YLR249W / YEF3

Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex; contains two ABC cassettes; binds and hydrolyzes ATP; YEF3 has a paralog, HEF3, that arose from the whole genome duplication

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR249W / YEF3

Click on Significant Values for Screen Details ID:SGTC_68|Compound:0986-0284|FD-Score:3.49|P-value:1.51E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:4.74|P-value:4.27E-7 ID:SGTC_141|Compound:0443-0274|FD-Score:-6.99|P-value:1.31E-13 ID:SGTC_152|Compound:4469-0003|FD-Score:5.46|P-value:7.04E-9 ID:SGTC_163|Compound:1327-0036|FD-Score:3.90|P-value:2.65E-5 ID:SGTC_264|Compound:promethazine|FD-Score:3.12|P-value:6.27E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-3.83|P-value:2.90E-5 ID:SGTC_609|Compound:0986-0026|FD-Score:3.46|P-value:1.72E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:3.79|P-value:4.21E-5 ID:SGTC_673|Compound:1082-0474|FD-Score:4.17|P-value:7.70E-6 ID:SGTC_871|Compound:0574-0004|FD-Score:4.25|P-value:5.27E-6 ID:SGTC_913|Compound:3487-1245|FD-Score:4.18|P-value:7.19E-6 ID:SGTC_950|Compound:1165-0508|FD-Score:-3.38|P-value:1.92E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:3.10|P-value:6.62E-4 ID:SGTC_1085|Compound:doxorubicin|FD-Score:3.51|P-value:1.42E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:5.58|P-value:3.40E-9 ID:SGTC_1162|Compound:k048-0130|FD-Score:6.77|P-value:1.03E-12 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.41|P-value:2.06E-4 ID:SGTC_1421|Compound:4029-0249|FD-Score:3.13|P-value:5.94E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.00|P-value:9.71E-4 ID:SGTC_1482|Compound:3346-2049|FD-Score:4.30|P-value:4.13E-6 ID:SGTC_1505|Compound:k297-0037|FD-Score:3.01|P-value:9.24E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:3.69|P-value:6.70E-5 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:3.23|P-value:4.12E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.57|P-value:3.77E-9 ID:SGTC_1704|Compound:st028805|FD-Score:3.20|P-value:4.67E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-4.34|P-value:2.74E-6 ID:SGTC_1786|Compound:berberine|FD-Score:-3.63|P-value:6.98E-5 ID:SGTC_1846|Compound:st056244|FD-Score:3.16|P-value:5.46E-4 ID:SGTC_1854|Compound:st056233|FD-Score:-3.51|P-value:1.17E-4 ID:SGTC_1855|Compound:st056195|FD-Score:-3.05|P-value:6.70E-4 ID:SGTC_1942|Compound:st076330|FD-Score:-3.75|P-value:4.15E-5 ID:SGTC_1944|Compound:st076371|FD-Score:-3.42|P-value:1.64E-4 ID:SGTC_2041|Compound:5107442|FD-Score:3.39|P-value:2.25E-4 ID:SGTC_2049|Compound:5262831|FD-Score:-3.87|P-value:2.50E-5 ID:SGTC_2059|Compound:5238311|FD-Score:-3.68|P-value:5.59E-5 ID:SGTC_2060|Compound:5238662|FD-Score:-4.95|P-value:1.11E-7 ID:SGTC_2063|Compound:5246145|FD-Score:5.99|P-value:2.43E-10 ID:SGTC_2066|Compound:5222451|FD-Score:-4.99|P-value:8.86E-8 ID:SGTC_2068|Compound:5226020|FD-Score:3.27|P-value:3.55E-4 ID:SGTC_2072|Compound:5231554|FD-Score:-3.43|P-value:1.61E-4 ID:SGTC_2075|Compound:5212009|FD-Score:4.17|P-value:7.58E-6 ID:SGTC_2117|Compound:5521686|FD-Score:2.99|P-value:9.84E-4 ID:SGTC_2160|Compound:5660466|FD-Score:-3.19|P-value:4.09E-4 ID:SGTC_2248|Compound:tofa|FD-Score:3.47|P-value:1.61E-4 ID:SGTC_2318|Compound:6029776|FD-Score:3.03|P-value:8.49E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.85|P-value:3.31E-5 ID:SGTC_2480|Compound:5767542|FD-Score:-2.94|P-value:9.99E-4 ID:SGTC_2686|Compound:alizarin|FD-Score:3.71|P-value:6.05E-5 ID:SGTC_2695|Compound:st077686|FD-Score:-4.58|P-value:8.04E-7 ID:SGTC_2714|Compound:st079313|FD-Score:3.59|P-value:1.02E-4 ID:SGTC_2912|Compound:7964298|FD-Score:3.24|P-value:3.95E-4 ID:SGTC_2976|Compound:9009005|FD-Score:4.75|P-value:4.25E-7 ID:SGTC_2989|Compound:9073649|FD-Score:-3.54|P-value:1.01E-4 ID:SGTC_3137|Compound:9097352|FD-Score:4.67|P-value:6.45E-7 ID:SGTC_3192|Compound:9110362|FD-Score:-3.49|P-value:1.25E-4 ID:SGTC_68|Compound:0986-0284|FD-Score:3.49|P-value:1.51E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:4.74|P-value:4.27E-7 ID:SGTC_141|Compound:0443-0274|FD-Score:-6.99|P-value:1.31E-13 ID:SGTC_152|Compound:4469-0003|FD-Score:5.46|P-value:7.04E-9 ID:SGTC_163|Compound:1327-0036|FD-Score:3.90|P-value:2.65E-5 ID:SGTC_264|Compound:promethazine|FD-Score:3.12|P-value:6.27E-4 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:-3.83|P-value:2.90E-5 ID:SGTC_609|Compound:0986-0026|FD-Score:3.46|P-value:1.72E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:3.79|P-value:4.21E-5 ID:SGTC_673|Compound:1082-0474|FD-Score:4.17|P-value:7.70E-6 ID:SGTC_871|Compound:0574-0004|FD-Score:4.25|P-value:5.27E-6 ID:SGTC_913|Compound:3487-1245|FD-Score:4.18|P-value:7.19E-6 ID:SGTC_950|Compound:1165-0508|FD-Score:-3.38|P-value:1.92E-4 ID:SGTC_1019|Compound:4262-0212|FD-Score:3.10|P-value:6.62E-4 ID:SGTC_1085|Compound:doxorubicin|FD-Score:3.51|P-value:1.42E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:5.58|P-value:3.40E-9 ID:SGTC_1162|Compound:k048-0130|FD-Score:6.77|P-value:1.03E-12 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.41|P-value:2.06E-4 ID:SGTC_1421|Compound:4029-0249|FD-Score:3.13|P-value:5.94E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.00|P-value:9.71E-4 ID:SGTC_1482|Compound:3346-2049|FD-Score:4.30|P-value:4.13E-6 ID:SGTC_1505|Compound:k297-0037|FD-Score:3.01|P-value:9.24E-4 ID:SGTC_1514|Compound:1189-1591|FD-Score:3.69|P-value:6.70E-5 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:3.23|P-value:4.12E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:5.57|P-value:3.77E-9 ID:SGTC_1704|Compound:st028805|FD-Score:3.20|P-value:4.67E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-4.34|P-value:2.74E-6 ID:SGTC_1786|Compound:berberine|FD-Score:-3.63|P-value:6.98E-5 ID:SGTC_1846|Compound:st056244|FD-Score:3.16|P-value:5.46E-4 ID:SGTC_1854|Compound:st056233|FD-Score:-3.51|P-value:1.17E-4 ID:SGTC_1855|Compound:st056195|FD-Score:-3.05|P-value:6.70E-4 ID:SGTC_1942|Compound:st076330|FD-Score:-3.75|P-value:4.15E-5 ID:SGTC_1944|Compound:st076371|FD-Score:-3.42|P-value:1.64E-4 ID:SGTC_2041|Compound:5107442|FD-Score:3.39|P-value:2.25E-4 ID:SGTC_2049|Compound:5262831|FD-Score:-3.87|P-value:2.50E-5 ID:SGTC_2059|Compound:5238311|FD-Score:-3.68|P-value:5.59E-5 ID:SGTC_2060|Compound:5238662|FD-Score:-4.95|P-value:1.11E-7 ID:SGTC_2063|Compound:5246145|FD-Score:5.99|P-value:2.43E-10 ID:SGTC_2066|Compound:5222451|FD-Score:-4.99|P-value:8.86E-8 ID:SGTC_2068|Compound:5226020|FD-Score:3.27|P-value:3.55E-4 ID:SGTC_2072|Compound:5231554|FD-Score:-3.43|P-value:1.61E-4 ID:SGTC_2075|Compound:5212009|FD-Score:4.17|P-value:7.58E-6 ID:SGTC_2117|Compound:5521686|FD-Score:2.99|P-value:9.84E-4 ID:SGTC_2160|Compound:5660466|FD-Score:-3.19|P-value:4.09E-4 ID:SGTC_2248|Compound:tofa|FD-Score:3.47|P-value:1.61E-4 ID:SGTC_2318|Compound:6029776|FD-Score:3.03|P-value:8.49E-4 ID:SGTC_2396|Compound:5342950|FD-Score:3.85|P-value:3.31E-5 ID:SGTC_2480|Compound:5767542|FD-Score:-2.94|P-value:9.99E-4 ID:SGTC_2686|Compound:alizarin|FD-Score:3.71|P-value:6.05E-5 ID:SGTC_2695|Compound:st077686|FD-Score:-4.58|P-value:8.04E-7 ID:SGTC_2714|Compound:st079313|FD-Score:3.59|P-value:1.02E-4 ID:SGTC_2912|Compound:7964298|FD-Score:3.24|P-value:3.95E-4 ID:SGTC_2976|Compound:9009005|FD-Score:4.75|P-value:4.25E-7 ID:SGTC_2989|Compound:9073649|FD-Score:-3.54|P-value:1.01E-4 ID:SGTC_3137|Compound:9097352|FD-Score:4.67|P-value:6.45E-7 ID:SGTC_3192|Compound:9110362|FD-Score:-3.49|P-value:1.25E-4

Top fitness defect scores for YLR249W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1162 k048-0130 6.77 20.1 µM 1.03E-12 Golgi 4 6.56E-12 0.07
2 SGTC_2063 5246145 5.99 200.0 µM 2.43E-10 2 1.03E-9 0.94
3 SGTC_1133 3473-0247 5.58 50.0 µM 3.40E-9 RSC complex & mRNA processing 27 1.18E-8 0.03
4 SGTC_1597 7-benzyloxygramine 5.57 47.5 µM 3.77E-9 calcium & mitochondrial duress 38 1.30E-8 0.04
5 SGTC_152 4469-0003 5.46 551.2 µM 7.04E-9 5 2.32E-8 0.55
6 SGTC_2976 9009005 4.75 19.5 µM 4.25E-7 3 1.04E-6 0.65
7 SGTC_122 0236-0016 4.74 402.4 µM 4.27E-7 RNA processing & uracil transport 10 1.04E-6 0.17
8 SGTC_3137 9097352 4.67 49.5 µM 6.45E-7 1 1.53E-6 0.28
9 SGTC_1482 3346-2049 4.30 8.5 µM 4.13E-6 6 8.55E-6 0.59
10 SGTC_871 0574-0004 4.25 149.0 µM 5.27E-6 25 1.07E-5 0.09
11 SGTC_913 3487-1245 4.18 167.0 µM 7.19E-6 11 1.43E-5 0.01
12 SGTC_2075 5212009 4.17 200.0 µM 7.58E-6 6 1.50E-5 0.41
13 SGTC_673 1082-0474 4.17 110.0 µM 7.70E-6 RSC complex & mRNA processing 50 1.53E-5 0.08
14 SGTC_163 1327-0036 3.90 7.5 µM 2.65E-5 47 4.81E-5 0.08
15 SGTC_2396 5342950 3.85 42.8 µM 3.31E-5 RSC complex & mRNA processing 38 5.91E-5 0.12
16 SGTC_633 0437-0093 3.79 97.7 µM 4.21E-5 DNA intercalators 11 7.39E-5 0.04
17 SGTC_2686 alizarin 3.71 95.4 µM 6.05E-5 RPP1 & pyrimidine depletion 9 1.04E-4 0.01
18 SGTC_1514 1189-1591 3.69 158.0 µM 6.70E-5 7 1.14E-4 0.01
19 SGTC_2714 st079313 3.59 56.9 µM 1.02E-4 9 1.68E-4 0.05
20 SGTC_1085 doxorubicin 3.51 7.7 µM 1.42E-4 anthracycline transcription coupled DNA repair 11 2.28E-4 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.154 2.26E-19 YPL242C IQG1 het Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.142 1.13E-16 YKL024C URA6 het Uridylate kinase, catalyzes the seventh enzymatic step in the de novo biosynthesis of pyrimidines, converting uridine monophosphate (UMP) into uridine-5'-diphosphate (UDP)
0.129 5.39E-14 YPR104C FHL1 het Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
0.129 7.54E-14 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.124 5.97E-13 YER012W PRE1 het Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
0.120 3.07E-12 YER165W PAB1 het Poly(A) binding protein, part of the 3'-end RNA-processing complex, mediates interactions between the 5' cap structure and the 3' mRNA poly(A) tail, involved in control of poly(A) tail length, interacts with translation factor eIF-4G
0.110 1.87E-10 YLR117C CLF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
0.107 5.32E-10 YGR098C ESP1 het Separase, a caspase-like cysteine protease; promotes sister chromatid separation by mediating dissociation of the cohesin Scc1p from chromatin; inhibits protein phosphatase 2A-Cdc55p to promote mitotic exit; inhibited by Pds1p; relative distribution to the nucleus increases upon DNA replication stress
0.106 7.99E-10 YBL004W UTP20 het Component of the small-subunit (SSU) processome, which is involved in the biogenesis of the 18S rRNA
0.105 9.24E-10 YMR013W-A_d YMR013W-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73
0.104 1.40E-9 YMR093W UTP15 het Nucleolar protein, component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
0.104 1.78E-9 YNR016C ACC1 het Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
0.102 2.92E-9 YJL081C ARP4 het Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.100 6.34E-9 YCL017C NFS1 het Cysteine desulfurase involved in iron-sulfur cluster (Fe/S) biogenesis and in thio-modification of mitochondrial and cytoplasmic tRNAs; essential protein located predominantly in mitochondria
0.099 7.78E-9 YMR268C PRP24 het Splicing factor that reanneals U4 and U6 snRNPs during spliceosome recycling