YLR291C / GCD7

Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR291C / GCD7

Click on Significant Values for Screen Details ID:SGTC_87|Compound:0100-0052|FD-Score:4.48|P-value:1.51E-6 ID:SGTC_207|Compound:4185-0010|FD-Score:3.60|P-value:8.51E-5 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:16.70|P-value:2.83E-67 ID:SGTC_393|Compound:acivicin|FD-Score:-5.48|P-value:9.23E-9 ID:SGTC_539|Compound:4859-0462|FD-Score:4.32|P-value:3.35E-6 ID:SGTC_595|Compound:3909-8003|FD-Score:3.01|P-value:8.43E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:4.12|P-value:8.80E-6 ID:SGTC_627|Compound:1391-0720|FD-Score:3.33|P-value:2.55E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:4.00|P-value:1.57E-5 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.00|P-value:8.64E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:6.32|P-value:2.55E-11 ID:SGTC_1134|Compound:3477-0009|FD-Score:8.00|P-value:5.16E-17 ID:SGTC_1872|Compound:amphotericin b|FD-Score:8.27|P-value:4.69E-18 ID:SGTC_1976|Compound:st070609|FD-Score:3.07|P-value:6.81E-4 ID:SGTC_2004|Compound:4023728|FD-Score:3.27|P-value:3.23E-4 ID:SGTC_2332|Compound:7982454|FD-Score:3.40|P-value:1.94E-4 ID:SGTC_2333|Compound:9017922|FD-Score:4.44|P-value:1.90E-6 ID:SGTC_2381|Compound:9035592|FD-Score:3.16|P-value:4.79E-4 ID:SGTC_2384|Compound:9036245|FD-Score:-3.76|P-value:5.88E-5 ID:SGTC_2387|Compound:9077560|FD-Score:5.73|P-value:1.27E-9 ID:SGTC_2411|Compound:st077133|FD-Score:13.50|P-value:1.54E-44 ID:SGTC_2430|Compound:5571206|FD-Score:6.57|P-value:4.43E-12 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:-3.10|P-value:7.79E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-3.67|P-value:8.54E-5 ID:SGTC_2612|Compound:dictamnine|FD-Score:7.79|P-value:3.15E-16 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.26|P-value:2.31E-8 ID:SGTC_2880|Compound:9042983|FD-Score:3.13|P-value:5.38E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-3.31|P-value:3.60E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.20|P-value:4.23E-4 ID:SGTC_2983|Compound:9022671|FD-Score:-3.51|P-value:1.63E-4 ID:SGTC_3041|Compound:9091929|FD-Score:-3.37|P-value:2.80E-4 ID:SGTC_3047|Compound:9092122|FD-Score:3.05|P-value:7.33E-4 ID:SGTC_3075|Compound:9116269|FD-Score:-3.40|P-value:2.55E-4 ID:SGTC_3077|Compound:9117319|FD-Score:4.70|P-value:5.08E-7 ID:SGTC_3109|Compound:9122853|FD-Score:3.05|P-value:7.08E-4 ID:SGTC_3180|Compound:9109287|FD-Score:-3.21|P-value:5.16E-4 ID:SGTC_3223|Compound:9129970|FD-Score:4.37|P-value:2.65E-6 ID:SGTC_3317|Compound:9138495|FD-Score:3.12|P-value:5.55E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:4.48|P-value:1.51E-6 ID:SGTC_207|Compound:4185-0010|FD-Score:3.60|P-value:8.51E-5 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:16.70|P-value:2.83E-67 ID:SGTC_393|Compound:acivicin|FD-Score:-5.48|P-value:9.23E-9 ID:SGTC_539|Compound:4859-0462|FD-Score:4.32|P-value:3.35E-6 ID:SGTC_595|Compound:3909-8003|FD-Score:3.01|P-value:8.43E-4 ID:SGTC_611|Compound:3453-1652|FD-Score:4.12|P-value:8.80E-6 ID:SGTC_627|Compound:1391-0720|FD-Score:3.33|P-value:2.55E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:4.00|P-value:1.57E-5 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.00|P-value:8.64E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:6.32|P-value:2.55E-11 ID:SGTC_1134|Compound:3477-0009|FD-Score:8.00|P-value:5.16E-17 ID:SGTC_1872|Compound:amphotericin b|FD-Score:8.27|P-value:4.69E-18 ID:SGTC_1976|Compound:st070609|FD-Score:3.07|P-value:6.81E-4 ID:SGTC_2004|Compound:4023728|FD-Score:3.27|P-value:3.23E-4 ID:SGTC_2332|Compound:7982454|FD-Score:3.40|P-value:1.94E-4 ID:SGTC_2333|Compound:9017922|FD-Score:4.44|P-value:1.90E-6 ID:SGTC_2381|Compound:9035592|FD-Score:3.16|P-value:4.79E-4 ID:SGTC_2384|Compound:9036245|FD-Score:-3.76|P-value:5.88E-5 ID:SGTC_2387|Compound:9077560|FD-Score:5.73|P-value:1.27E-9 ID:SGTC_2411|Compound:st077133|FD-Score:13.50|P-value:1.54E-44 ID:SGTC_2430|Compound:5571206|FD-Score:6.57|P-value:4.43E-12 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:-3.10|P-value:7.79E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-3.67|P-value:8.54E-5 ID:SGTC_2612|Compound:dictamnine|FD-Score:7.79|P-value:3.15E-16 ID:SGTC_2679|Compound:rapamycin|FD-Score:5.26|P-value:2.31E-8 ID:SGTC_2880|Compound:9042983|FD-Score:3.13|P-value:5.38E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-3.31|P-value:3.60E-4 ID:SGTC_2949|Compound:9072542|FD-Score:3.20|P-value:4.23E-4 ID:SGTC_2983|Compound:9022671|FD-Score:-3.51|P-value:1.63E-4 ID:SGTC_3041|Compound:9091929|FD-Score:-3.37|P-value:2.80E-4 ID:SGTC_3047|Compound:9092122|FD-Score:3.05|P-value:7.33E-4 ID:SGTC_3075|Compound:9116269|FD-Score:-3.40|P-value:2.55E-4 ID:SGTC_3077|Compound:9117319|FD-Score:4.70|P-value:5.08E-7 ID:SGTC_3109|Compound:9122853|FD-Score:3.05|P-value:7.08E-4 ID:SGTC_3180|Compound:9109287|FD-Score:-3.21|P-value:5.16E-4 ID:SGTC_3223|Compound:9129970|FD-Score:4.37|P-value:2.65E-6 ID:SGTC_3317|Compound:9138495|FD-Score:3.12|P-value:5.55E-4

Top fitness defect scores for YLR291C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_392 N-ethylmaleimide 16.70 8.3 µM 2.83E-67 RNA pol III & RNase P/MRP 1 6.59E-63 5.03
2 SGTC_2411 st077133 13.50 39.9 µM 1.54E-44 RNA pol III & RNase P/MRP 6 1.19E-41 2.59
3 SGTC_1872 amphotericin b 8.27 148.0 nM 4.69E-18 RNA pol III & RNase P/MRP 8 6.60E-17 0.76
4 SGTC_1134 3477-0009 8.00 42.5 µM 5.16E-17 2 6.20E-16 1.30
5 SGTC_2612 dictamnine 7.79 15.5 µM 3.15E-16 RNA pol III & RNase P/MRP 13 3.36E-15 0.39
6 SGTC_2430 5571206 6.57 200.0 µM 4.43E-12 6 2.50E-11 0.46
7 SGTC_1130 3454-2858 6.32 15.9 µM 2.55E-11 NEO1 4 1.28E-10 0.49
8 SGTC_2387 9077560 5.73 195.1 µM 1.27E-9 5 4.91E-9 0.37
9 SGTC_2679 rapamycin 5.26 1.0 nM 2.31E-8 31 7.37E-8 0.02
10 SGTC_3077 9117319 4.70 49.5 µM 5.08E-7 5 1.32E-6 0.36
11 SGTC_87 0100-0052 4.48 35.5 µM 1.51E-6 14 3.65E-6 0.28
12 SGTC_2333 9017922 4.44 200.0 µM 1.90E-6 4 4.52E-6 0.19
13 SGTC_3223 9129970 4.37 49.5 µM 2.65E-6 3 6.14E-6 0.27
14 SGTC_539 4859-0462 4.32 2.5 µM 3.35E-6 RNA pol III & RNase P/MRP 17 7.65E-6 0.04
15 SGTC_611 3453-1652 4.12 23.7 µM 8.80E-6 20 1.88E-5 0.14
16 SGTC_705 1124-0212 4.00 310.0 µM 1.57E-5 6 3.23E-5 0.09
17 SGTC_207 4185-0010 3.60 4.5 µM 8.51E-5 endomembrane recycling 19 1.56E-4 0.06
18 SGTC_2332 7982454 3.40 138.9 µM 1.94E-4 16 3.37E-4 0.09
19 SGTC_627 1391-0720 3.33 175.0 µM 2.55E-4 8 4.34E-4 0.02
20 SGTC_2004 4023728 3.27 41.9 µM 3.23E-4 37 5.42E-4 0.00

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.515 1.08E-226 YGR083C GCD2 het Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.444 6.51E-162 YNL221C POP1 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.411 5.37E-137 YOR260W GCD1 het Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.331 1.22E-86 YDR211W GCD6 het Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress
0.236 1.26E-43 YBR167C POP7 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
0.200 1.56E-31 YOR207C RET1 het Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.184 7.14E-27 YMR283C RIT1 hom 2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA
0.181 4.78E-26 YBR257W POP4 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.179 1.46E-25 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.174 3.27E-24 YDL152W_d YDL152W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
0.171 2.05E-23 YDL150W RPC53 het RNA polymerase III subunit C53
0.168 1.22E-22 YLR243W GPN3 het Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
0.165 6.64E-22 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.138 1.20E-15 YDR527W RBA50 het Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
0.130 4.45E-14 YHR021W-A_p ECM12_p hom Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity