YLR277C / YSH1

Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR277C / YSH1

Click on Significant Values for Screen Details ID:SGTC_40|Compound:0327-0103|FD-Score:-3.33|P-value:2.21E-4 ID:SGTC_109|Compound:0132-0036|FD-Score:3.28|P-value:2.54E-4 ID:SGTC_201|Compound:4262-0159|FD-Score:3.34|P-value:2.02E-4 ID:SGTC_569|Compound:4130-2812|FD-Score:2.97|P-value:8.04E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:4.54|P-value:7.34E-7 ID:SGTC_692|Compound:k241-0027|FD-Score:2.94|P-value:8.94E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:3.61|P-value:6.36E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:3.10|P-value:5.07E-4 ID:SGTC_797|Compound:k781-0471|FD-Score:-2.95|P-value:9.21E-4 ID:SGTC_802|Compound:4112-3352|FD-Score:-4.28|P-value:3.18E-6 ID:SGTC_806|Compound:3966-4455|FD-Score:-2.97|P-value:8.78E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:2.93|P-value:9.31E-4 ID:SGTC_935|Compound:2996-0153|FD-Score:3.58|P-value:7.41E-5 ID:SGTC_1000|Compound:4048-0884|FD-Score:4.01|P-value:1.08E-5 ID:SGTC_1034|Compound:k072-0165|FD-Score:3.21|P-value:3.33E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.40|P-value:1.53E-4 ID:SGTC_1745|Compound:st081495|FD-Score:5.50|P-value:2.86E-9 ID:SGTC_1749|Compound:st037932|FD-Score:-4.02|P-value:1.12E-5 ID:SGTC_2002|Compound:4023503|FD-Score:3.14|P-value:4.25E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.00|P-value:1.14E-5 ID:SGTC_2139|Compound:5578692|FD-Score:3.51|P-value:9.69E-5 ID:SGTC_2158|Compound:5657123|FD-Score:3.75|P-value:3.50E-5 ID:SGTC_2161|Compound:5662504|FD-Score:-3.99|P-value:1.27E-5 ID:SGTC_2162|Compound:5663267|FD-Score:4.43|P-value:1.31E-6 ID:SGTC_2229|Compound:7354935|FD-Score:3.48|P-value:1.10E-4 ID:SGTC_2264|Compound:7948757|FD-Score:-4.23|P-value:3.94E-6 ID:SGTC_2288|Compound:7946289|FD-Score:2.96|P-value:8.47E-4 ID:SGTC_2436|Compound:5633522|FD-Score:3.40|P-value:1.56E-4 ID:SGTC_2441|Compound:5730946|FD-Score:3.50|P-value:1.02E-4 ID:SGTC_2443|Compound:5493264|FD-Score:3.59|P-value:7.02E-5 ID:SGTC_2457|Compound:5731572|FD-Score:3.87|P-value:2.06E-5 ID:SGTC_2467|Compound:5474540|FD-Score:3.14|P-value:4.31E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.34|P-value:2.14E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:3.48|P-value:1.13E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.02|P-value:7.32E-4 ID:SGTC_2720|Compound:fluspirilene|FD-Score:3.02|P-value:6.82E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:-3.13|P-value:4.85E-4 ID:SGTC_2805|Compound:7950783|FD-Score:-3.31|P-value:2.41E-4 ID:SGTC_2812|Compound:7970281|FD-Score:3.76|P-value:3.38E-5 ID:SGTC_2814|Compound:7977482|FD-Score:4.77|P-value:2.10E-7 ID:SGTC_2816|Compound:7982706|FD-Score:3.14|P-value:4.29E-4 ID:SGTC_2827|Compound:7993847|FD-Score:3.16|P-value:4.05E-4 ID:SGTC_2830|Compound:7992007|FD-Score:-3.47|P-value:1.27E-4 ID:SGTC_2988|Compound:9073640|FD-Score:3.30|P-value:2.34E-4 ID:SGTC_3284|Compound:9108171|FD-Score:3.00|P-value:7.16E-4 ID:SGTC_3292|Compound:9120825|FD-Score:4.02|P-value:1.03E-5 ID:SGTC_3295|Compound:9122362|FD-Score:4.09|P-value:7.26E-6 ID:SGTC_3327|Compound:9140648|FD-Score:3.67|P-value:4.90E-5 ID:SGTC_40|Compound:0327-0103|FD-Score:-3.33|P-value:2.21E-4 ID:SGTC_109|Compound:0132-0036|FD-Score:3.28|P-value:2.54E-4 ID:SGTC_201|Compound:4262-0159|FD-Score:3.34|P-value:2.02E-4 ID:SGTC_569|Compound:4130-2812|FD-Score:2.97|P-value:8.04E-4 ID:SGTC_621|Compound:3584-0052|FD-Score:4.54|P-value:7.34E-7 ID:SGTC_692|Compound:k241-0027|FD-Score:2.94|P-value:8.94E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:3.61|P-value:6.36E-5 ID:SGTC_778|Compound:3456-2434|FD-Score:3.10|P-value:5.07E-4 ID:SGTC_797|Compound:k781-0471|FD-Score:-2.95|P-value:9.21E-4 ID:SGTC_802|Compound:4112-3352|FD-Score:-4.28|P-value:3.18E-6 ID:SGTC_806|Compound:3966-4455|FD-Score:-2.97|P-value:8.78E-4 ID:SGTC_871|Compound:0574-0004|FD-Score:2.93|P-value:9.31E-4 ID:SGTC_935|Compound:2996-0153|FD-Score:3.58|P-value:7.41E-5 ID:SGTC_1000|Compound:4048-0884|FD-Score:4.01|P-value:1.08E-5 ID:SGTC_1034|Compound:k072-0165|FD-Score:3.21|P-value:3.33E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.40|P-value:1.53E-4 ID:SGTC_1745|Compound:st081495|FD-Score:5.50|P-value:2.86E-9 ID:SGTC_1749|Compound:st037932|FD-Score:-4.02|P-value:1.12E-5 ID:SGTC_2002|Compound:4023503|FD-Score:3.14|P-value:4.25E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.00|P-value:1.14E-5 ID:SGTC_2139|Compound:5578692|FD-Score:3.51|P-value:9.69E-5 ID:SGTC_2158|Compound:5657123|FD-Score:3.75|P-value:3.50E-5 ID:SGTC_2161|Compound:5662504|FD-Score:-3.99|P-value:1.27E-5 ID:SGTC_2162|Compound:5663267|FD-Score:4.43|P-value:1.31E-6 ID:SGTC_2229|Compound:7354935|FD-Score:3.48|P-value:1.10E-4 ID:SGTC_2264|Compound:7948757|FD-Score:-4.23|P-value:3.94E-6 ID:SGTC_2288|Compound:7946289|FD-Score:2.96|P-value:8.47E-4 ID:SGTC_2436|Compound:5633522|FD-Score:3.40|P-value:1.56E-4 ID:SGTC_2441|Compound:5730946|FD-Score:3.50|P-value:1.02E-4 ID:SGTC_2443|Compound:5493264|FD-Score:3.59|P-value:7.02E-5 ID:SGTC_2457|Compound:5731572|FD-Score:3.87|P-value:2.06E-5 ID:SGTC_2467|Compound:5474540|FD-Score:3.14|P-value:4.31E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.34|P-value:2.14E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:3.48|P-value:1.13E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.02|P-value:7.32E-4 ID:SGTC_2720|Compound:fluspirilene|FD-Score:3.02|P-value:6.82E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:-3.13|P-value:4.85E-4 ID:SGTC_2805|Compound:7950783|FD-Score:-3.31|P-value:2.41E-4 ID:SGTC_2812|Compound:7970281|FD-Score:3.76|P-value:3.38E-5 ID:SGTC_2814|Compound:7977482|FD-Score:4.77|P-value:2.10E-7 ID:SGTC_2816|Compound:7982706|FD-Score:3.14|P-value:4.29E-4 ID:SGTC_2827|Compound:7993847|FD-Score:3.16|P-value:4.05E-4 ID:SGTC_2830|Compound:7992007|FD-Score:-3.47|P-value:1.27E-4 ID:SGTC_2988|Compound:9073640|FD-Score:3.30|P-value:2.34E-4 ID:SGTC_3284|Compound:9108171|FD-Score:3.00|P-value:7.16E-4 ID:SGTC_3292|Compound:9120825|FD-Score:4.02|P-value:1.03E-5 ID:SGTC_3295|Compound:9122362|FD-Score:4.09|P-value:7.26E-6 ID:SGTC_3327|Compound:9140648|FD-Score:3.67|P-value:4.90E-5

Top fitness defect scores for YLR277C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1745 st081495 5.50 74.0 µM 2.86E-9 RNA processing & uracil transport 16 1.92E-8 0.44
2 SGTC_2814 7977482 4.77 43.0 µM 2.10E-7 6 9.01E-7 0.02
3 SGTC_621 3584-0052 4.54 32.1 µM 7.34E-7 3 2.77E-6 0.86
4 SGTC_2162 5663267 4.43 200.0 µM 1.31E-6 6 4.64E-6 0.07
5 SGTC_3295 9122362 4.09 53.6 µM 7.26E-6 6 2.16E-5 0.25
6 SGTC_3292 9120825 4.02 59.8 µM 1.03E-5 2 2.96E-5 0.28
7 SGTC_1000 4048-0884 4.01 8.7 µM 1.08E-5 copper-dependent oxidative stress 6 3.07E-5 0.26
8 SGTC_2021 4019055 4.00 173.0 µM 1.14E-5 endomembrane recycling 31 3.22E-5 0.02
9 SGTC_2457 5731572 3.87 11.7 µM 2.06E-5 1 5.49E-5 0.27
10 SGTC_2812 7970281 3.76 58.4 µM 3.38E-5 12 8.57E-5 0.15
11 SGTC_2158 5657123 3.75 3.6 µM 3.50E-5 15 8.83E-5 0.08
12 SGTC_3327 9140648 3.67 26.9 µM 4.90E-5 17 1.20E-4 0.01
13 SGTC_711 k292-0785 3.61 179.0 µM 6.36E-5 DNA intercalators 8 1.51E-4 0.10
14 SGTC_2443 5493264 3.59 126.7 µM 7.02E-5 10 1.65E-4 0.25
15 SGTC_935 2996-0153 3.58 430.0 µM 7.41E-5 PDR1 2 1.73E-4 0.20
16 SGTC_2139 5578692 3.51 56.5 µM 9.69E-5 superoxide 22 2.20E-4 0.18
17 SGTC_2441 5730946 3.50 39.1 µM 1.02E-4 5 2.30E-4 0.05
18 SGTC_2229 7354935 3.48 190.0 µM 1.10E-4 RSC complex & mRNA processing 24 2.47E-4 0.22
19 SGTC_2539 epi(13)torulosol 3.48 80.0 µM 1.13E-4 8 2.52E-4 0.09
20 SGTC_1284 0929-0043 3.40 16.0 µM 1.53E-4 6 3.32E-4 0.10

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.183 1.16E-26 YDR020C_p DAS2_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases
0.148 6.98E-18 YIL104C SHQ1 het Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones
0.132 1.75E-14 YLR097C HRT3 hom Putative SCF-ubiquitin ligase F-box protein, based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies
0.106 7.42E-10 YDR308C SRB7 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p
0.093 7.47E-8 YDR135C YCF1 hom Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR
0.090 1.70E-7 YPR186C PZF1 het Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
0.087 4.32E-7 YBR214W SDS24 hom Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication
0.086 5.76E-7 YKL112W ABF1 het DNA binding protein with possible chromatin-reorganizing activity involved in transcriptional activation, gene silencing, and DNA replication and repair
0.085 7.79E-7 YKR056W TRM2 hom tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair
0.080 3.38E-6 YDR289C RTT103 hom Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition
0.078 7.10E-6 YOL082W ATG19 hom Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles
0.077 8.74E-6 YGR013W SNU71 het Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart
0.076 9.43E-6 YGR009C SEC9 het t-SNARE protein important for fusion of secretory vesicles with the plasma membrane; similar to but not functionally redundant with Spo20p; SNAP-25 homolog
0.076 9.93E-6 YGR140W CBF2 het Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; relative distribution to the spindle pole body decreases upon DNA replication stress
0.074 1.88E-5 YOR060C SLD7 het Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria