HIPHOP chemogenomics database

YLR270W / DCS1

Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; interacts with neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain

fixedexpanded
Profile for YLR270W / DCS1

Click on Significant Values for Screen Details

Top fitness defect scores for YLR270W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_236	1509-0011	12.40	5.3 µM	8.20E-33		141	8.47E-36
2	SGTC_1742	st038235	7.19	28.9 µM	3.38E-12	mitochondrial stress	25	3.13E-13
3	SGTC_63	0099-0242	6.94	26.7 µM	1.81E-11	mitochondrial stress	18	1.96E-12
4	SGTC_2250	alverine citrate	6.72	130.0 µM	7.46E-11		37	9.28E-12
5	SGTC_979	1426-2828	6.18	121.0 µM	1.86E-9		3	3.14E-10
6	SGTC_2326	6164455	5.87	177.6 µM	1.09E-8	mitochondrial processes	3	2.17E-9
7	SGTC_3193	9111931	5.71	49.5 µM	2.55E-8		3	5.51E-9
8	SGTC_1722	st034304	5.64	3.6 µM	3.78E-8	mitochondrial processes	9	8.46E-9
9	SGTC_977	1348-1378	5.33	135.0 µM	1.86E-7		6	4.82E-8
10	SGTC_310	1273-0058	5.29	1.1 µM	2.31E-7	mitochondrial stress	169	6.14E-8
11	SGTC_68	0986-0284	4.97	82.8 µM	1.11E-6		33	3.42E-7
12	SGTC_2730	imipramine	4.93	71.3 µM	1.34E-6		4	4.19E-7
13	SGTC_1402	3909-7673	4.82	35.9 µM	2.14E-6		10	7.02E-7
14	SGTC_2296	7971656	4.81	126.5 µM	2.26E-6		24	7.42E-7
15	SGTC_2232	chlorophetanol	4.75	41.5 µM	3.03E-6	TRP & mitochondrial translation	88	1.02E-6
16	SGTC_717	1532-0246	4.51	73.2 µM	8.65E-6	Golgi	18	3.23E-6
17	SGTC_1719	st032227	4.35	49.2 µM	1.69E-5		15	6.73E-6
18	SGTC_1623	st003709	4.32	20.3 µM	1.92E-5	calcium & mitochondrial duress	54	7.71E-6
19	SGTC_596	1155-0152	4.22	116.0 µM	2.98E-5	TRP & mitochondrial translation	31	1.25E-5
20	SGTC_432	NS 1619	4.13	61.3 µM	4.13E-5		24	1.78E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.425	1.52E-147	YMR228W	MTF1	hom	Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.408	6.31E-134	YMR287C	DSS1	hom	3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.402	7.46E-131	YDR462W	MRPL28	hom	Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
0.399	3.68E-128	YDR194C	MSS116	hom	DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing
0.397	7.05E-127	YER050C	RSM18	hom	Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein
0.391	6.67E-123	YMR193W	MRPL24	hom	Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
0.385	5.04E-119	YDR114C_p	YDR114C_p	hom	Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium
0.379	4.47E-115	YPR100W	MRPL51	hom	Mitochondrial ribosomal protein of the large subunit
0.374	3.14E-112	YGR171C	MSM1	hom	Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p
0.371	5.58E-110	YGR215W	RSM27	hom	Mitochondrial ribosomal protein of the small subunit
0.371	6.51E-110	YPL040C	ISM1	hom	Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
0.366	5.08E-107	YGL064C	MRH4	hom	Mitochondrial ATP-dependent RNA helicase of the DEAD-box family, plays an essential role in mitochondrial function
0.364	6.70E-106	YDR296W	MHR1	hom	Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress
0.362	1.69E-104	YER077C_p	YER077C_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport
0.359	1.87E-102	YPL183W-A	RTC6	hom	Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress