YLR310C / CDC25

Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR310C / CDC25

Click on Significant Values for Screen Details ID:SGTC_3|Compound:0833-0587|FD-Score:3.48|P-value:9.65E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:-4.26|P-value:8.38E-5 ID:SGTC_122|Compound:0236-0016|FD-Score:3.63|P-value:6.17E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:6.23|P-value:1.49E-8 ID:SGTC_145|Compound:3737-0113|FD-Score:4.22|P-value:8.63E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:4.74|P-value:1.22E-5 ID:SGTC_314|Compound:1671-0008|FD-Score:3.99|P-value:1.92E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:5.02|P-value:4.02E-6 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:15.80|P-value:6.68E-45 ID:SGTC_435|Compound:c-PAF|FD-Score:6.04|P-value:3.87E-8 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:9.40|P-value:3.42E-17 ID:SGTC_455|Compound:paf C-16|FD-Score:5.49|P-value:5.26E-7 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:-4.40|P-value:4.98E-5 ID:SGTC_491|Compound:tetrandrine|FD-Score:-4.12|P-value:1.38E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:4.15|P-value:1.11E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.31|P-value:6.93E-5 ID:SGTC_551|Compound:k048-0028|FD-Score:3.48|P-value:9.58E-4 ID:SGTC_555|Compound:4296-0685|FD-Score:4.26|P-value:7.58E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:5.00|P-value:4.24E-6 ID:SGTC_659|Compound:k018-0002|FD-Score:3.80|P-value:3.59E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:4.86|P-value:7.55E-6 ID:SGTC_783|Compound:0848-0141|FD-Score:4.38|P-value:4.89E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:3.50|P-value:9.20E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:3.73|P-value:4.42E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:-4.11|P-value:1.40E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:-4.07|P-value:1.60E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:3.95|P-value:2.19E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-3.61|P-value:7.27E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:4.00|P-value:1.86E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:4.33|P-value:5.87E-5 ID:SGTC_1602|Compound:st001878|FD-Score:11.70|P-value:1.07E-25 ID:SGTC_1616|Compound:st002734|FD-Score:8.39|P-value:4.34E-14 ID:SGTC_1659|Compound:st012954|FD-Score:3.73|P-value:4.44E-4 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:3.75|P-value:4.13E-4 ID:SGTC_2023|Compound:5144124|FD-Score:3.77|P-value:3.93E-4 ID:SGTC_2063|Compound:5246145|FD-Score:4.06|P-value:1.52E-4 ID:SGTC_2066|Compound:5222451|FD-Score:-3.95|P-value:2.43E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:-6.59|P-value:2.78E-9 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.23|P-value:8.17E-5 ID:SGTC_2468|Compound:5987765|FD-Score:3.53|P-value:8.31E-4 ID:SGTC_2517|Compound:totarol|FD-Score:4.79|P-value:1.01E-5 ID:SGTC_2765|Compound:myclobutanil|FD-Score:-3.65|P-value:6.42E-4 ID:SGTC_2793|Compound:7325131|FD-Score:3.73|P-value:4.46E-4 ID:SGTC_2849|Compound:9019321|FD-Score:4.12|P-value:1.22E-4 ID:SGTC_2855|Compound:9016525|FD-Score:4.05|P-value:1.56E-4 ID:SGTC_2887|Compound:9044784|FD-Score:-4.31|P-value:7.08E-5 ID:SGTC_2965|Compound:9082772|FD-Score:-4.47|P-value:3.88E-5 ID:SGTC_2974|Compound:9094440|FD-Score:3.60|P-value:6.71E-4 ID:SGTC_2976|Compound:9009005|FD-Score:4.59|P-value:2.21E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-4.08|P-value:1.59E-4 ID:SGTC_3225|Compound:9130819|FD-Score:5.70|P-value:2.04E-7 ID:SGTC_3289|Compound:9119912|FD-Score:4.16|P-value:1.08E-4 ID:SGTC_3|Compound:0833-0587|FD-Score:3.48|P-value:9.65E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:-4.26|P-value:8.38E-5 ID:SGTC_122|Compound:0236-0016|FD-Score:3.63|P-value:6.17E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:6.23|P-value:1.49E-8 ID:SGTC_145|Compound:3737-0113|FD-Score:4.22|P-value:8.63E-5 ID:SGTC_227|Compound:fluconazole|FD-Score:4.74|P-value:1.22E-5 ID:SGTC_314|Compound:1671-0008|FD-Score:3.99|P-value:1.92E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:5.02|P-value:4.02E-6 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:15.80|P-value:6.68E-45 ID:SGTC_435|Compound:c-PAF|FD-Score:6.04|P-value:3.87E-8 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:9.40|P-value:3.42E-17 ID:SGTC_455|Compound:paf C-16|FD-Score:5.49|P-value:5.26E-7 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:-4.40|P-value:4.98E-5 ID:SGTC_491|Compound:tetrandrine|FD-Score:-4.12|P-value:1.38E-4 ID:SGTC_543|Compound:3844-1095|FD-Score:4.15|P-value:1.11E-4 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.31|P-value:6.93E-5 ID:SGTC_551|Compound:k048-0028|FD-Score:3.48|P-value:9.58E-4 ID:SGTC_555|Compound:4296-0685|FD-Score:4.26|P-value:7.58E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:5.00|P-value:4.24E-6 ID:SGTC_659|Compound:k018-0002|FD-Score:3.80|P-value:3.59E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:4.86|P-value:7.55E-6 ID:SGTC_783|Compound:0848-0141|FD-Score:4.38|P-value:4.89E-5 ID:SGTC_832|Compound:4083-0019|FD-Score:3.50|P-value:9.20E-4 ID:SGTC_929|Compound:2805-0021|FD-Score:3.73|P-value:4.42E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:-4.11|P-value:1.40E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:-4.07|P-value:1.60E-4 ID:SGTC_1172|Compound:0370-0043|FD-Score:3.95|P-value:2.19E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-3.61|P-value:7.27E-4 ID:SGTC_1370|Compound:2001-0092|FD-Score:4.00|P-value:1.86E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:4.33|P-value:5.87E-5 ID:SGTC_1602|Compound:st001878|FD-Score:11.70|P-value:1.07E-25 ID:SGTC_1616|Compound:st002734|FD-Score:8.39|P-value:4.34E-14 ID:SGTC_1659|Compound:st012954|FD-Score:3.73|P-value:4.44E-4 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:3.75|P-value:4.13E-4 ID:SGTC_2023|Compound:5144124|FD-Score:3.77|P-value:3.93E-4 ID:SGTC_2063|Compound:5246145|FD-Score:4.06|P-value:1.52E-4 ID:SGTC_2066|Compound:5222451|FD-Score:-3.95|P-value:2.43E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:-6.59|P-value:2.78E-9 ID:SGTC_2419|Compound:paclitaxel|FD-Score:4.23|P-value:8.17E-5 ID:SGTC_2468|Compound:5987765|FD-Score:3.53|P-value:8.31E-4 ID:SGTC_2517|Compound:totarol|FD-Score:4.79|P-value:1.01E-5 ID:SGTC_2765|Compound:myclobutanil|FD-Score:-3.65|P-value:6.42E-4 ID:SGTC_2793|Compound:7325131|FD-Score:3.73|P-value:4.46E-4 ID:SGTC_2849|Compound:9019321|FD-Score:4.12|P-value:1.22E-4 ID:SGTC_2855|Compound:9016525|FD-Score:4.05|P-value:1.56E-4 ID:SGTC_2887|Compound:9044784|FD-Score:-4.31|P-value:7.08E-5 ID:SGTC_2965|Compound:9082772|FD-Score:-4.47|P-value:3.88E-5 ID:SGTC_2974|Compound:9094440|FD-Score:3.60|P-value:6.71E-4 ID:SGTC_2976|Compound:9009005|FD-Score:4.59|P-value:2.21E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-4.08|P-value:1.59E-4 ID:SGTC_3225|Compound:9130819|FD-Score:5.70|P-value:2.04E-7 ID:SGTC_3289|Compound:9119912|FD-Score:4.16|P-value:1.08E-4

Top fitness defect scores for YLR310C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_434 1-hexadecyl-2-methylglycero-3 PC 15.80 8.0 µM 6.68E-45 1 2.07E-56 3.98
2 SGTC_1602 st001878 11.70 87.6 µM 1.07E-25 1 4.67E-32 6.72
3 SGTC_453 enantio-paf c16 9.40 10.0 µM 3.42E-17 1 2.77E-21 2.30
4 SGTC_1616 st002734 8.39 56.1 µM 4.34E-14 1 2.34E-17 2.42
5 SGTC_129 0088-0017 6.23 176.6 µM 1.49E-8 1 2.28E-10 2.13
6 SGTC_435 c-PAF 6.04 6.7 µM 3.87E-8 8 7.62E-10 0.42
7 SGTC_3225 9130819 5.70 49.5 µM 2.04E-7 amide catabolism 2 6.16E-9 0.76
8 SGTC_455 paf C-16 5.49 10.0 µM 5.26E-7 3 2.03E-8 0.80
9 SGTC_339 3091-4833 5.02 71.4 µM 4.02E-6 iron homeostasis 4 2.62E-7 0.25
10 SGTC_568 1193-0097 5.00 7.5 µM 4.24E-6 ERAD & cell cycle 17 2.80E-7 0.11
11 SGTC_701 4424-0219 4.86 185.0 µM 7.55E-6 calcium & mitochondrial duress 1 5.79E-7 1.07
12 SGTC_2517 totarol 4.79 10.0 µM 1.01E-5 2 8.32E-7 0.17
13 SGTC_227 fluconazole 4.74 33.5 µM 1.22E-5 azole & statin 38 1.06E-6 0.07
14 SGTC_2976 9009005 4.59 19.5 µM 2.21E-5 5 2.23E-6 0.65
15 SGTC_783 0848-0141 4.38 92.7 µM 4.89E-5 RSC & ERG11 12 6.03E-6 0.03
16 SGTC_1520 flindersine 4.33 88.0 µM 5.87E-5 8 7.58E-6 0.17
17 SGTC_555 4296-0685 4.26 37.0 µM 7.58E-5 ergosterol biosynthesis 4 1.04E-5 0.23
18 SGTC_2419 paclitaxel 4.23 166.5 µM 8.17E-5 4 1.15E-5 0.57
19 SGTC_145 3737-0113 4.22 38.4 µM 8.63E-5 DNA intercalators 42 1.23E-5 0.02
20 SGTC_3289 9119912 4.16 71.4 µM 1.08E-4 3 1.62E-5 0.38

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.265 6.74E-55 YDL210W UGA4 hom Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane
0.206 2.53E-33 YJL005W CYR1 het Adenylate cyclase, required for cAMP production and cAMP-dependent protein kinase signaling; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
0.181 3.64E-26 YHR155W YSP1 hom Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication
0.155 1.78E-19 YJL195C_d YJL195C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
0.152 7.58E-19 YDL134C PPH21 hom Catalytic subunit of protein phosphatase 2A (PP2A); functionally redundant with Pph22p; methylated at C terminus; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication
0.150 2.12E-18 YML101C-A_d YML101C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.146 1.54E-17 YDL035C GPR1 hom Plasma membrane G protein coupled receptor (GPCR) that interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis
0.140 3.45E-16 YER020W GPA2 hom Nucleotide binding alpha subunit of the heterotrimeric G protein that interacts with the receptor Gpr1p, has signaling role in response to nutrients; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.136 3.28E-15 YHR140W_p YHR140W_p hom Putative integral membrane protein of unknown function
0.132 2.13E-14 YLL007C_p YLL007C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL007C is not an essential gene
0.131 2.83E-14 YDR435C PPM1 hom Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits
0.130 3.86E-14 YJL076W NET1 het Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication
0.128 1.19E-13 YBR224W_d YBR224W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1
0.119 4.57E-12 YDL002C NHP10 hom Protein related to mammalian high mobility group proteins; preferentially binds DNA ends, protecting them from exonucleatic cleavage; likely component of the chromatin-remodeling complex INO80 complex; proposed to be involved in DNA repair
0.119 4.79E-12 YPR161C SGV1 het Cyclin (Bur2p)-dependent protein kinase that functions in transcriptional regulation; phosphorylates the carboxy-terminal domain of Rpo21p and the C-terminal repeat domain of Spt5p; regulated by Cak1p; similar to metazoan CDK9 proteins