YLR248W / RCK2

Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR248W / RCK2

Click on Significant Values for Screen Details ID:SGTC_48|Compound:0382-0018|FD-Score:-3.30|P-value:5.19E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:4.57|P-value:3.34E-6 ID:SGTC_104|Compound:0074-0074|FD-Score:3.43|P-value:3.65E-4 ID:SGTC_228|Compound:caffeine|FD-Score:-3.42|P-value:3.44E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:3.72|P-value:1.24E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:4.44|P-value:6.21E-6 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:3.20|P-value:8.17E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:5.07|P-value:3.00E-7 ID:SGTC_670|Compound:4204-0025|FD-Score:3.41|P-value:4.02E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:4.44|P-value:6.07E-6 ID:SGTC_942|Compound:3253-1115|FD-Score:3.34|P-value:5.08E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:-3.76|P-value:9.38E-5 ID:SGTC_1182|Compound:1222-0449|FD-Score:3.21|P-value:8.08E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.42|P-value:3.83E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.33|P-value:5.23E-4 ID:SGTC_1620|Compound:st003704|FD-Score:3.65|P-value:1.61E-4 ID:SGTC_1663|Compound:st014140|FD-Score:-3.96|P-value:4.21E-5 ID:SGTC_1721|Compound:st082152|FD-Score:-3.25|P-value:6.22E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:3.30|P-value:5.93E-4 ID:SGTC_1916|Compound:st067606|FD-Score:-3.97|P-value:4.10E-5 ID:SGTC_1970|Compound:st070122|FD-Score:3.27|P-value:6.45E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.53|P-value:2.56E-4 ID:SGTC_2047|Compound:4141938|FD-Score:3.33|P-value:5.26E-4 ID:SGTC_2123|Compound:5259487|FD-Score:3.51|P-value:2.72E-4 ID:SGTC_2141|Compound:5621638|FD-Score:3.37|P-value:4.54E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.47|P-value:3.16E-4 ID:SGTC_2464|Compound:5717415|FD-Score:3.35|P-value:4.89E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:-4.06|P-value:2.81E-5 ID:SGTC_2601|Compound:securinine|FD-Score:4.83|P-value:9.81E-7 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-3.36|P-value:4.19E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:5.66|P-value:1.21E-8 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:-3.17|P-value:8.17E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:4.30|P-value:1.14E-5 ID:SGTC_2838|Compound:9002687|FD-Score:3.28|P-value:6.18E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-4.17|P-value:1.74E-5 ID:SGTC_2919|Compound:7978188|FD-Score:3.88|P-value:6.63E-5 ID:SGTC_2940|Compound:9060638|FD-Score:-3.42|P-value:3.46E-4 ID:SGTC_3136|Compound:9095672|FD-Score:3.86|P-value:7.11E-5 ID:SGTC_3151|Compound:9099821|FD-Score:-4.06|P-value:2.80E-5 ID:SGTC_3155|Compound:9099265|FD-Score:-3.59|P-value:1.83E-4 ID:SGTC_3235|Compound:9132602|FD-Score:4.97|P-value:4.88E-7 ID:SGTC_48|Compound:0382-0018|FD-Score:-3.30|P-value:5.19E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:4.57|P-value:3.34E-6 ID:SGTC_104|Compound:0074-0074|FD-Score:3.43|P-value:3.65E-4 ID:SGTC_228|Compound:caffeine|FD-Score:-3.42|P-value:3.44E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:3.72|P-value:1.24E-4 ID:SGTC_371|Compound:0195-0006|FD-Score:4.44|P-value:6.21E-6 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:3.20|P-value:8.17E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:5.07|P-value:3.00E-7 ID:SGTC_670|Compound:4204-0025|FD-Score:3.41|P-value:4.02E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:4.44|P-value:6.07E-6 ID:SGTC_942|Compound:3253-1115|FD-Score:3.34|P-value:5.08E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:-3.76|P-value:9.38E-5 ID:SGTC_1182|Compound:1222-0449|FD-Score:3.21|P-value:8.08E-4 ID:SGTC_1281|Compound:0884-0031|FD-Score:3.42|P-value:3.83E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.33|P-value:5.23E-4 ID:SGTC_1620|Compound:st003704|FD-Score:3.65|P-value:1.61E-4 ID:SGTC_1663|Compound:st014140|FD-Score:-3.96|P-value:4.21E-5 ID:SGTC_1721|Compound:st082152|FD-Score:-3.25|P-value:6.22E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:3.30|P-value:5.93E-4 ID:SGTC_1916|Compound:st067606|FD-Score:-3.97|P-value:4.10E-5 ID:SGTC_1970|Compound:st070122|FD-Score:3.27|P-value:6.45E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.53|P-value:2.56E-4 ID:SGTC_2047|Compound:4141938|FD-Score:3.33|P-value:5.26E-4 ID:SGTC_2123|Compound:5259487|FD-Score:3.51|P-value:2.72E-4 ID:SGTC_2141|Compound:5621638|FD-Score:3.37|P-value:4.54E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.47|P-value:3.16E-4 ID:SGTC_2464|Compound:5717415|FD-Score:3.35|P-value:4.89E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:-4.06|P-value:2.81E-5 ID:SGTC_2601|Compound:securinine|FD-Score:4.83|P-value:9.81E-7 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-3.36|P-value:4.19E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:5.66|P-value:1.21E-8 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:-3.17|P-value:8.17E-4 ID:SGTC_2689|Compound:tacrolimus|FD-Score:4.30|P-value:1.14E-5 ID:SGTC_2838|Compound:9002687|FD-Score:3.28|P-value:6.18E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-4.17|P-value:1.74E-5 ID:SGTC_2919|Compound:7978188|FD-Score:3.88|P-value:6.63E-5 ID:SGTC_2940|Compound:9060638|FD-Score:-3.42|P-value:3.46E-4 ID:SGTC_3136|Compound:9095672|FD-Score:3.86|P-value:7.11E-5 ID:SGTC_3151|Compound:9099821|FD-Score:-4.06|P-value:2.80E-5 ID:SGTC_3155|Compound:9099265|FD-Score:-3.59|P-value:1.83E-4 ID:SGTC_3235|Compound:9132602|FD-Score:4.97|P-value:4.88E-7

Top fitness defect scores for YLR248W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2632 3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone 5.66 100.0 µM 1.21E-8 5 7.58E-9
2 SGTC_621 3584-0052 5.07 32.1 µM 3.00E-7 13 2.03E-7
3 SGTC_3235 9132602 4.97 49.5 µM 4.88E-7 9 3.36E-7
4 SGTC_2601 securinine 4.83 100.0 µM 9.81E-7 14 6.86E-7
5 SGTC_65 0798-0905 4.57 35.0 µM 3.34E-6 calcium & mitochondrial duress 63 2.41E-6
6 SGTC_753 1322-0200 4.44 289.0 µM 6.07E-6 9 4.46E-6
7 SGTC_371 0195-0006 4.44 7.4 µM 6.21E-6 12 4.56E-6
8 SGTC_2689 tacrolimus 4.30 100.0 µM 1.14E-5 35 8.49E-6
9 SGTC_2919 7978188 3.88 46.4 µM 6.63E-5 19 5.18E-5
10 SGTC_3136 9095672 3.86 49.5 µM 7.11E-5 26 5.56E-5
11 SGTC_365 0121-0052 3.72 13.6 µM 1.24E-4 mitochondrial response to ROS 65 9.83E-5
12 SGTC_1620 st003704 3.65 16.5 µM 1.61E-4 24 1.29E-4
13 SGTC_1998 st075797 3.53 76.8 µM 2.56E-4 mitochondrial response to ROS 26 2.07E-4
14 SGTC_2123 5259487 3.51 200.0 µM 2.72E-4 27 2.21E-4
15 SGTC_2336 9001819 3.47 198.7 µM 3.16E-4 heme biosynthesis & mitochondrial translocase 74 2.57E-4
16 SGTC_104 0074-0074 3.43 591.0 µM 3.65E-4 13 2.98E-4
17 SGTC_1281 0884-0031 3.42 11.8 µM 3.83E-4 fatty acid desaturase (OLE1) 40 3.13E-4
18 SGTC_670 4204-0025 3.41 119.0 µM 4.02E-4 copper-dependent oxidative stress 47 3.29E-4
19 SGTC_2141 5621638 3.37 7.7 µM 4.54E-4 tubulin folding & SWR complex 43 3.73E-4
20 SGTC_2464 5717415 3.35 7.7 µM 4.89E-4 33 4.03E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.109 2.22E-10 YJL150W_d YJL150W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.106 7.52E-10 YGR212W SLI1 hom N-acetyltransferase, confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin
0.104 1.78E-9 YMR296C LCB1 het Component of serine palmitoyltransferase, responsible along with Lcb2p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
0.095 3.20E-8 YJR099W YUH1 hom Ubiquitin C-terminal hydrolase that cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p
0.095 3.24E-8 YDR338C_p YDR338C_p hom Putative protein of unknown function, member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily
0.091 1.12E-7 YBR139W YBR139W hom Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner
0.088 3.15E-7 YJR016C ILV3 het Dihydroxyacid dehydratase, catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids
0.088 3.72E-7 YBR169C SSE2 hom Member of the heat shock protein 70 (HSP70) family; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication
0.087 3.99E-7 YGR213C RTA1 hom Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein
0.084 1.03E-6 YNL296W_d YNL296W_d hom Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog
0.083 1.53E-6 YMR114C YMR114C hom Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene
0.081 2.54E-6 YPL027W SMA1 hom Protein of unknown function involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p
0.080 3.73E-6 YJL038C LOH1 hom Protein of unknown function with proposed roles in maintenance of genome integrity and also in spore wall assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to IRC1
0.078 5.70E-6 YMR118C_p SHH3_p hom Putative mitochondrial inner membrane protein of unknown function; although similar to Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase
0.078 6.52E-6 YMR119W-A_d YMR119W-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data