YLR274W / MCM5

Component of the hexameric MCM complex, which is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR274W / MCM5

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:5.70|P-value:9.76E-8 ID:SGTC_147|Compound:4466-0538|FD-Score:4.44|P-value:2.54E-5 ID:SGTC_148|Compound:3253-0744|FD-Score:5.12|P-value:1.47E-6 ID:SGTC_155|Compound:k072-0202|FD-Score:-5.73|P-value:6.29E-8 ID:SGTC_428|Compound:3448-4153|FD-Score:3.70|P-value:3.68E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.82|P-value:4.36E-6 ID:SGTC_925|Compound:2064-1272|FD-Score:3.83|P-value:2.37E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:3.67|P-value:4.12E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:-3.71|P-value:3.10E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:4.17|P-value:7.19E-5 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.99|P-value:1.38E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:-3.56|P-value:4.98E-4 ID:SGTC_1697|Compound:st025554|FD-Score:3.74|P-value:3.24E-4 ID:SGTC_1703|Compound:st026983|FD-Score:3.45|P-value:8.27E-4 ID:SGTC_1707|Compound:st032323|FD-Score:3.91|P-value:1.81E-4 ID:SGTC_1748|Compound:st037455|FD-Score:-3.53|P-value:5.57E-4 ID:SGTC_1834|Compound:st055981|FD-Score:3.51|P-value:6.96E-4 ID:SGTC_1885|Compound:st057546|FD-Score:3.50|P-value:7.05E-4 ID:SGTC_1897|Compound:st060201|FD-Score:4.26|P-value:5.12E-5 ID:SGTC_1981|Compound:st071826|FD-Score:3.50|P-value:7.09E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.42|P-value:9.23E-4 ID:SGTC_1999|Compound:st074831|FD-Score:-3.66|P-value:3.60E-4 ID:SGTC_2089|Compound:5281248|FD-Score:3.65|P-value:4.44E-4 ID:SGTC_2099|Compound:5377090|FD-Score:-3.86|P-value:1.79E-4 ID:SGTC_2137|Compound:5261020|FD-Score:4.66|P-value:1.03E-5 ID:SGTC_2150|Compound:5556613|FD-Score:4.96|P-value:3.00E-6 ID:SGTC_2156|Compound:5649887|FD-Score:3.66|P-value:4.30E-4 ID:SGTC_2171|Compound:5739187|FD-Score:-4.24|P-value:4.58E-5 ID:SGTC_2210|Compound:7115885|FD-Score:3.40|P-value:9.73E-4 ID:SGTC_2268|Compound:7933382|FD-Score:4.91|P-value:3.65E-6 ID:SGTC_2308|Compound:7584454|FD-Score:-4.45|P-value:2.04E-5 ID:SGTC_2314|Compound:7727897|FD-Score:3.82|P-value:2.50E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-3.95|P-value:1.33E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:-3.58|P-value:4.63E-4 ID:SGTC_2444|Compound:5485484|FD-Score:4.49|P-value:2.09E-5 ID:SGTC_2493|Compound:resveratrol|FD-Score:-3.83|P-value:1.99E-4 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:3.61|P-value:4.99E-4 ID:SGTC_2660|Compound:2',4'-dihydroxychalcone|FD-Score:3.68|P-value:4.01E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.05|P-value:1.09E-4 ID:SGTC_2892|Compound:9047358|FD-Score:3.85|P-value:2.21E-4 ID:SGTC_2908|Compound:7930301|FD-Score:-3.91|P-value:1.51E-4 ID:SGTC_2978|Compound:9011784|FD-Score:3.60|P-value:5.15E-4 ID:SGTC_2980|Compound:9014342|FD-Score:3.78|P-value:2.81E-4 ID:SGTC_2989|Compound:9073649|FD-Score:3.85|P-value:2.26E-4 ID:SGTC_3194|Compound:9111903|FD-Score:3.83|P-value:2.38E-4 ID:SGTC_3286|Compound:9117135|FD-Score:5.45|P-value:3.23E-7 ID:SGTC_3290|Compound:9121031|FD-Score:-4.14|P-value:6.58E-5 ID:SGTC_3325|Compound:9138760|FD-Score:7.17|P-value:2.70E-11 ID:SGTC_3333|Compound:9141820|FD-Score:4.42|P-value:2.75E-5 ID:SGTC_3335|Compound:9143315|FD-Score:-4.34|P-value:3.10E-5 ID:SGTC_64|Compound:000l-0018|FD-Score:5.70|P-value:9.76E-8 ID:SGTC_147|Compound:4466-0538|FD-Score:4.44|P-value:2.54E-5 ID:SGTC_148|Compound:3253-0744|FD-Score:5.12|P-value:1.47E-6 ID:SGTC_155|Compound:k072-0202|FD-Score:-5.73|P-value:6.29E-8 ID:SGTC_428|Compound:3448-4153|FD-Score:3.70|P-value:3.68E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.82|P-value:4.36E-6 ID:SGTC_925|Compound:2064-1272|FD-Score:3.83|P-value:2.37E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:3.67|P-value:4.12E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:-3.71|P-value:3.10E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:4.17|P-value:7.19E-5 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.99|P-value:1.38E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:-3.56|P-value:4.98E-4 ID:SGTC_1697|Compound:st025554|FD-Score:3.74|P-value:3.24E-4 ID:SGTC_1703|Compound:st026983|FD-Score:3.45|P-value:8.27E-4 ID:SGTC_1707|Compound:st032323|FD-Score:3.91|P-value:1.81E-4 ID:SGTC_1748|Compound:st037455|FD-Score:-3.53|P-value:5.57E-4 ID:SGTC_1834|Compound:st055981|FD-Score:3.51|P-value:6.96E-4 ID:SGTC_1885|Compound:st057546|FD-Score:3.50|P-value:7.05E-4 ID:SGTC_1897|Compound:st060201|FD-Score:4.26|P-value:5.12E-5 ID:SGTC_1981|Compound:st071826|FD-Score:3.50|P-value:7.09E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.42|P-value:9.23E-4 ID:SGTC_1999|Compound:st074831|FD-Score:-3.66|P-value:3.60E-4 ID:SGTC_2089|Compound:5281248|FD-Score:3.65|P-value:4.44E-4 ID:SGTC_2099|Compound:5377090|FD-Score:-3.86|P-value:1.79E-4 ID:SGTC_2137|Compound:5261020|FD-Score:4.66|P-value:1.03E-5 ID:SGTC_2150|Compound:5556613|FD-Score:4.96|P-value:3.00E-6 ID:SGTC_2156|Compound:5649887|FD-Score:3.66|P-value:4.30E-4 ID:SGTC_2171|Compound:5739187|FD-Score:-4.24|P-value:4.58E-5 ID:SGTC_2210|Compound:7115885|FD-Score:3.40|P-value:9.73E-4 ID:SGTC_2268|Compound:7933382|FD-Score:4.91|P-value:3.65E-6 ID:SGTC_2308|Compound:7584454|FD-Score:-4.45|P-value:2.04E-5 ID:SGTC_2314|Compound:7727897|FD-Score:3.82|P-value:2.50E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-3.95|P-value:1.33E-4 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:-3.58|P-value:4.63E-4 ID:SGTC_2444|Compound:5485484|FD-Score:4.49|P-value:2.09E-5 ID:SGTC_2493|Compound:resveratrol|FD-Score:-3.83|P-value:1.99E-4 ID:SGTC_2496|Compound:diffractaic acid|FD-Score:3.61|P-value:4.99E-4 ID:SGTC_2660|Compound:2',4'-dihydroxychalcone|FD-Score:3.68|P-value:4.01E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.05|P-value:1.09E-4 ID:SGTC_2892|Compound:9047358|FD-Score:3.85|P-value:2.21E-4 ID:SGTC_2908|Compound:7930301|FD-Score:-3.91|P-value:1.51E-4 ID:SGTC_2978|Compound:9011784|FD-Score:3.60|P-value:5.15E-4 ID:SGTC_2980|Compound:9014342|FD-Score:3.78|P-value:2.81E-4 ID:SGTC_2989|Compound:9073649|FD-Score:3.85|P-value:2.26E-4 ID:SGTC_3194|Compound:9111903|FD-Score:3.83|P-value:2.38E-4 ID:SGTC_3286|Compound:9117135|FD-Score:5.45|P-value:3.23E-7 ID:SGTC_3290|Compound:9121031|FD-Score:-4.14|P-value:6.58E-5 ID:SGTC_3325|Compound:9138760|FD-Score:7.17|P-value:2.70E-11 ID:SGTC_3333|Compound:9141820|FD-Score:4.42|P-value:2.75E-5 ID:SGTC_3335|Compound:9143315|FD-Score:-4.34|P-value:3.10E-5

Top fitness defect scores for YLR274W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3325 9138760 7.17 55.2 µM 2.70E-11 Golgi 1 3.68E-13 2.62
2 SGTC_64 000l-0018 5.70 272.1 µM 9.76E-8 ubiquinone biosynthesis & proteasome 29 6.16E-9 0.22
3 SGTC_3286 9117135 5.45 37.2 µM 3.23E-7 3 2.55E-8 0.44
4 SGTC_148 3253-0744 5.12 288.0 µM 1.47E-6 2 1.54E-7 0.18
5 SGTC_2150 5556613 4.96 200.0 µM 3.00E-6 1 3.58E-7 0.51
6 SGTC_2268 7933382 4.91 153.8 µM 3.65E-6 6 4.52E-7 1.15
7 SGTC_2137 5261020 4.66 140.0 µM 1.03E-5 4 1.55E-6 0.03
8 SGTC_2444 5485484 4.49 36.4 µM 2.09E-5 4 3.58E-6 0.19
9 SGTC_147 4466-0538 4.44 29.2 µM 2.54E-5 ergosterol biosynthesis 5 4.49E-6 0.10
10 SGTC_3333 9141820 4.42 12.1 µM 2.75E-5 4 4.95E-6 0.58
11 SGTC_1897 st060201 4.26 15.0 µM 5.12E-5 mitochondrial processes 5 1.03E-5 0.49
12 SGTC_966 1300-0360 4.17 30.2 µM 7.19E-5 heme requiring 13 1.54E-5 0.01
13 SGTC_2778 tioconazole 4.05 65.1 nM 1.09E-4 ergosterol depletion effects on membrane 28 2.53E-5 0.00
14 SGTC_1125 tnp00248 3.99 1.3 µM 1.38E-4 9 3.33E-5 0.14
15 SGTC_1707 st032323 3.91 6.3 µM 1.81E-4 DNA damage response 30 4.60E-5 0.07
16 SGTC_2892 9047358 3.85 58.4 µM 2.21E-4 2 5.82E-5 0.18
17 SGTC_2989 9073649 3.85 71.4 µM 2.26E-4 8 5.97E-5 0.03
18 SGTC_925 2064-1272 3.83 59.6 µM 2.37E-4 6 6.33E-5 0.15
19 SGTC_3194 9111903 3.83 49.5 µM 2.38E-4 6 6.36E-5 0.50
20 SGTC_2314 7727897 3.82 200.0 µM 2.50E-4 3 6.73E-5 0.35

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.104 1.28E-9 YPL217C BMS1 het GTPase required for synthesis of 40S ribosomal subunits and for processing the 35S pre-rRNA at sites A0, A1, and A2; interacts with Rcl1p, which stimulates its GTPase and U3 snoRNA binding activities; has similarity to Tsr1p
0.103 2.38E-9 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.100 7.02E-9 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.095 3.71E-8 YBR053C_p YBR053C_p hom Putative protein of unknown function; induced by cell wall perturbation
0.092 9.52E-8 YLR135W SLX4 hom Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress
0.091 1.19E-7 YER094C PUP3 het Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
0.090 1.73E-7 YBR003W COQ1 hom Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
0.088 2.99E-7 YMR314W PRE5 het Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.085 9.40E-7 YJL144W YJL144W hom Cytoplasmic hydrophilin essential in dessication-rehydration process; expression induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress
0.082 1.80E-6 YHR197W RIX1 het Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; RIX1 is an essential gene
0.082 1.87E-6 YBR270C_p BIT2_p hom Subunit of TORC2 membrane-associated complex; involved in regulation of actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication
0.082 2.09E-6 YGR260W TNA1 hom High affinity nicotinic acid plasma membrane permease, responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA)
0.081 2.31E-6 YLR345W_p YLR345W_p hom Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene
0.081 2.95E-6 YHR058C MED6 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress
0.079 4.63E-6 YOR257W CDC31 het Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation