HIPHOP chemogenomics database

YLR289W / GUF1

Mitochondrial matrix GTPase that associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor

Zygosity: Homozygous strain

fixedexpanded
Profile for YLR289W / GUF1

Click on Significant Values for Screen Details

Top fitness defect scores for YLR289W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_874	0599-0792	6.66	117.0 µM	2.12E-10		2	1.39E-11
2	SGTC_2696	st077691	5.91	74.3 µM	1.50E-8		6	1.75E-9
3	SGTC_1991	st073000	5.87	8.9 µM	1.85E-8	NEO1	38	2.21E-9
4	SGTC_74	000s-0487	5.75	160.3 µM	3.47E-8		7	4.54E-9
5	SGTC_2954	9074818	5.63	52.7 µM	6.38E-8		14	9.03E-9
6	SGTC_705	1124-0212	5.43	310.0 µM	1.73E-7		4	2.79E-8
7	SGTC_245	methotrexate	5.26	289.6 µM	3.98E-7		17	7.20E-8
8	SGTC_3174	9107204	5.01	49.5 µM	1.31E-6	ERG2	11	2.78E-7
9	SGTC_251	melphalan	4.78	456.5 µM	3.69E-6	DNA damage response	10	8.96E-7
10	SGTC_2457	5731572	4.73	11.7 µM	4.41E-6		9	1.10E-6
11	SGTC_858	0438-0351	4.71	2.8 µM	4.95E-6	copper-dependent oxidative stress	9	1.25E-6
12	SGTC_2583	asiatic acid	4.66	100.0 µM	6.18E-6		24	1.61E-6
13	SGTC_873	0591-4978	4.33	488.0 µM	2.37E-5		13	7.35E-6
14	SGTC_1148	3909-7663	4.31	27.7 µM	2.59E-5		9	8.12E-6
15	SGTC_2393	9031399	4.28	185.9 µM	2.95E-5	RNA processing & uracil transport	20	9.42E-6
16	SGTC_2397	5161689	4.26	200.0 µM	3.12E-5		24	1.00E-5
17	SGTC_1683	st010134	4.24	710.0 nM	3.38E-5		19	1.10E-5
18	SGTC_2320	7373003	4.21	200.0 µM	3.83E-5		22	1.27E-5
19	SGTC_2407	paclitaxel	4.17	400.0 µM	4.50E-5		28	1.52E-5
20	SGTC_896	0964-0007	4.10	44.5 µM	5.88E-5		14	2.06E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.107	5.13E-10	YDR196C	CAB5	het	Probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; null mutant lethality is complemented by E. coli coaE (encoding DPCK); detected in purified mitochondria in high-throughput studies
0.101	4.50E-9	YGR154C	GTO1	hom	Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization
0.096	2.87E-8	YNL179C_d	YNL179C_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance
0.091	1.18E-7	YLL048C	YBT1	hom	Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
0.090	1.69E-7	YCL022C_d	YCL022C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W
0.089	2.22E-7	YLL041C	SDH2	hom	Iron-sulfur protein subunit of succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p), which couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain
0.089	2.31E-7	YOL057W	YOL057W	hom	Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers
0.088	3.62E-7	YNL055C	POR1	hom	Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication
0.085	9.41E-7	YLR215C	CDC123	het	Protein involved in nutritional control of the cell cycle; regulates abundance of the translation initiation factor eIF2; ortholog of human D123 protein
0.081	2.44E-6	YKL077W_p	YKL077W_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.079	4.06E-6	YBR259W	YBR259W	hom	Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress
0.078	5.40E-6	YLR180W	SAM1	hom	S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication
0.078	6.39E-6	YDR513W	GRX2	hom	Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has a paralog, GRX1, that arose from the whole genome duplication
0.077	9.00E-6	YGR256W	GND2	hom	6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication
0.076	1.06E-5	YPR180W	AOS1	het	Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability