YLR304C / ACO1

Aconitase, required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; phosphorylated; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR304C / ACO1

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:6.25|P-value:4.07E-8 ID:SGTC_31|Compound:0508-1974|FD-Score:4.23|P-value:1.61E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:4.83|P-value:1.85E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:3.85|P-value:5.42E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:3.77|P-value:6.91E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:7.44|P-value:7.42E-11 ID:SGTC_58|Compound:0957-0393|FD-Score:5.08|P-value:7.07E-6 ID:SGTC_86|Compound:0417-1630|FD-Score:6.55|P-value:8.98E-9 ID:SGTC_149|Compound:3970-0822|FD-Score:4.44|P-value:7.89E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:3.92|P-value:4.45E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:4.50|P-value:6.33E-5 ID:SGTC_195|Compound:4234-0266|FD-Score:3.93|P-value:4.30E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:4.27|P-value:1.39E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:8.68|P-value:3.34E-14 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:4.25|P-value:1.49E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:9.56|P-value:7.32E-17 ID:SGTC_304|Compound:1310-0298|FD-Score:4.37|P-value:1.00E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:-4.59|P-value:1.89E-5 ID:SGTC_374|Compound:0880-0231|FD-Score:-3.69|P-value:4.28E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:4.26|P-value:1.43E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.03|P-value:3.39E-6 ID:SGTC_444|Compound:curcumin|FD-Score:-4.28|P-value:5.94E-5 ID:SGTC_596|Compound:1155-0152|FD-Score:6.19|P-value:5.45E-8 ID:SGTC_748|Compound:0136-0228|FD-Score:6.54|P-value:9.53E-9 ID:SGTC_752|Compound:1896-0090|FD-Score:3.71|P-value:8.27E-4 ID:SGTC_767|Compound:0929-0023|FD-Score:3.67|P-value:9.33E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:5.35|P-value:2.30E-6 ID:SGTC_908|Compound:3448-7491|FD-Score:4.83|P-value:1.87E-5 ID:SGTC_989|Compound:1501-0895|FD-Score:-3.96|P-value:1.79E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:4.04|P-value:3.04E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:5.07|P-value:7.39E-6 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.83|P-value:1.86E-5 ID:SGTC_1094|Compound:duloxetine|FD-Score:4.22|P-value:1.68E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:-4.56|P-value:2.13E-5 ID:SGTC_1112|Compound:tnp00069|FD-Score:4.60|P-value:4.41E-5 ID:SGTC_1188|Compound:1403-0014|FD-Score:13.70|P-value:1.04E-32 ID:SGTC_1191|Compound:1487-1166|FD-Score:-3.62|P-value:5.44E-4 ID:SGTC_1228|Compound:0124-0032|FD-Score:4.14|P-value:2.20E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:6.00|P-value:1.33E-7 ID:SGTC_1315|Compound:1237-0019|FD-Score:4.10|P-value:2.45E-4 ID:SGTC_1391|Compound:3442-0225|FD-Score:4.70|P-value:3.08E-5 ID:SGTC_1521|Compound:st012942|FD-Score:4.29|P-value:1.30E-4 ID:SGTC_1635|Compound:st007035|FD-Score:8.15|P-value:1.07E-12 ID:SGTC_1637|Compound:st007924|FD-Score:4.80|P-value:2.06E-5 ID:SGTC_1742|Compound:st038235|FD-Score:6.29|P-value:3.38E-8 ID:SGTC_1750|Compound:st081859|FD-Score:6.36|P-value:2.32E-8 ID:SGTC_1787|Compound:fluconazole|FD-Score:-5.32|P-value:9.93E-7 ID:SGTC_1860|Compound:st056228|FD-Score:4.21|P-value:1.70E-4 ID:SGTC_2141|Compound:5621638|FD-Score:-3.47|P-value:8.54E-4 ID:SGTC_2183|Compound:6420625|FD-Score:5.82|P-value:3.08E-7 ID:SGTC_2191|Compound:5911152|FD-Score:4.01|P-value:3.30E-4 ID:SGTC_2196|Compound:6573537|FD-Score:-4.13|P-value:1.01E-4 ID:SGTC_2200|Compound:6600604|FD-Score:-3.48|P-value:8.14E-4 ID:SGTC_2223|Compound:6903751|FD-Score:-3.86|P-value:2.51E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:6.60|P-value:7.05E-9 ID:SGTC_2250|Compound:alverine citrate|FD-Score:7.89|P-value:5.37E-12 ID:SGTC_2254|Compound:7938975|FD-Score:4.56|P-value:5.07E-5 ID:SGTC_2326|Compound:6164455|FD-Score:5.63|P-value:7.13E-7 ID:SGTC_2337|Compound:9009472|FD-Score:3.70|P-value:8.53E-4 ID:SGTC_2401|Compound:5128683|FD-Score:3.75|P-value:7.35E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:6.57|P-value:8.13E-9 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:11.80|P-value:7.41E-25 ID:SGTC_2513|Compound:baicalein|FD-Score:14.00|P-value:3.81E-34 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.09|P-value:6.71E-6 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:11.00|P-value:6.94E-22 ID:SGTC_2619|Compound:obliquin|FD-Score:5.99|P-value:1.39E-7 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.14|P-value:2.16E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:4.10|P-value:2.43E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:4.22|P-value:1.64E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:4.02|P-value:3.25E-4 ID:SGTC_2791|Compound:5663188|FD-Score:-4.66|P-value:1.48E-5 ID:SGTC_2816|Compound:7982706|FD-Score:-4.76|P-value:1.00E-5 ID:SGTC_2823|Compound:7987624|FD-Score:4.04|P-value:2.98E-4 ID:SGTC_2954|Compound:9074818|FD-Score:9.00|P-value:3.96E-15 ID:SGTC_2980|Compound:9014342|FD-Score:4.01|P-value:3.27E-4 ID:SGTC_2996|Compound:9066192|FD-Score:4.57|P-value:4.85E-5 ID:SGTC_3016|Compound:9080866|FD-Score:3.75|P-value:7.50E-4 ID:SGTC_3142|Compound:9086498|FD-Score:-6.42|P-value:5.31E-9 ID:SGTC_3178|Compound:9107506|FD-Score:3.96|P-value:3.81E-4 ID:SGTC_3276|Compound:9139060|FD-Score:-4.94|P-value:4.87E-6 ID:SGTC_10|Compound:0279-0212|FD-Score:6.25|P-value:4.07E-8 ID:SGTC_31|Compound:0508-1974|FD-Score:4.23|P-value:1.61E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:4.83|P-value:1.85E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:3.85|P-value:5.42E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:3.77|P-value:6.91E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:7.44|P-value:7.42E-11 ID:SGTC_58|Compound:0957-0393|FD-Score:5.08|P-value:7.07E-6 ID:SGTC_86|Compound:0417-1630|FD-Score:6.55|P-value:8.98E-9 ID:SGTC_149|Compound:3970-0822|FD-Score:4.44|P-value:7.89E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:3.92|P-value:4.45E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:4.50|P-value:6.33E-5 ID:SGTC_195|Compound:4234-0266|FD-Score:3.93|P-value:4.30E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:4.27|P-value:1.39E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:8.68|P-value:3.34E-14 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:4.25|P-value:1.49E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:9.56|P-value:7.32E-17 ID:SGTC_304|Compound:1310-0298|FD-Score:4.37|P-value:1.00E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:-4.59|P-value:1.89E-5 ID:SGTC_374|Compound:0880-0231|FD-Score:-3.69|P-value:4.28E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:4.26|P-value:1.43E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-5.03|P-value:3.39E-6 ID:SGTC_444|Compound:curcumin|FD-Score:-4.28|P-value:5.94E-5 ID:SGTC_596|Compound:1155-0152|FD-Score:6.19|P-value:5.45E-8 ID:SGTC_748|Compound:0136-0228|FD-Score:6.54|P-value:9.53E-9 ID:SGTC_752|Compound:1896-0090|FD-Score:3.71|P-value:8.27E-4 ID:SGTC_767|Compound:0929-0023|FD-Score:3.67|P-value:9.33E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:5.35|P-value:2.30E-6 ID:SGTC_908|Compound:3448-7491|FD-Score:4.83|P-value:1.87E-5 ID:SGTC_989|Compound:1501-0895|FD-Score:-3.96|P-value:1.79E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:4.04|P-value:3.04E-4 ID:SGTC_1060|Compound:3448-7350|FD-Score:5.07|P-value:7.39E-6 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.83|P-value:1.86E-5 ID:SGTC_1094|Compound:duloxetine|FD-Score:4.22|P-value:1.68E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:-4.56|P-value:2.13E-5 ID:SGTC_1112|Compound:tnp00069|FD-Score:4.60|P-value:4.41E-5 ID:SGTC_1188|Compound:1403-0014|FD-Score:13.70|P-value:1.04E-32 ID:SGTC_1191|Compound:1487-1166|FD-Score:-3.62|P-value:5.44E-4 ID:SGTC_1228|Compound:0124-0032|FD-Score:4.14|P-value:2.20E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:6.00|P-value:1.33E-7 ID:SGTC_1315|Compound:1237-0019|FD-Score:4.10|P-value:2.45E-4 ID:SGTC_1391|Compound:3442-0225|FD-Score:4.70|P-value:3.08E-5 ID:SGTC_1521|Compound:st012942|FD-Score:4.29|P-value:1.30E-4 ID:SGTC_1635|Compound:st007035|FD-Score:8.15|P-value:1.07E-12 ID:SGTC_1637|Compound:st007924|FD-Score:4.80|P-value:2.06E-5 ID:SGTC_1742|Compound:st038235|FD-Score:6.29|P-value:3.38E-8 ID:SGTC_1750|Compound:st081859|FD-Score:6.36|P-value:2.32E-8 ID:SGTC_1787|Compound:fluconazole|FD-Score:-5.32|P-value:9.93E-7 ID:SGTC_1860|Compound:st056228|FD-Score:4.21|P-value:1.70E-4 ID:SGTC_2141|Compound:5621638|FD-Score:-3.47|P-value:8.54E-4 ID:SGTC_2183|Compound:6420625|FD-Score:5.82|P-value:3.08E-7 ID:SGTC_2191|Compound:5911152|FD-Score:4.01|P-value:3.30E-4 ID:SGTC_2196|Compound:6573537|FD-Score:-4.13|P-value:1.01E-4 ID:SGTC_2200|Compound:6600604|FD-Score:-3.48|P-value:8.14E-4 ID:SGTC_2223|Compound:6903751|FD-Score:-3.86|P-value:2.51E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:6.60|P-value:7.05E-9 ID:SGTC_2250|Compound:alverine citrate|FD-Score:7.89|P-value:5.37E-12 ID:SGTC_2254|Compound:7938975|FD-Score:4.56|P-value:5.07E-5 ID:SGTC_2326|Compound:6164455|FD-Score:5.63|P-value:7.13E-7 ID:SGTC_2337|Compound:9009472|FD-Score:3.70|P-value:8.53E-4 ID:SGTC_2401|Compound:5128683|FD-Score:3.75|P-value:7.35E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:6.57|P-value:8.13E-9 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:11.80|P-value:7.41E-25 ID:SGTC_2513|Compound:baicalein|FD-Score:14.00|P-value:3.81E-34 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.09|P-value:6.71E-6 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:11.00|P-value:6.94E-22 ID:SGTC_2619|Compound:obliquin|FD-Score:5.99|P-value:1.39E-7 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.14|P-value:2.16E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:4.10|P-value:2.43E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:4.22|P-value:1.64E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:4.02|P-value:3.25E-4 ID:SGTC_2791|Compound:5663188|FD-Score:-4.66|P-value:1.48E-5 ID:SGTC_2816|Compound:7982706|FD-Score:-4.76|P-value:1.00E-5 ID:SGTC_2823|Compound:7987624|FD-Score:4.04|P-value:2.98E-4 ID:SGTC_2954|Compound:9074818|FD-Score:9.00|P-value:3.96E-15 ID:SGTC_2980|Compound:9014342|FD-Score:4.01|P-value:3.27E-4 ID:SGTC_2996|Compound:9066192|FD-Score:4.57|P-value:4.85E-5 ID:SGTC_3016|Compound:9080866|FD-Score:3.75|P-value:7.50E-4 ID:SGTC_3142|Compound:9086498|FD-Score:-6.42|P-value:5.31E-9 ID:SGTC_3178|Compound:9107506|FD-Score:3.96|P-value:3.81E-4 ID:SGTC_3276|Compound:9139060|FD-Score:-4.94|P-value:4.87E-6

Top fitness defect scores for YLR304C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2513 baicalein 14.00 25.6 µM 3.81E-34 calcium & mitochondrial duress 1 1.07E-44
2 SGTC_1188 1403-0014 13.70 4.6 µM 1.04E-32 mitochondrial stress 1 8.26E-43
3 SGTC_2509 methyl 7-deshydroxypyrogallin-4-carboxylate 11.80 54.6 µM 7.41E-25 RPP1 & pyrimidine depletion 1 1.78E-32
4 SGTC_2615 purpurogallin-4-carboxylic acid 11.00 100.0 µM 6.94E-22 redox potentiating 6 1.45E-28
5 SGTC_275 trichlorophene 9.56 5.7 µM 7.32E-17 mitochondrial stress 51 6.03E-22
6 SGTC_2954 9074818 9.00 52.7 µM 3.96E-15 3 1.16E-19
7 SGTC_236 1509-0011 8.68 5.3 µM 3.34E-14 229 1.93E-18
8 SGTC_1635 st007035 8.15 42.4 µM 1.07E-12 96 1.86E-16
9 SGTC_2250 alverine citrate 7.89 130.0 µM 5.37E-12 21 1.57E-15
10 SGTC_55 0767-0285 7.44 6.6 µM 7.42E-11 10 5.02E-14
11 SGTC_2232 chlorophetanol 6.60 41.5 µM 7.05E-9 TRP & mitochondrial translation 33 2.05E-11
12 SGTC_2504 purpurogallin 6.57 52.3 µM 8.13E-9 RPP1 & pyrimidine depletion 1 2.47E-11
13 SGTC_86 0417-1630 6.55 471.9 µM 8.98E-9 ubiquinone biosynthesis & proteasome 81 2.82E-11
14 SGTC_748 0136-0228 6.54 77.9 µM 9.53E-9 redox potentiating 31 3.05E-11
15 SGTC_1750 st081859 6.36 2.4 µM 2.32E-8 endomembrane recycling 101 9.89E-11
16 SGTC_1742 st038235 6.29 28.9 µM 3.38E-8 mitochondrial stress 40 1.62E-10
17 SGTC_10 0279-0212 6.25 42.6 µM 4.07E-8 TRP & mitochondrial translation 24 2.08E-10
18 SGTC_596 1155-0152 6.19 116.0 µM 5.45E-8 TRP & mitochondrial translation 9 3.05E-10
19 SGTC_1260 0700-1617 6.00 7.8 µM 1.33E-7 calcium & mitochondrial duress 8 9.87E-10
20 SGTC_2619 obliquin 5.99 87.9 µM 1.39E-7 cell wall 76 1.05E-9

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.413 1.21E-138 YLR369W SSQ1 hom Mitochondrial hsp70-type molecular chaperone, required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis, and for maturation of Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia
0.333 1.28E-87 YBR179C FZO1 hom Mitofusin; integral membrane protein involved in mitochondrial outer membrane tethering and fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system
0.331 1.23E-86 YNL252C MRPL17 hom Mitochondrial ribosomal protein of the large subunit
0.317 2.45E-79 YDR533C HSP31 hom Possible chaperone and cysteine protease; has similarity to E. coli Hsp31; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress
0.317 5.88E-79 YOR158W PET123 hom Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator
0.309 2.81E-75 YDR462W MRPL28 hom Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
0.304 1.10E-72 YDR518W EUG1 hom Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.300 5.96E-71 YGR257C MTM1 hom Mitochondrial protein of the mitochondrial carrier family, involved in activating mitochondrial Sod2p probably by facilitating insertion of an essential manganese cofactor
0.299 2.02E-70 YNL184C_p YNL184C_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.290 6.79E-66 YDR237W MRPL7 hom Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein
0.285 7.90E-64 YLL027W ISA1 hom Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins
0.281 9.80E-62 YJR122W IBA57 hom Mitochondrial matrix protein involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system
0.278 2.02E-60 YPR067W ISA2 hom Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations
0.277 4.05E-60 YGR102C GTF1 hom Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria
0.275 3.29E-59 YPR166C MRP2 hom Mitochondrial ribosomal protein of the small subunit