YLR303W / MET17

Methionine and cysteine synthase (O-acetyl homoserine-O-acetyl serine sulfhydrylase), required for sulfur amino acid synthesis

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR303W / MET17

Click on Significant Values for Screen Details ID:SGTC_47|Compound:0149-0135|FD-Score:3.66|P-value:2.64E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:4.15|P-value:4.45E-5 ID:SGTC_165|Compound:k038-0023|FD-Score:4.84|P-value:2.40E-6 ID:SGTC_274|Compound:sirtinol|FD-Score:4.34|P-value:2.04E-5 ID:SGTC_302|Compound:1094-0063|FD-Score:4.87|P-value:2.11E-6 ID:SGTC_348|Compound:k064-0012|FD-Score:4.72|P-value:4.09E-6 ID:SGTC_378|Compound:3970-0959|FD-Score:3.70|P-value:2.35E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:3.40|P-value:6.36E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:4.42|P-value:1.48E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:4.19|P-value:3.73E-5 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:3.68|P-value:2.45E-4 ID:SGTC_485|Compound:niflumic acid|FD-Score:3.69|P-value:2.41E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:3.54|P-value:4.00E-4 ID:SGTC_654|Compound:1124-0271|FD-Score:3.38|P-value:6.88E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:3.84|P-value:1.43E-4 ID:SGTC_766|Compound:1391-0721|FD-Score:3.87|P-value:1.24E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:-3.34|P-value:9.57E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:6.05|P-value:5.68E-9 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:4.52|P-value:9.68E-6 ID:SGTC_984|Compound:1492-0255|FD-Score:3.76|P-value:1.87E-4 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:3.70|P-value:2.33E-4 ID:SGTC_1170|Compound:0310-0127|FD-Score:4.36|P-value:1.93E-5 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.56|P-value:3.74E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:-4.06|P-value:8.06E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.97|P-value:8.71E-5 ID:SGTC_1253|Compound:0550-0034|FD-Score:4.91|P-value:1.80E-6 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:3.78|P-value:1.75E-4 ID:SGTC_1671|Compound:st016596|FD-Score:3.53|P-value:4.16E-4 ID:SGTC_1696|Compound:st025591|FD-Score:3.28|P-value:9.51E-4 ID:SGTC_1743|Compound:st037606|FD-Score:6.01|P-value:7.44E-9 ID:SGTC_1749|Compound:st037932|FD-Score:5.65|P-value:5.02E-8 ID:SGTC_1752|Compound:st044819|FD-Score:3.56|P-value:3.71E-4 ID:SGTC_1767|Compound:st044992|FD-Score:3.59|P-value:3.41E-4 ID:SGTC_1851|Compound:st056236|FD-Score:-3.75|P-value:2.43E-4 ID:SGTC_1909|Compound:st060837|FD-Score:4.61|P-value:6.72E-6 ID:SGTC_1913|Compound:st060213|FD-Score:3.27|P-value:9.68E-4 ID:SGTC_1958|Compound:st076513|FD-Score:4.67|P-value:5.20E-6 ID:SGTC_1990|Compound:st072050|FD-Score:3.62|P-value:3.08E-4 ID:SGTC_1995|Compound:st072814|FD-Score:4.80|P-value:2.92E-6 ID:SGTC_2017|Compound:5132927|FD-Score:4.14|P-value:4.58E-5 ID:SGTC_2067|Compound:5224629|FD-Score:3.44|P-value:5.54E-4 ID:SGTC_2087|Compound:5276107|FD-Score:3.58|P-value:3.49E-4 ID:SGTC_2183|Compound:6420625|FD-Score:3.59|P-value:3.40E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:-3.62|P-value:3.82E-4 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:5.02|P-value:1.09E-6 ID:SGTC_2547|Compound:epiafzelechin trimethyl ether|FD-Score:-3.50|P-value:5.81E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.49|P-value:4.73E-4 ID:SGTC_2641|Compound:levomentholum|FD-Score:-3.70|P-value:2.87E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:4.05|P-value:6.38E-5 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:6.39|P-value:8.38E-10 ID:SGTC_2773|Compound:thioridazine|FD-Score:3.80|P-value:1.61E-4 ID:SGTC_2805|Compound:7950783|FD-Score:4.37|P-value:1.82E-5 ID:SGTC_2938|Compound:9059746|FD-Score:3.91|P-value:1.10E-4 ID:SGTC_2982|Compound:9019404|FD-Score:4.10|P-value:5.26E-5 ID:SGTC_3000|Compound:9075062|FD-Score:3.33|P-value:8.07E-4 ID:SGTC_3017|Compound:9080681|FD-Score:-4.32|P-value:2.89E-5 ID:SGTC_3041|Compound:9091929|FD-Score:3.69|P-value:2.38E-4 ID:SGTC_3061|Compound:glucosamine|FD-Score:-3.65|P-value:3.44E-4 ID:SGTC_3123|Compound:9126162|FD-Score:5.28|P-value:3.10E-7 ID:SGTC_3159|Compound:9100096|FD-Score:3.48|P-value:4.94E-4 ID:SGTC_3196|Compound:9111891|FD-Score:3.67|P-value:2.61E-4 ID:SGTC_3269|Compound:9137823|FD-Score:-3.86|P-value:1.67E-4 ID:SGTC_47|Compound:0149-0135|FD-Score:3.66|P-value:2.64E-4 ID:SGTC_160|Compound:3453-0671|FD-Score:4.15|P-value:4.45E-5 ID:SGTC_165|Compound:k038-0023|FD-Score:4.84|P-value:2.40E-6 ID:SGTC_274|Compound:sirtinol|FD-Score:4.34|P-value:2.04E-5 ID:SGTC_302|Compound:1094-0063|FD-Score:4.87|P-value:2.11E-6 ID:SGTC_348|Compound:k064-0012|FD-Score:4.72|P-value:4.09E-6 ID:SGTC_378|Compound:3970-0959|FD-Score:3.70|P-value:2.35E-4 ID:SGTC_404|Compound:fumagillin|FD-Score:3.40|P-value:6.36E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:4.42|P-value:1.48E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:4.19|P-value:3.73E-5 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:3.68|P-value:2.45E-4 ID:SGTC_485|Compound:niflumic acid|FD-Score:3.69|P-value:2.41E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:3.54|P-value:4.00E-4 ID:SGTC_654|Compound:1124-0271|FD-Score:3.38|P-value:6.88E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:3.84|P-value:1.43E-4 ID:SGTC_766|Compound:1391-0721|FD-Score:3.87|P-value:1.24E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:-3.34|P-value:9.57E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:6.05|P-value:5.68E-9 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:4.52|P-value:9.68E-6 ID:SGTC_984|Compound:1492-0255|FD-Score:3.76|P-value:1.87E-4 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:3.70|P-value:2.33E-4 ID:SGTC_1170|Compound:0310-0127|FD-Score:4.36|P-value:1.93E-5 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.56|P-value:3.74E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:-4.06|P-value:8.06E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.97|P-value:8.71E-5 ID:SGTC_1253|Compound:0550-0034|FD-Score:4.91|P-value:1.80E-6 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:3.78|P-value:1.75E-4 ID:SGTC_1671|Compound:st016596|FD-Score:3.53|P-value:4.16E-4 ID:SGTC_1696|Compound:st025591|FD-Score:3.28|P-value:9.51E-4 ID:SGTC_1743|Compound:st037606|FD-Score:6.01|P-value:7.44E-9 ID:SGTC_1749|Compound:st037932|FD-Score:5.65|P-value:5.02E-8 ID:SGTC_1752|Compound:st044819|FD-Score:3.56|P-value:3.71E-4 ID:SGTC_1767|Compound:st044992|FD-Score:3.59|P-value:3.41E-4 ID:SGTC_1851|Compound:st056236|FD-Score:-3.75|P-value:2.43E-4 ID:SGTC_1909|Compound:st060837|FD-Score:4.61|P-value:6.72E-6 ID:SGTC_1913|Compound:st060213|FD-Score:3.27|P-value:9.68E-4 ID:SGTC_1958|Compound:st076513|FD-Score:4.67|P-value:5.20E-6 ID:SGTC_1990|Compound:st072050|FD-Score:3.62|P-value:3.08E-4 ID:SGTC_1995|Compound:st072814|FD-Score:4.80|P-value:2.92E-6 ID:SGTC_2017|Compound:5132927|FD-Score:4.14|P-value:4.58E-5 ID:SGTC_2067|Compound:5224629|FD-Score:3.44|P-value:5.54E-4 ID:SGTC_2087|Compound:5276107|FD-Score:3.58|P-value:3.49E-4 ID:SGTC_2183|Compound:6420625|FD-Score:3.59|P-value:3.40E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:-3.62|P-value:3.82E-4 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:5.02|P-value:1.09E-6 ID:SGTC_2547|Compound:epiafzelechin trimethyl ether|FD-Score:-3.50|P-value:5.81E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.49|P-value:4.73E-4 ID:SGTC_2641|Compound:levomentholum|FD-Score:-3.70|P-value:2.87E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:4.05|P-value:6.38E-5 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:6.39|P-value:8.38E-10 ID:SGTC_2773|Compound:thioridazine|FD-Score:3.80|P-value:1.61E-4 ID:SGTC_2805|Compound:7950783|FD-Score:4.37|P-value:1.82E-5 ID:SGTC_2938|Compound:9059746|FD-Score:3.91|P-value:1.10E-4 ID:SGTC_2982|Compound:9019404|FD-Score:4.10|P-value:5.26E-5 ID:SGTC_3000|Compound:9075062|FD-Score:3.33|P-value:8.07E-4 ID:SGTC_3017|Compound:9080681|FD-Score:-4.32|P-value:2.89E-5 ID:SGTC_3041|Compound:9091929|FD-Score:3.69|P-value:2.38E-4 ID:SGTC_3061|Compound:glucosamine|FD-Score:-3.65|P-value:3.44E-4 ID:SGTC_3123|Compound:9126162|FD-Score:5.28|P-value:3.10E-7 ID:SGTC_3159|Compound:9100096|FD-Score:3.48|P-value:4.94E-4 ID:SGTC_3196|Compound:9111891|FD-Score:3.67|P-value:2.61E-4 ID:SGTC_3269|Compound:9137823|FD-Score:-3.86|P-value:1.67E-4

Top fitness defect scores for YLR303W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2758 dehydroepiandrosterone 6.39 69.3 µM 8.38E-10 18 8.13E-11
2 SGTC_907 3448-6986 6.05 75.5 µM 5.68E-9 2 7.03E-10
3 SGTC_1743 st037606 6.01 46.4 µM 7.44E-9 2 9.54E-10
4 SGTC_1749 st037932 5.65 54.2 µM 5.02E-8 10 8.21E-9
5 SGTC_3123 9126162 5.28 49.5 µM 3.10E-7 7 6.36E-8
6 SGTC_2501 sodium tetradecyl sulfate 5.02 13.4 µM 1.09E-6 16 2.63E-7
7 SGTC_1253 0550-0034 4.91 176.0 µM 1.80E-6 8 4.62E-7
8 SGTC_302 1094-0063 4.87 18.9 µM 2.11E-6 3 5.50E-7
9 SGTC_165 k038-0023 4.84 64.4 µM 2.40E-6 ubiquinone biosynthesis & proteasome 46 6.36E-7
10 SGTC_1995 st072814 4.80 61.1 µM 2.92E-6 4 7.94E-7
11 SGTC_348 k064-0012 4.72 10.7 µM 4.09E-6 60S ribosome export 8 1.16E-6
12 SGTC_1958 st076513 4.67 41.9 µM 5.20E-6 60S ribosome export 18 1.52E-6
13 SGTC_1909 st060837 4.61 51.5 µM 6.72E-6 34 2.03E-6
14 SGTC_920 mycophenolic acid 4.52 780.4 nM 9.68E-6 17 3.06E-6
15 SGTC_430 0958-0115 4.42 16.4 µM 1.48E-5 17 4.92E-6
16 SGTC_2805 7950783 4.37 58.4 µM 1.82E-5 21 6.21E-6
17 SGTC_1170 0310-0127 4.36 64.8 µM 1.93E-5 14 6.62E-6
18 SGTC_274 sirtinol 4.34 40.1 µM 2.04E-5 14 7.06E-6
19 SGTC_431 k089-0097 4.19 107.0 µM 3.73E-5 iron homeostasis 33 1.39E-5
20 SGTC_160 3453-0671 4.15 289.7 µM 4.45E-5 37 1.69E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.100 7.33E-9 YGR279C SCW4 hom Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating
0.095 3.64E-8 YPR134W MSS18 hom Nuclear encoded protein needed for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions
0.089 2.30E-7 YAL018C_p YAL018C_p hom Putative protein of unknown function
0.081 2.93E-6 YLR279W_d YLR279W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.080 3.71E-6 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.080 4.00E-6 YGL015C_p YGL015C_p hom Putative protein of unknown function; null mutants accumulate cargo in the Golgi
0.078 6.34E-6 YML034C-A_d YML034C-A_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.077 7.02E-6 YLR316C TAD3 het Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs
0.076 9.47E-6 YDL210W UGA4 hom Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane
0.075 1.47E-5 YOL052C-A DDR2 hom Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication
0.071 3.52E-5 YER184C_p YER184C_p hom Putative zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source
0.070 5.52E-5 YGR034W RPL26B hom Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication
0.069 6.27E-5 YMR254C_d YMR254C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.069 6.47E-5 YER069W ARG5,6 hom Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
0.067 9.58E-5 YPL162C_p YPL162C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology