HIPHOP chemogenomics database
YLR249W / YEF3 
Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
Zygosity: Heterozygous strain
fixedexpanded
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Top fitness defect scores for YLR249W deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Cofit Genes
Download Cofitness data (tab-delimited text) (excel)| Correlation | pval | ORF | Gene | Zygosity | Description |
|---|---|---|---|---|---|
| 0.154 | 2.26E-19 | YPL242C | IQG1 | het | Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress |
| 0.142 | 1.13E-16 | YKL024C | URA6 | het | Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP) |
| 0.129 | 5.39E-14 | YPR104C | FHL1 | het | Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants |
| 0.129 | 7.54E-14 | YOR159C | SME1 | het | Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E |
| 0.124 | 5.97E-13 | YER012W | PRE1 | het | Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle |
| 0.120 | 3.07E-12 | YER165W | PAB1 | het | Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G |
| 0.110 | 1.87E-10 | YLR117C | CLF1 | het | Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins |
| 0.107 | 5.32E-10 | YGR098C | ESP1 | het | Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress |
| 0.106 | 7.99E-10 | YBL004W | UTP20 | het | Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA |
| 0.105 | 9.24E-10 | YMR013W-A_d | YMR013W-A_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73 |
| 0.104 | 1.40E-9 | YMR093W | UTP15 | het | Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA |
| 0.104 | 1.78E-9 | YNR016C | ACC1 | het | Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids; |
| 0.102 | 2.92E-9 | YJL081C | ARP4 | het | Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes |
| 0.100 | 6.34E-9 | YCL017C | NFS1 | het | Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria |
| 0.099 | 7.78E-9 | YMR268C | PRP24 | het | Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling |