52E-45 YML124C TUB3 hom Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
0.230 2.08E-41 YNL298W CLA4 hom Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p
0.217 5.62E-37 YPR085C ASA1 het Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
0.211 5.02E-35 YNL112W DBP2 het ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
0.193 2.61E-29 YNL148C ALF1 hom Alpha-tubulin folding protein, similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
0.175 2.05E-24 YNR050C LYS9 hom Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112