YLR289W / GUF1

Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR289W / GUF1

Click on Significant Values for Screen Details ID:SGTC_26|Compound:0302-0163|FD-Score:-4.82|P-value:3.29E-6 ID:SGTC_35|Compound:0133-0054|FD-Score:-3.55|P-value:4.62E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:3.96|P-value:1.00E-4 ID:SGTC_74|Compound:000s-0487|FD-Score:5.75|P-value:3.47E-8 ID:SGTC_103|Compound:0844-0104|FD-Score:3.62|P-value:3.38E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:5.26|P-value:3.98E-7 ID:SGTC_251|Compound:melphalan|FD-Score:4.78|P-value:3.69E-6 ID:SGTC_599|Compound:1935-0052|FD-Score:3.45|P-value:6.03E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:3.32|P-value:9.19E-4 ID:SGTC_606|Compound:k015-0008|FD-Score:3.40|P-value:6.99E-4 ID:SGTC_671|Compound:0560-0003|FD-Score:3.71|P-value:2.43E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:-4.77|P-value:4.24E-6 ID:SGTC_705|Compound:1124-0212|FD-Score:5.43|P-value:1.73E-7 ID:SGTC_831|Compound:k061-1017|FD-Score:3.45|P-value:5.94E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:4.71|P-value:4.95E-6 ID:SGTC_873|Compound:0591-4978|FD-Score:4.33|P-value:2.37E-5 ID:SGTC_874|Compound:0599-0792|FD-Score:6.66|P-value:2.12E-10 ID:SGTC_896|Compound:0964-0007|FD-Score:4.10|P-value:5.88E-5 ID:SGTC_991|Compound:3996-0057|FD-Score:3.58|P-value:3.86E-4 ID:SGTC_1007|Compound:4101-0031|FD-Score:3.52|P-value:4.67E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.81|P-value:1.72E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:-3.45|P-value:6.42E-4 ID:SGTC_1148|Compound:3909-7663|FD-Score:4.31|P-value:2.59E-5 ID:SGTC_1265|Compound:0819-1001|FD-Score:-3.38|P-value:8.11E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.75|P-value:2.31E-4 ID:SGTC_1662|Compound:st014054|FD-Score:3.44|P-value:6.16E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.47|P-value:5.63E-4 ID:SGTC_1683|Compound:st010134|FD-Score:4.24|P-value:3.38E-5 ID:SGTC_1917|Compound:st066507|FD-Score:3.80|P-value:1.80E-4 ID:SGTC_1922|Compound:5578187|FD-Score:3.96|P-value:9.92E-5 ID:SGTC_1991|Compound:st073000|FD-Score:5.87|P-value:1.85E-8 ID:SGTC_2244|Compound:6635614|FD-Score:-3.88|P-value:1.45E-4 ID:SGTC_2255|Compound:7941087|FD-Score:3.36|P-value:7.90E-4 ID:SGTC_2256|Compound:7954252|FD-Score:-3.78|P-value:2.06E-4 ID:SGTC_2277|Compound:7947813|FD-Score:3.74|P-value:2.20E-4 ID:SGTC_2286|Compound:7945697|FD-Score:-4.39|P-value:2.07E-5 ID:SGTC_2320|Compound:7373003|FD-Score:4.21|P-value:3.83E-5 ID:SGTC_2393|Compound:9031399|FD-Score:4.28|P-value:2.95E-5 ID:SGTC_2397|Compound:5161689|FD-Score:4.26|P-value:3.12E-5 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.17|P-value:4.50E-5 ID:SGTC_2457|Compound:5731572|FD-Score:4.73|P-value:4.41E-6 ID:SGTC_2484|Compound:5809818|FD-Score:-4.41|P-value:1.92E-5 ID:SGTC_2486|Compound:5283202|FD-Score:3.57|P-value:3.93E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:4.66|P-value:6.18E-6 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.48|P-value:5.45E-4 ID:SGTC_2696|Compound:st077691|FD-Score:5.91|P-value:1.50E-8 ID:SGTC_2843|Compound:9000894|FD-Score:4.06|P-value:6.94E-5 ID:SGTC_2954|Compound:9074818|FD-Score:5.63|P-value:6.38E-8 ID:SGTC_3167|Compound:9103034|FD-Score:-3.57|P-value:4.24E-4 ID:SGTC_3174|Compound:9107204|FD-Score:5.01|P-value:1.31E-6 ID:SGTC_3233|Compound:9132340|FD-Score:3.64|P-value:3.18E-4 ID:SGTC_3295|Compound:9122362|FD-Score:3.76|P-value:2.07E-4 ID:SGTC_3311|Compound:9131146|FD-Score:-3.61|P-value:3.75E-4 ID:SGTC_3312|Compound:9133330|FD-Score:-3.72|P-value:2.58E-4 ID:SGTC_26|Compound:0302-0163|FD-Score:-4.82|P-value:3.29E-6 ID:SGTC_35|Compound:0133-0054|FD-Score:-3.55|P-value:4.62E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:3.96|P-value:1.00E-4 ID:SGTC_74|Compound:000s-0487|FD-Score:5.75|P-value:3.47E-8 ID:SGTC_103|Compound:0844-0104|FD-Score:3.62|P-value:3.38E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:5.26|P-value:3.98E-7 ID:SGTC_251|Compound:melphalan|FD-Score:4.78|P-value:3.69E-6 ID:SGTC_599|Compound:1935-0052|FD-Score:3.45|P-value:6.03E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:3.32|P-value:9.19E-4 ID:SGTC_606|Compound:k015-0008|FD-Score:3.40|P-value:6.99E-4 ID:SGTC_671|Compound:0560-0003|FD-Score:3.71|P-value:2.43E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:-4.77|P-value:4.24E-6 ID:SGTC_705|Compound:1124-0212|FD-Score:5.43|P-value:1.73E-7 ID:SGTC_831|Compound:k061-1017|FD-Score:3.45|P-value:5.94E-4 ID:SGTC_858|Compound:0438-0351|FD-Score:4.71|P-value:4.95E-6 ID:SGTC_873|Compound:0591-4978|FD-Score:4.33|P-value:2.37E-5 ID:SGTC_874|Compound:0599-0792|FD-Score:6.66|P-value:2.12E-10 ID:SGTC_896|Compound:0964-0007|FD-Score:4.10|P-value:5.88E-5 ID:SGTC_991|Compound:3996-0057|FD-Score:3.58|P-value:3.86E-4 ID:SGTC_1007|Compound:4101-0031|FD-Score:3.52|P-value:4.67E-4 ID:SGTC_1015|Compound:4130-4525|FD-Score:3.81|P-value:1.72E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:-3.45|P-value:6.42E-4 ID:SGTC_1148|Compound:3909-7663|FD-Score:4.31|P-value:2.59E-5 ID:SGTC_1265|Compound:0819-1001|FD-Score:-3.38|P-value:8.11E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.75|P-value:2.31E-4 ID:SGTC_1662|Compound:st014054|FD-Score:3.44|P-value:6.16E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.47|P-value:5.63E-4 ID:SGTC_1683|Compound:st010134|FD-Score:4.24|P-value:3.38E-5 ID:SGTC_1917|Compound:st066507|FD-Score:3.80|P-value:1.80E-4 ID:SGTC_1922|Compound:5578187|FD-Score:3.96|P-value:9.92E-5 ID:SGTC_1991|Compound:st073000|FD-Score:5.87|P-value:1.85E-8 ID:SGTC_2244|Compound:6635614|FD-Score:-3.88|P-value:1.45E-4 ID:SGTC_2255|Compound:7941087|FD-Score:3.36|P-value:7.90E-4 ID:SGTC_2256|Compound:7954252|FD-Score:-3.78|P-value:2.06E-4 ID:SGTC_2277|Compound:7947813|FD-Score:3.74|P-value:2.20E-4 ID:SGTC_2286|Compound:7945697|FD-Score:-4.39|P-value:2.07E-5 ID:SGTC_2320|Compound:7373003|FD-Score:4.21|P-value:3.83E-5 ID:SGTC_2393|Compound:9031399|FD-Score:4.28|P-value:2.95E-5 ID:SGTC_2397|Compound:5161689|FD-Score:4.26|P-value:3.12E-5 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.17|P-value:4.50E-5 ID:SGTC_2457|Compound:5731572|FD-Score:4.73|P-value:4.41E-6 ID:SGTC_2484|Compound:5809818|FD-Score:-4.41|P-value:1.92E-5 ID:SGTC_2486|Compound:5283202|FD-Score:3.57|P-value:3.93E-4 ID:SGTC_2583|Compound:asiatic acid|FD-Score:4.66|P-value:6.18E-6 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.48|P-value:5.45E-4 ID:SGTC_2696|Compound:st077691|FD-Score:5.91|P-value:1.50E-8 ID:SGTC_2843|Compound:9000894|FD-Score:4.06|P-value:6.94E-5 ID:SGTC_2954|Compound:9074818|FD-Score:5.63|P-value:6.38E-8 ID:SGTC_3167|Compound:9103034|FD-Score:-3.57|P-value:4.24E-4 ID:SGTC_3174|Compound:9107204|FD-Score:5.01|P-value:1.31E-6 ID:SGTC_3233|Compound:9132340|FD-Score:3.64|P-value:3.18E-4 ID:SGTC_3295|Compound:9122362|FD-Score:3.76|P-value:2.07E-4 ID:SGTC_3311|Compound:9131146|FD-Score:-3.61|P-value:3.75E-4 ID:SGTC_3312|Compound:9133330|FD-Score:-3.72|P-value:2.58E-4

Top fitness defect scores for YLR289W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_874 0599-0792 6.66 117.0 µM 2.12E-10 2 1.39E-11
2 SGTC_2696 st077691 5.91 74.3 µM 1.50E-8 6 1.75E-9
3 SGTC_1991 st073000 5.87 8.9 µM 1.85E-8 NEO1 38 2.21E-9
4 SGTC_74 000s-0487 5.75 160.3 µM 3.47E-8 7 4.54E-9
5 SGTC_2954 9074818 5.63 52.7 µM 6.38E-8 14 9.03E-9
6 SGTC_705 1124-0212 5.43 310.0 µM 1.73E-7 4 2.79E-8
7 SGTC_245 methotrexate 5.26 289.6 µM 3.98E-7 17 7.20E-8
8 SGTC_3174 9107204 5.01 49.5 µM 1.31E-6 ERG2 11 2.78E-7
9 SGTC_251 melphalan 4.78 456.5 µM 3.69E-6 DNA damage response 10 8.96E-7
10 SGTC_2457 5731572 4.73 11.7 µM 4.41E-6 9 1.10E-6
11 SGTC_858 0438-0351 4.71 2.8 µM 4.95E-6 copper-dependent oxidative stress 9 1.25E-6
12 SGTC_2583 asiatic acid 4.66 100.0 µM 6.18E-6 24 1.61E-6
13 SGTC_873 0591-4978 4.33 488.0 µM 2.37E-5 13 7.35E-6
14 SGTC_1148 3909-7663 4.31 27.7 µM 2.59E-5 9 8.12E-6
15 SGTC_2393 9031399 4.28 185.9 µM 2.95E-5 RNA processing & uracil transport 20 9.42E-6
16 SGTC_2397 5161689 4.26 200.0 µM 3.12E-5 24 1.00E-5
17 SGTC_1683 st010134 4.24 710.0 nM 3.38E-5 19 1.10E-5
18 SGTC_2320 7373003 4.21 200.0 µM 3.83E-5 22 1.27E-5
19 SGTC_2407 paclitaxel 4.17 400.0 µM 4.50E-5 28 1.52E-5
20 SGTC_896 0964-0007 4.10 44.5 µM 5.88E-5 14 2.06E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.107 5.13E-10 YDR196C CAB5 het Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies
0.101 4.50E-9 YGR154C GTO1 hom Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization
0.096 2.87E-8 YNL179C_d YNL179C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance
0.091 1.18E-7 YLL048C YBT1 hom Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
0.090 1.69E-7 YCL022C_d YCL022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W
0.089 2.22E-7 YLL041C SDH2 hom Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain
0.089 2.31E-7 YOL057W YOL057W hom Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers
0.088 3.62E-7 YNL055C POR1 hom Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication
0.085 9.41E-7 YLR215C CDC123 het Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein
0.081 2.44E-6 YKL077W_p YKL077W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.079 4.06E-6 YBR259W YBR259W hom Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress
0.078 5.40E-6 YLR180W SAM1 hom S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication
0.078 6.39E-6 YDR513W GRX2 hom Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication
0.077 9.00E-6 YGR256W GND2 hom 6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication
0.076 1.06E-5 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability