HIPHOP chemogenomics database

YLR288C / MEC3

DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1

Zygosity: Homozygous strain

fixedexpanded
Profile for YLR288C / MEC3

Click on Significant Values for Screen Details

Top fitness defect scores for YLR288C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2494	18-aminoabieta-8,11,13-triene sulfate	7.73	3.7 µM	3.35E-18		3	5.30E-15
2	SGTC_6	0844-0013	6.25	19.8 µM	1.69E-12		11	2.10E-10
3	SGTC_2182	6325192	6.05	200.0 µM	8.07E-12	Golgi	3	7.41E-10
4	SGTC_2017	5132927	5.72	60.6 µM	9.46E-11		6	5.42E-9
5	SGTC_2501	sodium tetradecyl sulfate	4.26	13.4 µM	1.06E-6		26	1.02E-5
6	SGTC_1360	1598-0022	4.18	39.8 µM	1.61E-6	ERG2	24	1.44E-5
7	SGTC_2592	piperlongumine	4.04	83.3 µM	3.44E-6	TSC3-RPN4	36	2.67E-5
8	SGTC_2038	4100038	3.86	31.0 µM	8.64E-6	plasma membrane duress	55	5.64E-5
9	SGTC_1673	st016611	3.77	27.3 µM	1.38E-5		50	8.26E-5
10	SGTC_2503	beta-caryophyllene alcohol	3.72	24.7 µM	1.71E-5		14	9.82E-5
11	SGTC_2493	resveratrol	3.62	26.1 µM	2.77E-5		13	1.45E-4
12	SGTC_669	0108-0021	3.52	26.2 µM	4.58E-5	fatty acid desaturase (OLE1)	111	2.19E-4
13	SGTC_2492	derrusnin	3.47	100.0 µM	5.65E-5		10	2.60E-4
14	SGTC_1889	st059080	3.39	5.2 µM	7.99E-5		40	3.45E-4
15	SGTC_1258	0671-0043	3.28	57.1 µM	1.34E-4		55	5.25E-4
16	SGTC_2680	butyl paraben	3.24	22.2 µM	1.55E-4	amide catabolism	25	5.92E-4
17	SGTC_1385	3091-4834	3.18	14.1 µM	2.02E-4	RPP1 & pyrimidine depletion	43	7.34E-4
18	SGTC_1350	1495-0454	3.10	146.0 µM	2.84E-4		67	9.69E-4
19	SGTC_2114	5491910	3.06	81.2 µM	3.37E-4		46	0.00112
20	SGTC_2055	5235184	3.06	200.0 µM	3.37E-4	ERG2	74	0.00112

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.265	3.68E-55	YER067C-A_d	YER067C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.233	1.11E-42	YGR032W	GSC2	hom	Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; has similarity to an alternate catalytic subunit, Fks1p (Gsc1p)
0.231	7.41E-42	YML053C_p	YML053C_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene
0.222	8.60E-39	YGL017W	ATE1	hom	Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
0.208	4.33E-34	YDL110C	TMA17	hom	Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.194	6.69E-30	YDL049C	KNH1	hom	Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.193	2.42E-29	YER060W-A	FCY22	hom	Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function
0.190	1.04E-28	YNL242W	ATG2	hom	Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.190	1.28E-28	YKL205W	LOS1	hom	Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm
0.190	1.63E-28	YEL049W	PAU2	hom	Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
0.187	1.01E-27	YOR300W_d	YOR300W_d	hom	Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7
0.186	1.94E-27	YJR056C	YJR056C	hom	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
0.184	8.07E-27	YLR026C	SED5	het	cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
0.182	1.90E-26	YDR051C	DET1	hom	Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
0.177	4.54E-25	YKL131W_d	YKL131W_d	hom	Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data